LOCUS       sequence-341009-        7023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BMPR-2 lacking the kinase domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Aihara S, Fujimoto S, Sakaguchi R, Imai T
  TITLE     BMPR-2 gates activity-dependent stabilization of primary dendrites 
            during mitral cell remodeling.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109276. doi: 
            10.1016/j.celrep.2021.109276.
  PUBMED    34161760
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109276. doi"; pages: " 
            10.1016/j.celrep.2021.109276"
COMMENT     Sequence Label: pCAG-Bmpr2-Delta-Kinase
FEATURES             Location/Qualifiers
     source          1..7023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4045..4064)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4110..4165
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4164..4183)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4526..4542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4526..4542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4539..4561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4550..4566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4574..4580,4581..4598,4599..4604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4619..4640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4699..4894
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4745..4880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4900..5034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4937..4956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4991..5010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5102..5119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5273..5861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5353..5372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6032..6823,6824..6892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6655..6674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6893..6997)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg ggsgrggggs
      541 ggggsggggs grggcggrga ggkgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgccacca
     1741 tgacttcctc gctgcatcgg ccctttcggg tgccctggct gctatgggcc gtcctgctgg
     1801 tcagcactac ggctgcttct cagaatcaag aacggctgtg tgcatttaaa gatccatatc
     1861 aacaagatct tgggataggt gagagtcgaa tctctcatga aaatgggaca atattatgtt
     1921 ccaaagggag cacgtgttat ggtctgtggg agaaatcaaa aggggacatc aatcttgtga
     1981 aacaaggatg ttggtctcac atcggtgatc cccaagagtg ccactatgaa gagtgtgtag
     2041 taactaccac cccaccctca attcagaatg gaacgtaccg cttttgctgc tgtagtacag
     2101 atttatgtaa tgtcaacttt actgagaact ttccaccccc tgacacaaca ccactcagtc
     2161 cacctcattc atttaatcga gatgaaacga taatcattgc tttggcatca gtttctgtgt
     2221 tagctgtttt gatagtcgcc ttatgttttg gatacagaat gttgacagga gaccggaaac
     2281 agggtcttca cagcatgaac atgatggagg cggcagcagc agagccctcc cttgacctgg
     2341 atatggctga actcatgatg atatgggaga gaaacaagtc tgtgagccca acggtcaacc
     2401 caatgtcaac tgctatgcag aatgaacgca acctgtcaca taataggcgt gtgccaaaaa
     2461 tcgggcctta cccagattat tcctcttcct catatattga agactctatc catcatactg
     2521 acagcattgt gaagaatatt tcctctgagc attcgatgtc cagcacacca ttgacaatag
     2581 gagaaaagaa tcgaaattca attaattatg aacgacagca agcacaagct cgaatcccta
     2641 gcccagaaac aagcgtcaca agcctgtcca caaacacaac caccacaaac accaccggcc
     2701 tcactccaag tactggcatg accactatat ctgagatgcc atacccagat gagacacatt
     2761 tgcacgccac aaatgttgca cagtcaatcg ggccaacccc tgtctgctta cagctgacag
     2821 aagaagactt ggagactaat aagctagatc caaaagaagt tgataagaac ctcaaggaaa
     2881 gctctgatga gaatctcatg gagcattctc tgaagcagtt cagtgggcca gacccattga
     2941 gcagtaccag ttctagcttg ctttatccac tcataaagct cgcagtggaa gtgactggac
     3001 aacaggactt cacacaggct gcaaatgggc aagcatgttt aattcctgat gttccacctg
     3061 ctcagatcta tcctctccct aagcaacaga accttcctaa gagacctact agtttgcctt
     3121 tgaacaccaa aaattcaaca aaagaacccc ggctaaaatt tggcaacaag cacaaatcaa
     3181 acttgaaaca agtagaaact ggagttgcca agatgaatac aatcaatgca gcagagcctc
     3241 atgtggtgac agtaactatg aatggtgtgg caggtagaag ccacaatgtt aattcccatg
     3301 ctgccacaac ccagtatgcc aatggcgcag tgccagctgg ccaggcagcc aacatagtgg
     3361 cacataggtc ccaagaaatg ctgcagaatc aatttattgg tgaggatacc aggctgaata
     3421 tcaattccag tcctgatgag catgaacctt tactgagacg agagcaacag gctggccatg
     3481 atgaaggggt tctggatcgt ttggtagata ggagggaacg gccattagaa ggtggccgaa
     3541 caaattccaa taacaacaac agcaatccat gttcagaaca agatatcctt acacaaggtg
     3601 ttacaagcac agctgcagat cctgggccat caaagcccag aagagcacag aggcccaatt
     3661 ctctggatct ttcagccaca aatatcctgg atggcagcag tatacagata ggtgagtcaa
     3721 cacaagatgg caaatcagga tcaggtgaaa agatcaagag acgtgtgaaa actccatact
     3781 ctcttaagcg gtggcgcccg tccacctggg tcatctccac cgagccgctg gactgtgagg
     3841 tcaacaacaa tggcagtgac agggcagtcc attctaaatc tagcactgct gtgtaccttg
     3901 cagagggagg cactgccacg accacagtgt ctaaagatat aggaatgaat tgtctgtgag
     3961 cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     4021 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     4081 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     4141 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     4201 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     4261 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     4321 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     4381 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     4441 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     4501 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4561 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4621 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4681 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4801 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4861 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     4921 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4981 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     5041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7021 ctg
//
