LOCUS       sequence-341032-        9042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alphaA-crystallin promoter (cry) drives mCherry expression in the 
            lens. The 600bp unc-45b muscle-specific promoter drives expression 
            of GFP-tagged zebrafish cct3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Berger J, Berger S, Li M, Jacoby AS, Arner A, Bavi N, Stewart AG, 
            Currie PD
  TITLE     In Vivo Function of the Chaperonin TRiC in alpha-Actin Folding 
            during Sarcomere Assembly.
  JOURNAL   Cell Rep. 2018 Jan 9;22(2):313-322. doi: 
            10.1016/j.celrep.2017.12.069.
  PUBMED    29320728
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.12"; journalName: "Cell Rep"; date: 
            "2018-01-9- 9"; volume: "22"; issue: "2"; pages: "313-322"
COMMENT     Sequence Label: pcry:mCherry,-600unc:cct3GFP
FEATURES             Location/Qualifiers
     source          1..9042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1057..1178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1301..2011)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(1381..1404)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1579..1598)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1848..1866
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2008..2017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2659..2675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2695..2715
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3318..3342
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4462..4464,4465..4467,4468..5178)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4462..4464,4465..4467,4468..5178)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4507..4528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4768..4787)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5115..5136
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5716..5740)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5742..5876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5939..5959)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5967..5983)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5999..6133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6572..6759,6760..6771)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6805..6821)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6805..6821)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6858..6878)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6858..6876)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6897..6913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6910..6932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6921..6937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6945..6951,6952..6969,6970..6975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6990..7011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7128..7145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7299..7887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7379..7398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8058..8849,8850..8918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8681..8700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8919..9023)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccattt acgccttaag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1261 aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac agctcgtcca
     1321 tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct
     1381 cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc agctgcacgg
     1441 gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct
     1501 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
     1561 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
     1621 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
     1681 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     1741 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
     1801 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
     1861 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     1921 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
     1981 ccatgttgtc atcctcgccc ttgctcacca tggtggcgac cggtaatgtc agacctggta
     2041 actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa cgcaagtagg
     2101 aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg
     2161 aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc
     2221 tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc
     2281 tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt ggccaactat
     2341 caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca tggctgtaga
     2401 acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc taattttttt
     2461 aaatatcaca gataatcaat cgcttttttt tttcttgact gaaaagctta gactggaaaa
     2521 ttctttgaaa aagtttgcac tacagaaaat aaatacataa catcctgcac tgaatgcaca
     2581 ctattaatag aaaagagagc attattgtag gaaatgtatt gtatatgtac attctgtgca
     2641 tttcacaatt ttctcgagca ggaaacagct atgaccatga ttacgccaag ctatcaactt
     2701 tgtatagaaa agttgccatt atcgtctccc tccagcacca cccagaaagt tgctcaatgt
     2761 tttgttacag tatgtgtgta acaccttccc ccccagccca gctctccact tttctcattc
     2821 tagaaagtac cagtcaactc tccaccagcc cagctgttgg cagacgcaca cctccatccc
     2881 cctgccctca gacatttgcc actgatttct cagctgtcat cccctctcca taaatagacc
     2941 ctatcagaga aagtccattg cactaatata aggggtgacc acatttctac aaaaccacaa
     3001 ttaatgtaca atcacaggaa taaacaaaca ctgcctaaac acacaatatc aagaacacac
     3061 aattacaatc tcaggttgtc attcgcaagc tgcgtagttc attcacaatg aacaggggct
     3121 taaaacaaaa gtcattccac ccctcaccat gaaatggatt catccgtaat cctggtatgt
     3181 taacgctcta tgtgtaagat atatataaga gtgactctta aagtgctgct actactattg
     3241 aacaaaccaa acgacaagaa acaacactga agcacagaac agattttctc ttacgacgaa
     3301 gctgaattga cttcaatgca agtttgtaca aaaaagcagg ctatgatggg ccgaccggtt
     3361 ctcgtgctta gtcagaacat caagagagaa tctggacgga aggtccagat tggaaacatc
     3421 tctgcggcta agaccatagc agacatcatc aggacatgcc taggaccaag ggctatgatg
     3481 aagatgctgc tggatcccat gggtggtatc gtcatgacca atgatggcaa tgccattttg
     3541 agagagatcc aggtccagca tcctgctgcc aagtccatga ttgagattag ccgcacacag
     3601 gatgaagaag ttggagatgg gaccacctct gtcatcattc tagcgggtga gatgctgtca
     3661 gtggctgagc agtttttgga gcagcagatg catcctactg tggtgatcgg tgcatacaga
     3721 caagctctgg atgacatgtt gaacatactc aaagacataa gcactccagt agatgtgagt
     3781 aatcgagaca tgatgttgaa gataataaac tctgcaatca acaccaaagc actcagccgc
     3841 tggtccacat tggcctgtaa catcgctctg gatgctgtcc gtactgttga gctggaggaa
     3901 aatggacgca aagagattga cattaagaag tatgccaaag ttgaaaaggt tcctggtggc
     3961 attatcgaag attcatgcgt gctgaggggt gtgatggtga ataaggatgt aactcatccc
     4021 cgcatgcgca gactcatcaa gaaccccaga attgttctgc tggactgttc tctggagtac
     4081 aagaagggcg agagccagac tgatattgag attgctcgtg aggaagactt cgcccgcatt
     4141 ctgcagatgg aggaagaata cgttcagcag atctgtgaag acatcattcg gctcaaacct
     4201 gacctaatct tcacagagaa gggcatttct gatctggctc agcactattt gatgaaagca
     4261 aacatcactg ctatccgccg tatcaggaag acggacaaca accgcattgc aagagcgtgt
     4321 ggtgcacgta ttgccagcag aaccgatgag ctcactgaag atgatgtggg tacaggagca
     4381 ggcctttttg aagtcaagaa gatcggcgac gagtacttca cctttgtgac agagtgtaaa
     4441 gaccccggat caggttcggg aatggtgagc aagggcgagg agctgttcac cggggtggtg
     4501 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4561 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4621 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4681 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4741 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4801 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4861 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4921 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4981 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5041 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5101 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5161 atggacgagc tgtacaaggg atcaggtaaa gcatgtacca tcctgctcag aggagccagc
     5221 aaggaaatct tggcggaagt ggaacgaaac ctacaggatg ccatgcaagt gtgtcgtaat
     5281 gtcctgctgg acccatatct gctgccgggt ggtggcgcag tggagatgga ggtgtcccat
     5341 cggctgacgg agcgctctcg agctatgact ggtgtggagc agtggccgta ccgcgctgta
     5401 gctcaggctc tggaggttgt ccctcgcaca ctcatccaga actgtggagc ttcagccatt
     5461 cgtgtgctca cttctctgag ggccaagcac actcaggagg gaaactcctc atggggtgtt
     5521 aatggagaga caggaactct tgctgatatg gagcagctgg gaatctggga accgctggct
     5581 gttaaagccc agacttacaa aactgcagtg gagacggcta tcctgttact gcgcattgat
     5641 gacatcgttt ctggccacaa gaagaaggga gatggagagc agacgggtgg agcaccgatg
     5701 gaggacagag agtagaccca gctttcttgt acaaagtggg ggatccagac atgataagat
     5761 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     5821 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     5881 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcca
     5941 actttattat acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga
     6001 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6061 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6121 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6181 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     6241 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     6301 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     6361 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     6421 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     6481 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     6541 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     6601 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     6661 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     6721 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     6781 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     6841 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     6901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6961 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7021 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7081 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7921 gctcagtgga acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc
     7981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8341 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8401 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8461 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8521 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8581 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8641 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8701 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8761 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8821 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8881 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8941 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9001 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
