LOCUS       sequence-341034-       21502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alphaA-crystallin promoter (cry) drives GFP expression in the lens. 
            The acta1 muscle-specific promoter drives expression of GFP-tagged 
            zebrafish dmd..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Berger J, Li M, Berger S, Meilak M, Rientjes J, Currie PD
  TITLE     Effect of Ataluren on dystrophin mutations.
  JOURNAL   J Cell Mol Med. 2020 Jun;24(12):6680-6689. doi: 10.1111/jcmm.15319. 
            Epub 2020 Apr 28.
  PUBMED    32343037
REFERENCE   2  (bases 1 to 21502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/jcmm.15319"; journalName: "J Cell Mol Med"; date: 
            "2020-06"; volume: "24"; issue: "12"; pages: "6680-6689"
COMMENT     Sequence Label: pcryGFP-acta1-dmdGFP
FEATURES             Location/Qualifiers
     source          1..21502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(169..190)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(539..550,551..713)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1081..1202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1325..2038,2039..2041,2042..2044))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1328..2038,2039..2041,2042..2044))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2041..2050
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2692..2708
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2728..2748
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6705..6729
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6730..6739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             12795..12803
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             join(17482..17484,17485..17487,17488..18201)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(17482..17484,17485..17487,17488..18198)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(18201..18225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    18227..18361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(18424..18444)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(18452..18468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    18484..18618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(19057..19244,19245..19256)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(19290..19306)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19290..19306)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(19343..19363)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19343..19361)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(19382..19398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19395..19417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19406..19422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19430..19436,19437..19454,19455..19460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19475..19496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(19613..19630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19784..20372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19864..19883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(20543..21334,21335..21403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21166..21185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(21404..21502,1..6))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
       61 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      121 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      181 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      241 ggcgatggcc cacgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
      301 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
      361 aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac
      421 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
      481 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
      541 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
      601 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
      661 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
      721 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
      781 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
      841 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
      901 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
      961 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     1021 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agattggcct
     1081 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1141 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     1201 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     1261 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
     1321 cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     1381 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     1441 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     1501 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     1561 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     1621 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     1681 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     1741 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     1801 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     1861 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     1921 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     1981 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     2041 ccatggtggc gaccggtaat gtcagacctg gtaactcctt actgtaactt attgcacctg
     2101 atgaacaaaa gatgtgaact gaacgcaagt aggaggctaa tagaggctag tacagtacaa
     2161 agatccccaa atgatgtgtg ccggccttag tggaggcaga cccctggtta tatacctgtg
     2221 ctgcagaaag aggggcttac aaatggatct ctctggaatg gcattatctc atggtgggaa
     2281 cagtgttgtc agcagaatca gcggggtgga ctctgacctg agaatgacag tctagcactg
     2341 aatggctcag acaatactct tgtggccaac tatcaccgga aaataagtgc ctgccaaaac
     2401 aaaccagacc agatgaagct acatggctgt agaacgtcag tatttaatgt gttgtggcca
     2461 cttggttcac cagatttaag agctaatttt tttaaatatc acagataatc aatcgctttt
     2521 ttttttcttg actgaaaagc ttagactgga aaattctttg aaaaagtttg cactacagaa
     2581 aataaataca taacatcctg cactgaatgc acactattaa tagaaaagag agcattattg
     2641 taggaaatgt attgtatatg tacattctgt gcatttcaca attttctcga gcaggaaaca
     2701 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc acgcgcagat
     2761 tccgtgtggg cctggtaata tcttaccatt ttaagctatt ttaagattag ggaaaaaaat
     2821 taaataaaac tactttaaaa ttactttcct taaaaagcaa catatttatt tagtttttgt
     2881 ttaactggct caagattgta atattttact tttacatgtt tttttaagta cctaaatgtg
     2941 ttcaaaagta aagtatgtta ttgttttttt tttttaatgt tagctaagct ttaaattgta
     3001 atatattact tttaaaagtg actttttaaa aatgtaaata aaaaagtaac tataaatgtg
     3061 taatgcatgt tatttctgtg ggtttttaat tttcatctca actgggctta caattttaac
     3121 ataataaaaa tcaaatcaaa tcaaatcagg cccttatttg tcacatacac tcttgtatac
     3181 tgaaataaac taaatgttga gaaaaattta aatacaccaa aataacgcat accttttttg
     3241 taattaccta agtaaaacat ttagttcctt ttttaagtga ctcaagattg taatctatta
     3301 attgtaaaag ataaaactag tgatcaatct gacgtgcttc tatgcagcct gtgagattaa
     3361 ttgtgatgtt taattattaa acaatttaaa tggatcaaac atttcacatg tgcttattgt
     3421 gtccaacaaa accagcagaa gcacttaaaa atattgcatg acaacatgag tttttaatgt
     3481 aaattatata tacttcctaa acgaagttaa tcatatttct aaaataataa tgtcattgaa
     3541 atatttaaat aaatgataag ctttagttga ttcaacataa aattgaaagc aataaggact
     3601 tttttttttt acagcggtga agcaaaagca tgtaataatc atataatata ataaataaat
     3661 aaataaataa tggctcttgg tgcaaatatg aacactttaa ataaatatag aataaatatg
     3721 atcaataagt cataacgatg tgttttatgt tgattataat ttaaacatgt ttctaacgca
     3781 agttttaaaa actgttttaa agtattattt taagttataa gatcaatttg actcaacatt
     3841 ttgtagtgaa aatatgttgt gtaaaattaa tataatatac actataaaaa tgcagggttc
     3901 cacacaatac attaatgttg tcccaacaca aatcgattaa gttaacaaat atatgtggat
     3961 tgaacataaa aaattaagtt gtcccaatga aatctcaaga attgtgttgt ttcagctcat
     4021 tttaaataag tagttttaac aagtaaaaaa taacatattt ttgagtgtgt atatatatat
     4081 attacaaaat ataataatat aaaatgtaaa agatataaac catgctacat atatttaaga
     4141 gctattttgc acattacttt tttgcaagcc attttagtgt tttttttaca aatatatatt
     4201 agctaaatat acaatatctt aaatatacaa ttaatcagac atgcaattaa tgcatttaac
     4261 ctacataaaa atacatgatc aacatatgtt ttatgctgat tacaactaaa tcaaacgtaa
     4321 tgaaattaat ttataaaagc gatttaaata tgtcaatatt ttaagttaaa atcacttgaa
     4381 acctactata aatgaactta aattaaaaaa atctccttta attccaatgc aacagtgttg
     4441 ttttttcagc tgaggacccc cggtgcagat ggttggcccc cggcccgcta ttctaaggaa
     4501 gcctttctta attttagggc ggcgctgcat gcgctcagat cagattcacg ggtggagagg
     4561 gttattttct cttttttatt ttttcttgcc tgctcatatt tgggcctgtg tgaaccccag
     4621 gctccgcctc ggcccccagg agccgctctt atttaaaggg gatcctcgcc ttcagtcttc
     4681 gtcccagtgg aagcatcagt cttgtgataa caacttggtg agtcctacag accagcgctc
     4741 gactctaggc tttaaatgca ttgatttctc tggattagct accgggagag ttttaacttt
     4801 gcttattagt cttgtgctcg aactgctagg tttaaagagt gttttctcta atgcatgcat
     4861 gaagcgacag ctgaaaacca gaaatagagc gtttgtcctc ctgttaaact ctagcctttc
     4921 tttggcattt gtcagtccga gtcttcagct gccggcagga gattgggttt cagtaacact
     4981 tgctgagcac aaactacacg tgctttcttc agatttaatt cattattaat cattctagat
     5041 ttaaatgatg ttcttactca ttgctgatta ttttagcctt gcggagcaga ttaaatgagc
     5101 acgtgtttca ttaagattga tttaaatata attaatattt caccactgta gttttaaaat
     5161 tgccttctaa gtcatggttg atttttaata ggtatcggct ctaattagat tactattttg
     5221 gtttttaaat gttatttcta tttcatttgt aggctcgttt ttaatgtgat tttattagat
     5281 tctagtttta aaatcatctc ttagccttaa tgagcagact gaatgaacat gtttcattaa
     5341 gattaacttc attataatga attatttaat cattctagtg ggttataaat cagctctatt
     5401 taaattaatt gtcgtgttta tttctattta atttttgatt attttttcag ggtcgttttt
     5461 atgtgatttt ttcccgtttc taattttaaa ctaattgttt gatttttata tgattcgact
     5521 ctttttaaat gaattgtttt gcttttttta ttgagcatat atatattata caatttcaat
     5581 caacaattgt tgtgcttttt aaatgttgtt tctatttcat ttctagggtc gattttattc
     5641 gattctagtt ttaaaatcgt gatttttaac ttctaacctt actgagcaga ctgaatgaac
     5701 atgtttcatc aagattaact tcattatagt taattattga atcgtgctag tggtttataa
     5761 atcagctcta tttaaattaa tcatcatcat tatttctatt taatttttat tatttcttta
     5821 ggattatttt tatgtgattt ttttcacgtt ctagttttaa actcaatgct cattactgct
     5881 cacccttgct gagcagtctt tcgttaagat taaattaatt aataaccatt ctagttttaa
     5941 aacagtgtct taaggcatag ctgatatttt acaagtttac tatttaatat atctcctttc
     6001 tttttttagg gtcgatttca tctgatttga ttaattttta ttatcttcac taagcttttt
     6061 gttctaggtt ttattctcag tattattact gttatatttg aactgaattt ctcctcatag
     6121 tttattcatt ttatttattt attttttgtt tattcgctat tttgtatttt agtttttaat
     6181 tagaaatttt attagaatcg ttttgaacta gtttttatgc actctaacat taatattaaa
     6241 tcataactat tatttttatt ataatttatt tgtgtttatc tttttgtgta gctttcatgt
     6301 gtttttattt tagttttatt atgtttgcat tgaatttttt ggactagttt atataacgat
     6361 atacagaaaa gccatgacaa caaatgtttt tataatacct attaagtata agctaatcag
     6421 atttccacta ttctgttcaa agttaacatt attagccatt ctgatggatc atttgacatt
     6481 tgtcatatgt ctttatgaac actgctcttt cagatgccac ttttaaagct gttttttaca
     6541 gtaaatgaac gtttattatt atgacattat ttcctaaggc ctctagagta cagcaggatg
     6601 tggcgtgtgc attaccgtgt aattatgctg actgtaaatg tgcatttata gactgtcagc
     6661 atgtctgact gtgcgctgtt gtgattggtc ctgcacagac caaggcaagt ttgtacaaaa
     6721 aagcaggctg ccgccatggc cgaagcagtg aacccacagg accaatggga ggaaggtttt
     6781 gaggacgaat tcggagaaat catcaaaact cgatcagatg aaagagaaga cgttcaaaag
     6841 aaaaccttca ccaaatggat aaactcacag tttgctaaga caagaagacc tcctattgat
     6901 gacctcttca ctgacctgtg tgatggccga cgtcttcttg aacttctgga ggttctggtt
     6961 ggccatgaaa tagttaaaga acgtggcttc actcgagtgc actcccttaa caatgttaac
     7021 agggccctgc agatccttca gaagaacaat gttgatctgg tgaacattgg aggagctgac
     7081 attgtagatg ggaatcataa actgaccctg gggctcatct ggagcatcat tctccactgg
     7141 caggttaagg atgtgatgaa agatgtcatg gcggacttac agcagaccaa cagcgaaaag
     7201 atcctgttaa gctgggtcag gcagtccctc aaaaactacc aagacgtcaa cgtggtcaac
     7261 ttctccagca gctgggccga cggtttcgct ttcaacgctc tcatccacag ccacaggccg
     7321 gagctgttca gctggagtgt agtggagcaa caggataatg ccattgagag actggatcat
     7381 gccttcggtg ttgcggagaa gagtttagga atcgaccggc tgctggaccc cgaggatgtt
     7441 gctacagtcc atccagataa gaaatccatc atcatgtacg tcacctccct cttcaaagtg
     7501 ctgccccatg gcgtgagttt ggaggccatc caagaggtgg agaccctccc tcgagccgca
     7561 gtgaccaagg aggagcacgt cctctatcaa acccaacagc ggtactctca gcagatcaca
     7621 gtcagcgtgg cccagagtcg cgttcgcagc ccgtcgccct cctataagcc gagatttaaa
     7681 agctatgcct tcactcaagc cgcctacgtc aagacacctg aacagcagag gaagttcctc
     7741 atcgcacagt ctccagacaa ggccgatgaa ctgcgaccgt cccccagtcc cctgccgcag
     7801 gggctaaatg agctggaaag ctaccagagt gccctggagg aggtcctgac ctggctgctc
     7861 tctgcggagg acggcctgca agcacagcca cccatctctt cttttgtaga agaagtcaaa
     7921 gagcagtttc acactcatga gggctacatg gtggagctga cgtctcacca gggcagtgtg
     7981 ggccgggtcc tgaaagcggg aagtgtgttg ttgtctggag ggcagctgac tgatgatgaa
     8041 gagagggagg tgcgggaaca gatgaatctg ctcaactcgc gctgggaaca tctgcgtgtg
     8101 gccagtatgg agcgacagag caggcttcat gaagttttga tggaccttca gcaccagcag
     8161 ctgaagcagc tctctgattg gctggacacg acggagactc ggattaaaag gatgggagcg
     8221 caggctttgg ggcctgaact tgatgacata aagcggcaaa tcgaagagca gaaactctta
     8281 caggaagatc tggagctgga acaagtgcga gtgaattcgc tgactcatat ggtggtggtt
     8341 gtagatgaga atagtgggga tggcgccacc gcagccttgg aggaaaagct gcagaacctt
     8401 ggggaacgat gggcagcgat ctgtaaatgg actgaagagc gttggatcct ccttcaaaag
     8461 atcctgctgt gctggcagca tttctctgag gagcagctgc tgtttgattc ttggctgact
     8521 caaaaggagg agttggtcca gtccatccaa agcagtggca caaatgaccc aaatgaagta
     8581 gccgccaacc tgcggaagct ggcgatttta aaagcagacc tggagctgaa gaggcagacc
     8641 atggacaagc tttgctcgct cgtccaggat ctgctgacta acatcaagag caaagaggcc
     8701 gctgggaagc tggaagcaaa gctggagagg tttgctcagc gctgggacaa attggtgcag
     8761 gcgcttcagc tcaccagcac caagatttca actattgtca ccacatccca gtcggagatc
     8821 acgcacacaa ccatggcaac tgtcaccaag gtgaccacga accagaagaa gatggtgaag
     8881 catactaagg agggcatgtc cacccctcca cctcagaaaa agagacagat tgttgtggat
     8941 tcagaactgc ggaaaagatt tgatgtggac ttcactgaaa ttcacagttt tatgactcgg
     9001 tccgaggctg ttttgcaaaa tcccgagttc tcaatctccc gtaaagaagg cagtgtggca
     9061 gatctctatg aaaaagtgct ggcaattgac agggaaaaac ccgaaaagtt caggaagttg
     9121 caagaagcca ctcgctcagc ccaggctttg gtggatcaac ttaccagcga cggtcagaac
     9181 tcagaggaca tccagcaggc ggctcagcag ttacgtgctc gatgggtgga cttctgcgct
     9241 ctcctggccg agcggctggc gtggttggcc taccagacca aagtgctggc tttctacaat
     9301 ctctttcagc agctggagca ggctgcggcc acggcggaga actggctgaa ggtgcagtcg
     9361 ccgcccgcat gtgaacccga acctctgagg atccagctgg agcgctgcag ggatgaaatc
     9421 tctcgcctct cggctcttca acctcaagtg gccaaactgc acgaacaact cgaggagctg
     9481 cggcagaaag aagagacgcc ggttctgttt gacgcggaca tttctgcctt ccaagagcac
     9541 taccaccaag tcctggagga cctgagggcc agggaacgac agctcgtttt ggtccagtct
     9601 agtcttcctc cagcacgtta taaggatgtg atggcagcgc tgttggcttg gctacagcag
     9661 tgtgagaata aactagccat cccttctaca gcagtgactg agtatcccgt catggagcag
     9721 agactcaaag atatcaaggc aatccaagca tcccagaagg agcatcaggg tgacgtggat
     9781 gacctcaata agatggctga gcaggttttc cagaaagctc cacctgagat ctgtcagaag
     9841 taccggacgg agttggataa cgtgatggtg cgctggagac gcatatcgga gcagctggag
     9901 gagaacatcc agaaactgca ggatcacatg accaagctac agcagttcca gaatgacaca
     9961 aagactctgc agaagtggat ggcagaggtg gatgttttcc tgaatgagga atggccagct
    10021 ctgggagacg cagaggcttt ggagaaacag ttggaacagt gcacggctct ggtgaatgac
    10081 atccacaccg tccaacccag tctgaacggc atcaatgagg tgggactggc cttgaagagg
    10141 gaagctgaga cgccgtttgc catcaaaata cagaagatgc ttgatgagct caatgcgcag
    10201 tgggagctca tttgcaaaca ggcttatgca aagaaatcag ccctgaaagg agggctggac
    10261 atgacggtga gtttgaggaa ggagatgcag gagatgcagg agtggatcac tcaggccgag
    10321 gaagagtacc tggagagaga cttccagtac aaaacacctg aggagcttca taaagcagtg
    10381 gaggagctga agcgggctca agaggaagtc catcaaaagg aaacaaaggt gaagctgctg
    10441 acggataaag tcaccaattt catttcaaag gctcctcccg cggcccatga tgccctgaag
    10501 gcagaactgg atgttttgac ttctaactac cagcgcctgt gcagccgact cgatggaaaa
    10561 tgcaagactt tagaggaagt gtgggcatgc tggtgtgagc tgctgtctta tttggagttg
    10621 gagaatgcct ggatggacct acttgaaaag aaacttgatg aaacggaagg gcttcaagga
    10681 ggcatagagg aaattgaaga agctttgact tctttagaca ccatgattag agagcatcct
    10741 gaatacaacc gtaaccagat acgtgaatta gcgcagaccc tgatggatgg ccgagtcctg
    10801 gatgagctca tccacaaaaa ggtggaggac tacaacacac gctgggatga actgatgcaa
    10861 agggcttcac aaaggcgcca gcagctggag aagagcttgc attgggccca ggagaacgac
    10921 aaaaccctgc gtctcattca ggactctctg aacaccactg accgacatct gaccgcttac
    10981 atagcagatg gcatagacgc tgcacagata ccacaggaag cacagaaaat tcagactgag
    11041 ttgaacggcc atgaggtgac actggatgac atgaagaaaa aggctatgga ggttgacgcc
    11101 tcagagaaag tgattggaga gatcgatgca acattagata aacttttgca agtaaagggc
    11161 aagttccggc ttttccaaaa gccagcaaac tttgaccagc ggctgaggga gtgtgagcga
    11221 gtgctggagg aggtgaaagt gaagctggga gtgctgagta ttcgcagtgt ggagcaggaa
    11281 gtggtgcagt cacagcttga gcagtgcatg aaattttata agaacctgag tgaggtgaag
    11341 tctgaggtgg agacggtgat aaagacgggg aggcagattg tgcagaagca gcagacggag
    11401 caacccaagg agttagacga caggctgacg gccctcaaac tcgcttacaa tgatttgggt
    11461 tcacaggtaa cagaaggaaa gcaggaactg gagaagattt tgaagttact gaggaagttt
    11521 aggaaggagg tgaacagtct aaccgaatgg ctggctacta ctgatgggga actgacgcgc
    11581 cgctcatcgg tagagggtat gccaagtgat ttggatgcgg agttggcttg ggcaaaggca
    11641 gcgcaggagg acactgagca tcataagcca cagttgaaac tggtgaggga gttggcaggg
    11701 accctgaagg gtttacttca tagccaagag aacctgattg atgacaaggt cagtttgctg
    11761 aactgcaact ggatcgcagt gacgtcacgg agcgaacagt ggctcaaact cctgctggat
    11821 tatcaaaatc aaatgaaaac acttgagcag aacatttctc agatcaacac ctggatggat
    11881 cgcgcagaag agaagctgga tgagatggac agccagggct gcattgagca cgttataaag
    11941 ggtttgcgaa tggagttgga ggagatgaag gggaaagttg aagcagtgca agttctagca
    12001 gaggatctta taaagaacac aggggaacat tgtaaagctc aagtgaaacc aaagcttgaa
    12061 cagcttaacc aacgttttga cattgttgca agaaggattt taatgggaca ggcctcctcc
    12121 caagagttag acgagtacca cagacaggct aatatatggc tccaggtgct ggacgaggag
    12181 attaaattag gtgaaagtct aaaagaggaa gatttcctgg aggatgcgac tatagacgag
    12241 gaggctctaa atgaactgtt cctaaaaggg gagaatctcc tcaagagaac tcccggtgga
    12301 gagaaacgag aggccgttcg agagaaacac aaccttctcc atgacagata tgacactcta
    12361 aagaatctga gagccctgag aaagaagaag gctctggctc tcgcatcgca gtggtatcag
    12421 ttctgcaaga aaagcgatga catgatgcag tggctggaca gaatcgagaa aagcatacga
    12481 gagcttccag atcccccgga ggagcccaga gtgaaagcag ttggatatga gattgaccag
    12541 cagcggccca agctggagga tttacgtgga ttggggcgtg tgctttcaga gggtggagct
    12601 agcagaatgg ttgagccccg cctcctgcct atcaataaac gatggacgga gctagacgtt
    12661 aacttcactc gagtgcgcca caaaagtgag ctgcagtttc tgctgcagtg tatctctgag
    12721 aatgaggctc tgctgaactc tcccgagcgc tggtctactg cattcatcaa ccttccccaa
    12781 caagagaagt gccttaagga ggttaaagtt aacctggata aactggaggg tcccgtggca
    12841 gaggctgttg gaagaggtgc atctcaacct gaggagggtt tgctggttca gctgctcagg
    12901 accaactggg aaaaccttaa aatgctctac caggacagac tcaaacgcct tgaaaaagcc
    12961 aagaagttca atgaggagct gaaaatgctg gacaactggc tcacagatgc tgagaggacg
    13021 attatgaagt atgaacaaga ccccataaat aatagagacc acctcaagga gcttcaggcg
    13081 ggtttggaga aacaggaagc agcagtgaag ggactcaatg ctcttgggac tgatctgagt
    13141 ccacagtgca gtaaagacga cagggaccac attaaacagc agctcgcctc gatcaactcc
    13201 cgctgggcca aagtgtccaa ccagctcact gagatcaaga gacggtctgc cggggcaaaa
    13261 atgcttctcg cagagttaaa tgaagacatg ggggagtttc aatcctggct ggatgatgcg
    13321 gaggctgtgg ctgcacttcc tgtggaagcg ggtcgcaagg aacagctcag tgcaacttta
    13381 gaaaaagtca aggcccgggt ggtggaactg ccgagcagga agcaagctct tcatcagatc
    13441 aacagcaaag gctcatctct ccccgctgat aaagtcaaac cattggagaa gcaactaaag
    13501 gttatcaaca tgcgctgggc gaaggtgtcc actgatcttc ctgagaagca gagacaaatc
    13561 gaggatctcc tcagggattt gtctttgtac caagaacagc tctccaaact ctcaatttgg
    13621 gcctccaata caaagactca gcttgagcag tcgcctactg cagtggaccc aaagattgag
    13681 gatgacgtaa aagaaaagaa gccagctgtg gaaacccttt tggcaaaaga gagaccgccg
    13741 tgccagcctg agaagggcca gtatgacggt ctgagtgcag attggacatc cattcaagtt
    13801 ctccttaaag actggaaaga caaatgccag ctcgcagctg taactttgac tggtagtgca
    13861 gcaggagatg cggctctcga caaattcaac aaatcctgga cagagcttga cgattggttg
    13921 gcgctgctgg atcacatggt tcagacccag agagtaatgg tgggggatct ggacgagatc
    13981 aacgaaatga cggtcaaact caagtcagca ctgcaagaca tggagcaaag atgcccacag
    14041 ctgaacaaac aaatcactgc ggctcagaac ctgaagaaca aaaccaataa ccctgagaca
    14101 cgggcaacta ttacagaccg cattgagaag ttacaggcac attgggaaga ttctcatgcc
    14161 aagctgacgg ccagagttct tacactgcag aacatgtata aggactccag cgattggttg
    14221 gaggccagaa aaagggtgga gcctctcatt aagaaagcca atgaaaaact cgagagctgg
    14281 aagaaagttt cgcacagcgt tgaagattta aaaggccaaa atgcagatgt caagcaactt
    14341 tctaaggatc tacagcagtg gcagactcag atgaatgtca ccaatgagct ggcaaacaaa
    14401 ctactgactc tttatgcaga cgacgacaca agcaaagtga aacaaatgac cgagagcatg
    14461 aatctggctt gggccaacat caaaaagcgt gcaggggaca aggaggcaga tctggaagct
    14521 ggacttcggc agttgcagca ttactacttg gacctggaga aattccttaa ttggctaacg
    14581 gaggcagaaa ccacagccaa tgtcttacaa gatgccacct ttaaggaggg acttctggaa
    14641 aatcctgcca cagttcgaca tttactcgag caatggcagg atctccaggc agagattgat
    14701 gctcaccggg agacgtacca ttcattagat gaaaacggac atcgtattgt gtcgtctctg
    14761 gagggaacgg acaatgctgt ggtgctacaa aaacggcttg atgacatggg gcagcgctgg
    14821 catgagttgt gcaacaaagt tatgagtata aggccctatc tagatgccgg cgttgatcag
    14881 tggaaacact tgcacatgtc cttacaagag ctgctcaact ggctgcagct gaagagggaa
    14941 gagctggaga agcagaagcc agtagggggc gacgtgccga ccgttcacca acaactcctc
    15001 acgcacaagg ctttcagaag agagctgggt gccaaagaac ctgtaatcaa tggaactctt
    15061 gacaatgcga aaaccttcct cgctgagatg cctcgtgagg gcctgaaaca gagacctgga
    15121 caaaaagatg taagtcctga ggagcgggtt caaaacgtgg gccgcatatt gcgtaaagaa
    15181 gtggaggatg taacggtgcg atggaagaat ctgggtgcag cttctgtgga ctggcagcaa
    15241 cagctggaac tggccttgga gaggctgatg gagctccagg acgcccagga tcagctggac
    15301 tacaagctac gacaggctga gagcgtgaag aattcttgga agcctgttgg ggagctgctt
    15361 gtagacgatt tgcaaaacca catagacaga gtgaaggcat ttcaggaaga gatagctccc
    15421 attcaggata acgtaaatca tgtcaaccag ctggcctcca catttagacc gtctgacatt
    15481 cagctttctc cagacaacct gagcagaatc gatgacctca acatgagatg gaggcttctg
    15541 cagatctcca ttgaggagca tctgagtcag ctgaccacag cctttaagga cttgggaccc
    15601 tctcagaatt tccttcatgc gtctgttgaa agccctctcg aacgctctat ttcacccaac
    15661 aatgtcccct actatatcaa tcaccagacc caaacaacat gttgggacca cccaaagatg
    15721 gcagaactct accagtcatt agcggatctc aacaacgtgc ggttctcggc atacaggacg
    15781 gcaatgaagc tcagacgaat gcagaaagcc ctctgtttgg atcttctgag catgcctgca
    15841 gcctgtgaag cctttgagca gcacaatctc aaacagaacg agcagttcat ggacatcgtg
    15901 caggtgatca actgtctgac cagcatctac gaccgtctgg agcagcagca cagcagcctg
    15961 gtcaacgtgc ctctctgtgt ggacatgtgt ctcaactggc tgctcaacgt ttacgatacg
    16021 ggacgagctg ggaagattcg taccctatcc ttcaaaacag gaataatctc tttgtgcaaa
    16081 gctcaccttg aagataagta cagattttta tttcgagagg tggccagtgc cacaggcttc
    16141 tgtgaccagc ggcgcctcgg cctcctcctg catgatgcca ttcagatccc caggcagctg
    16201 ggtgaagtgg cgtctttcgg agggagcaat attgagccca gtgtgcgcag ctgctttcag
    16261 ttcgccaata acaaaccgga gttagaggct tcagtcttcc tggactggat gcgtttagaa
    16321 cctcagtcga tggtttggct tcctgtcctt caccgtgtag cggccgctga gacagcaaag
    16381 caccaggcta agtgcaacat ttgtaaggaa tgtcctatta ttggcttcag gtaccgaagt
    16441 ttaaagcact ttaactatga tatctgccaa agctgcttct tttctggcag agtggccaaa
    16501 ggtcacaaga tgcagtaccc tatggttgaa tattgcacac cgacgacgtc aggagaggat
    16561 gtgagagact ttgccaaggt gttaaagaac aagttcagga caaagcgcta ttttgccaag
    16621 caccctcgca tgggttacct tcccgtccag accatccttg aaggagacaa catggagact
    16681 cctgttacac tgatcaactt ctggcctgtt gaccatccgc ctgcatcttc ccctcaactc
    16741 tcccatgacg acacccactc tcgcatcgag cattacgcta gcaggctagc tgaaatggag
    16801 aaccgaaatg gctcgtatgt gaatgacaat gtatcgccca atgaaagcat ggatgatgag
    16861 catctgctga tccagcacta ctgccagagt ctgaatcaag gctctcctct cagccagccg
    16921 cagagccccg ctcagatcct catctcaatg gagactgaag agaagggaga gctggagaga
    16981 gtgctcaatg atctggagca ggaaaacagg aagctgcaag cggagtatga tcgtctgaaa
    17041 aaggcacacg atcacaaggg tctgtcaccg ttgccttcac ctccacagat gcttccggtg
    17101 tcgcctcaga gtccacgcga cgcagaactg atcgcagaag ccaaactact gcggcaacac
    17161 aagggacgat tggaggcaag aatgcaaatc ctggaggatc acaacaagca gctggaatcg
    17221 cagcttacac gcctaagaca gttactcgag cagactgagt ccaaggtgaa tggcactgcc
    17281 ctgtcctcac cctccactgc ctctccgaga tctgacacca gcctggcctc actgcgtgtg
    17341 gccgcaagcc aaaccacaga gaccatgggt gatgatgagc tgtccagtcc cacccaggat
    17401 gcaagcactg gattagaaga cgtcatcgag cagctcaaca actccttccc tcacagccaa
    17461 ggaggcggac ggctaaatcc aatggtgagc aagggcgagg agctgttcac cggggtggtg
    17521 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
    17581 ggcgagggcg atgctaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
    17641 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
    17701 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
    17761 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
    17821 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
    17881 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
    17941 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
    18001 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
    18061 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
    18121 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
    18181 atggacgagc tgtacaagta acccagcttt cttgtacaaa gtgggggatc cagacatgat
    18241 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
    18301 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
    18361 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
    18421 ttccaacttt attatacata gttgataatt cactggccgt cgttttacgg taccatcgat
    18481 gatgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
    18541 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
    18601 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
    18661 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
    18721 gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
    18781 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
    18841 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
    18901 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
    18961 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
    19021 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
    19081 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
    19141 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
    19201 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
    19261 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
    19321 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
    19381 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    19441 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    19501 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    19561 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    19621 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    19681 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    19741 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    19801 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    19861 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    19921 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    19981 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    20041 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    20101 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    20161 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    20221 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    20281 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    20341 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    20401 ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa
    20461 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    20521 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    20581 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    20641 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    20701 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    20761 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    20821 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    20881 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    20941 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    21001 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    21061 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    21121 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    21181 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    21241 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    21301 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    21361 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    21421 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    21481 agaaaaataa acaaataggg gt
//
