LOCUS       sequence-341133-        9347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Wong JM, Kusdra L, Collins K
  TITLE     Subnuclear shuttling of human telomerase induced by transformation 
            and DNA damage.
  JOURNAL   Nat Cell Biol. 2002 Sep . 4(9):731-6.
  PUBMED    12198499
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Sep . 4(9):731-6."
COMMENT     Sequence Label: pBABEhygroHigheGFPhTERT
FEATURES             Location/Qualifiers
     source          1..9347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1389..1398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1395..1397,1398..1400,1401..2111)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1395..1397,1398..1400,1401..2111)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1440..1461)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2048..2069
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2127..5525
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(5537..5557)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5542..5871
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5722..5857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5945..6970
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5945..6970
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     7487..7506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             7768..8237
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(8352..9143,9144..9212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8975..8994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9213..9317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcgctgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1441 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1501 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1561 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1681 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1741 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1801 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1861 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1921 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1981 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     2041 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2101 agctgtacaa gggagcaggt gctcatatgc cgcgcgctcc ccgctgccga gccgtgcgct
     2161 ccctgctgcg cagccactac cgcgaggtgc tgccgctggc cacgttcgtg cggcgcctgg
     2221 ggccccaggg ctggcggctg gtgcagcgcg gggacccggc ggctttccgc gcgctggtgg
     2281 cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc ccctccttcc
     2341 gccaggtgtc ctgcctgaag gagctggtgg cccgagtgct gcagaggctg tgcgagcgcg
     2401 gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc gggggccccc
     2461 ccgaggcctt caccaccagc gtgcgcagct acctgcccaa cacggtgacc gacgcactgc
     2521 gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg ctggttcacc
     2581 tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac caggtgtgcg
     2641 ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca cacgctagtg
     2701 gaccccgaag gcgtctggga tgcgaacggg cctggaacca tagcgtcagg gaggccgggg
     2761 tccccctggg cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc agccgaagtc
     2821 tgccgttgcc caagaggccc aggcgtggcg ctgcccctga gccggagcgg acgcccgttg
     2881 ggcaggggtc ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt ggtttctgtg
     2941 tggtgtcacc tgccagaccc gccgaagaag ccacctcttt ggagggtgcg ctctctggca
     3001 cgcgccactc ccacccatcc gtgggccgcc agcaccacgc gggcccccca tccacatcgc
     3061 ggccaccacg tccctgggac acgccttgtc ccccggtgta cgccgagacc aagcacttcc
     3121 tctactcctc aggcgacaag gagcagctgc ggccctcctt cctactcagc tctctgaggc
     3181 ccagcctgac tggcgctcgg aggctcgtgg agaccatctt tctgggttcc aggccctgga
     3241 tgccagggac tccccgcagg ttgccccgcc tgccccagcg ctactggcaa atgcggcccc
     3301 tgtttctgga gctgcttggg aaccacgcgc agtgccccta cggggtgctc ctcaagacgc
     3361 actgcccgct gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg gagaagcccc
     3421 agggctctgt ggcggccccc gaggaggagg acacagaccc ccgtcgcctg gtgcagctgc
     3481 tccgccagca cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc ctgcgccggc
     3541 tggtgccccc aggcctctgg ggctccaggc acaacgaacg ccgcttcctc aggaacacca
     3601 agaagttcat ctccctgggg aagcatgcca agctctcgct gcaggagctg acgtggaaga
     3661 tgagcgtgcg gggctgcgct tggctgcgca ggagcccagg ggttggctgt gttccggccg
     3721 cagagcaccg tctgcgtgag gagatcctgg ccaagttcct gcactggctg atgagtgtgt
     3781 acgtcgtcga gctgctcagg tctttctttt atgtcacgga gaccacgttt caaaagaaca
     3841 ggctcttttt ctaccggaag agtgtctgga gcaagttgca aagcattgga atcagacagc
     3901 acttgaagag ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag catcgggaag
     3961 ccaggcccgc cctgctgacg tccagactcc gcttcatccc caagcctgac gggctgcggc
     4021 cgattgtgaa catggactac gtcgtgggag ccagaacgtt ccgcagagaa aagagggccg
     4081 agcgtctcac ctcgagggtg aaggcactgt tcagcgtgct caactacgag cgggcgcggc
     4141 gccccggcct cctgggcgcc tctgtgctgg gcctggacga tatccacagg gcctggcgca
     4201 ccttcgtgct gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt gtcaaggtgg
     4261 atgtgacggg cgcgtacgac accatccccc aggacaggct cacggaggtc atcgccagca
     4321 tcatcaaacc ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag aaggccgccc
     4381 atgggcacgt ccgcaaggcc ttcaagagcc acgtctctac cttgacagac ctccagccgt
     4441 acatgcgaca gttcgtggct cacctgcagg agaccagccc gctgagggat gccgtcgtca
     4501 tcgagcagag ctcctccctg aatgaggcca gcagtggcct cttcgacgtc ttcctacgct
     4561 tcatgtgcca ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc caggggatcc
     4621 cgcagggctc catcctctcc acgctgctct gcagcctgtg ctacggcgac atggagaaca
     4681 agctgtttgc ggggattcgg cgggacgggc tgctcctgcg tttggtggat gatttcttgt
     4741 tggtgacacc tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc cgaggtgtcc
     4801 ctgagtatgg ctgcgtggtg aacttgcgga agacagtggt gaacttccct gtagaagacg
     4861 aggccctggg tggcacggct tttgttcaga tgccggccca cggcctattc ccctggtgcg
     4921 gcctgctgct ggatacccgg accctggagg tgcagagcga ctactccagc tatgcccgga
     4981 cctccatcag agccagtctc accttcaacc gcggcttcaa ggctgggagg aacatgcgtc
     5041 gcaaactctt tggggtcttg cggctgaagt gtcacagcct gtttctggat ttgcaggtga
     5101 acagcctcca gacggtgtgc accaacatct acaagatcct cctgctgcag gcgtacaggt
     5161 ttcacgcatg tgtgctgcag ctcccatttc atcagcaagt ttggaagaac cccacatttt
     5221 tcctgcgcgt catctctgac acggcctccc tctgctactc catcctgaaa gccaagaacg
     5281 cagggatgtc gctgggggcc aagggcgccg ccggccctct gccctccgag gccgtgcagt
     5341 ggctgtgcca ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc tacgtgccac
     5401 tcctggggtc actcaggaca gcccagacgc agctgagtcg gaagctcccg gggacgacgc
     5461 tgactgccct ggaggccgca gccaacccgg cactgccctc agacttcaag accatcctgg
     5521 actgagtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5581 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5701 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5821 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgcc
     5881 agctggggcg ccctctggta aggttgggaa gccctgcaga tctgatcccg gggggcaatg
     5941 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     6001 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     6061 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     6121 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     6181 cattggggaa tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     6241 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     6301 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     6361 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     6421 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     6481 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     6541 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     6601 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     6661 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     6721 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     6781 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     6841 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     6901 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     6961 aaaggaatag agtagatgcc gaccgggatc tatcgatgcg gcgggtgtgg tggttacgcg
     7021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc atcgataaaa taaaagattt
     7621 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7681 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
     7741 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     7801 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
     7861 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     7921 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     7981 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     8041 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
     8101 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     8161 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     8221 tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt
     8281 atttacatta aatggccata gtgcggccgc aaatcaatct aaagtatata tgagtaaact
     8341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcagc
//
