LOCUS       sequence-341097-        9247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A plasmid containing the codon-optimized OsTIR1, which is under the 
            control of adh1 promoter. It served as one of the F-box protein 
            expression plasmids in AID system. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL
  TITLE     An improved auxin-inducible degron system for fission yeast.
  JOURNAL   G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 
            10.1093/g3journal/jkab393.
  PUBMED    34849776
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2022-01-4"; volume: "12(1):jkab393. doi"; 
            pages: " 10.1093/g3journal/jkab393"
COMMENT     Sequence Label: pDB5050
FEATURES             Location/Qualifiers
     source          1..9247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     327..346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     580..597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    714..735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(750..755,756..773,774..780)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    788..804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     793..815
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     812..828
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     812..828
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      904..913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(1691..1878)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     1799..1823
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(1905..3632)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     promoter        complement(3647..4388)
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             complement(4466..5260)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRQFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     primer_bind     complement(6287..6306)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6288..6306)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6313..6330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6313..6329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6322..6344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6470..6898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6557..6576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6767..6788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7242..8036
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7296..7315)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7906..7925
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(8054..8122,8123..8914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8272..8291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9085..9247,1..426)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
       61 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      481 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      541 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      601 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      661 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      721 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      781 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      841 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattgccctt
      901 gcggccgcca tggatcctaa ctatgtcttt tagacggttc aaactccctc tcagcgtcgt
      961 gcaaacacac agtttcagaa gaacattact cgtcgtgtaa aaacaaattc aaaagaacgt
     1021 tatgttgcca aacatcctcc taccaagatt cctcgtaaca ttgccagtaa gtaagaattg
     1081 atcctattgt tagcaacttt ggcttgtgtt tcatactgac aatgcatctt agtgtttttt
     1141 attcttctca tgtcaggagg aattattttg ggaatactta gatttctttt gacgtttttt
     1201 tcttaagaac atgtgattgg agcaatttta aaacctattt gcaccgatat tgtgtattat
     1261 actccgagaa aaagtatact agttttgaat aataaagctt gtgatattga cgaaactttt
     1321 tgacatctaa tttattctgt tccaacacca atgtttataa ccaagtttta tcttgtttgt
     1381 ctacatggta ttttacattc atctacatac atctttcatt ggctttgtac atagttatca
     1441 ttacaagtct aaaaaaattc actcttttct tattcaatgt caatccaaga gaaaagattg
     1501 tggtaatgtt gtaggagcat gtttaataaa ttactatagc aaattacttt ttattcccaa
     1561 ggtgtttatc tataatagtt aatattttag tcgctacata aaattttacc aaagagtact
     1621 tgtatactaa ttctaaatgc cttctgacat aaaacgccta gatattaccc tgttatccct
     1681 agcggatctg ccggtagagg tgtggtcaat aagagcgacc tcatactata cctgagaaag
     1741 caacctgacc tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga
     1801 gtcactttaa aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat
     1861 aaatcataag aaattcgctt atttagaagt ggcgcgccag atctttaaag aattttaaca
     1921 aagttgggag catcgtcacg ggcaccggca gtggtacgat aaacatataa tttttcgacc
     1981 ttgggcaaat caccgtggtt ctcttccatt tcattagaac cgtcgcgttc gttaatgacc
     2041 tcaacgttca acataggcat cttggaggcc aaaacttggc atcctttcaa ggtgacattg
     2101 caggaagaca tccacaagct acgcatagtt tcatagcggg cgaaattgcc taaaagtgcg
     2161 gcatcaccaa aaggggaatc acgaatttcc aatttacgga gatttttgca gccattcata
     2221 acatgcatca tgcccttgtc ggagtcacct gcaaaagcaa tggatagcat ctctaattgc
     2281 ttggcgtatt taccaatata cataaagact ttatcggtga gcaaaccact aatagataag
     2341 cgttgtaagc ctttgcattc acggacaata gcaccgaatc cttcatccaa aggttgagaa
     2401 gtgactacat caggcttacc aggctctagt atacaaaggc ggaaacgagt aaaattaggg
     2461 cagtttttag caacggtaac aagagctgcg ttggtcattt gatggcagaa gtaaaggaga
     2521 gagttgagtt tggggcaacc taaactaaca gccactagtc cctcttcagt aacggcagag
     2581 taaccagcga catagaagtc agaaggaaaa acgcgcaatt cttgaagatc cttgcatgag
     2641 gaagccacga cttgtaggcc cttatcagaa atacagtcga gaacccaaag acgttggagc
     2701 ttaacacaac ggctaatcat tttggtaaga tcactagcgt caagagtagg agcgtagcta
     2761 aggttaagac cagtcaattg ggcgcaaaga ggataaatga atgacaggca aacaggagaa
     2821 gcatcccaaa aaccagacaa ggatctaagc attttacact tttctaaagc ggacgttaac
     2881 ttaaagtaag actcagtttg aaaatcatct gtcaagttgc cagtacccaa gtcctcaaga
     2941 tttggtgtac gcaataaaat cttagccaaa gtatcaacag agacagaacg gtttaatcgg
     3001 agacttctga gattggggga tcggctgact aaacgctcca atgatccagc attgacttca
     3061 cccttaatgc aagcgaagtt taacgatact aaggaagtac aagaatccgg gaagcaagaa
     3121 agccaacgag gaccacgatc ttcaacctcg ttttcctgta aatcaagctc tcggagcaac
     3181 ttacaatgag atgcgacagc cgctaaacca tcagtagaga aaccttcaca gctaatgagt
     3241 acgagggcac ggaatctggg gaaagaacga gccaatagtt ctagcgactc atcggataca
     3301 accatacgct tcatacgtaa ttcctcgaga ccatgacaac cccgcgctgc agcttcaatc
     3361 catggtccgg catagccacc ccaatcggga gggaccaaat tgaaatcagc aaaatgaggc
     3421 tttcccttga ctgtaagtgc tcgaacattt ggaaagcgag cggcaacacg accagcacga
     3481 actgcgtaac agttacctac gaaaacaccc cgacgggaca agcgctcaat ctcataccaa
     3541 actttacaaa ctagtgaaac agtgttacga tcacgttgag caggtaaaaa gctaaaaata
     3601 tgttcgacaa cttcctcagg gaaataagtc atggatccgc tagcggaatt ctcttgctta
     3661 aagaaaagcg aaggcacctg tccaccaccc actatttata ccatgggagg cgtcaaaccg
     3721 agtttttgcg aaaggattat cgcacctctc cccaagtaac atcggcaaag caaaaacgga
     3781 gcggaagacc aatgagacgc ggaaggggag cgccgaatga ggaaagcgag agagaaagaa
     3841 gtggatggaa tgattccccg ccaaagtgga cacatttcgg gaatcttcag gaagcatgga
     3901 tcaccttatg gacaagaaga taatccggaa gccaatagga gggcgacagg gggtcagcga
     3961 taagcgtcgg aaaaaacttt atgatatggt aaatacgtag gaggttacgg tgcagaggag
     4021 gggccggtag cgagtgatag cgagggaaag acgatcgcta tcgggccgta aggaggaaat
     4081 gggaggttgg gggaaggatg cgttggaatg cggagtagag aaattgaaga gagagagagg
     4141 acacacctag agagaaagaa atggatatag agaaagagaa aaaataagag gaatacggat
     4201 acgatggagt gcgttgaatg agagaaaaga aaagaaaagg aatgataaga gaaggaatga
     4261 aaattgtttt gttttgtttt caattgcttt cttttgtttt ctttgtcttt gttcttcctt
     4321 cccttgtccc gatgctgttc ttctcaccag cttccgcatg atagccattt tcttagttgt
     4381 tgtagggcat gcctaggaaa acaaacgcaa acaaggcatc gactttttca ataaccaacc
     4441 aaaaaaattt tacattagtc tttttttaat gctgagaaag tctttgctga tatgccttcc
     4501 aaccagcttc tctatatctc ttggcttcga caacaggatt acgaccagct ccatagactc
     4561 cacgaccaac aatgatgata tcgctaccgc agtttacaat cacttcttca ggagtacgat
     4621 attgctgtcc cagcccgtct cctttaacat ccaagccgat accaggggac atagttatgt
     4681 agtcgctttg aaggttagga aattgacgac cagctataaa gccaaagcaa aaatcggtat
     4741 gcttctcaaa ccattctaag gttttctctg tgtaggaacc agtagccaaa gagcctttgg
     4801 aagacatttc agccaaaagc aagagaccac gtcccaaagg taaaccaact tctttgaggc
     4861 cttgtataat accctcgcct ggcactgtat ggcaatttgt gatatgagcc caagaagcaa
     4921 ttttgtacac accagatgca tattgtagct tgacggtatt tccaatgtct gcgaatttgc
     4981 gatcctcaaa gataagaaaa cgatgctttt tacctaaggc caccagtttt tctaccatat
     5041 cctggtcgaa atcctcgaca acgtcaatat gtgtcttgat aacacagaca tagggtccaa
     5101 ttttatctac caattctaag atttcggatt tcttcgtcaa atcgaccgcg actgacaagt
     5161 tgctttgctt ttcttccatc aaagccaaca attccttggc aatgggattt ttcatcccct
     5221 cagctctagc tgaatagctt tgaaatactc tagcatccat aactttgctt ttaaaccttt
     5281 aatttcgatc cgagcaaaaa agaggttctt ggtaggacaa tacggtaaga aaacacgaca
     5341 tgtgcagaga tgccgacgaa gcatagttaa actgggatgg taaaatcaat taagaattta
     5401 taaagacaaa attgtataag tctctaaaac atcttaatta tacctcacag aactatctaa
     5461 aatatattca caaagtgcaa acattatcat gaaaaagaac cattttaatt taaagcaagg
     5521 gcattaaggc ttatttacag aatttcttac ttttgtaaag attataaggc tgattatctt
     5581 tttcaccatg ccaaaaatta cacaagatag aatggatgtt tgaaattaaa cgtgagtata
     5641 caaacaaata cactaggtaa atcgaaacat ttttttctcc attaagtaac aaattcctat
     5701 ttagagaaag aatgctgagt agagtaaata atctatacaa acttttttaa cacaaatgca
     5761 tacatatagc cagtgggatt tgtagctaag cttcaggagt tttatccatt taatgtatgg
     5821 aatcaaaatt taaagcttct gtcaaagttt aacaatattt cttttggttt aaatcaaatc
     5881 ttccatgcga ttaagaagat agatgctgaa caaaagaagc acatggataa ccacaaaagc
     5941 agtttgctca tgggtacaac catgaatttt tttttcgaca atgattcaaa gaccagtata
     6001 tccaatacgc atcctagaat ctagtcaaag aagaacctaa agtagagatg caaatgcgct
     6061 aaaaagagtg gatataaatt caatatcatt tataaaacaa cttcttccat taaaaattcc
     6121 ttgggcaaaa caaaagttcc aatcataaaa agttaataag ttctgagttg tgtcaaatct
     6181 gacatggcat tcctcaataa tgacactcac gcggccgcaa gggcaattct gcagatatcc
     6241 atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct atagtgagtc
     6301 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6361 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6421 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     6481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6841 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6901 aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc
     6961 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
     7021 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
     7081 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     7141 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
     7201 gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     7261 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7321 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7381 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     7441 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7501 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatcccacc
     7561 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7621 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7681 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7741 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7801 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7861 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7921 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7981 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattg
     8041 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8101 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8161 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8221 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8281 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8341 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8401 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8461 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8521 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8581 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8641 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8701 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8761 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8821 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8881 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8941 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9001 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9061 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9121 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9181 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9241 gtagccg
//
