LOCUS       sequence-341480-        5098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans mScarlet co-injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Witten G, DeMott E, Huang G, Zelasko F, de Jesus B, Mulchand C, 
            Schuck L, Pullman S, Perez A, Mahableshwarkar P, Wu Z, Cardona EA, 
            Pierce JT, Dickinson DJ, Doonan R
  TITLE     mScarlet and split fluorophore mScarlet resources for plasmid-based 
            CRISPR/Cas9 knock-in in C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 14;2023:10.17912/micropub.biology.000871. 
            doi: 10.17912/micropub.biology.000871. eCollection 2023.
  PUBMED    37396790
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-14"; volume: 
            "2023:10.17912/micropub.biology.000871. doi"; pages: " 
            10.17912/micropub.biology.000871. eCollection 2023"
COMMENT     Sequence Label: pGLOW30
FEATURES             Location/Qualifiers
     source          1..5098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    980..1004
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1860..1884)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2654..2674)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2682..2699)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2682..2698)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2691..2713)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3026..3048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3086..3104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3172..3276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3277..3345,3346..4137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3495..3514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4308..4896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4797..4816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5023..5045
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5042..5058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5042..5058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5078..5098
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 cattttatat ctgagtagta tcctttgctt taaatgtcca taaaactaat tttataatca
       61 ataaaacaac gtttgtaaat caactgagtt tacaagtaga gacattgagg gatactttca
      121 ctatgctaaa gtgataatcg accaaataat aacttcactt tggtatttat tcctgtctta
      181 taaatgttat gtatgaatta aattcatatg catatggctc actctgacaa tttttaataa
      241 tcttccagat caatattgac taccgatgcg ggtggtcttt tgctttgaat tctgctgaac
      301 tttacacccc gaacagcaat gtgtgcttca gcctaaaaaa aagtaagtgt gttaatcagt
      361 gcccccgatt cttcattttt tgcccctctc tcccgtttcg tcggcaaaag aagagaaaat
      421 aaagataagt ctcaagatag gttggtaatc gctaaagtgg ttgtgtggat aagagtagca
      481 aaatggcagg aagagcactt tgcgcgcaca cactgtactc attgttctgg ataaaattct
      541 ctcgttgttt gccgtcggat gtctgcctct ctgcattgag ccggcttctt cactatcttt
      601 agttaaccta aaatgccgtt tcttttctcg tatccccact atcccgttga ggttctctgc
      661 tctcttcgct ccctaccgcc agcgagcaac tatccgtggg ggcgccttgc tcggaagatg
      721 ggggggaaga aagaagattt ttgctatttg cacttgagaa agagactttt cctgcgtcga
      781 tggtcagaga acagtgtgca gacacttttc agctacctag aattacaatt ggatatcccc
      841 gcctcccaat ccacccaccc agggaaaaag aagggctcgc cgaaaatcaa agttatctcc
      901 aggctcgcgc atcccaccga gcggttgact tctctccacc acttttcatt ttaaccctcg
      961 atcgtcagac acagaaatga caagtttgta caaaaaagca ggcttaatgg tctccaaggg
     1021 agaggccgtc atcaaggagt tcatgcgttt caaggtccac atggagggat ccatgaacgg
     1081 acacgagttc gagatcgagg gagagggaga gggacgtcca tacgagggaa cccaaaccgc
     1141 caagctcaag gtcaccaagg taagtttaaa catatatata ctaactaacc ctgattattt
     1201 aaattttcag ggaggaccac tcccattctc ctgggacatc ctctccccac aattcatgta
     1261 cggatcccgt gccttcatca agcacccagc cgacatccca gactactaca agcaatcctt
     1321 cccagaggga ttcaagtggg agcgtgtcat gaacttcgag gacggaggag ccgtcaccgt
     1381 cacccaagac acctccctcg aggacggaac cctcatctac aaggtaagtt taaacagttc
     1441 ggtactaact aaccatacat atttaaattt tcaggtcaag ctccgtggaa ccaacttccc
     1501 accagacgga ccagtcatgc aaaagaagac catgggatgg gaggcctcca ccgagcgtct
     1561 ctacccagag gacggagtcc tcaagggaga catcaagatg gccctccgtc tcaaggacgg
     1621 aggacgttac ctcgccgact tcaaggtaag tttaaacatg attttactaa ctaactaatc
     1681 tgatttaaat tttcagacca cctacaaggc caagaagcca gtccaaatgc caggagccta
     1741 caacgtcgac cgtaagctcg acatcacctc ccacaacgag gactacaccg tcgtcgagca
     1801 atacgagcgt tccgagggac gtcactccac cggaggaatg gacgagctct acaagtagta
     1861 cccagctttc ttgtacaaag tgggtgatat ctgagctccg catcggccgc tgtcatcaga
     1921 tcgccatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     1981 tgctctttct ccctgtgctc caacccccta tttttgttat tatcaaaaaa cttctcttaa
     2041 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     2101 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     2161 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
     2221 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     2281 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
     2341 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
     2401 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt
     2461 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
     2521 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
     2581 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa
     2641 acataactgt ttccaacttt attatacata gttgataatt cactggccgt cgttttacaa
     2701 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2761 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2821 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2881 tcacaccgca tatggtgcac tctcagtgca atctgctctg atgccgcata gttaagccag
     2941 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3001 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3061 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3121 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3481 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3781 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4021 tcagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4441 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4921 cggttcctgg tcttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4981 tctgtggata accgtattac cgcctttgag tgagctgata ccagcggata acaatttcac
     5041 acaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttg
//
