LOCUS       sequence-341446-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with 
            flanking Nextera sequencing adapters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Pierce SE, Granja JM, Greenleaf WJ
  TITLE     High-throughput single-cell chromatin accessibility CRISPR screens 
            enable unbiased identification of regulatory networks in cancer.
  JOURNAL   Nat Commun. 2021 May 20;12(1):2969. doi: 10.1038/s41467-021-23213-w.
  PUBMED    34016988
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-20"; volume: "12(1):2969. doi"; pages: " 
            10.1038/s41467-021-23213-w"
COMMENT     Sequence Label: pSP618
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    382..400
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     404..424
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    632..650
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     promoter        682..893
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattgtac ctagtggaac
       61 cggaaccctt aaacatgtat aacttcgtat aatgtatgct atacgaagtt attaggtccc
      121 tcgacgttaa cccagtctga ctatcacgat ggtaatacgg ttatccacgc gggatccgac
      181 gcgccatctc taggcccgcg ccggccccct cgcacggact tgtgggagaa gctcggctac
      241 tcccctgccc cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc
      301 gataaaagac agataatctg ttctttttaa tactagctac attttacatg ataggcttgg
      361 atttctgtct cgtgggctcg gagatgtgta taagagacag aaacagcaca aaaggaaact
      421 caccctaact gtaaagtaat tgtgtgtttt gagactataa gtatcccttg gagaaccacc
      481 ttgttggcga acttaatccc gtggcaagtt taagagctaa gctggaaaca gcatagcaag
      541 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttgc
      601 tcgaatctac actcagcctc ggaatcagta tctgtctctt atacacatct gacgctgccg
      661 acgatgcatg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      721 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      781 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      841 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      901 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tgg
//
