LOCUS       sequence-341382-        7731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector to express sgRNA resistant human ACSL4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, 
            Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang 
            HC, Min W, Birsoy K
  TITLE     CHP1 Regulates Compartmentalized Glycerolipid Synthesis by 
            Activating GPAT4.
  JOURNAL   Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi: 
            10.1016/j.molcel.2019.01.037. Epub 2019 Mar 4.
  PUBMED    30846317
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.01.037"; journalName: "Mol Cell"; date: 
            "2019-04-4- 4"; volume: "74"; issue: "1"; pages: "45-58.e7"
COMMENT     Sequence Label: pMXS-IRES-Blast ACSL4
FEATURES             Location/Qualifiers
     source          1..7731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(4083..4657,4658..4660,4661..4669)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4250..4267)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4477..4496
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4670..5065
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5236..5705
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5835..5852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6006..6594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6086..6105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6758..7549,7550..7618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7381..7400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7619..7723)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaagcca
     1921 ccatgaaact taagctaaat gtgctcacca ttattttgct gcctgtccac ttgttaataa
     1981 caatatacag tgcccttata tttattccat ggtattttct taccaatgcc aagaagaaaa
     2041 acgctatggc aaagagaata aaagctaagc ccacttcaga caaacctgga agtccatatc
     2101 gctctgtcac acacttcgac tcactagctg taatagacat ccctggagca gatactctgg
     2161 ataaattatt tgaccatgct gtatccaagt ttgggaagaa ggacagcctt gggaccaggg
     2221 aaatcctaag tgaagaaaat gaaatgcagc caaatggaaa agtttttaag aagttaattc
     2281 ttgggaatta taaatggatg aactatcttg aagtgaatcg cagagtgaat aactttggta
     2341 gtggactcac tgcactggga ctaaaaccaa agaacaccat tgccatcttc tgtgagacca
     2401 gggccgaatg gatgattgca gcacagacct gctttaagta caactttcct cttgtgactt
     2461 tatatgccac acttggcaaa gaagcagtag ttcatgggct aaatgaatct gaggcttcct
     2521 atctgattac cagtgttgaa cttctggaaa gtaaacttaa gactgcattg ttagatatca
     2581 gttgtgttaa acatatcatt tatgtggaca ataaggctat caataaagca gagtaccctg
     2641 aaggatttga gattcacagc atgcaatcag tagaagagtt gggatctaac ccagaaaact
     2701 tgggcattcc tccaagtaga ccaacgcctt cagacatggc cattgttatg tatactagtg
     2761 gttctactgg ccgacctaag ggagtgatga tgcatcatag caatttgata gctggaatga
     2821 caggccagtg cgagaggatc cccggcctag gaccgaagga cacatatatt ggctacttgc
     2881 ctttggctca tgtgctagaa ctgacagcag agatatcttg ctttacctat ggctgcagga
     2941 ttggatattc ttctccgctt acactctctg accagtccag caaaattaaa aaaggaagca
     3001 aaggagactg tactgtactg aagcccacac ttatggctgc tgttccggaa atcatggata
     3061 gaatttataa gaatgttatg agcaaagtcc aagagatgaa ttatattcag aaaactctgt
     3121 tcaagatagg gtatgattac aaattggaac agatcaaaaa gggatatgat gcacctcttt
     3181 gcaatctgtt actgtttaaa aaggtcaagg ccctgctggg agggaatgtc cgcatgatgc
     3241 tgtctggagg ggccccgcta tctcctcaga cacaccgatt catgaatgtc tgcttctgct
     3301 gcccaattgg ccagggttat ggactgacag aatcatgtgg tgctgggaca gttactgaag
     3361 taactgacta tactactggc agagttggag cacctcttat ttgctgtgaa attaagctaa
     3421 aagactggca agaaggcggt tatacaatta acgataaacc gaatccaagg ggtgaaatcg
     3481 taattggtgg acagaacatc tccatgggat attttaaaaa tgaagagaaa acagcagaag
     3541 attattctgt ggatgaaaat ggacaaaggt ggttttgcac tggtgatatt ggagaattcc
     3601 atcccgatgg atgtttacag attatagatc gtaagaaaga tctagtgaag ttacaagcag
     3661 gagagtatgt atctcttggg aaagtagaag ctgcactgaa gaattgtcca cttattgaca
     3721 acatctgtgc ttttgccaaa agtgatcagt cctatgtgat cagttttgtg gttcctaacc
     3781 agaaaaggtt gacacttttg gcacaacaga aaggggtaga aggaacttgg gttgatatct
     3841 gcaataatcc tgctatggaa gctgaaatac tgaaagaaat tcgagaagct gcaaatgcca
     3901 tgaaattgga gcgatttgaa attccaatca aggttcgatt aagcccagag ccatggaccc
     3961 ctgaaactgg tttggtaact gatgctttca aactgaaaag gaaggagctg aggaaccatt
     4021 acctcaaaga cattgaacga atgtatgggg gcaaataagc tacgtaaatt ccgccccccc
     4081 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4141 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4201 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4261 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     4321 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     4381 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     4441 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     4501 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     4561 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     4621 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggttccttt
     4681 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     4741 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     4801 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     4861 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     4921 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     4981 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     5041 ctctggttat gtgtgggagg gctaagtcga cgataaaata aaagatttta tttagtctcc
     5101 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     5161 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     5221 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     5281 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     5341 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     5401 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     5461 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     5521 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     5581 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     5641 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     5701 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     5761 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5941 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6181 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6421 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     6721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt c
//
