LOCUS       sequence-341025-       13406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13406)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: pDEST51-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..13406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        473..1651
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          704..1642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1599..1619
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1668..1687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1668..1686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1718..1742
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9354..9378)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9404..9445
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9455..9472
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9495..9512)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9501..9725
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9771..10199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9858..9877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10068..10089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10208..10228)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10213..10542
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10393..10528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10455..10474
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10590..10637
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10656..11054
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11212..11333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11249..11268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11303..11322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11382..11398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11382..11398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11395..11417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11406..11422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11430..11436,11437..11454,11455..11460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11475..11496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11613..11630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11773..12361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11853..11872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12532..13323,13324..13392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13155..13174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(13393..13406,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttgatcaaca agtttgtaca aaaaagcagg
     1741 ctccgaattc gcccttatgg ctagtggcag ctgtcagggg tgcgaagagg acgaggaaac
     1801 tctgaagaag ttgatagtca ggctgaacaa tgtccaggaa ggaaaacaga tagaaacgct
     1861 ggtccaaatc ctggaggatc tgctggtgtt cacgtactcc gagcacgcct ccaagttatt
     1921 tcaaggcaaa aatatccatg tgcctctgtt gatcgtcttg gactcctata tgagagtcgc
     1981 gagtgtgcag caggtgggtt ggtcacttct gtgcaaatta atagaagtct gtccaggtac
     2041 aatgcaaagc ttaatgggac cccaggatgt tggaaatgat tgggaagtcc ttggtgttca
     2101 ccaattgatt cttaaaatgc taacagttca taatgccagt gtaaacttgt cagtgattgg
     2161 actgaagacc ttagatctcc tcctaacttc aggtaaaatc accttgctga tattggatga
     2221 agaaagtgat attttcatgt taatttttga tgccatgcac tcatttccag ccaatgatga
     2281 agtccagaaa cttggatgca aagctttaca tgtgctgttt gagagagtct cagaggagca
     2341 actgactgaa tttgttgaga acaaagatta tatgatattg ttaagtgcgt taacaaattt
     2401 taaagatgaa gaggaaattg tgcttcatgt gctgcattgt ttacattccc tagcgattcc
     2461 ttgcaataat gtggaagtcc tcatgagtgg caatgtcagg tgttataata ttgtggtgga
     2521 agctatgaaa gcattcccta tgagtgaaag aattcaagaa gtgagttgct gtttgctcca
     2581 taggcttaca ttaggtaatt ttttcaatat cctggtatta aacgaagtcc atgagtttgt
     2641 ggtgaaagct gtgcagcagt acccagagaa tgcagcattg cagatctcag cgctcagctg
     2701 tttggccctc ctcactgaga ctattttctt aaatcaagat ttagaggaaa agaatgagaa
     2761 tcaagagaat gatgatgagg gggaagaaga taaattgttt tggctggaag cctgttacaa
     2821 agcattaacg tggcatagaa agaacaagca cgtgcaggag gccgcatgct gggcactaaa
     2881 taatctcctt atgtaccaaa acagtttaca tgagaagatt ggagatgaag atggccattt
     2941 cccagctcat agggaagtga tgctctccat gctgatgcat tcttcatcaa aggaagtttt
     3001 ccaggcatct gcgaatgcat tgtcaactct cttagaacaa aatgttaatt tcagaaaaat
     3061 actgttatca aaaggaatac acctgaatgt tttggagtta atgcagaagc atatacattc
     3121 tcccgaagtg gctgaaagtg gctgtaaaat gctaaatcat ctttttgaag gaagcaacac
     3181 ttccctggat ataatggcag cagtggtccc caaaatacta acagttatga aacgtcatga
     3241 gacatcatta ccagtgcagc tggaggcgct tcgagctatt ttacatttta tagtgcctgg
     3301 catgccagaa gaatccaggg aggatacaga atttcatcat aagctaaata tggttaaaaa
     3361 acagtgtttc aagaatgata ttcacaaact ggtcctagca gctttgaaca ggttcattgg
     3421 aaatcctggg attcagaaat gtggattaaa agtaatttct tctattgtac attttcctga
     3481 tgcattagag atgttatccc tggaaggtgc tatggattca gtgcttcaca cactgcagat
     3541 gtatccagat gaccaagaaa ttcagtgtct gggtttaagt cttataggat acttgattac
     3601 aaagaagaat gtgttcatag gaactggaca tctgctggca aaaattctgg tttccagctt
     3661 ataccgattt aaggatgttg ctgaaataca gactaaagga tttcagacaa tcttagcaat
     3721 cctcaaattg tcagcatctt tttctaagct gctggtgcat cattcatttg acttagtaat
     3781 attccatcaa atgtcttcca atatcatgga acaaaaggat caacagtttc taaacctctg
     3841 ttgcaagtgt tttgcaaaag tagctatgga tgattactta aaaaatgtga tgctagagag
     3901 agcgtgtgat cagaataaca gcatcatggt tgaatgcttg cttctattgg gagcagatgc
     3961 caatcaagca aaggagggat cttctttaat ttgtcaggta tgtgagaaag agagcagtcc
     4021 caaattggtg gaactcttac tgaatagtgg atctcgtgaa caagatgtac gaaaagcgtt
     4081 gacgataagc attgggaaag gtgacagcca gatcatcagc ttgctcttaa ggaggctggc
     4141 cctggatgtt gccaacaata gcatttgcct tggaggattt tgtataggaa aagttgaacc
     4201 ttcttggctt ggtcctttat ttccagataa gacttctaat ttaaggaaac aaacaaatat
     4261 agcatctaca ctagcaagaa tggtgatcag atatcagatg aaaagtgctg tggaagaagg
     4321 aacagcctca ggcagcgatg gaaatttttc tgaagatgtg ctgtctaaat ttgatgaatg
     4381 gacctttatt cctgactctt ctatggacag tgtgtttgct caaagtgatg acctggatag
     4441 tgaaggaagt gaaggctcat ttcttgtgaa aaagaaatct aattcaatta gtgtaggaga
     4501 attttaccga gatgccgtat tacagcgttg ctcaccaaat ttgcaaagac attccaattc
     4561 cttggggccc atttttgatc atgaagattt actgaagcga aaaagaaaaa tattatcttc
     4621 agatgattca ctcaggtcat caaaacttca atcccatatg aggcattcag acagcatttc
     4681 ttctctggct tctgagagag aatatattac atcactagac ctttcagcaa atgaactaag
     4741 agatattgat gccctaagcc agaaatgctg tataagtgtt catttggagc atcttgaaaa
     4801 gctggagctt caccagaatg cactcacgag ctttccacaa cagctatgtg aaactctgaa
     4861 gagtttgaca catttggact tgcacagtaa taaatttaca tcatttcctt cttatttgtt
     4921 gaaaatgagt tgtattgcta atcttgatgt ctctcgaaat gacattggac cctcagtggt
     4981 tttagatcct acagtgaaat gtccaactct gaaacagttt aacctgtcat ataaccagct
     5041 gtcttttgta cctgagaacc tcactgatgt ggtagagaaa ctggagcagc tcattttaga
     5101 aggaaataaa atatcaggga tatgctcccc cttgagactg aaggaactga agattttaaa
     5161 ccttagtaag aaccacattt catccctatc agagaacttt cttgaggctt gtcctaaagt
     5221 ggagagtttc agtgccagaa tgaattttct tgctgctatg cctttcttgc ctccttctat
     5281 gacaatccta aaattatctc agaacaaatt ttcctgtatt ccagaagcaa ttttaaatct
     5341 tccacacttg cggtctttag atatgagcag caatgatatt cagtacctac caggtcccgc
     5401 acactggaaa tctttgaact taagggaact cttatttagc cataatcaga tcagcatctt
     5461 ggacttgagt gaaaaagcat atttatggtc tagagtagag aaactgcatc tttctcacaa
     5521 taaactgaaa gagattcctc ctgagattgg ctgtcttgaa aatctgacat ctctggatgt
     5581 cagttacaac ttggaactaa gatcctttcc caatgaaatg gggaaattaa gcaaaatatg
     5641 ggatcttcct ttggatgaac tgcatcttaa ctttgatttt aaacatatag gatgtaaagc
     5701 caaagacatc ataaggtttc ttcaacagcg attaaaaaag gctgtgcctt ataaccgaat
     5761 gaaacttatg attgtgggaa atactgggag tggtaaaacc accttattgc agcaattaat
     5821 gaaaaccaag aaatcagatc ttggaatgca aagtgccaca gttggcatag atgtgaaaga
     5881 ctggcctatc caaataagag acaaaagaaa gagagatctc gtcctaaatg tgtgggattt
     5941 tgcaggtcgt gaggaattct atagtactca tccccatttt atgacgcagc gagcattgta
     6001 ccttgctgtc tatgacctca gcaagggaca ggctgaagtt gatgccatga agccttggct
     6061 cttcaatata aaggctcgcg cttcttcttc ccctgtgatt ctcgttggca cacatttgga
     6121 tgtttctgat gagaagcaac gcaaagcctg catgagtaaa atcaccaagg aactcctgaa
     6181 taagcgaggg ttccctgcca tacgagatta ccactttgtg aatgccaccg aggaatctga
     6241 tgctttggca aaacttcgga aaaccatcat aaacgagagc cttaatttca agatccgaga
     6301 tcagcttgtt gttggacagc tgattccaga ctgctatgta gaacttgaaa aaatcatttt
     6361 atcggagcgt aaaaatgtgc caattgaatt tcccgtaatt gaccggaaac gattattaca
     6421 actagtgaga gaaaatcagc tgcagttaga tgaaaatgag cttcctcacg cagttcactt
     6481 tctaaatgaa tcaggagtcc ttcttcattt tcaagaccca gcactgcagt taagtgactt
     6541 gtactttgtg gaacccaagt ggctttgtaa aatcatggca cagattttga cagtgaaagt
     6601 ggaaggttgt ccaaaacacc ctaagggaat tatttcgcgt agagatgtgg aaaaatttct
     6661 ttcaaaaaaa aggaaatttc caaagaacta catgtcacag tattttaagc tcctagaaaa
     6721 attccagatt gctttgccaa taggagaaga atatttgctg gttccaagca gtttgtctga
     6781 ccacaggcct gtgatagagc ttccccattg tgagaactct gaaattatca tccgactata
     6841 tgaaatgcct tattttccaa tgggattttg gtcaagatta atcaatcgat tacttgagat
     6901 ttcaccttac atgctttcag ggagagaacg agcacttcgc ccaaacagaa tgtattggcg
     6961 acaaggcatt tacttaaatt ggtctcctga agcttattgt ctggtaggat ctgaagtctt
     7021 agacaatcat ccagagagtt tcttaaaaat tacagttcct tcttgtagaa aaggctgtat
     7081 tcttttgggc caagttgtgg accacattga ttctctcatg gaagaatggt ttcctgggtt
     7141 gctggagatt gatatttgtg gtgaaggaga aactctgttg aagaaatggg cattatatag
     7201 ttttaatgat ggcgaagaac atcaaaaaat cttacttgat gacttgatga agaaagcaga
     7261 ggaaggagat ctcttagtaa atccagatca accaaggctc accattccaa tatctcagat
     7321 tgcccctgac ttgattttgg ctgacctgcc tagaaatatt atgttgaata atgatgagtt
     7381 ggaatttgaa caagctccag agtttctcct aggtgatggc agttttggat cagtttaccg
     7441 agcagcctat gaaggagaag aagtggctgt gaagattttt aataaacata catcactcag
     7501 gctgttaaga caagagcttg tggtgctttg ccacctccac caccccagtt tgatatcttt
     7561 gctggcagct gggattcgtc cccggatgtt ggtgatggag ttagcctcca agggttcctt
     7621 ggatcgcctg cttcagcagg acaaagccag cctcactaga accctacagc acaggatcgc
     7681 actccacgta gctgatggtt tgagatacct ccactcagcc atgattatat accgagacct
     7741 gaaaccccac aatgtgctgc ttttcacact gtatcccaat gctgccatca tcgcaaagat
     7801 tgctgactac ggcattgctc agtactgctg tagaatgggg ataaaaacat cagagggcac
     7861 accagggttt cgtgcacctg aagttgccag aggaaatgtc atttataacc aacaggctga
     7921 tgtttattca tttggtttac tactctatga cattttgaca actggaggta gaatagtaga
     7981 gggtttgaag tttccaaatg agtttgatga attagaaata caaggaaaat tacctgatcc
     8041 agttaaagaa tatggttgtg ccccatggcc tatggttgag aaattaatta aacagtgttt
     8101 gaaagaaaat cctcaagaaa ggcctacttc tgcccaggtc tttgacattt tgaattcagc
     8161 tgaattagtc tgtctgacga gacgcatttt attacctaaa aacgtaattg ttgaatgcat
     8221 ggttgctaca catcacaaca gcaggaatgc aagcatttgg ctgggctgtg ggcacaccga
     8281 cagaggacag ctctcatttc ttgacttaaa tactgaagga tacacttctg aggaagttgc
     8341 tgatagtaga atattgtgct tagccttggt gcatcttcct gttgaaaagg aaagctggat
     8401 tgtgtctggg acacagtctg gtactctcct ggtcatcaat accgaagatg ggaaaaagag
     8461 acatacccta gaaaagatga ctgattctgt cacttgtttg tattgcaatt ccttttccaa
     8521 gcaaagcaaa caaaaaaatt ttcttttggt tggaaccgct gatggcaagt tagcaatttt
     8581 tgaagataag actgttaagc ttaaaggagc tgctcctttg aagatactaa atataggaaa
     8641 tgtcagtact ccattgatgt gtttgagtga atccacaaat tcaacggaaa gaaatgtaat
     8701 gtggggagga tgtggcacaa agattttctc cttttctaat gatttcacca ttcagaaact
     8761 cattgagaca agaacaagcc aactgttttc ttatgcagct ttcagtgatt ccaacatcat
     8821 aacagtggtg gtagacactg ctctctatat tgctaagcaa aatagccctg ttgtggaagt
     8881 gtgggataag aaaactgaaa aactctgtgg actaatagac tgcgtgcact ttttaaggga
     8941 ggtaatggta aaagaaaaca aggaatcaaa acacaaaatg tcttattctg ggagagtgaa
     9001 aaccctctgc cttcagaaga acactgctct ttggatagga actggaggag gccatatttt
     9061 actcctggat ctttcaactc gtcgacttat acgtgtaatt tacaactttt gtaattcggt
     9121 cagagtcatg atgacagcac agctaggaag ccttaaaaat gtcatgctgg tattgggcta
     9181 caaccggaaa aatactgaag gtacacaaaa gcagaaagag atacaatctt gcttgaccgt
     9241 ttgggacatc aatcttccac atgaagtgca aaatttagaa aaacacattg aagtgagaaa
     9301 agaattagct gaaaaaatga gacgaacatc tgttgagaaa aagggcgaat tcgacccagc
     9361 tttcttgtac aaagtggttg atctagaggg cccgcggttc gaaggtaagc ctatccctaa
     9421 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     9481 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     9541 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     9601 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     9661 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     9721 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     9781 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9841 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9901 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9961 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    10021 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    10081 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    10141 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
    10201 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10261 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10321 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10381 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10441 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
    10501 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
    10561 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
    10621 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
    10681 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
    10741 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
    10801 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
    10861 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
    10921 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
    10981 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
    11041 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
    11101 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    11161 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    11221 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    11281 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11341 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    11401 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    11461 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    11521 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    11581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc aggaaccgta aaaaggccgc
    11761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    11941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    12601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    12661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    12721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    12781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    12841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    12901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    12961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    13021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    13081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13381 ttgaatactc atactcttcc tttttc
//
