LOCUS       sequence-341116-       11852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carboxysome operon with truncated CsoS2 (deletion of amino acids 
            2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCB' CsoS2 Delta-NTD
FEATURES             Location/Qualifiers
     source          1..11852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             3257..3268
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(3278..3303,3304..3309,3310..3326,3327..3332,
                     3333..3351))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    3333..3351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3372..3390)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3569..4363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3623..3642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4233..4252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4394..4488
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4576..5164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5065..5084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5340..5444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5445..6068
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5518..5537)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(6241..6327)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7377..7394)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        7440..7495
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    7441..7459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7471..7489
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 tgccagcata ccagggcctg taatgcgacc tgagggcgca atgtcgttac ctgtaatgcg
       61 attctgagcc gagcgagctg gggtttgaat actaaagtca gtaggcatta cttcacctgg
      121 tgcaaagttt tgttctggct gattggtgtt cttaaaaccc attgactgaa cattgccagt
      181 ttggttgaag cgtggactag tgggaaggca acctgtctgc tgcgcgccaa cataggcgtc
      241 accgctgatg agttgctgtt caccgatttc atttcctgtg accaaatcac tggcgtcaac
      301 tgaagtgccg ctaataattt gtccttcaca ggtcaatgat tgttcagttg attgagcttc
      361 gggctctggg gtgctcgtac aaaacggttc gaaatgttca cgaccgtagt actcattacc
      421 ggtgacgggc atgcacccgc cgcgctcgtc accggacatc tttggggcat gatctagctg
      481 ttggccagat actgaggtgc cgcgaagggt gcgcattgag cgcacttttc ccacgccacc
      541 accgcagatt ttgctttggc catactgaga gcctgtaacc cgagagcatg aacctggttc
      601 gttacctgta accagttcgg aacgatcaac caagttacca gtcacagact gacccttgtt
      661 cgtgcgatct tggccaacct tgaacgggct gcgttgaggt tttgtgtcac aaaaggattg
      721 gaattgctcg ttactgagat actcggtgcc tgagattgaa cgacacgaac cgctttcatc
      781 accagttacg cgagtagagc gaccgatttc cgtgccggta acgtcagagc ccgcaaaggt
      841 gtgggttctg gctacttttg caggtgctcc gttgatcgtg agtcgcgcaa gaccttcgtc
      901 tgcgtactgc gagccggtaa ttgtgcgttt tgcacccgat tcgttacccg taacagaaga
      961 gggcctgaat tcatcactgc cactgaccac ttgatgcgtt cgtgctgtgg cgccgaacat
     1021 gactttatcc gcttgtgaag gctttgcggc tgttccacat agagaaaagt gagcttcatt
     1081 actcatgtat tcggtgccgg taacgttacg gcagacaccg gattcgttgc cagttacctt
     1141 ctcggtccgt gaaacttccg taccgctaac agggcgacca cgagccgttg tggtcacatt
     1201 gaccttcgtc gcatttggct tggggctggt attgcaaaaa gaggtgaatt gctcagtacc
     1261 tacgtactcg gtgcccgtga ctgctcggca agtgcccggc tcgtttccag tcacatgaga
     1321 tttacgatcc acttgcgtgc ccgtaacaga tccgccggat agggtgtgac cggtttcaac
     1381 cttttttggc gccatgtttg accccatctt gaataagtgc tgtgcgtaga gaaacgcaca
     1441 gcgcaatgac agacttgact tagttgccgc ggtagaccac gaaggccaga cctaagcttt
     1501 gcgcatagtt gtcataagcc accagtttga cctggtgtgt tggatacgca ctacgacacg
     1561 cttcaatttc agccaacacg ttgtcgacat tttgttcgcc gaagaaggga agtttccaca
     1621 tgtaccaata ttggttcatg gagtttttca cttctacgtg ctcaatgccg gggctccagc
     1681 cttgagcaat tgcgtacttg atttgagcgc ggatgcgttc cgcgttcatg ggtggaagat
     1741 aagagaaagt ctcatatttg aggctttgct tgtaatcctg catttcagcc atggttactc
     1801 accttagtat gttgtgtggt acgagggatc aacgattttg agtgtcgagt ttgtcgacag
     1861 tgtcaaattc gaatttgatc tctttccaag tttccatggc aatcttcagt tctgggctgt
     1921 gctgtgcagc agcagtcaga atttctttgc cttctttttc gatatcgcgg ccttggttac
     1981 gcgcttctac gcaggcttcc agagcaacac ggttggcagc agcaccggca gcgttgcccc
     2041 atggatgacc cagcgtaccg ccaccgaatt ggaggacaga gtcgtcacca aagatgttaa
     2101 ccagcgcagg catgtgccat acgtgaatac caccagaggc cacagcgaat acgcctggca
     2161 ttgaacccca gtcttgatcg aagaagatac cgcgcgagcg atcttcaggg ataaacgatt
     2221 cgcggagcaa atcaatccag cccagagtag aggcacggtc gccttccagt ttgccgacaa
     2281 cggtaccggt gtgcaggtga tcgccacccg acaaacgcag aatcttggtc agaacacgga
     2341 agtgaatacc gtggttcggg ttacggtcga taaccgcatg catcgcacgg tggatgtgca
     2401 gcagtacgcc gttgtcttga caccacttgg ccaagccagt gttggccgtg aagccaccgg
     2461 tgatgtagtc gtgcataatg attggcgcgc caatttcttt ggcgaattct gcgcgcttgt
     2521 acatttcttc aggagttggc gccgttacgt tgaggtaatg gcctttgcgt tcgccggttt
     2581 gggcttcagc agtttcggtc gcgtcttgta cgaacaagaa gcgatcgcgc cagcgcatga
     2641 acggctgaga attgatgttt tcatcatctt tagtgaagtc gaggccgcca cggaggcact
     2701 cgtatacggc acggccgtag tttttcgcag acaaaccaag ttttggcttg atggtgcaac
     2761 ccaacagtgg gcgaccatat ttgttcatct tgtcgcgttc gacttgaata ccgtgcggtg
     2821 ggccgccaca ggttttaacg taggcgagtg ggaagcgaac atcttccaga cgcaggccgc
     2881 gtaccgcttt gaagccgaac acgttaccaa ccagtgaggt aaacacgtta acaactgacc
     2941 cttcttcgaa caggtcgatt gggtaggcga taaaggcata gaatgccgca tcgtcaccgg
     3001 gtacgtcttc aatgcgatag gcacggcctt tgtagtagtc catgtcggtc agcaaatcgg
     3061 tccacacggt ggtccatgtg ccggttgaag attctgctgc aaccgcggct gcggcttctt
     3121 cgcgatcaac acccggttgt ggggtgattt tgaagcatgc aaggatgtcg gaatccaacg
     3181 gtgtgtattc cggcatccaa taggtctgcc ggtattcttt tacaccagca ctatactttt
     3241 taactgccat ggtacctttc tcctctttaa tgaattcggt cagtgcgtcc tgctgatgtg
     3301 ctcagtatat ctatcactga tagggatgtc aatctctatc actgataggg actcgaggtg
     3361 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3421 ttcttagacg tcggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     3481 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
     3541 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3601 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3661 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3721 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3781 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3841 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3901 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3961 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4021 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     4081 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4141 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4201 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4261 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4321 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     4381 gagagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     4441 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcactagt
     4501 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4561 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4621 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4681 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4741 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4801 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4861 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4921 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4981 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5041 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5101 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5161 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5221 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5281 agctgatacc gctcgccgca gccgaacgcc ctaggtctag cgcgaattgt cgagggaaac
     5341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgtct agattagata
     5461 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     5521 caacccgtaa actcgcccag aagctaggtg tagcgcagcc tacattgtat tggcatgtaa
     5581 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     5641 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     5701 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     5761 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     5821 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     5881 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     5941 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     6001 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     6061 ggtcttaaca gttcctggat atccggatga aggcacgaac ccagtggaca taaccctgat
     6121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6181 ttattccctt ttgcttggat tctgcgtttg tttccgtcta cgaactgcta gcggcggcgg
     6241 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     6301 tttcgactga gcctttcgtt ttatttgatg cctctagcac gcgtaccatg ggatcccccg
     6361 ggctgcagga attccatcta gacactagcg gcgacgggtg ctgcttggga ggtcaaacgc
     6421 tcataggccg cccgcatttt caggccggtc agcacttgga acagacccgt gccgttgttc
     6481 gaacccgggt aatctttgtg ctgtagcagc aggtgggtca actcaccgta gtactgcgtg
     6541 tcggtcttgc tgaggtggcc ctcgacgtag atcatctctt cgaggttctc gaactgaccg
     6601 tcgacctcaa gcactgagac ttcgtgaccg tagtaggtgt cagggccgta ggctaagcga
     6661 atgcccggat aggagcaggt cagcttacga ccgcagggcg tccactggat ggtcgaacct
     6721 tcgtcgaaca ggtaagccgg ctcaaaacca tcgcggcctt cccgttggat caactgcacc
     6781 cgcagcacct tgcgctcggt gtcattgggg attagctggg tcggcatgac gcggatgacg
     6841 atgtccgcat ggccacgctg gggctcaatg taggccttga agtcagggcg gcgcgcttcg
     6901 atcgaggcga ggacatcttc gtaggagtgg ccgcgttctg ccatatcgcg ttggatcttc
     6961 caagcgattt tgacttcgtc atcgatgtcg aggtaaacgc tgaaatagag cagttcacgc
     7021 acgcgctcgt cgtaaagcgg atgcagaccc tcgatcacaa tgatgcgatt gggttcgatt
     7081 ttttcgggcg gatcgatcaa gccggtttca tggttgtaga tcggcttcat gatcgtttcg
     7141 ccgttcttca acgccttgac ctgttcatac atcaagtcaa agttgttggc gcggggatcc
     7201 aaagccgtta cgcctgcttc cttccggccc ttgcgatcga gactgtgata gtcatccaag
     7261 cagatgaccg tcatcaattc cgtaccaaac aagtcggcaa ggcgatttag gaaggttgat
     7321 ttgccgcaac cggagtcacc ggcaacgccg atcaaaacaa cacgatctgg cttgctgtgg
     7381 tgatggtgat gatgcatgcg gaattccccc tcttactact ggctggcttt ttttcacttt
     7441 tctctatcac tgatagggag tggtaaaata actctatcaa tgatagagtg tcaacaaaaa
     7501 ttaggaatta atcatgattt agaacccttc agcgcgacgc gcacgcgcgt tgatgcttgc
     7561 gattgtggcc tcagccgccc gcatggcttc ttcggtttcc gcttcgctgc ccatcatggt
     7621 caggcgacca aagttaccaa aagcacgtac atcgatcaga gtcacatgag ctgctttctc
     7681 ggcctcgttg gcagcctgaa caatataacc tgccggttct gtttcaagga tgaacatgct
     7741 tttgcccggc aacagcatag agcctttacg caactggcga ttgatcaagg tggcatgatc
     7801 gggggtaatc gctcgaatga tctctttcca tgcgatgcga caaggcagac gatcttcttg
     7861 cgcgtgattg atttctctta aaacggcctc accggatgcg agaacgtcgc tttggtttcg
     7921 gtaatgaatt tccatcgagc caaaagcacg ctcgactacc tgttcgccga gacgaacgtt
     7981 cgtggccttt agcgcaatat cactgaggcg atggacggcc atgcccggcg cgacttcaat
     8041 ccacaagcag gcatcacccg gaacgggaag aaagccttgc gatgatgtcg caagatagga
     8101 tgcaagctgc ggttgaagcg aatcgataaa cgaatagacg cgcaaatcaa tgttgttcat
     8161 gcgcatcttc cctactagac attagctatt cagatttgcg atacaccacg cggaatctga
     8221 gtcttcgggc gtctcgggta ggatgatctc aacttcagaa tgaacgcgcg caatgatgtg
     8281 cgcagcaacc aggccatcgc caacacgttc acaggcgtca gcacccgcac gaacggcagc
     8341 atttactgca cctgtctcac cacgaaccat tacggtcacg taaccgccgc caacaaatga
     8401 tcgtccgacc agacggactt ccgccgcttt ggtcatggca tctgcggctt caatggcagg
     8461 aaccaatcct cgtgtttcaa tcatgcccag ggcaataccg tgagtcgttg ccataacaga
     8521 tcctctaaaa ctagtgttga gcacgttcag aacgagccca gaaatttggt tagacggttt
     8581 cggcgacggg tgtcgccgaa gtgagccgtc ttaggaatat ctgacttagg cttgtggcgc
     8641 cttaggcaga atgttttcta cttctgagtg gacacgcgca atgatgtgcg cagcaaccaa
     8701 gccatcacca acacgttcgc aagcatcggc gccagcacga acagcggcgt taacagcgcc
     8761 tgtttcgccc cgaaccaata cggtgacgta accgccacca acaaattgac gaccgaccaa
     8821 acgcacttca gccgctttag tcatggcgtc cgctgcttca atcgcaggaa ctaagccacg
     8881 tgtttcgatc atacccagag caataccagt tacatcagcc atttaaataa actcctaagc
     8941 tgtcacttta tgcaaagaac cggaacaagc ctgcgccggt tcgtctttcc caatccttaa
     9001 gcttcagggg ctttcggcag gatgttttcg acttcggaat ggacacgggc aatgatatgc
     9061 gccgcgacca gaccatcgcc gactcgttcg caggcatcag cgcccgcacg aactgctgcg
     9121 ttgacggcac cggtttcacc acggaccaaa acggtcacgt aaccaccacc aacaaattga
     9181 cggccaacca aacgtacttc ggcggccttg gtcatggcat cggcagcttc aatcgctgga
     9241 accagaccac gtgtttcaat catacccagt gcaatacctg ttactgctgc catttcttct
     9301 ctcctcaata aagactcatt caaaatcaac tcatctagcg atggtcaagt tacccagtga
     9361 tcgatgatgc caccaatcgt caaatccgtg agaatctcaa aatcacccac gccaaaccga
     9421 gcggcagagc cgctaatcgt aaagacccaa catccctcgg gaacgccaat gggatcgcaa
     9481 gcgacactga ccttacccgt agcatcctcc atgacacgca aagagatatt tttaagaccg
     9541 ggaatcctgc gtgttgcgat taggtcggaa cgaacgcgca ttacttccat tactcaccat
     9601 tccactgatc aataatcccg ataatcgtca aatcagaggg ataagacttg cttcctgcag
     9661 cctctcgtgc tgccgatgac ccaacgcaca aaacccaatc gcccggtatg cagccaatcg
     9721 catccacggc gacctgcctg ggcgcgcccg gtttctccca taccactaat agtggtttgt
     9781 gacccatatc agcaatacgg tttgttgaaa ccaacgtttt ctcaacttgc atgattttca
     9841 tgcggatgca acctcttcaa tcgggcttcc gatatcctgc gcctgaacgc tcaaacgaaa
     9901 ctgcaataga ttctgctcat ccaaggatga gaaccgctct ttaatggcgt tgtatatccg
     9961 tcttgctttg accacggttc tttctctgga gcctggaaca ttggggtcat agcgatagtg
    10021 gatggcaatc ggaatcggta aacctcgact caaattaagt ttggtgaaaa ttttgattcc
    10081 cacatccaca tcaatcgcat tttcttcaac cgtatccaaa tgcgcgtagt aggctaaatt
    10141 ccggatttga acttcatcaa aaccatcacc aacactgata tatcgctcag catgaccaat
    10201 atcattgggt ggatagcgac caccatgacg atttactacg taatcgattt gcgagaggtt
    10261 gttgatcaaa agctgaccaa taaaacgacg catcccctcg ctggctactc catttccttt
    10321 agcccaacca tcgctgcggt ttgcgttgag aattgcttcg tgaataatca cacgagcctc
    10381 atccggcgcc agatgtagtg tttgcgcata agttactgtg ttgtcaacat aacgatacgg
    10441 attcaaaaaa cctttgctat ccggaatatg aacgcgaatg gcatccgtat ccgtatcaac
    10501 gccaatcagt aaaatatcga tacttgcgcc gcagcaatgg gcattttcaa ccgcttctct
    10561 gaatttcatc agctgggtta aagccgcttc cagtgctgca cgatcattac tgccgtgtgc
    10621 cgcgcagcca gagtgtgtcg ggtcagaggt gctgaaatga tacacagcaa ttttcaggta
    10681 tcgtgttggc tgatccgctg tatttggaac accttcacga taacggcgga gttcattttt
    10741 ctcccactgt gccagatcat cttcaatatc gaacatcgag cctgcgtagg ctttacgata
    10801 ggtgaatgcc gtaaggggca aacgcaatat gtaaggcagt aaccctttga gacgaccatc
    10861 ggcacaaggg cttatgtcca ctgcgtggaa cccgcaatcc aggaagaagc caatggccga
    10921 tgcggaatcg tccttctcag acattatttt acgactgaat tgttccgtgg ctgctgttag
    10981 tgctttagca gtcgcgtagc caaaaatgcc ttttaaattg tgcccactga tccaattatc
    11041 tttgagtaat tcttcgggca gcgtatgtcc aagcgacgac tgggctaatt cttgaacacg
    11101 ggtaacataa tccagaccaa gaggaatgcc agcaacaact tgcaagaaag ggataacgcg
    11161 atcaaaacgg ttcttgacgc tatcctcgca acgacccagt tgttcgttgg cttcaaggtc
    11221 agttaacggg tgccggcagg tacgctcagg caacgttatg caagcaggat ggcggtcata
    11281 ggcagggttt ggtgcttgct caatcaagtc aagccgaggt ttgactgatg agctaacacc
    11341 aaacggtgcg cgttgcttgc ttcgtgtgtt acgggtgttc atgatcgtta cactttactt
    11401 aatcaaccgc gcgcgccgcc ggagtaagtg atcagactac cttgggtgtc attaccacta
    11461 ctgcccgtga tcttggagcc cggcttttca ggttttggca cattgcgatt ggcaaacgcg
    11521 ctggtttcga ccacacgcgc attaccgcgc atggaagggt tgcgaccact cgcccattga
    11581 ccggctgtac cggtgatgtg cttgttaact gaccagtcat cgccagtgat gtggcaacgt
    11641 tctttgcctt cgccactcac acgatccatt tgatcgcttc ttggtgcaca taccaactca
    11701 ggcttttcct gagttgcaac cacttctggc tgcacaacag gtgcttgagc agccttatta
    11761 cggttggcca tggccattgc catgggttgt ggagaaccaa ccaactcgcg cgcagcgtgc
    11821 ctgaattcgg gggttcctgt aatcaagccg gt
//
