LOCUS       sequence-341035-        8099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. Typhimurium stm2585 (sarA/steE) flanked with endogenous 
            regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, 
            Brennan RG, Ko DC
  TITLE     The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor 
            Signaling to Activate STAT3.
  JOURNAL   Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 
            10.1016/j.chom.2019.11.012. Epub 2019 Dec 31.
  PUBMED    31901521
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.11.012"; journalName: "Cell Host Microbe"; 
            date: "2020-01-8- 8"; volume: "27"; issue: "1"; pages: "129-139.e4"
COMMENT     Sequence Label: pWSK129-stm2585
FEATURES             Location/Qualifiers
     source          1..8099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(262..281)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             273..1088
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             273..1088
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(346..365)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2498..2515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2632..2653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2668..2673,2674..2691,2692..2698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2706..2722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2711..2733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2730..2746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2730..2746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2765..2785
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2767..2785
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4200..4219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4201..4219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4229..4246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4229..4245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4238..4260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4387..4815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4474..4493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4684..4705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5815..6037
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6085..7035
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc
       61 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg
      121 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc
      181 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
      241 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt
      301 gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      361 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
      421 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
      481 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
      541 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
      601 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
      661 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
      721 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
      781 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
      841 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
      901 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
      961 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1021 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1081 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     1141 gggacggcgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca
     1201 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc
     1261 cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc
     1321 gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctattc
     1381 tgaccttgcc atcacgactg tgctggtcat taaacgcgta ttcaggctga ccctgcgcgc
     1441 tgcgcagggc tttattgatt ccatttttac actgatgaat gttccgttgc gctgcccgga
     1501 ttacctgcat attaaagagc tgtatgtttc tcagtgcgat cgaaataagg gcacgggtaa
     1561 agcgataatg cgctttatag cccggcttgc gcttgaacag gaatgcctta gccttagctg
     1621 gaacgctgaa aaatccaacc ccggcgctaa ccgtttttat caggctctgg gaggcagaat
     1681 aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg
     1741 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     1801 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     1861 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac caggcgttta
     1921 agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg
     1981 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2041 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2101 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2161 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2221 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2281 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2341 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attgatctca
     2401 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2461 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2521 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2581 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2641 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2701 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2761 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg
     2821 ctctagacta catgccaact ttacttagta tcatattttc ttgctacata tctgtttggc
     2881 aagggtatga ataaaaaaca agattaattt aattgatatc gcaacagata agcacctgca
     2941 tttatcttta aactttttta tggccaacca gatttttcac catctgcgca cgtttattcg
     3001 ggcatgcgtt gaaaacctgc aaaaaaagaa aaatatcgat gcttattatt ttttctttaa
     3061 ttaaattttc gctcaacaaa cttaattatt aatccaatga cagtgaagtg tgaactatgc
     3121 tgagcataaa cgacatcaat agcaagggca agggcaaggg caagggcaag ggcaagggca
     3181 agggcaaggg caagggcaat ctgagtattt acaggtgaaa ttatgaaaaa tagtatattt
     3241 ttaccattgc ttctggtgct gtcggcaacg accttttcgg cattggtgat ggctgccagt
     3301 cattctcccc accagataat acaaaacatt tttccagtgg ctggccgcca gatatcctga
     3361 agcaactgaa ttggtctcag gtttgtggtt gttagtatac tgaatattac ttcaggatgg
     3421 catgttttat tgacaccagc ctgatttttc acaacgttat attacccgtt tacagataat
     3481 tcccagctaa taaatcagga agttccagat gtaacagaag ttatgaggtg tattaattga
     3541 taggtctaac aggcttgaat tacttttctt taaaaactac tgcatgtaaa agggtctcct
     3601 cttgttgtga tgagattcgt atatatttat atcttagtga tttatggatc atacttgtgg
     3661 ttttccttag gaggtaacat gtttacaatt aatagtacta acagggtggc gagcacaatc
     3721 gctccatatg catgtgtaag cgatgttaat ttagaggaca aggctacatt tttagatgaa
     3781 catacctcaa tacatgcgaa tgattcttct ttgcaatgct ttgtcttaaa tgatcagcat
     3841 gttccgcaga atacattagc tacagatgtt gaaggctaca atagaggatt acaggaacgt
     3901 ataagcctcg agtaccagcc cctggaaagc attgtttttc tactcggcac ccctgcagtt
     3961 ttagagacta aagagtcttt atcattacca gtttccccgg atgctttaac ccaaaaacta
     4021 ttaagcatta gtagcaatga tgaatgtaaa ttatcaggta gcaccagttg cactacccca
     4081 gccagtcata acccaccgtc cggttatatc gctcaataca ggcattctgc agaggttttc
     4141 ccggatgaat aaaacaaagc gttataaata gctgcgccga gggcccggta cccaattcgc
     4201 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     4261 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4321 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4381 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4441 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4501 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4561 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4621 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4681 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4741 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4801 aatttaacgc gaattttaac aaaattcgac cgatgccctt gagagccttc aacccagtca
     4861 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4921 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     4981 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     5041 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     5101 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     5161 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     5221 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     5281 cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg
     5341 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     5401 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     5461 gcggcacctc gctaacggat tcaccactcc gcagacccgc cataaaacgc cctgagaagc
     5521 ccgtgacggg cttttcttgt attatgggta gtttccttgc atgaatccat aaaaggcgcc
     5581 tgtagtgcca tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac
     5641 gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg
     5701 cccgagcttg cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga
     5761 gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta
     5821 tacacagggc tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt
     5881 ataaccactt gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt
     5941 ctagcagaat ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact
     6001 caaaggaaaa ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa
     6061 aatcacctag accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta
     6121 gcgattagtc gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca
     6181 ctactcaacc ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat
     6241 aaccaatacg ctcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa
     6301 gcaaccagag agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt
     6361 gagattttcc agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt
     6421 agtgaagaga tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa
     6481 catgttaagt cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa
     6541 ctaacacaaa agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc
     6601 atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa
     6661 ccaataagta aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc
     6721 cgcccgactg atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc
     6781 gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca
     6841 gattcctacc tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt
     6901 cagctcacca gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat
     6961 ggttcgttct catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa
     7021 tacggaagga tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac
     7081 aaacaaaagt agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccatatgcac
     7141 agatgaaaac ggtgtaaaaa agatagatac atcagagctt ttacgagttt ttggtgcatt
     7201 caaagctgtt caccatgaac agatcgacaa tgtaacagat gaacagcatg taacacctaa
     7261 tagaacaggt gaaaccagta aaacaaagca actagaacat gaaattgaac acctgagaca
     7321 acttgttaca gctcaacagt cacacataga cagcctgaaa caggcgatgc tgcttatcga
     7381 atcaaagctg ccgacaacac gggagccagt gacgcctccc gtggggaaaa aatcatggca
     7441 attctggaag aaatagcgct ttcagccggc aaaccggctg aagccggatc tgcgattctg
     7501 ataacaaact agcaacacca gaacagcccg tttgcgggca gcaaaacccg tacttttgga
     7561 cgttccggcg gttttttgtg gcgagtggtg ttcgggcggt gcgcgcaaga tccattatgt
     7621 taaacgggcg agtttacatc tcaaaaccgc ccgcttaaca ccatcagaaa tcctcagcgc
     7681 gattttaagc accaaccccc ccccgtaaca cccaaatcca tactgaaagt ggctttgttg
     7741 aataaatcag atttcgggta agtctccccc gtagcgggtt gtgttttcag gcaatacgca
     7801 cgctttcagg catacctgct ttcgtcattt tgttcagcgc tcgtaccagg gccatagcct
     7861 ccgcaacctg accatcgtag tcacgcagcg tcagtgaacc cccgaacagc tgtaatccgg
     7921 gcagcgcaac ggaacattca tcagtgtaaa aatggaatca ataaagccct gcgcagcgcg
     7981 cagggtcagc ctgaatacgc gtttaatgac cagcacagtc gtgatggcaa ggtcagaata
     8041 gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgccc
//
