LOCUS       sequence-34145-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1759-HQ858773
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(737..761)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatggc ggcttcgctg ggtctgaacc ctgaagctgt cttcacttcc
      181 tacacctcct cgccgccgtt catgtcggac tacgtggcgg cgagcttcct gccgccggcc
      241 gtcgtcgact ccacggactt ctctgcagag ctcgatgatc ttcaccacca cttggattac
      301 tcatcgccgg cgccgacctt ggccggggct cggagcgatc gcagcgagaa gcagatgatc
      361 aggtggtgtg agggtggtgg tggcgagaag agactcggta ggatcgggtt cagaacgaga
      421 tcagaggtgg agatcttgga cgatggattc aaatggagga agtatggcaa gaaggctgtc
      481 aagagtagcc caaatccaag gaactactac cgctgctcgt cggagggctg cggcgtgaag
      541 aagcgggtgg agagggaccg cgacgacccc cgctacgtca tcaccaccta cgacggcgtc
      601 cacaaccacg caagccccgc agccgccgcc atcatccagt acggcggcgg cggcggattc
      661 tatagcccgc cgcacagcgg ctcgccgtcg gccgcctcct actcgggctc cttcgtcctc
      721 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattat
//
