LOCUS       sequence-341380-        7757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector to express sgRNA resistant human ACSL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, 
            Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang 
            HC, Min W, Birsoy K
  TITLE     CHP1 Regulates Compartmentalized Glycerolipid Synthesis by 
            Activating GPAT4.
  JOURNAL   Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi: 
            10.1016/j.molcel.2019.01.037. Epub 2019 Mar 4.
  PUBMED    30846317
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.01.037"; journalName: "Mol Cell"; date: 
            "2019-04-4- 4"; volume: "74"; issue: "1"; pages: "45-58.e7"
COMMENT     Sequence Label: pMXS-IRES-Blast ACSL3
FEATURES             Location/Qualifiers
     source          1..7757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(4110..4683,4684..4686,4687..4695)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4276..4293)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4503..4522
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4696..5091
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5262..5731
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5861..5878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6032..6620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6112..6131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6784..7575,7576..7644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7407..7426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7645..7749)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaagcca
     1921 ccatgaataa ccacgtgtct tcaaaaccat ctaccatgaa gctaaaacat accatcaacc
     1981 ctattctttt atattttata cattttctaa tatcacttta tactatttta acatacattc
     2041 cgttttattt tttctccgag tcaagacaag aaaaatcaaa ccgaattaaa gcaaagcctg
     2101 taaattcaaa acctgattct gcatacagat ctgttaatag tttggatggt ttggcttcag
     2161 tattataccc tggatgtgat actttagata aagtttttac atatgcaaaa aacaaattta
     2221 agaacaaaag actcttggga acacgtgaag ttttaaatga ggaagatgaa gtacaaccaa
     2281 atggaaaaat ttttaaaaag gttattcttg gacagtataa ttggctttcc tatgaagatg
     2341 tctttgttcg agcctttaat tttggaaatg gattacagat gttgggtcag aaaccaaaga
     2401 ccaacatcgc catcttctgt gagaccaggg ccgagtggat gatagctgca caggcgtgtt
     2461 ttatgtataa ttttcagctt gttacattat atgccactct aggaggtcca gccattgttc
     2521 atgcattaaa tgaaacagag gtgaccaaca tcattactag taaagaactc ttacaaacaa
     2581 agttgaagga tatagtttct ttggtcccac gcctgcggca catcatcact gttgatggaa
     2641 agccaccgac ctggtccgag ttccccaagg gcatcattgt gcataccatg gctgcagtgg
     2701 aggccctggg agccaaggcc agcatggaaa accaacctca tagcaaacca ttgccctcag
     2761 atattgcagt aatcatgtac acaagtggat ccacaggact tccaaaggga gtcatgatct
     2821 cacatagtaa cattattgct ggtataactg ggatggcaga aaggattcca gaactaggag
     2881 aggaagatgt ctacattgga tatttgcctc tggcccatgt tctagaatta agtgctgagc
     2941 ttgtctgtct ttctcacgga tgccgcattg gttactcttc accacagact ttagcagatc
     3001 agtcttcaaa aattaaaaaa ggaagcaaag gggatacatc catgttgaaa ccaacactga
     3061 tggcagcagt tccggaaatc atggatcgga tctacaaaaa tgtcatgaat aaagtcagtg
     3121 aaatgagtag ttttcaacgt aatctgttta ttctggccta taattacaaa atggaacaga
     3181 tttcaaaagg acgtaatact ccactgtgcg acagctttgt tttccggaaa gttcgaagct
     3241 tgctaggggg aaatattcgt ctcctgttgt gtggtggcgc tccactttct gcaaccacgc
     3301 agcgattcat gaacatctgt ttctgctgtc ctgttggtca gggatacggg ctcactgaat
     3361 ctgctggggc tggaacaatt tccgaagtgt gggattataa cacgggacgc gtgggagcac
     3421 cattagtttg ctgtgaaatc aaattaaaaa actgggagga aggtggatac tttaatactg
     3481 ataagccaca ccccaggggt gaaattctta ttgggggcca aagtgtgaca atggggtact
     3541 acaaaaatga agcaaaaaca aaagctgatt tctttgaaga tgaaaatgga caaaggtggc
     3601 tctgtactgg ggatattgga gagtttgaac ccgatggatg cttaaagatt attgatcgta
     3661 aaaaggacct tgtaaaacta caggcagggg aatatgtttc tcttgggaaa gtagaggcag
     3721 ctttgaagaa tcttccacta gtagataaca tttgtgcata tgcaaacagt tatcattctt
     3781 atgtcattgg atttgttgtg ccaaatcaaa aggaactaac tgaactagct cgaaagaaag
     3841 gacttaaagg gacttgggag gagctgtgta acagttgtga aatggaaaat gaggtactta
     3901 aagtgctttc cgaagctgct atttcagcaa gtctggaaaa gtttgaaatt ccagtaaaaa
     3961 ttcgtttgag tcctgaaccg tggacccctg aaactggtct ggtgacagat gccttcaagc
     4021 tgaaacgcaa agagcttaaa acacattacc aggcggacat tgagcgaatg tatggaagaa
     4081 aatgagctac gtaaattccg cccccccccc ccctctccct ccccccccct aacgttactg
     4141 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     4201 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     4261 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     4321 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     4381 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     4441 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     4501 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     4561 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     4621 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     4681 aatatggcca caaccatggt tcctttgtct caagaagaat ccaccctcat tgaaagagca
     4741 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     4801 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     4861 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     4921 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     4981 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     5041 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agtcgacgat
     5101 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     5161 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
     5221 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
     5281 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
     5341 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     5401 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
     5461 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     5521 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     5581 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
     5641 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
     5701 tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt
     5761 tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg
     5821 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5881 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5941 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6661 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6721 agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7381 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7741 ggttccgcgc acatttc
//
