LOCUS       sequence-341193-       11017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Hughes H, Stephens DJ
  TITLE     Sec16A defines the site for vesicle budding from the endoplasmic 
            reticulum on exit from mitosis.
  JOURNAL   J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2.
  PUBMED    21045114
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-12-1"; volume: "123(Pt 23)"; pages: "4032-8. Epub
            2010 Nov 2"
COMMENT     Sequence Label: pLVX-Puro-mRuby-Sec23A
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2821..2830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5852..6351
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5887..5910)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6284..6303
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6372..6971
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6372..6391)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6868..6888
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6985..7573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7038..7058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7456..7467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7780..8413
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8542..8558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8542..8558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8555..8577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8566..8582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8590..8596,8597..8614,8615..8620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8635..8656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8773..8790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8944..9532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9024..9043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9703..10494,10495..10563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10326..10345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10564..10668)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10716..10850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10753..10772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10807..10826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgac
     2821 gccaccatgg cctcctctga agacgtgatc aaagagttca tgcgcttcaa ggtcaaaatg
     2881 gaggggtcag tgaatgggca cgagtttgag atagaaggcg aaggagaagg aagaccctat
     2941 gagggtaccc aaacagcgaa gctcaaagtc accaagggcg gaccattacc ctttagctgg
     3001 gacatcctca gcccacagtt tcagtatggc tcaaaagcct acgtgaaaca ccctgcagac
     3061 ataccggact atctgaaact gagctttccc gaagggttca aatgggagag gttcatgaac
     3121 ttcgaagatg gcggcgttgt tactgtcact caagattcca cacttcagga tggagagttc
     3181 atctacaagg tgaaattgag agggacgaac tttcccagtg atggccctgt aatgcagaag
     3241 aaaaccatgg gttgggaagc cagtacagaa cggatgtatc cagaggatgg tgctctgaaa
     3301 ggggagatca agatgcgact aaagctgaag gacggtgggc attacgatgc cgaggtgaag
     3361 acaacctaca aggccaagaa gcaggtacag cttcctggag cttacatgac ggacattaag
     3421 ctggatatca tttcccacaa tggcgactac accattgtgg agcagtatga gcgtgcagaa
     3481 ggcaggcatt ctactggagc tctcgaggaa ttctgcagtc gacggtaccg cgggcccatg
     3541 acaacctatt tggaattcat tcaacaaaat gaagaacgag atggagtccg atttagttgg
     3601 aatgtttggc catcaagtcg actggaagct acaagaatgg ttgttcctgt ggcagccctg
     3661 tttacaccac tgaaagagag acctgactta ccacctattc aatatgaacc tgttctgtgt
     3721 agtaggacca cttgccgtgc agttttgaat cctttatgtc aagtggatta tcgagcaaaa
     3781 ctttgggctt gcaacttttg ttaccaaagg aatcagtttc cacctagtta tgctggtata
     3841 tctgaactga atcagcctgc tgaactttta cctcagtttt ctagcattga atatgtagtt
     3901 ctgcgtggtc ctcagatgcc tttgatattc ctctatgtgg ttgatacttg catggaagat
     3961 gaagatttac aagccctgaa agaatccatg cagatgtcat taagtctttt accacctaca
     4021 gctttggttg gacttattac ttttgggaga atggttcagg ttcatgaact tggatgtgaa
     4081 ggcatttcaa aaagctatgt cttcagagga acaaaagatt tgtctgccaa acaactgcag
     4141 gaaatgctgg ggctctctaa agtaccagtt actcaagcaa cacgtggtcc tcaggtacag
     4201 cagccacctc cttccaacag attcttacaa ccagtacaga aaatagacat gaatctcaca
     4261 gatcttctgg gagaactcca gcgagaccct tggcctgtac cacagggaaa gagacctttg
     4321 cgttcctctg gggtggcact ttccatagct gtaggactgc tggagtgtac ttttcccaac
     4381 actggtgctc gtatcatgat gttcattggt ggtcctgcta ctcaggggcc tggaatggtg
     4441 gttggagatg agttgaagac acctataaga tcgtggcatg acattgacaa agacaatgcc
     4501 aaatatgtta aaaagggaac taagcatttt gaagcattgg ctaatcgagc tgctacaact
     4561 ggccatgtta ttgatatcta tgcgtgtgca ttagatcaga caggtctcct ggagatgaaa
     4621 tgctgtccca accttactgg aggatacatg gtaatgggtg attctttcaa tacttcctta
     4681 ttcaaacaaa cttttcaaag agtctttacc aaagacatgc atggacagtt taaaatgggc
     4741 tttggtggta cgctagaaat aaagacctca agggaaataa agatttcagg agctattgga
     4801 ccctgtgtgt cactcaattc taaaggaccc tgtgtgtctg aaaatgagat aggaacaggt
     4861 ggcacatgtc agtggaagat atgtggactt agtcccacta caaccttagc catatatttt
     4921 gaggttgtca atcagcataa tgctccaatt cctcaaggag ggcgtggtgc aatccagttt
     4981 gtgactcagt atcagcattc aagtgggcag agacgcatcc gagtgaccac cattgctagg
     5041 aactgggcag atgctcaaac tcaaatccaa aacattgctg catcttttga ccaggaggca
     5101 gctgccattc ttatggcccg gctagcaata tatagagcag aaacagaaga aggtccagat
     5161 gtgcttaggt ggctggacag acagctcatt cgactgtgtc agaaatttgg agaatatcat
     5221 aaagatgacc caagttcctt cagattttca gaaactttct ccctttatcc acagtttatg
     5281 tttcatttaa gaagatcttc tttcctgcaa gtttttaaca atagtcctga tgagagttca
     5341 tattatcgtc accattttat gcgtcaagat ctgacccagt ctctaattat gattcagcct
     5401 atcctgtatg cgtattcttt tagtggacca ccagagccgg ttcttcttga tagcagtagc
     5461 attcttgcag atcgtattct tctcatggac acattcttcc agattttgat ttatcatggt
     5521 gagaccatag cacagtggcg gaagtcagga taccaggata tgcctgagta tgaaaatttc
     5581 cgccaccttc tgcaagcccc agtggatgat gcacaggaaa ttcttcactc cagatttcca
     5641 atgccaagat acattgacac tgaacatgga ggcagccagg cccgtttcct cctttcaaaa
     5701 gtcaaccctt cacagactca taataatatg tatgcctggg ggcaggagtc tggagcacct
     5761 attcttacag atgatgttag tttacaagtg tttatggatc acttgaagaa acttgctgtg
     5821 tccagtgctg cttaatctag ataattctac cgggtagggg aggcgctttt cccaaggcag
     5881 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     5941 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6001 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6061 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6121 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6181 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     6241 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     6301 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     6361 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6421 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6481 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6541 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6601 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6661 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6721 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6781 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6841 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6901 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6961 ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     7021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7381 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7561 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     7621 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7681 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7741 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7801 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7861 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7921 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7981 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     8041 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     8101 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     8161 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8221 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8401 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8461 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
     8521 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8581 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8641 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8701 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8761 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8821 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8941 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9001 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9061 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9121 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9181 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9241 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9301 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9361 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9421 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9481 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10681 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
    10741 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10801 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10861 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10921 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10981 attgtatttg ttaaatatgt actacaaact tagtagt
//
