LOCUS       sequence-341477-        6308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of voltage sensor under the Syn promoter, 
            Cre-dependent expression; soma localization targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Abdelfattah AS, Zheng J, Singh A, Huang YC, Reep D, Tsegaye G, Tsang
            A, Arthur BJ, Rehorova M, Olson CVL, Shuai Y, Zhang L, Fu TM, Milkie
            DE, Moya MV, Weber TD, Lemire AL, Baker CA, Falco N, Zheng Q, Grimm 
            JB, Yip MC, Walpita D, Chase M, Campagnola L, Murphy GJ, Wong AM, 
            Forest CR, Mertz J, Economo MN, Turner GC, Koyama M, Lin BJ, Betzig 
            E, Novak O, Lavis LD, Svoboda K, Korff W, Chen TW, Schreiter ER, 
            Hasseman JP, Kolb I
  TITLE     Sensitivity optimization of a rhodopsin-based fluorescent voltage 
            indicator.
  JOURNAL   Neuron. 2023 Mar 28:S0896-6273(23)00205-2. doi: 
            10.1016/j.neuron.2023.03.009.
  PUBMED    37015225
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Mar 28:S0896-6273(23)00205-2. doi: 
            10.1016/j.neuron.2023.03.009."
COMMENT     Sequence Label: pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   45..174
                     /label=AAV2 ITR
     promoter        256..703
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    733..766
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    829..862
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1676..1685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2597..2606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2617..2650)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2721..2754)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2796..3384
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2849..2869)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3267..3278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3427..3548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3438..3457)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3492..3511
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3710..3839
                     /label=AAV2 ITR
     rep_origin      complement(4076..4664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4156..4175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4835..5626,5627..5695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5458..5477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5696..5800)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5826..6281)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5963..5984)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6175..6194
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc
       61 tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag
      121 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt
      181 taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag atctctgcag
      241 agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc
      301 ctacctgacg accgaccccg acccactgga caagcaccca acccccattc cccaaattgc
      361 gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc
      421 agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct
      481 cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca
      541 ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat
      601 aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc
      661 tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt caagctgcta
      721 gcaaggatcc ccataacttc gtataaagta tcctatacga agttatatca aaataggaag
      781 accaatgctt caccatcgac ccgaattgcc aagcatcacc atcgacccat aacttcgtat
      841 aatgtatgct atacgaagtt atactagagc tttcagaatc gagtagcctc ggggaaatct
      901 gttgcgcaag agatgaagct atcaatagat gacatactcc tcatgtcaat cactccttca
      961 gtcctggcag ggaggggagc cacgggactt ggcatactgg acatctcaag ctcttcggga
     1021 ggcttagact gtggagccat ttcctttgtg ttcaggatag gctgactttg cacctcgttc
     1081 tcgtagcaga acacgttgat gtcgatctgg tccaggggga tgtactcgcc ctcgctggtg
     1141 atcctgctct tcacctcgtt ctcgtagcag aacacgttga tgtcgatctg gtccaggggg
     1201 atgtactcgc cctcgctggt gatcctgctc ttcttgtaca gctcgtccat gcccatcaca
     1261 tcggtaaagg ccttttgcca ctccttgaag ttgagctcgg tcttggagtg cttgagctcc
     1321 gtcttacgga acacgtacat cggctggttc ttcagatagt tagccgccat tggcttggca
     1381 aaggtgtagg tggtccgcgc agtgctcctg tagcgcttgc catttccagt ggtgtaactc
     1441 cacttaaagg tactgatgat ggttttgtcg ttggggtaag tcttcttcga cctgcaccag
     1501 tccgcagcgg tcagcgagtt ggtcatcaca ggaccgtcag cagggaaacc agtccccttc
     1561 acctgggcct ctcctttgat gtggcttccc tcgtaggtgt agcggtagtt aacagtaagg
     1621 gaggcaccat cttcaaactg cattgtgcga tggacttggt atccggagcc atctaccatg
     1681 gcggcctgga aaggcgacat cccgtcaggg tagggcaggt actgatggaa gccatacccg
     1741 atatgaggga ccagaatcca gggggagaac tggaggtcac ccttggtgga cttcaggttt
     1801 aactcctcat aaccatcatt tggattgccg gtgccctgac ccaccatgtc aaagtccaca
     1861 ccgttgatgg agccaaagat gtgtaactca tgtgtcgctg ggagagacct cagcatcttc
     1921 ttgaagatag tctcatgggc aatgaggcac cccattccaa acagtggctt agcgagcaca
     1981 tccaggattc ccatgagaat ggcctcattg tcgacggaca gcacgccact accagacccg
     2041 aaagcccaca cgacaggata aaacacccag aggattgaca ggaagacgag cagactgaca
     2101 taggagctat ggtactcggg gcttttcttc agatcgtctt tccaagtggc gttatacatc
     2161 ccgtatgtca ggactgcctg catcacacat ccagcgataa agtacaccca tttgaacttg
     2221 tgttcatcct cgaagtcgcc cagaatacca aacacgatca tgaaaatgtc tgcgccgatg
     2281 acccaagaaa tcatcactcc gcccatcttg gtcatgacga tgagatcgag caggagcagt
     2341 ggtgtggtca gcacccagtt aatatatctt gcgtagacca cctgcctttc accattcatt
     2401 gtcaggtcct ggtagttcac ggccattgca tagtaagcgg tagcggcgat agtgcagatg
     2461 gcaatgttga tatagtatgc gctcttcagc tctgaaggcc gaaagtgcac agccactcca
     2521 aaagcaatag cagcagcagc cataatagca aagacaatcc actgtgcaat catgccggtc
     2581 tcggtttcca cgtcagccat ggtggcggat cctagtataa cttcgtatag gatactttat
     2641 acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg accctctagt
     2701 ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag ttatgtccct
     2761 cgaagaggtt cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     2821 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     2881 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2941 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3001 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3061 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3121 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3181 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3241 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3301 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3361 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc cgggcggccg cttcgagcag
     3421 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3481 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3541 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     3601 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta
     3661 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     3721 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3781 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     3841 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3901 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3961 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4021 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4081 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4141 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4201 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4261 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4321 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4381 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4441 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4501 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4561 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4621 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4681 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4741 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4801 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4861 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4921 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4981 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5041 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5101 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5161 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5221 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5281 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5341 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5401 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5461 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5521 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5581 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5641 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5701 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5761 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5821 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5881 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     5941 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6001 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6061 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6121 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6181 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6241 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     6301 gcgcaact
//
