LOCUS       sequence-341099-        9247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A plasmid containing the codon-optimized OsTIR1-F74A, which is under
            the control of adh1 promoter. It served as one of the F-box protein 
            expression plasmids in AID system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL
  TITLE     An improved auxin-inducible degron system for fission yeast.
  JOURNAL   G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 
            10.1093/g3journal/jkab393.
  PUBMED    34849776
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2022-01-4"; volume: "12(1):jkab393. doi"; 
            pages: " 10.1093/g3journal/jkab393"
COMMENT     Sequence Label: pDB4695
FEATURES             Location/Qualifiers
     source          1..9247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..50)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(204..792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(284..303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(963..1754,1755..1823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1586..1605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1841..2635)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1952..1971)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2562..2581
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(2979..3407)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3089..3110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3301..3320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3533..3555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3547..3564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3548..3564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3571..3590
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3571..3589
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4617..5411
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRQFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     promoter        5489..6230
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     terminator      7999..8186
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8054..8078)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     regulatory      8964..8973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9049..9065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9049..9065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9062..9084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9073..9089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9097..9103,9104..9121,9122..9127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9142..9163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
       61 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1561 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1801 acacggaaat gttgaatact catactcttc ctttttcaat tcagaagaac tcgtcaagaa
     1861 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     1921 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     1981 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     2041 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     2101 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     2161 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     2221 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     2281 catcagccat gatggatact ttctcggcag gagcaaggtg ggatgacagg agatcctgcc
     2341 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     2401 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     2461 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     2521 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     2581 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     2641 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     2701 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     2761 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
     2821 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
     2881 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
     2941 gcttccttta gcagcccttg cgccctgaat tttgttaaaa ttcgcgttaa atttttgtta
     3001 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3061 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3121 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3181 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3241 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3301 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3361 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     3421 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3481 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3541 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggc
     3601 cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa ttgcccttgc
     3661 ggccgcgtga gtgtcattat tgaggaatgc catgtcagat ttgacacaac tcagaactta
     3721 ttaacttttt atgattggaa cttttgtttt gcccaaggaa tttttaatgg aagaagttgt
     3781 tttataaatg atattgaatt tatatccact ctttttagcg catttgcatc tctactttag
     3841 gttcttcttt gactagattc taggatgcgt attggatata ctggtctttg aatcattgtc
     3901 gaaaaaaaaa ttcatggttg tacccatgag caaactgctt ttgtggttat ccatgtgctt
     3961 cttttgttca gcatctatct tcttaatcgc atggaagatt tgatttaaac caaaagaaat
     4021 attgttaaac tttgacagaa gctttaaatt ttgattccat acattaaatg gataaaactc
     4081 ctgaagctta gctacaaatc ccactggcta tatgtatgca tttgtgttaa aaaagtttgt
     4141 atagattatt tactctactc agcattcttt ctctaaatag gaatttgtta cttaatggag
     4201 aaaaaaatgt ttcgatttac ctagtgtatt tgtttgtata ctcacgttta atttcaaaca
     4261 tccattctat cttgtgtaat ttttggcatg gtgaaaaaga taatcagcct tataatcttt
     4321 acaaaagtaa gaaattctgt aaataagcct taatgccctt gctttaaatt aaaatggttc
     4381 tttttcatga taatgtttgc actttgtgaa tatattttag atagttctgt gaggtataat
     4441 taagatgttt tagagactta tacaattttg tctttataaa ttcttaattg attttaccat
     4501 cccagtttaa ctatgcttcg tcggcatctc tgcacatgtc gtgttttctt accgtattgt
     4561 cctaccaaga acctcttttt tgctcggatc gaaattaaag gtttaaaagc aaagttatgg
     4621 atgctagagt atttcaaagc tattcagcta gagctgaggg gatgaaaaat cccattgcca
     4681 aggaattgtt ggctttgatg gaagaaaagc aaagcaactt gtcagtcgcg gtcgatttga
     4741 cgaagaaatc cgaaatctta gaattggtag ataaaattgg accctatgtc tgtgttatca
     4801 agacacatat tgacgttgtc gaggatttcg accaggatat ggtagaaaaa ctggtggcct
     4861 taggtaaaaa gcatcgtttt cttatctttg aggatcgcaa attcgcagac attggaaata
     4921 ccgtcaagct acaatatgca tctggtgtgt acaaaattgc ttcttgggct catatcacaa
     4981 attgccatac agtgccaggc gagggtatta tacaaggcct caaagaagtt ggtttacctt
     5041 tgggacgtgg tctcttgctt ttggctgaaa tgtcttccaa aggctctttg gctactggtt
     5101 cctacacaga gaaaacctta gaatggtttg agaagcatac cgatttttgc tttggcttta
     5161 tagctggtcg tcaatttcct aaccttcaaa gcgactacat aactatgtcc cctggtatcg
     5221 gcttggatgt taaaggagac gggctgggac agcaatatcg tactcctgaa gaagtgattg
     5281 taaactgcgg tagcgatatc atcattgttg gtcgtggagt ctatggagct ggtcgtaatc
     5341 ctgttgtcga agccaagaga tatagagaag ctggttggaa ggcatatcag caaagacttt
     5401 ctcagcatta aaaaaagact aatgtaaaat ttttttggtt ggttattgaa aaagtcgatg
     5461 ccttgtttgc gtttgttttc ctaggcatgc cctacaacaa ctaagaaaat ggctatcatg
     5521 cggaagctgg tgagaagaac agcatcggga caagggaagg aagaacaaag acaaagaaaa
     5581 caaaagaaag caattgaaaa caaaacaaaa caattttcat tccttctctt atcattcctt
     5641 ttcttttctt ttctctcatt caacgcactc catcgtatcc gtattcctct tattttttct
     5701 ctttctctat atccatttct ttctctctag gtgtgtcctc tctctctctt caatttctct
     5761 actccgcatt ccaacgcatc cttcccccaa cctcccattt cctccttacg gcccgatagc
     5821 gatcgtcttt ccctcgctat cactcgctac cggcccctcc tctgcaccgt aacctcctac
     5881 gtatttacca tatcataaag ttttttccga cgcttatcgc tgaccccctg tcgccctcct
     5941 attggcttcc ggattatctt cttgtccata aggtgatcca tgcttcctga agattcccga
     6001 aatgtgtcca ctttggcggg gaatcattcc atccacttct ttctctctcg ctttcctcat
     6061 tcggcgctcc ccttccgcgt ctcattggtc ttccgctccg tttttgcttt gccgatgtta
     6121 cttggggaga ggtgcgataa tcctttcgca aaaactcggt ttgacgcctc ccatggtata
     6181 aatagtgggt ggtggacagg tgccttcgct tttctttaag caagagaatt ccgctagcgg
     6241 atccatgact tatttccctg aggaagttgt cgaacatatt tttagctttt tacctgctca
     6301 acgtgatcgt aacactgttt cactagtttg taaagtttgg tatgagattg agcgcttgtc
     6361 ccgtcggggt gttttcgtag gtaactgtta cgcagttcgt gctggtcgtg ttgccgctcg
     6421 ctttccaaat gttcgagcac ttacagtcaa gggaaagcct catgctgctg atttcaattt
     6481 ggtccctccc gattggggtg gctatgccgg accatggatt gaagctgcag cgcggggttg
     6541 tcatggtctc gaggaattac gtatgaagcg tatggttgta tccgatgagt cgctagaact
     6601 attggctcgt tctttcccca gattccgtgc cctcgtactc attagctgtg aaggtttctc
     6661 tactgatggt ttagcggctg tcgcatctca ttgtaagttg ctccgagagc ttgatttaca
     6721 ggaaaacgag gttgaagatc gtggtcctcg ttggctttct tgcttcccgg attcttgtac
     6781 ttccttagta tcgttaaact tcgcttgcat taagggtgaa gtcaatgctg gatcattgga
     6841 gcgtttagtc agccgatccc ccaatctcag aagtctccga ttaaaccgtt ctgtctctgt
     6901 tgatactttg gctaagattt tattgcgtac accaaatctt gaggacttgg gtactggcaa
     6961 cttgacagat gattttcaaa ctgagtctta ctttaagtta acgtccgctt tagaaaagtg
     7021 taaaatgctt agatccttgt ctggtttttg ggatgcttct cctgtttgcc tgtcattcat
     7081 ttatcctctt tgcgcccaat tgactggtct taaccttagc tacgctccta ctcttgacgc
     7141 tagtgatctt accaaaatga ttagccgttg tgttaagctc caacgtcttt gggttctcga
     7201 ctgtatttct gataagggcc tacaagtcgt ggcttcctca tgcaaggatc ttcaagaatt
     7261 gcgcgttttt ccttctgact tctatgtcgc tggttactct gccgttactg aagagggact
     7321 agtggctgtt agtttaggtt gccccaaact caactctctc ctttacttct gccatcaaat
     7381 gaccaacgca gctcttgtta ccgttgctaa aaactgccct aattttactc gtttccgcct
     7441 ttgtatacta gagcctggta agcctgatgt agtcacttct caacctttgg atgaaggatt
     7501 cggtgctatt gtccgtgaat gcaaaggctt acaacgctta tctattagtg gtttgctcac
     7561 cgataaagtc tttatgtata ttggtaaata cgccaagcaa ttagagatgc tatccattgc
     7621 ttttgcaggt gactccgaca agggcatgat gcatgttatg aatggctgca aaaatctccg
     7681 taaattggaa attcgtgatt ccccttttgg tgatgccgca cttttaggca atttcgcccg
     7741 ctatgaaact atgcgtagct tgtggatgtc ttcctgcaat gtcaccttga aaggatgcca
     7801 agttttggcc tccaagatgc ctatgttgaa cgttgaggtc attaacgaac gcgacggttc
     7861 taatgaaatg gaagagaacc acggtgattt gcccaaggtc gaaaaattat atgtttatcg
     7921 taccactgcc ggtgcccgtg acgatgctcc caactttgtt aaaattcttt aaagatctgg
     7981 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     8041 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     8101 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
     8161 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatctagg
     8221 cgttttatgt cagaaggcat ttagaattag tatacaagta ctctttggta aaattttatg
     8281 tagcgactaa aatattaact attatagata aacaccttgg gaataaaaag taatttgcta
     8341 tagtaattta ttaaacatgc tcctacaaca ttaccacaat cttttctctt ggattgacat
     8401 tgaataagaa aagagtgaat ttttttagac ttgtaatgat aactatgtac aaagccaatg
     8461 aaagatgtat gtagatgaat gtaaaatacc atgtagacaa acaagataaa acttggttat
     8521 aaacattggt gttggaacag aataaattag atgtcaaaaa gtttcgtcaa tatcacaagc
     8581 tttattattc aaaactagta tactttttct cggagtataa tacacaatat cggtgcaaat
     8641 aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa gaaatctaag
     8701 tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga tgcattgtca
     8761 gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac tggcaatgtt
     8821 acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat ttgtttttac
     8881 acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg gagtttgaac
     8941 cgtctaaaag acatagttag gatccatggc ggccgcaagg gcaattccag cacactggcg
     9001 gccgttacta gtggatccga gctcggtacc aagcttggcg taatcatggt catagctgtt
     9061 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9121 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9181 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9241 ggggaga
//
