LOCUS       sequence-341131-       14585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCM reconstructed after selection for CCMB1 growth on glycerol 
            under ambient air.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14585)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 14585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCCM'
FEATURES             Location/Qualifiers
     source          1..14585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             3610..3621
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(3631..3656,3657..3662,3663..3679,3680..3685,
                     3686..3704))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    3661..3679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3686..3704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3725..3743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3809..3911
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3912..4571
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3978..3997)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      4592..4686
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4774..5362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5263..5282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5538..5642
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5643..6266
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCILEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5716..5735)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(6439..6525)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agtgccggta gtttctcagg cagtcgagct acatggtcga ggatcatgta gtgtttcggg
       61 ccaaaaattc gttcgacata atcatcggct cttggatcga gggtgacgca gaacgtattg
      121 atgccccgtt tccctaattc ctcaacaacg cgcttggtgt cttggcgtag atattggggg
      181 tcgcgcacat cattatcgga aggctcgcca tcggtaagta acagaataag tttcttctgg
      241 ctgggccgtg aatccaacaa atggcccgcg tgtcgcaagg cagtgcccat gcgtgtggag
      301 agttgccccg tcattccggc cagacgagcc ttggcgaggt catcgaaggc gtcatcaaag
      361 tctttgaagc ggaaatactc aacatcgctg cgcccattgg aatcaaaacc atgaatagcg
      421 tagggatcac caatgcgatt caatgcatca ccgagtaatg ctgctgcctc tctagccata
      481 tcaagtacgg ttttttcttc ttcaccggtt tgcacgagat cattggttga ttctgagaga
      541 tcaatgagca aaatcacgga tagatcacgc acatggatac gtcgacgaat gccaatgcgc
      601 gggtccggtt gacgattcat tcgaatatcg atcattgcgc caatggctgc attgagatcg
      661 acttcatcac catcctcgat ttttttctgt cgcacgacac cttgtggctg taagccttcg
      721 atcaggtact tcaaacgccg taccattggg gcgtgctcga acaaaatatt atcaaccacc
      781 gcaggatcgc ccaatttggg tttcttctcc atcagggtcg tccacaatgg tcgctcaagc
      841 tgtgattggt aatcccattc gggataatat cgaggcgaag cagtcggtgg cgtgccataa
      901 agctcattga acgtcgtgcc atcgtcatca aaaagttcgg tggcaagcac ccatatttca
      961 tccgcgcttt ccgccgtggc gtactccaca tccaaggcgt gcagcatctg catcacgcta
     1021 acgtacttgc gcttttcctg aatgctggcc gcggtatgtg cattgctctt gcctgtttcc
     1081 ggtgtttgcc acaaataggt attgtcatca cggtacgggc tacttgggat atcggtacgg
     1141 ctttgaaata ccaggttttg ctgctggatc ttttcggcta gagcgatgcc cagttctcgc
     1201 gccatctcct gatcttccag acgatcaagt gctgctttaa acgcctcacg cgaccattcg
     1261 acaagcgggt gggtatccga tgaattctca tcgcatagtg ccagcgctat ccggtcgaaa
     1321 agtgcgccaa gagttggatt ctctcctgtt cgtgcgtcag cggggagggc agcaaccgtc
     1381 agttggttga gattcctcca caggggaacc aatccaggga aatcggccat tgcgagtttt
     1441 tcgacacgcg cgtcttcaaa taaaccaatg gtcgcgcggt ccagtgggga tgcgttcttc
     1501 gcatcggagt cgagacgcga ataaatttga tgtgctgcgg catgagccgc tgccgcacga
     1561 tagacagtca gaccatcaac tgtttgttcg ccttcaacgg cgtaatcatc atacgcgtcg
     1621 ggtaaattga taaatagttc atcaatgaac ggacggtatc ccgcacggct ttcgaaatca
     1681 cccgaagtgg ggcgtagaaa aaaatcacgc ccccacagtg cgcgtaaata catcaccaag
     1741 cggcgttgta catcaacaaa caatacaccc cttcgctcgc gctgtaacac agcacgggat
     1801 tcaggcgttg caagactaaa gtaggcctct tgggcggcaa aatcggttcg gtgcgcttga
     1861 atcccccaag tgatccaacg ccgcaatccc ccaagcgtca gcacaccaag cagttgctct
     1921 atttgaccaa gaagtggacg taaggctcgg ggggctttgg ccgctatgta atccagtaaa
     1981 gaaagatagg caacgaacag ttcagcctca ccgagccgac tgacagcaac aggtgccgat
     2041 ttgaaaacca ttgctaaaac ttcagcactg gccttggaga acattttaat aacggaagac
     2101 agcagttcga aaactgctgt ttcaccaatt gaccttgtaa caggacctgc cgattcaata
     2161 aacgagataa ctaactcatc accgcgtccc aaagccttaa gattggaaac gctttctaga
     2221 aaagccttga aacccatcgg gctgaacaat cgcgaaagct caatccaatg ggtctcaagg
     2281 acttcagcga tcggttcgcc caagtcttca aaaagagcct gatattcttc agtcgctggg
     2341 ttcatagttt gacgttagtt tcgcgatgtt cggacaaaat caatctggcc atacacggcg
     2401 aggatgcgtc gatgtgtcgt gtgccggaac ctgaatatca tcagccgatg gcttgctgga
     2461 ttttgataca gcaggttttg ttgtcgtcgc acgcgctaca ggctttttcg cagccgtccg
     2521 tgtggcaggt ttggcccctg tcgatgcagg cttggtggtt tcgggtgcag ccgagcgcgc
     2581 ggctggtttg gctggcgttg acgcaggcgc tatcgtgtcc ttgttctcgg tcttgatgct
     2641 tgcactcagt ttatcttcta aattagtcat gacgggataa cctcgttaag gatttgattg
     2701 atttcatcag ccgctgagcg gcctcttgct ccgagctgga aaacgctaac gccttccact
     2761 acggcatttc ggtacacagc gcgtcgtcgc acagaggtag tgagtacggg aacccctagt
     2821 cgctcaatgg ttgtttgcat tgctcgtgtc attgcactgc gcggctctgt ttgattgagt
     2881 accaacgcag cttttagctt ggggttggtt ttgcgtgcgc tttcaatcat ctcgattgtt
     2941 tgagtgctcg cccaaagatc taaaggggag ggcagtacag gaattaatat aaaatcgcac
     3001 tcaatcatca attgacaggt aattgccatg tctagggaag gcgggcaatc cacgacgaca
     3061 aagtcatggt ttggttgcgc caatttaaga ttttgaacgg gatcggtgtg atctgagtat
     3121 aagacgggga cttgagcgtc cgcctctttg cttccccagt caacccaatg cttgagtgcg
     3181 ccttgggggt cggcatcgac cagaccaaca cgaccacgct gcgtcaagcc cgcactgatg
     3241 tttgtgctga tggtcgtctt gccacagcca cctttgaggt tggcgacagc caatgtaaag
     3301 gctttgttgc tggccatggc gttagctata cttggcggcc aagtcattga cttgggtagc
     3361 aaattcaacc tcagactctt gcaatggacc gtcgtccgca gcagatatgg tgacggtcac
     3421 gaattctttg gcaaaatgca agttgggata gttgccaatg gcctcagata aatcagctaa
     3481 atcgtccaaa aatgaacgcg tttgagaata acttccaaaa tcgaagcggc gagttaattg
     3541 aggtggtaat ttacggcatt cccaaccatc aacctcaatc tgatttagct tttctatcgc
     3601 catggtacct ttctcctctt taatgaattc ggtcagtgcg tcctgctgat gtgctcagta
     3661 tctctatcac tgatagggat gtcaatctct atcactgata gggactcgag gtgaagacga
     3721 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3781 acgtcgatat ctggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     3841 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     3901 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     3961 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     4021 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     4081 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     4141 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     4201 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     4261 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     4321 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     4381 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     4441 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     4501 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     4561 gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc
     4621 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
     4681 gagaatccaa gcactagtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4741 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4801 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4861 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4921 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4981 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5041 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5101 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5161 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5221 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5281 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5341 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5401 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5461 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgccct aggtctagcg
     5521 cgaattgtcg agggaaacgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5581 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5641 gtatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
     5701 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gcgcagccta
     5761 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
     5821 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
     5881 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
     5941 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
     6001 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
     6061 ttactttagg ttgcatattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
     6121 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
     6181 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
     6241 aacttaaatg tgaaagtggg tcttaacagt tcctggatat ccggatgaag gcacgaaccc
     6301 agtggacata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6361 aacatttccg tgtcgccctt attccctttt gcttggattc tgcgtttgtt tccgtctacg
     6421 aactgctagc ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     6481 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tctagagtat
     6541 tacaatagct aagaatttcg tcatcgctga atacagttac attttagaac ccttcagcgc
     6601 gacgcgcacg cgcgttgatg cttgcgattg tggcctcagc cgcccgcatg gcttcttcgg
     6661 tttccgcttc gctgcccatc atggtcaggc gaccaaagtt accaaaagca cgtacatcga
     6721 tcagagtcac atgagctgct ttctcggcct cgttggcagc ctgaacaata taacctgccg
     6781 gttctgtttc aaggatgaac atgcttttgc ccggcaacag catagagcct ttacgcaact
     6841 ggcgattgat caaggtggca tgatcggggg taatcgctcg aatgatctct ttccatgcga
     6901 tgcgacaagg cagacgatct tcttgcgcgt gattgatttc tcttaaaacg gcctcaccgg
     6961 atgcgagaac gtcgctttgg tttcggtaat gaatttccat cgagccaaaa gcacgctcga
     7021 ctacctgttc gccgagacga acgttcgtgg cctttagcgc aatatcactg aggcgatgga
     7081 cggccatgcc cggcgcgact tcaatccaca agcaggcatc acccggaacg ggaagaaagc
     7141 cttgcgatga tgtcgcaaga taggatgcaa gctgcggttg aagcgaatcg ataaacgaat
     7201 agacgcgcaa atcaatgttg ttcatgcgca tcttccctga agatgcacac tgatttcatt
     7261 gattttcact tgtatcttga tttgggcgga tacagtttgg tctggagttt taatcatcat
     7321 cggacagttc cgggtcccat ccattttccc gggcgcgttt tagtgcatct tcctgaattc
     7381 ttaggtactt ttctttaagc tcgggtggca agcggctcgg gatgtgtgca tacggttccc
     7441 aggcagcaaa caccttttca tacagcattt gaatctgtgt gtgggtccag ccggtacccg
     7501 tttccgtttc tggaataaag ccaaacagcc aaccatctcg gataattttt ccgatgtgcg
     7561 tcatgccgcc ttgtgcgtca agatcaagac cgacatagct ggtgtgtttc atgttgtgcg
     7621 tattacctgt tggtacggaa ggcttaaaag aacgttttga cgacggtgtc gagtgcatcg
     7681 cgcatgtcga catcatctgt gatcgggttg acaagcgtca tggtgcaggc agcgcgggca
     7741 tcaacgcctt tggcaatcaa tttgcctgca tagactaaca gacgcgtgga gaggccttca
     7801 tccaggccat gacccttgag gttacgtgct ttttgtgcga tctgaaccag tttttcagca
     7861 gtgtctttat caaccccggc ctcgtggctc acaatttcgg cttcaatgtc tggtttaggg
     7921 tagtcaaaat caagcgcacc aaaacgttgc ttggtggatt gcttgaggtc tttcatcagg
     7981 ctttgatagc ccggattgta agaaatcacg atttggaaat cgggatgcgc ttcaaccaat
     8041 tcgccctttt tctcaagtgg cagtactcga cggtgatcgg tcaatggatg gatgacgacg
     8101 atggtatcct gacgggcctc aacaatttca tcgaggtagc agatggcacc aatgcgcgca
     8161 gcgaccgtaa gcgggccatc ttgccaacgg gtgccatctt tatcgagcaa atagcggccg
     8221 acaagatcgg atgccgtcat gtcttcatta cacgcgatgg tgattaaggg gcggttgagc
     8281 ttccaagcca tatgttcgac aaaacgtgat ttaccacagc cggtcgggcc tttgagcatc
     8341 atgggaatgc gtgcttcgta accggcgcga tataactcga tttcgtcttg tacagctcga
     8401 taatagggct ctttgcgtac gcgatattga tctgcatttt gtgtcattta aattgccttg
     8461 ttctggtcga tgtagccgtc tttctgattt aaatcgtatt tatttagcgt ctgttttgaa
     8521 cttgaccgga cgactgacct gaatgtcaga aacaaatact actccgctgt gtttgctgaa
     8581 aaagggggcg aagccttcca gcaagggaga aacgagctct tccggaacgg cggtgatgat
     8641 catgttcagc acttcatctt cgttaaacat gagatggcct tcatgaaagc cttgagcccc
     8701 tttgcctgaa aggttgccga caatggtgta gcccttgacg cctgctcggt cgagtaaatc
     8761 cgtggcgaat gattgatgtt caccttcgag gatgatttcc agttttttca attgggtaag
     8821 gttgaggttt ttcatgcgct gagttcttct tcaagcaagg gtttgaccga gggcgtgtac
     8881 tgcggatcat tgttgagatt gtaccattcg ccgcgatcat aaaaaagacg atcgcccgtt
     8941 tccggatcaa cgacaacaac agtgatccag ccgttggtaa tcagggacat caccttgggc
     9001 aatcgacccg ctacttcgag ggtgaatgct gcgggtgctt cgataatcgc caagagacgg
     9061 atgggctcgt ggtatgggcg tccatcttta agcaccgact gtgctggtaa ccctgttcgc
     9121 aagtcactta aattacccgt aaccacacca aaacgaccta cgacgttgtg ataggccttg
     9181 ctgccactgc caaagtgttc gttatctacc gctgagaaat aatgctcaag attgatccac
     9241 tggccgataa ttagcgggtt gctgagaatg ttttcaaggt ggcggccttt gggatcgagt
     9301 cgataatcgt aagactgcaa aaacgttcga ccgcttaagt tcgcaccctc ggtcaaatgt
     9361 cggccgccgg cgacgacgct cgcatttctc gccaagcccc actctggccg aacttgggtc
     9421 cagtcgctgg cattccgctc aatttgtttt tgtgccgata atgtgtcgat gttgggcgaa
     9481 ggatgatcaa gcagagcagg caagcgttct gccgctgaca aacgggtggc cgcacgtaag
     9541 ccgttacgca agcgttcgag gtaaacaaga tggctgtttg gaagcaaatc aaggtcttgc
     9601 agtgacaatt gatcggttgt ggtgttgtgc atgacggaca caaaccaagt atcttcaggt
     9661 atctggatgc cttgcttggc cagtctgcga cgtacttcag gcttattggc catgtgggca
     9721 agcacccggg cagaaaccag cccatgacta ccaccacatg caccgcagtc aagcgcggat
     9781 tcgtagggat tattgtcgga agtgctgccg tgaccggcca ataaaacaaa gcgtgaaaag
     9841 ttttcggtca atccgatgga tgtgagtgcc ttgtcaacat aaaacacctg ttcatcgagg
     9901 ctaaaaccaa tcctggccaa gcgttccatt tgcatggaaa caaatgagcg attgatttta
     9961 tagtcctttt tcagccctgc gataaactga gtttcggccg ttgggctcaa ggcgaaacgg
    10021 cgcgcgaatt cggtctgttc gtgatagtta tccatggccg cttcgcgcag ttcgcggatc
    10081 atggcatcgg tgactgcttc gcgttcgatg ccaaattcct gatgaatggc gcgcacaatc
    10141 atggcgcgtt gcagcgaacg gacgattgag tcggcttgtt cgcgggtgag tttatccacc
    10201 agcaaacgag ttgcctgtgc ttttggttcg atatgattgc gtatttgggt gtagcgtcgc
    10261 ggggcaatgg ttttgccgat catgtcgaaa ccaaacagca aaccggccgc ttcaaccgtg
    10321 aagtagggtg aaaggatcga gcttttcatc tcatgtagca catggtcggc ggaagaataa
    10381 aagtctgttt caatgcttgt ggcacccacg ggcacttcaa gcaccagatt tttaggggta
    10441 atgaccaccg ggcaaagatt aacttcactg cccttgccaa ggccaatata gcttaccgga
    10501 acaccaaaaa acccggcgat accataggtt tgatactcgc ctaccgtttc cagattacgg
    10561 cgtatcggct cggagcgcac atcgatacag aacaatgcct gggcaaaggg ccgtttttct
    10621 tttttatgcg gtgcatacag ttgaatctgg ttgatgattt cccgtcgata gaccgattcc
    10681 atcgcacgaa gccagatcat gccttcttcg ttttcaaacg cctcgataag tgtcatgagt
    10741 tgcttgattt gaggcgcatt cagcgccata agggcatcgg tttgccccgc tttgctggct
    10801 aaatcacgaa gcgcatcagc ttgtcgtgtt gcctcgaatt ggcgttgttg gcgcaggtag
    10861 cccggaagaa tgttgttgat cctttgaggc tttttatgcg acagcgcatc atcaacatca
    10921 tgagcaaacg cgggcaatat gcagccaccg taataggctt gacgcagata gcattcggcg
    10981 gtgtgccctt cgatatattc ttgcagcact ttgactgtca tcggtgtgcg cttttgacgg
    11041 ctatgttcac ggatcaaggc caagcctaaa acgagccgga tggccaggaa atcgatgaga
    11101 tcccccgggt actgctcggc ccaatagtag tgcttggaag aggagcgcca gcgaataaaa
    11161 cctgcccagc catgcaaacg agtcagttcg cgggtaatca gcccatccca gtgagcctcc
    11221 tcaatgccca gttcgtcgag aatgtaggcg atgatgccct ctggcgtatc gtcctgatcg
    11281 aggatctgtt tgatatgcat gccacgtaaa aacaggcgta aattacgttt tgccagggcc
    11341 gtccaagctg cgaacaatcc ttggtggcgg ccgggcattt ggatggttga ttggccttca
    11401 tcgaaaaagt cgagacagct tttgattacc aattcatcga gtgtggtgcc catttgcgtg
    11461 ccgaacagca aatcgatggc ttcgtacagg gggcgctctg gtgcaaactg cgcacataag
    11521 cgttgagtta gcgctttaag atcgagcgcc tctgcatttt ccagttgttt cccgtgcaag
    11581 gcttggaaca catcatcatg atcggcgagc gcgcgcgttc tagttacttt gttcttaacc
    11641 gaacacatca agtcgctaag taacgatgcc aaatccaaac cgttgagcgt ttcttgtttt
    11701 gaaataaaat cggctatgtc acgctttatg ctgttttgat caactcggcc ttccttgtga
    11761 tagcgctggt aatcctcacg cggcaaaaat ccccgtgcgt gaaagagttt ttcaccctgg
    11821 cgaaccgcct gttcgaaggg cagatgctcc agaccatgga gcgggttatg gtgaatgaac
    11881 gtccgcatag gccagaaatt ggggattggc tcggcagcca tgtgcaccat ggaacgaatt
    11941 ttcaggcgtt tacccaaagg gagtttgctc atgggatcaa gctcctgtcc aaatcagatt
    12001 aatcacaaca atcgttgcta tcaggccaac aaagccccat gttgtggttg aagagagatc
    12061 ggcggcatct gtctcggcgc gtggcccgaa aacaaaccca ttcaataacc gcgtgcctgc
    12121 ccaactccaa agcaaccagg taagcaaaat cggcagagcg aataagatgg agctatgcgc
    12181 cagcaatgcc aacattgcaa aaaaactggg gaaaataggg agtgccattg cggccaacac
    12241 cacgatgaca atgacgctgg ataagcgcgg taaagcccgc gccaacccca caactaaacc
    12301 actgtaagca gcgccaaaac gttcttgcag tgcaatggca atgaagatca gcaaagccgg
    12361 cgcaacggac atccacaggg cgaaactgtg cagttcgcta ctggctggaa gatcggcggc
    12421 gataatccaa gccagcgccc aggcggacgt ggcgagcatc ccggcccact gggagagcac
    12481 acgcaccgtc agcaatcgcc aggcatagaa gactgccgta agcactgccc agccaacgaa
    12541 gaatccggaa tccagcggca aaccagccca tgcaagcaag aaaacgccga tttgaggcca
    12601 gataaaaatc agcagtaccc gcgcgatggg gtttttgatt cggccgagca gggcattgaa
    12661 taccatgctc aaggggaaca agggcagaaa gaacccggcc agtaacatca agaaaaagga
    12721 catatcaata ccacctcatc cagacattga agcgtttgga tgcatcaagc agccagtgcg
    12781 ccatccggtc gtagagatcg gctacataaa attcacgcga gatcagtgag tagagagtga
    12841 ggcgaaccgc tccgaaccgg tcaccgaaaa ccgatttctc acgactggcc aaatagctgc
    12901 taaaccagcc tgccaccaca atcaaagcga gcaagaaaac aagtccgtcg aacgtcagtt
    12961 tatcgatgcc tgctgcatgg tacagcgcat tacggaaggc ttcttcgggg taaaggaagt
    13021 tttcgaaagc atgaccaatg aaggtgtagc ccaccacaat caaggtaaac gacaataaaa
    13081 tcatcatcat catgcgaatt gggttattgg catccagatg atgtgtggca aaaagaacct
    13141 gaacccccgt aatccagccg aagaacagca gcacgatggc accttgtttt tcgaaaaaat
    13201 ccggtgccac gaaccagtgt gcaataacca gaatgaacag aggtacgacc agcgtagcca
    13261 agccgataaa taaccaaggc aattttaatg atgcagactt tcgttcaacc aagaaggtgt
    13321 gcagagggtt atgtggcacg ccgtcgtgct tgcgtgcttc atgaatcatg ctgcccgaac
    13381 cgaggaacat ggtgcctttg aacagaccat gcgcaatgag atggaacacg gccagagaaa
    13441 aagcgccaag accacactcc atgaccatga agcccatttg acccatggtg gaataaccca
    13501 gtgatttttt gatgtcgttt tgcgtcaaca tcagcaccga tcccaccagc gcggtaatca
    13561 gacccacgac aaacagcatg tgcagtacgc catcactgtg aacaaaaaca ggcgcgaagc
    13621 ggttgatgat aaaaccaccg gcgttgacaa tccccgcatg catcaaagct gaaaccggcg
    13681 tcgggccctc catggtatag gggagccagg tatgtagcgg gaattgggcg gattttgcaa
    13741 aggcagagag cgccaccaaa aaagcagccg ttatcgccgt gggcagcccc atgatggtgg
    13801 cgtttggatc ggccgcgata cgggaaaata aggtggggaa gtcaatcgca ccataggttt
    13861 gataaagcag cacggcggcg agcaccaatg gcaagtcccc caaccggtac gtgataaacg
    13921 tccaaaaggc gtagcgctgg ctcggccatg attctgtatt ctgacccaaa agaaagtaga
    13981 gcatgatgcc gatcatgtgc caggcgatca gcaatgtgac caagtcaccg gcggcgacca
    14041 taagcaaaat ggaagcggtc atcaaatcca gcagcaagaa gaaccgggga tagcccggtt
    14101 cttcggtcat gtagcggatt gaatagacat gaacaatcag gctgatgccc gctacaacca
    14161 gcgacatgat gctgctcagc ggatcgacgt acagactgcc ccacatcgtg cccagagaat
    14221 gccagcttgg gctcgcatcg gtaccgatat acgctacgag ctgatatgcg gcaacaatga
    14281 cggtcagtgt tcccaacgcc acgctcaggt gggcaacacg tttaccgagt cgagcggcaa
    14341 acaattgcac cagtagggcg gacagtaagg gtaacaatgg aattaaccat tgcaggttca
    14401 tcatgcgctt tctccatcac agaccggcct tggccgggta gttatcttgc gtatacgcaa
    14461 attaaaatta atcgggtttc aggcgcattg tcctcgtatc ccgagcagca atttgaacag
    14521 cttcatgaga tttgtttctt gtctgaagct cggacggaaa gttctatcgc gtcatcgaca
    14581 aataa
//
