LOCUS       sequence-341001-        8341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Rosa multiflora MYB108 for transcriptional activation 
            studies in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Dong J, Cao L, Zhang X, Zhang W, Yang T, Zhang J, Che D
  TITLE     An R2R3-MYB Transcription Factor RmMYB108 Responds to Chilling 
            Stress of Rosa multiflora and Conferred Cold Tolerance of 
            Arabidopsis.
  JOURNAL   Front Plant Sci. 2021 Jul 27;12:696919. doi: 
            10.3389/fpls.2021.696919. eCollection 2021.
  PUBMED    34386027
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-07-27"; volume: "12:696919. doi"; pages: " 
            10.3389/fpls.2021.696919. eCollection 2021"
COMMENT     Sequence Label: pGBKT7-RmMYB108
FEATURES             Location/Qualifiers
     source          1..8341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    705..752
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     777..796
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(984..1778)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(984..1778)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1095..1114)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1705..1724
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1779..1883)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1910..3252
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2873..2920)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3293..3311
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3349..3371)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3471..3490
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3511..3792
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             3793..4467
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(4570..5025)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4707..4728)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4919..4938
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5096..5800
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5828..6268
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     6279..6298
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6279..6297
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6317..6346
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      7442..7489
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      7516..7703
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7571..7595)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7727..7743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7727..7743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7740..7762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7751..7767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7775..7781,7782..7799,7800..7805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7820..7841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7958..7975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8129..8341,1..376))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8209..8228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
       61 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
      541 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
      601 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
      661 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
      721 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      781 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
      841 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
      901 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
      961 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     1021 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     1081 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     1141 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     1201 aagcaggcat cgccatgagt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     1261 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     1321 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     1381 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     1441 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     1501 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     1561 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     1621 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     1681 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     1741 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat
     1801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc
     1921 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     1981 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     2041 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     2101 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     2161 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     2221 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     2281 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     2341 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     2401 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     2461 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     2521 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     2581 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     2641 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     2701 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     2761 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
     2821 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
     2881 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
     2941 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
     3001 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
     3061 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
     3121 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
     3181 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
     3241 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3301 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     3361 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     3421 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     3481 gcagattgta ctgagagtgc accatagatc aacgacatta ctatatatat aatataggaa
     3541 gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca
     3601 gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg
     3661 gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa aggagagggc
     3721 caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag
     3781 gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt
     3841 tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg
     3901 ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt
     3961 tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg
     4021 tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc
     4081 gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt
     4141 attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa
     4201 cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat
     4261 tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt acattttatg
     4321 ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt
     4381 ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca
     4441 aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt taagtattgt
     4501 ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4561 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4621 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4681 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4741 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4801 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4861 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4921 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4981 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     5041 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagatcct
     5101 tttgttgttt ccgggtgtac aatatggact tcctcttttc tggcaaccaa acccatacat
     5161 cgggattcct ataatacctt cgttggtctc cctaacatgt aggtggcgga ggggagatat
     5221 acaatagaac agataccaga caagacataa tgggctaaac aagactacac caattacact
     5281 gcctcattga tggtggtaca taacgaacta atactgtagc cctagacttg atagccatca
     5341 tcatatcgaa gtttcactac cctttttcca tttgccatct attgaagtaa taataggcgc
     5401 atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc
     5461 gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc
     5521 aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc
     5581 ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt
     5641 acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca
     5701 attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt
     5761 ctgcacaata tttcaagcta taccaagcat acaatcaact ccaagcttga agcaagcctc
     5821 ctgaaagatg aagctactgt cttctatcga acaagcatgc gatatttgcc gacttaaaaa
     5881 gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg
     5941 tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga cagaagtgga
     6001 atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag aagaccttga
     6061 catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag gattatttgt
     6121 acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg agactgatat
     6181 gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag agagtagtaa
     6241 caaaggtcaa agacagttga ctgtatcgcc ggaatttgta atacgactca ctatagggcg
     6301 agccgccatc atggaggagc agaagctgat ctcagaggag gacctgcata tgatggatgt
     6361 caaaccagga agggtttcgg acatcattag aaacagttct gaggaagacg aagatgctat
     6421 gatgatggac ctgagaagag ggccatggac ggttgaggaa gacgttgttc tcatgaatta
     6481 cattgctaac cacggcgaag gtcgctggaa ctcccttgct cgctgtgcag gtctgaaaag
     6541 aactggaaag agctgcagat taaggtggct caactatctc cgacccgacg ttcgacgtgg
     6601 caacatcact ctcgaagaac agcttctcat tcttgagctt cattctcgct ggggcaaccg
     6661 atggtccaaa attgcacagc acttgcccgg aaggactgac aatgaaatca aaaactactg
     6721 gaggactcgc gtgcaaaaac acgcaaagca gctcaagtgt gacgtcaaca gcaagcagtt
     6781 caaggacacc atgcgctacc tctggatgcc aagattggtc gagagaattc aagccgccgc
     6841 gtccgccgcc accgccccca ccggagcccc ttcttctatc acaaccgccg cggccaacac
     6901 ttatcatttc aacaataaca ataacagctt ccaagccccc gcgggagcag gacacgtcgc
     6961 tttacaacag ccaacgccca ttggcaataa caatgacttg gccggaagtt acaccactcc
     7021 ggaaaactcc agcactgcgg cgtcatcgga ctcgtttggg actcaggtct cgcctgtgtc
     7081 agagctaacc gactattaca gtaatatgtc ggttaacaat aataaaccta gtagtcaggc
     7141 tctggattac ttccaagcca ccaatcctca tcaccaagtc ggttatcatg attccatgac
     7201 tagtccatct ggttactcat tcaatcaagg aggactaaac agccataact ttcaagctgc
     7261 ttcggcgccg gagcaaaaca acaacggcca gtggagcatg gacggtgggg acttttcgga
     7321 caatctgtgg aacgttgagg aatccgacat gtggttctta cagcagcaac tcggcaatat
     7381 ttgacatatg gccatggagg ccgaattccc ggggatccgt cgacctgcag cggccgcata
     7441 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc gcgcttgcag
     7501 ccaagctaat tccgggcgaa tttcttatga tttatgattt ttattattaa ataagttata
     7561 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
     7621 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
     7681 cttattgacc acacctctac cggcatgcaa gcttggcgta atcatggtca tagctgtttc
     7741 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7801 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7861 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7921 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7981 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8041 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8101 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8161 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8221 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8281 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8341 a
//
