LOCUS       sequence-341033-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alphaA-crystallin promoter (cry) drives GFP expression in the lens. 
            The 503bp unc-45b muscle-specific promoter drives expression of 
            GFP-tagged human ACTA1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Berger J, Berger S, Li M, Jacoby AS, Arner A, Bavi N, Stewart AG, 
            Currie PD
  TITLE     In Vivo Function of the Chaperonin TRiC in alpha-Actin Folding 
            during Sarcomere Assembly.
  JOURNAL   Cell Rep. 2018 Jan 9;22(2):313-322. doi: 
            10.1016/j.celrep.2017.12.069.
  PUBMED    29320728
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.12"; journalName: "Cell Rep"; date: 
            "2018-01-9- 9"; volume: "22"; issue: "2"; pages: "313-322"
COMMENT     Sequence Label: pcry:GFP,-503unc:hACTA1GFP
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1056..1177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1300..2013,2014..2016,2017..2019))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1303..2013,2014..2016,2017..2019))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2016..2025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2667..2683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2703..2723
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3226..3250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4386..4410)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4413..4987,4988..4990,4991..4999)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4580..4597)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4807..4826
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5000..5002,5003..5005,5006..5716)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5000..5002,5003..5005,5006..5716)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5769..5788)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5770..5788)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5793..5927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5990..6010)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6018..6034)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6050..6184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6623..6810,6811..6822)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6856..6872)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6856..6872)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6909..6929)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6909..6927)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6948..6964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6961..6983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6972..6988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6996..7002,7003..7020,7021..7026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7041..7062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7179..7196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7350..7938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7430..7449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8109..8900,8901..8969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8732..8751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8970..9074)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatt ggccttaaga tacattgatg agtttggaca
     1081 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1141 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1201 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1261 atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat
     1321 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     1381 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     1441 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     1501 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     1561 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     1621 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     1681 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     1741 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     1801 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     1861 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     1921 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     1981 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtaatgtcag
     2041 acctggtaac tccttactgt aacttattgc acctgatgaa caaaagatgt gaactgaacg
     2101 caagtaggag gctaatagag gctagtacag tacaaagatc cccaaatgat gtgtgccggc
     2161 cttagtggag gcagacccct ggttatatac ctgtgctgca gaaagagggg cttacaaatg
     2221 gatctctctg gaatggcatt atctcatggt gggaacagtg ttgtcagcag aatcagcggg
     2281 gtggactctg acctgagaat gacagtctag cactgaatgg ctcagacaat actcttgtgg
     2341 ccaactatca ccggaaaata agtgcctgcc aaaacaaacc agaccagatg aagctacatg
     2401 gctgtagaac gtcagtattt aatgtgttgt ggccacttgg ttcaccagat ttaagagcta
     2461 atttttttaa atatcacaga taatcaatcg cttttttttt tcttgactga aaagcttaga
     2521 ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg
     2581 aatgcacact attaatagaa aagagagcat tattgtagga aatgtattgt atatgtacat
     2641 tctgtgcatt tcacaatttt ctcgagcagg aaacagctat gaccatgatt acgccaagct
     2701 atcaactttg tatagaaaag ttgccattat cgtctccctc cagcaccacc cagaaagttg
     2761 ctcaatgttt tgttacagta tgtgtgtaac accttccccc ccagcccagc tctccacttt
     2821 tctcattcta gaaagtacca gtcaactctc caccagccca gctgttggca gacgcacacc
     2881 tccatccccc tgccctcaga catttgccac tgatttctca gctgtcatcc cctctccata
     2941 aatagaccct atcagagaaa gtccattgca ctaatataag gggtgaccac atttctacaa
     3001 aaccacaatt aatgtacaat cacaggaata aacaaacact gcctaaacac acaatatcaa
     3061 gaacacacaa ttacaatctc aggttgtcat tcgcaagctg cgtagttcat tcacaatgaa
     3121 caggggctta aaacaaaagt cattccaccc ctcaccatga aatggattca tccgtaatcc
     3181 tggtatgtta acgctctatg tgtaagatat atataagagt gactctcaag tttgtacaaa
     3241 aaagcaggct gagatgtgcg acgaagacga gaccaccgcc ctcgtgtgcg acaatggctc
     3301 cggcctggtg aaagccggct tcgccgggga tgacgcccct agggccgtgt tcccgtccat
     3361 cgtgggccgc ccccgacacc agggcgtcat ggtcggtatg ggtcagaaag attcctacgt
     3421 gggcgacgag gctcagagca agagaggtat cctgaccctg aagtacccta tcgagcacgg
     3481 catcatcacc aactgggatg acatggagaa gatctggcac cacaccttct acaacgagct
     3541 tcgcgtggct cccgaggagc accccaccct gctcaccgag gcccccctca atcccaaggc
     3601 caaccgcgag aagatgaccc agatcatgtt tgagaccttc aacgtgcccg ccatgtacgt
     3661 ggccatccag gccgtgctgt ccctctacgc ctccggcagg accaccggca tcgtgctgga
     3721 ctccggcgac ggcgtcaccc acaacgtgcc catttatgag ggctacgcgc tgccgcacgc
     3781 catcatgcgc ctggacctgg cgggccgcga tctcaccgac tacctgatga agatcctcac
     3841 tgagcgtggc tactccttcg tgaccacagc tgagcgcgag atcgtgcgcg acatcaagga
     3901 gaagctgtgc tacgtggccc tggacttcga gaacgagatg gcgacggccg cctcctcctc
     3961 ctccctggaa aagagctacg agctgccaga cgggcaggtc atcaccatcg gcaacgagcg
     4021 cttccgctgc ccggagacgc tcttccagcc ctccttcatc ggtatggagt cggcgggcat
     4081 tcacgagacc acctacaaca gcatcatgaa gtgtgacatc gacatcagga aggacctgta
     4141 tgccaacaac gtcatgtcgg ggggcaccac gatgtaccct gggatcgctg accgcatgca
     4201 gaaagagatc accgcgctgg cacccagcac catgaagatc aagatcatcg ccccgccgga
     4261 gcgcaaatac tcggtgtgga tcggcggctc catcctggcc tcgctgtcca ccttccagca
     4321 gatgtggatc accaagcagg agtacgacga ggccggccct tccatcgtcc accgcaaatg
     4381 cttctagcca gctttcttgt acaaagtggc cgcccctctc cctccccccc ccctaacgtt
     4441 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4501 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4561 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4621 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4681 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4741 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4801 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4861 cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg
     4921 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4981 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5041 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5101 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5161 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     5221 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5281 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5341 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5401 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5461 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5521 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5581 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5641 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5701 ggacgagctg tacaagggcg gtggaagatc tgggaattca aggcctctcg agcctctaga
     5761 ttctgcagcc ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     5821 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5881 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5941 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcc aactttatta
     6001 tacatagttg ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat
     6061 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     6121 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     6181 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     6241 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca
     6301 gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg
     6361 ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata
     6421 atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc
     6481 acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca
     6541 taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt
     6601 aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa
     6661 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
     6721 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
     6781 tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct
     6841 tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc
     6901 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     6961 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7021 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7081 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7141 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7201 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7261 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7321 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7381 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7441 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7501 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7561 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7621 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7681 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7741 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7801 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7861 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7921 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7981 aacgaaaact cacgttaagg cattttggtc atgagattat caaaaaggat cttcacctag
     8041 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8101 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8161 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8221 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8281 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8341 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8401 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8461 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8521 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8581 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8641 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8701 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8761 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8821 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8881 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8941 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9001 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9061 ataggggttc cgcgcacatt tccccgaaaa gtg
//
