LOCUS       sequence-341140-        8005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 
            marker and opto-control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Kakumoto T, Nakata T
  TITLE     Optogenetic control of PIP3: PIP3 is sufficient to induce the 
            actin-based active part of growth cones and is regulated via 
            endocytosis.
  JOURNAL   PLoS One. 2013 Aug 7;8(8):e70861. doi: 10.1371/journal.pone.0070861.
            eCollection 2013.
  PUBMED    23951027
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-7"; volume: "8(8):e70861. doi"; pages: " 
            10.1371/journal.pone.0070861. eCollection 2013"
COMMENT     Sequence Label: AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
FEATURES             Location/Qualifiers
     source          1..8005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     556..578
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     640..659
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1248..1257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1254..1961
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1399..1417)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1667..1686
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1962..2015
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2028..3521
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3522..3575
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3588..4097
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     polyA_signal    4281..4402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4318..4337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4372..4391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4409..4864)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4546..4567)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4758..4777
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4891..4995
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4997..5354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4997..5188
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5020..5040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5205..5340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5389..6183
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5389..6183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5443..5462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6053..6072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6371..6390)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6415..6462
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6791..7379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7280..7299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7505..7884
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
       61 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
      121 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
      181 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
      241 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
      301 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
      361 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
      421 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
      481 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
      541 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
      601 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
      661 tgtctcatca ttttggcaaa gaattgattt atcgctagcg ctaccggact cagatctcga
      721 gctcaagctt cgaattcgcc accatgagcg acgtggctat tgtgaaggag ggttggctgc
      781 acaaacgagg ggagtacatc aagacctggc ggccacgcta cttcctcctc aagaatgatg
      841 gcaccttcat tggctacaag gagcgtccgc aggatgtgga ccaacgtgag gctcccctca
      901 acaacttctc tgtggcgcag tgccagctga tgaagacgga gcggccccgg cccaacacct
      961 tcatcatccg ctgcctgcag tggaccactg tcatcgaacg caccttccat gtggagactc
     1021 ctgaggagcg ggaggagtgg acaaccgcca tccagactgt ggctgacggc ctcaagaagc
     1081 aggaggagga ggagatggac ttccggtcgg gctcacccag tgacaactca ggggctgaag
     1141 agatggaggt gtccctggcc aagcccaagc accgcgtgac catgaacgag tttgagtacc
     1201 tgaagctgct gggcaagggc actttcggca aggtggatcc accggtcgcc accatggtga
     1261 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     1321 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1381 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     1441 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     1501 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     1561 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     1621 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1681 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     1741 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     1801 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     1861 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1921 agggccgcca ctccaccggc ggcatggacg agctgtacaa ggagggcagg ggaagtcttc
     1981 taacatgcgg ggacgtggag gaaaatcccg gccccgcacc gggtaccatg aagatggaca
     2041 aaaagactat agtttggttt agaagagacc taaggattga ggataatcct gcattagcag
     2101 cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac
     2161 agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct cacttatctc
     2221 aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac acgatttcag
     2281 cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac cacctctatg
     2341 atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa cgtgggatct
     2401 ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac tgcgaaaagg
     2461 gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg tcgattgaat
     2521 ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct gaagcgattt
     2581 gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt
     2641 tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta aatgagttca
     2701 tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg aattctactt
     2761 cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt ttccagtgtg
     2821 cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa gaaagtgcag
     2881 atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt ttcaacttcc
     2941 cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg gatgctgatg
     3001 ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg gatgccggaa
     3061 tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt gtttcaagct
     3121 ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc tgggatacac
     3181 ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct gggagtatcc
     3241 ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc
     3301 cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca actgaatgga
     3361 tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg gaactcggaa
     3421 caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta gctaaagcta
     3481 tttcaagaac ccgtgaagca cagatcatga tcggagcagc agagggccgc ggaagtcttc
     3541 taacatgcgg ggacgtggag gaaaatcccg gccccgcacc gggatccatg aatggagcta
     3601 taggaggtga ccttttgctc aattttcctg acatgtcggt cctagagcgc caaagggctc
     3661 acctcaagta cctcaatccc acctttgatt ctcctctcgc cggcttcttt gccgattctt
     3721 caatgattac cggcggcgag atggacagct atctttcgac tgccggtttg aatcttccga
     3781 tgatgtacgg tgagacgacg gtggaaggtg attcaagact ctcaatttcg ccggaaacga
     3841 cgcttgggac tggaaatttc aaggcagcga agtttgatac agagactaag gattgtaatg
     3901 aggcggcgaa gaagatgacg atgaacagag atgacctagt agaagaagga gaagaagaga
     3961 agtcgaaaat aacagagcaa aacaatggga gcacaaaaag catcaagaag atgaaacaca
     4021 aagccaagaa agaagagaac aatttctcta atgattcatc taaagtgacg aaggaattgg
     4081 agaaaacgga ttatattcat gtactgtata agggtaaaaa gaagaaaaag aagtcaaaga
     4141 caaagtgtgt aattatgtag cggccgcgac tctagatcat aatcagccat accacatttg
     4201 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     4261 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     4321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4381 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     4441 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4501 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4561 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4621 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4681 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4741 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4801 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4861 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4981 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     5041 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5101 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5161 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5221 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5281 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5341 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5401 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5461 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5521 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     5581 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5641 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5701 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5761 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5821 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5881 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     5941 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6001 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6061 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6121 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6181 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6241 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6301 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     6361 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     6421 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     6481 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     6541 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     6601 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     6661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     7501 tatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7561 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7621 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7681 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7741 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7801 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7861 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     7921 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     7981 gcgatggggg cggggggggg ggggg
//
