LOCUS       sequence-341037-       14110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genome editing of C. albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Evans BA, Bernstein DA
  TITLE     SpRY Cas9 Can Utilize a Variety of Protospacer Adjacent Motif Site 
            Sequences To Edit the Candida albicans Genome.
  JOURNAL   mSphere. 2021 May 19;6(3). pii: 6/3/e00303-21. doi: 
            10.1128/mSphere.00303-21.
  PUBMED    34011687
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2021-05-19"; volume: "6(3). pii: 6/3/e00303-21. doi"; pages: 
            " 10.1128/mSphere.00303-21"
COMMENT     Sequence Label: pV1093:SpRY
FEATURES             Location/Qualifiers
     source          1..14110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(15..470)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(152..173)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     364..383
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     597..619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     611..628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     612..628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6358..6378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6382..6402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6406..6426
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6427..6492
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      6563..6810
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6579..6597)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     misc_RNA        9727..9802
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(11934..11954)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11934..11952)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11973..11989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11973..11989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11986..12008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11997..12013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12021..12027,12028..12045,12046..12051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12066..12087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12204..12221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12375..12963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12455..12474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13134..13925,13926..13994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13757..13776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13995..14099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
       61 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      121 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      181 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      241 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      301 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      361 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      421 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
      481 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      541 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      601 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
      661 attgaggcct gcatgcggta cctgccacta ctaccactgg gagtttcgtt cttctcgata
      721 ctattagctt tacttcctgc actagcagtg gttggatcaa cagaatcttc ataatcatca
      781 aaatcgtctt ttgaagaccc cccgtttgat gtatggccct gtcttttcat caaacttttt
      841 atatagttga ctgaactgag gctaaatatg tgatcatctt cactatagac aatctttctc
      901 ttatttgcac caccgccacc accagtcttt gagaaattct caaaaccttt tacgatatta
      961 ccaagcgggc tctcttcgaa ataatctatc tctttttgat atatcgaatc ctctagcgtg
     1021 gttagctttc tagttagttc ttgcttctta agaatttgct ggattagttt atttttcaat
     1081 tcaacgtatt tctcagagtc atctttagat tttgatgaag atgtgcgttc attcgctata
     1141 tccttcttgg tcgtgtcttt tcgatcctcc ttggctggca ctgaactcgt cttttttggc
     1201 gttgctgttc cagacagact tatctcatta gatttggaac ttgtgggttt aacatcattt
     1261 gcatctttag tagacatgat tgtgtaatac cgtgattgtt tgttttgaaa ggtctgtcat
     1321 atttctatca atttcaaaac aaaatgttca tcagaaaaaa gccaaaaatg tctcttctag
     1381 cttcttagtg gtgtcgcaaa atacacaatg tcgctcaaca atccacattc ccggcgcata
     1441 gctcaaatca catgactaca gctaacaatt acacaaaaaa attctctttt cgatgtagca
     1501 actatcttca actaaaacat tttctccttc ggcccatgat tgtcctccgg gtcgacagca
     1561 agccgttaca attgagatgg aaagcgacct accttcactc gataaggtgc ttaattgtac
     1621 ttcatataaa tctggcccgg atctaaacaa atgagttcca ttaagccgtg ggttctcaat
     1681 tagggttttt gtttttgatt tagaaaaaag agatcaagat ttgtttacag gtgatgcctt
     1741 tttttagaac ttatgcgttg caaaagttga ctaacgattt ctataaggtg atccacacta
     1801 attatacaaa cgtacaaaca gacatacttt tcctgcgttc acctgatgtt ggccagattt
     1861 ctctcttcat tgcatagaac ataaccacac tagggcaaca gaaaaaaaaa aaaaaagtgc
     1921 atcgggaagt tgtgttccat tcattatatg tctactactg catatgagta gcccacccac
     1981 caccaccata gtaagttttt gtgtatgcgc gccgtcaggt tatttcattt ctgaattttt
     2041 caaccacctt actcccttta ttgttgattg acaattttgc tcacagtaag atcttttaga
     2101 ctccaattaa tataaaataa gtctgatttt ccaattcctg ttttttcttt ttttttctgt
     2161 ttctatttct ttccttttct cccttttttt taattcttca ttcaatcatc aattgataat
     2221 tcaggaatat tacaacccgg gatggataaa aagtatagta ttggtttaga tattggtact
     2281 aactctgtgg gttgggcagt tatcaccgac gaatataaag ttccatcaaa gaaatttaag
     2341 gtgttaggta acactgacag acactcaata aaaaagaatc ttatcggtgc tcttttgttc
     2401 gactccggtg aaactgccga gagaacacgt ttaaaaagaa cagcaagaag aagatatacc
     2461 cgtagaaaaa atagaatatg ttatttacaa gaaatctttt ctaatgaaat ggctaaagtt
     2521 gatgattcct ttttccatag attggaagag tcatttttgg ttgaagaaga caaaaagcat
     2581 gagagacatc caatctttgg gaatatagtt gatgaagtgg cttaccatga aaaatatcct
     2641 accatttatc atttaagaaa gaaattggta gattcaactg ataaagctga ccttagatta
     2701 atctatttag cacttgccca tatgattaaa tttagaggtc attttttgat tgaaggtgat
     2761 ttgaacccag ataattctga cgtggataaa ttatttattc aattagtcca aacctacaac
     2821 caattatttg aggaaaatcc aattaatgct agtggtgtcg atgccaaagc tatattatca
     2881 gccagattat caaaatctag acgtttggaa aatttgattg cccaattgcc aggagaaaaa
     2941 aagaatggat tatttggaaa cttgatcgca ttatcattgg gtttgacacc aaattttaaa
     3001 tctaattttg atttagctga agatgctaaa ttacaattat caaaagacac ctatgacgac
     3061 gatttggaca atttacttgc tcaaattggt gatcaatatg cagatttgtt cttagctgct
     3121 aaaaacttat ctgatgctat tttgttgtct gatattttga gagtgaacac agaaataacc
     3181 aaagctccat tatcagcatc tatgatcaaa cgttatgatg aacaccatca ggatttgact
     3241 ttattgaaag ctttggtgag acaacaattg ccagagaagt ataaagaaat ctttttcgat
     3301 caatctaaaa acgggtatgc aggttatatt gatgggggtg cctcccaaga ggaattttac
     3361 aaatttataa aacctatttt agaaaagatg gatgggactg aggaactttt ggtcaaattg
     3421 aacagagaag atttgttacg taaacagaga acttttgata atggtagtat acctcaccaa
     3481 attcatttgg gtgagttgca tgcaatttta agaagacaag aagattttta tccattttta
     3541 aaagataata gagaaaaaat cgagaaaatt ttaaccttta gaattccata ctatgttggg
     3601 cctttggcta gaggtaattc aagatttgcc tggatgacac gtaaatcaga agaaactatt
     3661 accccttgga attttgaaga ggttgttgat aaaggagcat cagcacagag ttttattgaa
     3721 agaatgacca atttcgataa aaacttacca aatgaaaaag ttttaccaaa acattccttg
     3781 ttatacgaat attttactgt ttacaatgaa cttacaaagg ttaaatatgt tactgaaggt
     3841 atgcgtaagc cagccttttt atctggagaa cagaaaaagg caatagttga tttattgttt
     3901 aaaacaaata gaaaagttac tgttaaacaa ttaaaagaag attactttaa gaaaattgaa
     3961 tgttttgatt cagttgaaat cagtggtgtt gaagacagat ttaatgctag tttaggaact
     4021 taccatgatt tacttaaaat tatcaaagat aaagatttct tggataacga agaaaatgaa
     4081 gacattttag aagacattgt tttaacctta actttattcg aagatagaga gatgattgaa
     4141 gaacgtttga agacttatgc acatttgttt gacgataaag tgatgaaaca gttgaaaaga
     4201 agacgttata ctggatgggg tagattgtct cgtaaattga tcaatggaat tagagataaa
     4261 caaagtggta aaactatctt ggactttttg aaatctgacg gatttgctaa tagaaatttc
     4321 atgcaattga tccacgacga tagtttgaca tttaaagaag acatccaaaa ggcccaagtg
     4381 agtgggcaag gtgattcatt acatgaacat attgcaaatt tagccggatc tcctgctatt
     4441 aagaaaggga tattacaaac tgttaaagtt gtggatgaat tagtgaaagt aatgggaaga
     4501 cataaacctg aaaacattgt cattgagatg gcaagagaaa atcaaactac acaaaaagga
     4561 cagaaaaata gtagagaacg tatgaaaaga atagaagagg gtattaaaga attgggtagt
     4621 caaatattga aagaacaccc agtggaaaat acccagttgc aaaatgaaaa attatatctt
     4681 tactaccttc aaaatggacg tgatatgtat gttgatcagg aattagatat aaatagactt
     4741 tcagattatg atgtagatca tatagttcca caatctttct tgaaagatga ttccatagac
     4801 aataaagtat taactagaag tgataaaaat agaggtaaaa gtgataatgt cccaagtgag
     4861 gaagtcgtca aaaagatgaa aaattactgg cgtcaacttt tgaatgctaa attaattact
     4921 caaagaaaat ttgataattt gactaaagca gaaagaggtg ggctttctga attagataaa
     4981 gccgggttca ttaaaagaca attggtcgaa actagacaaa ttactaaaca tgttgcccaa
     5041 attttagatt cccgtatgaa cactaagtat gacgaaaatg ataagttaat acgtgaggtt
     5101 aaagtcatta ctttaaaatc aaaacttgtc tctgatttca gaaaggattt ccaattctat
     5161 aaagttagag aaattaataa ttatcatcat gctcatgatg catatttgaa tgctgtagtt
     5221 ggaactgctt taatcaagaa ataccctaaa ttagaatctg aatttgtata tggtgattac
     5281 aaagtctatg atgttagaaa gatgattgct aaatcagaac aagaaattgg taaagctaca
     5341 gctaaatact tcttttactc taacattatg aatttcttta aaacagaaat tactttggca
     5401 aacggtgaaa ttagaaaaag acctcttatt gaaacaaatg gtgagactgg agagatagtt
     5461 tgggacaaag ggcgtgattt cgctactgtt agaaaagttt tatcaatgcc acaagttaac
     5521 attgtaaaga aaacagaggt tcaaactggt ggtttctcaa aagaaagtat tagacctaaa
     5581 agaaatagtg ataaattgat tgccagaaaa aaggattggg atccaaagaa atatggtggt
     5641 ttcttatggc caaccgtagc ctattctgtt ttggttgtgg caaaggttga aaagggtaaa
     5701 agtaaaaagc ttaaatcagt aaaagaactt ttgggtatta caataatgga aagaagttcc
     5761 tttgaaaaga accctattga ttttttggaa gctaaaggtt ataaggaagt aaagaaggac
     5821 ttaataatca aattgcctaa atattcttta tttgaattag aaaatgggag aaaaagaatg
     5881 ttggcttctg ctaaacaatt gcaaaagggt aatgaattag cattgccttc caaatatgtt
     5941 aacttcttgt atttagcttc acactatgaa aagttgaaag ggtcaccaga agataacgag
     6001 caaaaacaat tatttgttga acaacacaaa cactacttag atgagattat agaacaaatt
     6061 agtgaattca gtaaaagagt gatattagct gatgcaaatt tagataaagt tttgtcagcc
     6121 tataacaaac atagagataa gccaattaga gaacaagcag aaaacattat tcacttattt
     6181 acccttacca gattaggagc acctagagct ttcaagtatt ttgatacaac aattgatcca
     6241 aaacaatata gatcaacaaa agaagtctta gacgccacct taattcatca atcaatcact
     6301 ggattgtatg agacaagaat tgatttgtct caattgggtg gtgatgaagg ggctgatcct
     6361 aagaagaaaa gaaaagttga tccaaagaaa aagcgtaagg tggatcctaa gaaaaagaga
     6421 aaggttgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     6481 gacgatgaca agtgataatg ataatgactg cagagatcca tcgacctgcc gccaagctaa
     6541 ttccgggcga atttctgtcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct
     6601 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     6661 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     6721 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     6781 gttttgggac gctcgaaggc tttaatttgc ggccgggccc cccctaactc aagtacaaca
     6841 gatctggacc acctttgatt gtaaatagta ataattacca cccttatcta attatttatt
     6901 taacttattt atttatttat tatacatata tacaaaccta ataaagtgaa aatctccccc
     6961 ttcacacttc acatatgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc
     7021 gctgtttcgt tcgagacttg aggtctagtt ttatacgtga agaggtcaat gccgccgaga
     7081 gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat
     7141 cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc
     7201 gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg
     7261 ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag
     7321 caagcagagt cttcatcaga gtcatcatct gagatgtaat ccttccggta ggggctcaca
     7381 cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca
     7441 atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaattttc
     7501 atatgagaac cgttatcgat aactaaagca gcaacttctt ctataaaaat gggttagtat
     7561 gacagtcatt taaataagga atttttcagt tggcttggtt tcaattcaat gttcgttttt
     7621 ttttttcttg ctgtgtttgt gtttgtgttg tttatagttg tgtgcactga tcgtcgaaaa
     7681 aaaaaattca tagtgagccg ggaaatctgt atagcccaga taacaacaca agtccaaact
     7741 agaaactcgt caaacaccaa aagcaatgtt gaatcaattg ccttgcacaa gtacacgtag
     7801 gaaaacataa aacattgcaa ttttgaatat tgagcctttt gtcgtaacat tgattgatag
     7861 gattactcac cgaatggttt tgaaaccact gccgacagat caatcaatca atcaaaaaac
     7921 gtgaactttg aaaaagggga agaacagata cattgaagtt agccatttcc actgatcgtc
     7981 acaacatatc tgataaatta ctttcaaaat tataagctga tgtgtgtgta ttattaatgt
     8041 gacagtaaca tcccaaacga gaaatattat ctcgacaaca aaaaagtttg atctgaattg
     8101 aaaatgaagt tttcccaccc tacccatttg tcatattgaa accaatcaac tgattaatca
     8161 atcaattaga attgaagcta aactaaaaca taccaccgtc cattttgaat gattatattt
     8221 ttttaatatt aatatcgaga taatgtttct aagaaagaaa gaaaaccagg agtgaaaatt
     8281 agaaaaggaa aggaaagaaa aaaagaaaaa tctgaaaata tataaaaaaa aattgtttcg
     8341 ttggcaataa atcttggtga gaacagcgac cgaaagcaaa taagaacaaa atatgagtgt
     8401 attacgttga acaactaatt aacgtgtgtg tatggatctt tttttctttt ttctctttaa
     8461 ccgactataa acaacaaaca tttttgggca gtgcacacac tacttaatat acacagcata
     8521 aattacacga ttagaaacaa attagcttat taaaataacc taatcaaacc gaatatttta
     8581 tggtattatg agtaaactat ataatataaa tagcacacac ccacaacaac aacaaaggaa
     8641 aactaaaagg ttttttcttt ttgaaaagat cgttttcttt attattctct agttttgacg
     8701 gcggccgcag tgattagact tagtccgttc aaatcaagca caactctgtt cattgtttca
     8761 acaagaatta attcaaaaac aggttcggtg cataatttgc aaaaaaatat tgcagcttct
     8821 gtggctcgaa cacagtacct ccagatttca ggtttgaaat acttcagtct gacgctctcc
     8881 cagatgagct aaagctgcaa taagaaaacc cacgccggga ttcgaacccg gaatcctttg
     8941 attagaagtc aaaagcgata accatttcgc cacgcaggcc tacttgatgg gtttgtaaat
     9001 ggtctacttt ttcagaccta acagaaattt taatgaaagt catattctta tacaataaaa
     9061 ctgtgtcata aaagcagata ttcgactttc gtagattata taggacccaa gaactaaaat
     9121 ttaatgccat attatgcatt tttaatctgt aaaagtgttg tttccaacct atcacaagta
     9181 cgttcttgta acttgtgttt gtagggttgc aaatgaatca taacaacatc tcaacagaac
     9241 atgtatagca aagcttagta taaaatcagt gttttgagag gcaatccaag aatgtttaca
     9301 tcaaagtttc aataaatatc gaccgaaact gaaaatcttt ttaggttatt gttcactttt
     9361 ttgtaaatat ttaaacattt tttggaccta aaaaaataca agcaccaatt acgtaccaag
     9421 aagcatctaa tcaactccca gatcaccact atacatttaa aagtcattgg tcaataacta
     9481 tactcgagta ttgcctcatc aaagaaacaa tcaaatatta tagatactca ctccatcacg
     9541 tgataatttc actggtatgg aaaagtggaa aattttataa aaaaaaattt gatgcctttg
     9601 gcatagctga aacttcggcc caataggatt ggagaatatg ttttcgcagc gttcttacaa
     9661 ttaaattgtg gtggaagttc gagacttgcg taaactattt ttaatttgga gacggaattc
     9721 cgtctcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     9781 aaaagtggca ccgagtcggt gctttttttc tcgagttttt ttatcgagtg tttaaggata
     9841 atgataactg aagagaagaa ttagttttgc cgccaccgcg ggtttgcctc tgattaaata
     9901 aaaaaaagct ggtgcttttt tttcttttat aggaacatct tgaatatatg aactaattaa
     9961 atgataattt tttacccatc tttactctta atcactgagc tgcagtcaaa gaaaaaggga
    10021 tacagcacct ggtgaagaga tgaacggaga ctaacttaga cgcgttgatt cttttcaatt
    10081 gcacatttta ttaatcgatg ctaacgtcta tttacatata ttctttagag atattatcta
    10141 gggcttcaaa taatctctgg acagcaataa aagtctcttc aaaagtattg tataacggca
    10201 atggggctaa tctgattaca tctggtcttc tttcgtcaca gattatagca tgatcatgca
    10261 agtacgcatt aactcgttcc atgacgttct tgcccttttc atcgaaatgc ggttgaaaca
    10321 taatggacaa ttgacatcct ctttcagctg gattcaaagg agttaaaatt ttaaacccaa
    10381 atttggagtt tgatgtactg gattgtggta tgtaatactt ggaattcgtc aatagatcct
    10441 gtaaaaattg agtcaaagca acactttttt cacgaagttt agatactcca cccactttag
    10501 catacacttc caatgacgac ttcacagcaa caacatcaag aacagaagga tttgactgtc
    10561 tgtaagaaag agccgagttt attggatcaa actcttctaa cattttgaat cgttcttggg
    10621 agttattgcc ccaccaacca gctagtctag gaacgaaact gctcttcttg ttctctatag
    10681 tgtatttttc atgcacaaaa attccaccta tggctccagg tcccgagttt aaatatttgt
    10741 aggaacacca agcagcaaaa tctactcccc aatcatgtaa atttaatgga acattcccaa
    10801 ctgcatgggc aagatcccac ccaactttaa tttgttggct cttttcctta gcgtatttag
    10861 ttatttcctc tatcttgaaa aattgaccag tgtagtattg gataccagga aaacacacta
    10921 gagccaattc atccaggttc tcatctatag ccttgattat tctttctgtt ttaatataag
    10981 tttcaccagg ttgaacttcc aattgaatca aatgtttctc gtcgtatccg aacaatttaa
    11041 caatgttcaa aaatgcatag tagtcagaag gaaatgcttg tttttcaaat aaaattttgg
    11101 ttcttttccc ctcaggtttg taaaaatgga tcaacaatgc attcaagttt gctgttaaag
    11161 aacccataac tgcaacttcg ttttcctttg caccaacaat gggggctatt aatggtaata
    11221 agggtaaatc gatgtctacc cacggtgtta acagtttgtc aggatgattg aaatgagact
    11281 caacccctcg ttcaacccat gcattcaatt catcattgat agctttcttt gtattcttag
    11341 gcatcaaccc aagagagttt ccacataaat aaatagactc agttgatgac tcatatttat
    11401 tatttttgat acctaatgat ccaaaagttg gtatggcaaa ctcattttta aaagttggga
    11461 actttttgtc caatttcttt gcctcggcta atgacatctg ataataaaat ggggttggag
    11521 tagttggtgg tataaccgga gagatagaat tgaagaaaaa aatcggaaac aacaaaaaaa
    11581 gttgataccc agtattatgt gggagataat tgcgaatggt ggaaaaaaaa aagacgcctt
    11641 tgggcctcaa caacaattct aacagcttga gagctttaca atcgagaaac tatgattcat
    11701 tccgttttaa tatgtatgta cttagtaaac tcgtgaattt tatttgtggt ctactttagt
    11761 actaacataa tcattggata gtcaataatg atggtcttcc gagactaatg aaattctata
    11821 ccaaagtcga tattccaaca cagaaattgc tcttgcaaca agtgcacctg ttgatatcta
    11881 ctagatcagc acaagagtgt tcggagctcg catgcaggcc tcagcttttg ttccctttag
    11941 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    12001 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
    12061 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    12121 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    12181 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    12241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    12301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    12361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    12421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    12481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    12541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    12601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    12661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    12721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    12781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    12841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    12901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    12961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    13021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    13081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    13141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    13201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    13261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    13321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    13381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    13441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    13501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    13561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    13621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    13681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    13741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    13801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    13861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    13921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    13981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    14041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    14101 acatttcccc
//
