LOCUS       sequence-341038-       14985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genome editing of C. albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Evans BA, Bernstein DA
  TITLE     SpRY Cas9 Can Utilize a Variety of Protospacer Adjacent Motif Site 
            Sequences To Edit the Candida albicans Genome.
  JOURNAL   mSphere. 2021 May 19;6(3). pii: 6/3/e00303-21. doi: 
            10.1128/mSphere.00303-21.
  PUBMED    34011687
REFERENCE   2  (bases 1 to 14985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2021-05-19"; volume: "6(3). pii: 6/3/e00303-21. doi"; pages: 
            " 10.1128/mSphere.00303-21"
COMMENT     Sequence Label: pV1393:SpRY
FEATURES             Location/Qualifiers
     source          1..14985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4257..4277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4281..4301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4305..4325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4326..4391
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4462..4709
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4478..4496)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4730..4746)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4730..4746)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5825..7096
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     terminator      complement(7523..7720)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(8321..8664)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     misc_RNA        9733..9808
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9888..9921)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(10288..10306)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10327..10343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10327..10343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10340..10362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10351..10367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10375..10381,10382..10399,10400..10405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10420..10441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10558..10575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10729..11317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10809..10828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11488..12279,12280..12348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12111..12130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12349..12453)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12479..12934)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12616..12637)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12828..12847
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13061..13083
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13075..13092
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13076..13092
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13102..13121
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13102..13120
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(13573..13606)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tccaattaat ataaaataag tctgattttc caattcctgt tttttctttt tttttctgtt
       61 tctatttctt tccttttctc cctttttttt aattcttcat tcaatcatca attgataatt
      121 caggaatatt acaacccggg atggataaaa agtatagtat tggtttagat attggtacta
      181 actctgtggg ttgggcagtt atcaccgacg aatataaagt tccatcaaag aaatttaagg
      241 tgttaggtaa cactgacaga cactcaataa aaaagaatct tatcggtgct cttttgttcg
      301 actccggtga aactgccgag agaacacgtt taaaaagaac agcaagaaga agatataccc
      361 gtagaaaaaa tagaatatgt tatttacaag aaatcttttc taatgaaatg gctaaagttg
      421 atgattcctt tttccataga ttggaagagt catttttggt tgaagaagac aaaaagcatg
      481 agagacatcc aatctttggg aatatagttg atgaagtggc ttaccatgaa aaatatccta
      541 ccatttatca tttaagaaag aaattggtag attcaactga taaagctgac cttagattaa
      601 tctatttagc acttgcccat atgattaaat ttagaggtca ttttttgatt gaaggtgatt
      661 tgaacccaga taattctgac gtggataaat tatttattca attagtccaa acctacaacc
      721 aattatttga ggaaaatcca attaatgcta gtggtgtcga tgccaaagct atattatcag
      781 ccagattatc aaaatctaga cgtttggaaa atttgattgc ccaattgcca ggagaaaaaa
      841 agaatggatt atttggaaac ttgatcgcat tatcattggg tttgacacca aattttaaat
      901 ctaattttga tttagctgaa gatgctaaat tacaattatc aaaagacacc tatgacgacg
      961 atttggacaa tttacttgct caaattggtg atcaatatgc agatttgttc ttagctgcta
     1021 aaaacttatc tgatgctatt ttgttgtctg atattttgag agtgaacaca gaaataacca
     1081 aagctccatt atcagcatct atgatcaaac gttatgatga acaccatcag gatttgactt
     1141 tattgaaagc tttggtgaga caacaattgc cagagaagta taaagaaatc tttttcgatc
     1201 aatctaaaaa cgggtatgca ggttatattg atgggggtgc ctcccaagag gaattttaca
     1261 aatttataaa acctatttta gaaaagatgg atgggactga ggaacttttg gtcaaattga
     1321 acagagaaga tttgttacgt aaacagagaa cttttgataa tggtagtata cctcaccaaa
     1381 ttcatttggg tgagttgcat gcaattttaa gaagacaaga agatttttat ccatttttaa
     1441 aagataatag agaaaaaatc gagaaaattt taacctttag aattccatac tatgttgggc
     1501 ctttggctag aggtaattca agatttgcct ggatgacacg taaatcagaa gaaactatta
     1561 ccccttggaa ttttgaagag gttgttgata aaggagcatc agcacagagt tttattgaaa
     1621 gaatgaccaa tttcgataaa aacttaccaa atgaaaaagt tttaccaaaa cattccttgt
     1681 tatacgaata ttttactgtt tacaatgaac ttacaaaggt taaatatgtt actgaaggta
     1741 tgcgtaagcc agccttttta tctggagaac agaaaaaggc aatagttgat ttattgttta
     1801 aaacaaatag aaaagttact gttaaacaat taaaagaaga ttactttaag aaaattgaat
     1861 gttttgattc agttgaaatc agtggtgttg aagacagatt taatgctagt ttaggaactt
     1921 accatgattt acttaaaatt atcaaagata aagatttctt ggataacgaa gaaaatgaag
     1981 acattttaga agacattgtt ttaaccttaa ctttattcga agatagagag atgattgaag
     2041 aacgtttgaa gacttatgca catttgtttg acgataaagt gatgaaacag ttgaaaagaa
     2101 gacgttatac tggatggggt agattgtctc gtaaattgat caatggaatt agagataaac
     2161 aaagtggtaa aactatcttg gactttttga aatctgacgg atttgctaat agaaatttca
     2221 tgcaattgat ccacgacgat agtttgacat ttaaagaaga catccaaaag gcccaagtga
     2281 gtgggcaagg tgattcatta catgaacata ttgcaaattt agccggatct cctgctatta
     2341 agaaagggat attacaaact gttaaagttg tggatgaatt agtgaaagta atgggaagac
     2401 ataaacctga aaacattgtc attgagatgg caagagaaaa tcaaactaca caaaaaggac
     2461 agaaaaatag tagagaacgt atgaaaagaa tagaagaggg tattaaagaa ttgggtagtc
     2521 aaatattgaa agaacaccca gtggaaaata cccagttgca aaatgaaaaa ttatatcttt
     2581 actaccttca aaatggacgt gatatgtatg ttgatcagga attagatata aatagacttt
     2641 cagattatga tgtagatcat atagttccac aatctttctt gaaagatgat tccatagaca
     2701 ataaagtatt aactagaagt gataaaaata gaggtaaaag tgataatgtc ccaagtgagg
     2761 aagtcgtcaa aaagatgaaa aattactggc gtcaactttt gaatgctaaa ttaattactc
     2821 aaagaaaatt tgataatttg actaaagcag aaagaggtgg gctttctgaa ttagataaag
     2881 ccgggttcat taaaagacaa ttggtcgaaa ctagacaaat tactaaacat gttgcccaaa
     2941 ttttagattc ccgtatgaac actaagtatg acgaaaatga taagttaata cgtgaggtta
     3001 aagtcattac tttaaaatca aaacttgtct ctgatttcag aaaggatttc caattctata
     3061 aagttagaga aattaataat tatcatcatg ctcatgatgc atatttgaat gctgtagttg
     3121 gaactgcttt aatcaagaaa taccctaaat tagaatctga atttgtatat ggtgattaca
     3181 aagtctatga tgttagaaag atgattgcta aatcagaaca agaaattggt aaagctacag
     3241 ctaaatactt cttttactct aacattatga atttctttaa aacagaaatt actttggcaa
     3301 acggtgaaat tagaaaaaga cctcttattg aaacaaatgg tgagactgga gagatagttt
     3361 gggacaaagg gcgtgatttc gctactgtta gaaaagtttt atcaatgcca caagttaaca
     3421 ttgtaaagaa aacagaggtt caaactggtg gtttctcaaa agaaagtatt agacctaaaa
     3481 gaaatagtga taaattgatt gccagaaaaa aggattggga tccaaagaaa tatggtggtt
     3541 tcttatggcc aaccgtagcc tattctgttt tggttgtggc aaaggttgaa aagggtaaaa
     3601 gtaaaaagct taaatcagta aaagaacttt tgggtattac aataatggaa agaagttcct
     3661 ttgaaaagaa ccctattgat tttttggaag ctaaaggtta taaggaagta aagaaggact
     3721 taataatcaa attgcctaaa tattctttat ttgaattaga aaatgggaga aaaagaatgt
     3781 tggcttctgc taaacaattg caaaagggta atgaattagc attgccttcc aaatatgtta
     3841 acttcttgta tttagcttca cactatgaaa agttgaaagg gtcaccagaa gataacgagc
     3901 aaaaacaatt atttgttgaa caacacaaac actacttaga tgagattata gaacaaatta
     3961 gtgaattcag taaaagagtg atattagctg atgcaaattt agataaagtt ttgtcagcct
     4021 ataacaaaca tagagataag ccaattagag aacaagcaga aaacattatt cacttattta
     4081 cccttaccag attaggagca cctagagctt tcaagtattt tgatacaaca attgatccaa
     4141 aacaatatag atcaacaaaa gaagtcttag acgccacctt aattcatcaa tcaatcactg
     4201 gattgtatga gacaagaatt gatttgtctc aattgggtgg tgatgaaggg gctgatccta
     4261 agaagaaaag aaaagttgat ccaaagaaaa agcgtaaggt ggatcctaag aaaaagagaa
     4321 aggttgacta caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg
     4381 acgatgacaa gtgataatga taatgactgc agagatccat cgacctgccg ccaagctaat
     4441 tccgggcgaa tttctgtcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     4501 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     4561 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     4621 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     4681 ttttgggacg ctcgaaggct ttaatttgcg gccgggcccc ccctcgaggg atccactagt
     4741 tctagatttt tgcaagcatt taaatattgc caagtaaaaa cttcaaattt tctttcccct
     4801 tggaactttg actttatttt tttgacagat tattttgaca cacacacacc aaatgtgtta
     4861 ccccttaaaa caaaaaaaca cttttttaca atttcttggt atccagaatc attctaagca
     4921 tcattcaatt ataatttcaa tccaaaaaag tagttttagt ttgacttgaa acgtcaacaa
     4981 acacaaattt caaatcataa cctctcctgt tgcctgtcaa caacacacca taaggagaag
     5041 gaataggagg aggaggagat agaaacttgc acggcaccac aaaacacaaa attgatttca
     5101 accaatacgg tgacaacaac aatagatttc cgatagaaat aatgattatc ggaataagct
     5161 agctttgctt tgctttgctt tgctttttga cttgctctaa tttttcgaaa ataataatgg
     5221 agaaaagttc aaggtgttta atgcatcaac taaaacagaa aataatacat tagactaaac
     5281 ttttaatctt tctagtacca ataattcacg cgtgcgtttt aatcccaatc atgaaatgaa
     5341 gaagttattt ccctttttct ttcatcaaaa aagaactaaa ttatttttta aattttagta
     5401 aacaaaacct ggaaatcggg gaaaccgggg gaggggggca gaaggtgaaa cgggtaatat
     5461 tgataaattt aatctataat tgataaagtt aaatttaaat tgatttgaat tgatttgaat
     5521 tgaatgaaat gcatttgaat aaacggcatc aaactaaaaa aatatagatc acattcatag
     5581 taaaacgata acaaagaaca ccacaattta tagcaatgat aataaacatc taaaaagaaa
     5641 agggtacgag aaggagaatg aaaaaaaaca ataagctagt tcttaatctg ttcagatatc
     5701 taatttcaaa aaaaagaata gtataaaagg atagttgatt cctcttggtt gttgaaaatt
     5761 tgaatgatat caatcaatta atcaatcaaa taacaacaac ccactagaca tcaccattgt
     5821 cgacatgcca caatttgata tattatgtaa aacaccacct aaggtgcttg ttcgtcagtt
     5881 tgtggaaagg tttgaaagac cttcaggtga gaaaatagca ttatgtgctg ctgaactaac
     5941 ctatttatgt tggatgatta cacataacgg aacagcaatc aagagagcca cattcatgag
     6001 ctataatact atcataagca attcgttgag tttcgatatt gtcaataaat cactccagtt
     6061 taaatacaag acgcaaaaag caacaatttt ggaagcctca ttaaagaaat tgattcctgc
     6121 ttgggaattt acaattattc cttactatgg acaaaaacat caatctgata tcactgatat
     6181 tgtaagtagt ttgcaattac agttcgaatc atcggaagaa gcagataagg ggaatagcca
     6241 cagtaaaaaa atgcttaaag cacttctaag tgagggtgaa agcatctggg agatcactga
     6301 gaaaatacta aattcgtttg agtatacttc gagatttaca aaaacaaaaa ctttatacca
     6361 attcctcttc ctagctactt tcatcaactg tggaagattc agcgatatta agaacgttga
     6421 tccgaaatca tttaaattag tccaaaataa gtatttggga gtaataatcc agtgtttagt
     6481 gacagagaca aagacaagcg ttagtaggca catatatttc tttagcgcaa ggggtaggat
     6541 cgatccactt gtatatttgg atgaattttt gaggaattct gaaccagtcc taaaacgagt
     6601 aaataggacc ggcaattctt caagcaataa acaggaatac caattattaa aagataactt
     6661 agtcagatcg tacaataaag ctttgaagaa aaatgcgcct tattcaatct ttgctataaa
     6721 aaatggccca aaatctcaca ttggaagaca tttgatgacc tcatttcttt caatgaaggg
     6781 cctaacggag ttgactaatg ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc
     6841 caggacaacg tatactcatc agataacagc aatacctgat cactacttcg cactagtttc
     6901 tcggtactat gcatatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa
     6961 tccaattgag gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg
     7021 ataccccgca tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat
     7081 aaatagacgc atataagagt gaaattctgg aaatctggaa atctggtttt gtattcttgt
     7141 tattcttctt tttgttatta catatataac ttgttacttt tttaaaaaaa tctttgtata
     7201 ttttataaat atataaaact aaatttaaga aaaagagaaa aatgttttat ttgagagatt
     7261 gaaattttac ttgaatttag cttagctttt ataaagtatt attatgtaaa aaaacaaaac
     7321 aaatatacat caaaaagtta agactataaa atagccaccc aaggcatttc tatatcttgt
     7381 tgttgttgtt ttcatcttct gtatcagagg aacttatttt attattttcg tcacgggtat
     7441 tttctcttgt ttgatgattc atcccattca ttccatcata aaatgtcgac actggatggc
     7501 ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg
     7561 atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa
     7621 tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa
     7681 atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgactcga gttattatgg
     7741 acatggcata gacatataca aagcttgttc accatcggaa gcagtaccat cgtataaagc
     7801 agtatccaaa ccacacaaag tgaaacccat tcctctataa gcatgaatag ctggagcatt
     7861 aacattggta acttccaacc acaaatgacc agcacctctt tctctggcga attcagtagc
     7921 caaacccatc aaagctctac caacaccatg acctctatgt tctggagcaa cttcaatatc
     7981 ttcaacagtc aatcttctgt tccaaccaga ataagaaaca acaacgaaac cagccaaatc
     8041 accatcatca ccataagcaa cgaaagttct agaatctgga tcaccatctt caccagcatc
     8101 ggattcatca tcggattcat catctgggaa aaccttagtc aatggtggat caactggaac
     8161 ttctctcaaa gtgaaaccat caccagtagc agtaactcta aaaacagtat cggtagtgaa
     8221 agaaccatcc aaagcttcaa tagcttcagc atcacctgga acagaagttc tgtatctata
     8281 agcagtatca tccaaagtag tagacataat tgtaggatcc ggttgtttat gttcggatgt
     8341 gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag
     8401 tggcaaaccc taacctttta tatttctcta caggggcgcg gcgtggggac aattcaacgc
     8461 gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt aatttttgct
     8521 tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg gatgtatggg
     8581 ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa gactgtcaag
     8641 gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg aggcaagctt
     8701 gatgtgcggc cgcaagtgat tagacttagt ccgttcaaat caagcacaac tctgttcatt
     8761 gtttcaacaa gaattaattc aaaaacaggt tcggtgcata atttgcaaaa aaatattgca
     8821 gcttctgtgg ctcgaacaca gtacctccag atttcaggtt tgaaatactt cagtctgacg
     8881 ctctcccaga tgagctaaag ctgcaataag aaaacccacg ccgggattcg aacccggaat
     8941 cctttgatta gaagtcaaaa gcgataacca tttcgccacg caggcctact tgatgggttt
     9001 gtaaatggtc tactttttca gacctaacag aaattttaat gaaagtcata ttcttataca
     9061 ataaaactgt gtcataaaag cagatattcg actttcgtag attatatagg acccaagaac
     9121 taaaatttaa tgccatatta tgcattttta atctgtaaaa gtgttgtttc caacctatca
     9181 caagtacgtt cttgtaactt gtgtttgtag ggttgcaaat gaatcataac aacatctcaa
     9241 cagaacatgt atagcaaagc ttagtataaa atcagtgttt tgagaggcaa tccaagaatg
     9301 tttacatcaa agtttcaata aatatcgacc gaaactgaaa atctttttag gttattgttc
     9361 acttttttgt aaatatttaa acattttttg gacctaaaaa aatacaagca ccaattacgt
     9421 accaagaagc atctaatcaa ctcccagatc accactatac atttaaaagt cattggtcaa
     9481 taactatact cgagtattgc ctcatcaaag aaacaatcaa atattataga tactcactcc
     9541 atcacgtgat aatttcactg gtatggaaaa gtggaaaatt ttataaaaaa aaatttgatg
     9601 cctttggcat agctgaaact tcggcccaat aggattggag aatatgtttt cgcagcgttc
     9661 ttacaattaa attgtggtgg aagttcgaga cttgcgtaaa ctatttttaa tttggagacg
     9721 gaattccgtc tcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     9781 acttgaaaaa gtggcaccga gtcggtgctt tttttctcga gtttttttat cgagtgttta
     9841 aggataatga taactgaaga gaagaattag ttttgccgcc accgcgggaa gttcctatac
     9901 tttctagaga ataggaactt cacgccggta aacaagtggt attcaagcac aattcagttt
     9961 tctggatctc taataattgc aattgcaatt gcttcacata ctctgtttat aattcagttt
    10021 ctttctcttg atgttactga tatattaagt taataaatac caataacttt ttttcaaaaa
    10081 tcttctattt tcctttcaat tgattattta aacacgaatc gttaataagc tgtgattgct
    10141 ttttcttttt ctttttcttt tctttgttca agtttgaaaa aagtaaaaaa aaaggtttct
    10201 cttgttcgat gtagaattca gtgggttata gttgtaatga tatatggaat aaaaagaaag
    10261 aaagaaagag agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
    10321 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10381 acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10441 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10501 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10561 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10621 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10681 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10741 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10801 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10861 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10921 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10981 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11041 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11101 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11161 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11221 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11281 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11341 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11401 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11461 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11521 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11581 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11641 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11701 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11761 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11821 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11881 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11941 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12001 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12061 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12121 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12181 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12241 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12301 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12361 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12421 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
    12481 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    12541 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    12601 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    12661 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    12721 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    12781 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    12841 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    12901 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
    12961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    13021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    13081 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgag gcctgcatgc
    13141 ggtaccatga agaatgctga atcacttgat aggatttagt tccattatgg tactaagaag
    13201 taatattact tctatgaaaa ggttgaatcc gagatcaaaa ttcatctttt catatactct
    13261 ggagaaaaaa aaaaaagtaa ttgtagtaag aatgacaagt atcagttgag aaaaaaagta
    13321 acaaagagaa agctcggagg aggctcccca aaggttttat caccaatggt ggaaccacta
    13381 acccaagaac agaaaaagca ttgatatttt gaaaaaatac aatactcatc ttttaacccg
    13441 aaccttttct tgtcctttaa aacttttatc tcatatctgt ccaaagtcat attgtcttac
    13501 tactttctct ccttcatcgt ccttcctgcc actactacca ctgggagttt cgttcttctc
    13561 gatacggatc ctgaagttcc tatactttct agagaatagg aacttcatta gctttacttc
    13621 ctgcactagc agtggttgga tcaacagaat cttcataatc atcaaaatcg tcttttgaag
    13681 accccccgtt tgatgtatgg ccctgtcttt tcatcaaact ttttatatag ttgactgaac
    13741 tgaggctaaa tatgtgatca tcttcactat agacaatctt tctcttattt gcaccaccgc
    13801 caccaccagt ctttgagaaa ttctcaaaac cttttacgat attaccaagc gggctctctt
    13861 cgaaataatc tatctctttt tgatatatcg aatcctctag cgtggttagc tttctagtta
    13921 gttcttgctt cttaagaatt tgctggatta gtttattttt caattcaacg tatttctcag
    13981 agtcatcttt agattttgat gaagatgtgc gttcattcgc tatatccttc ttggtcgtgt
    14041 cttttcgatc ctccttggct ggcactgaac tcgtcttttt tggcgttgct gttccagaca
    14101 gacttatctc attagatttg gaacttgtgg gtttaacatc atttgcatct ttagtagaca
    14161 tgattgtgta ataccgtgat tgtttgtttt gaaaggtctg tcatatttct atcaatttca
    14221 aaacaaaatg ttcatcagaa aaaagccaaa aatgtctctt ctagcttctt agtggtgtcg
    14281 caaaatacac aatgtcgctc aacaatccac attcccggcg catagctcaa atcacatgac
    14341 tacagctaac aattacacaa aaaaattctc ttttcgatgt agcaactatc ttcaactaaa
    14401 acattttctc cttcggccca tgattgtcct ccgggtcgac agcaagccgt tacaattgag
    14461 atggaaagcg acctaccttc actcgataag gtgcttaatt gtacttcata taaatctggc
    14521 ccggatctaa acaaatgagt tccattaagc cgtgggttct caattagggt ttttgttttt
    14581 gatttagaaa aaagagatca agatttgttt acaggtgatg ccttttttta gaacttatgc
    14641 gttgcaaaag ttgactaacg atttctataa ggtgatccac actaattata caaacgtaca
    14701 aacagacata cttttcctgc gttcacctga tgttggccag atttctctct tcattgcata
    14761 gaacataacc acactagggc aacagaaaaa aaaaaaaaaa gtgcatcggg aagttgtgtt
    14821 ccattcatta tatgtctact actgcatatg agtagcccac ccaccaccac catagtaagt
    14881 ttttgtgtat gcgcgccgtc aggttatttc atttctgaat ttttcaacca ccttactccc
    14941 tttattgttg attgacaatt ttgctcacag taagatcttt tagac
//
