LOCUS       sequence-341481-        6622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans mScarlet co-injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Witten G, DeMott E, Huang G, Zelasko F, de Jesus B, Mulchand C, 
            Schuck L, Pullman S, Perez A, Mahableshwarkar P, Wu Z, Cardona EA, 
            Pierce JT, Dickinson DJ, Doonan R
  TITLE     mScarlet and split fluorophore mScarlet resources for plasmid-based 
            CRISPR/Cas9 knock-in in C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 14;2023:10.17912/micropub.biology.000871. 
            doi: 10.17912/micropub.biology.000871. eCollection 2023.
  PUBMED    37396790
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-14"; volume: 
            "2023:10.17912/micropub.biology.000871. doi"; pages: " 
            10.17912/micropub.biology.000871. eCollection 2023"
COMMENT     Sequence Label: pGLOW31
FEATURES             Location/Qualifiers
     source          1..6622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2504..2528
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3384..3408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4178..4198)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4206..4223)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4206..4222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4215..4237)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4550..4572
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4610..4628)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4696..4800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4801..4869,4870..5661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5019..5038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5832..6420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6321..6340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6547..6569
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6566..6582
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6566..6582
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6602..6622
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 agtgattata gtctctgttt tcgttaattt tgaattttgc ttgataaggc tgcaacaaag
       61 atcaggttga catatatttt cagtaattta ttttaacctg tactctatca ctgccggcta
      121 taataagttc ttgaataaaa taattttccc gacaaaacat gagtatttct ttcgaaaata
      181 aaagtgcagg ctaattagag attattctgt aattaactgc ataatttgtc acgtgccata
      241 gttttacatt ccactacgtc atagttctta aaatactaat ctcctgaaaa tagaagtagg
      301 tgaagaaagt ttaattatca gttctaaaat gacaattgat ctttggaata tgttctgaaa
      361 ctaccgatca ttgaacagat gctatttgaa tgatatagaa ttgtatattt gcaatttctg
      421 aaacgcgttc ttaaaggcac acagattaat tcaaaagggt ctggccgcaa aaaggtttat
      481 ggtggccgat tttgagtttt gtgtgtgatt gctttttcac aatcagtgtt ttcaggatta
      541 tgtgatgaac tagatcttca agtttcgtta catttcatat gttttcggaa ctcacgaagt
      601 acatattggg tattgtgctc aaaaaattca gcaatcagct tcgctccgct gactttagaa
      661 cccaaaaaaa tagtatggcc aaactgactg tgttacgatc atttcaattt ttcaatacat
      721 atttaagatt tctaagagta agaaggtcaa aaactgttct ggaatacata tatatttttc
      781 aggttacaat tagtcaaaaa gtgcactgaa atatacgttt taatttcacg aataacccaa
      841 ttagttcaat gtatttttgg tcaaccaacg ttaaagtttg gcttccaacc aattatcatt
      901 tctgatcaac cacaatgttt tttctttatc tgcaagttaa ttttttattt ttatccagat
      961 gtttggcata tttttcaatt cttcactagc gcccacttct tgcacttccg gcgccctgaa
     1021 tctaatgcat ctgttgcaag aattgaaaga ccaatcaaca cattgttttc ttcacgagat
     1081 actgaagaaa atgaataaaa acagagaaaa agagccatgt gattagtgac aactgttgct
     1141 aacagatacc atagcttgga cttggtacgt gatggcaacg tatgggtcaa caaaaatgat
     1201 tgcagagggg gtgcaaaaca gtcaagtcga gaaaatatga aaaacagaaa acaaagaaca
     1261 gaaaaatggg tttgagagtc agtataattt ataaaagaaa aattgtacat agaaattaac
     1321 catttttgta gaagaagtta tttttcaagc atcgttaaaa attattcaaa gcaccttatt
     1381 tcatatttaa ttttaaacat ggttaaatga acaacacggt gcgcaatcag gaaaacttga
     1441 aatctgaaac tgttgttgtg atcttcttcg caactgttca gatagcacta gtgtaatgtt
     1501 aagagtgcgc gaatataatg gaatataatg gatcacacct cctgccatca ggtaaacgtc
     1561 tctgttatca catatttcca actattaaat ttttaccttt tacagtttta catttttttg
     1621 aaaaaagtaa ctttttgtct tcaaaatccc tgacgaaaat atcaaatatt ttaatcgaga
     1681 ctgcagagga accgattgat gatttggaaa atccagcttt acctgtgtaa gaactgaaaa
     1741 gtttcataac cctagggtat tcccagttac attccccact ggctaacaat agcacccagt
     1801 ttttcatcac ctttcttcaa atttctcggc gatttgttaa aaacaaaatt tgtgtccctt
     1861 ctctgatatc tctatgtctc taaacacaag ttcatcggaa aacgaaggag ggtaggtgtt
     1921 ggttgggctc ccgaagtgaa aatagaagag caagaataga atattagaga gagagtgcag
     1981 agagggcggg atagctcccg ggattccgtt ttcttcttct ttatcttcaa cgatgatgtg
     2041 tgtgcgtgtt gtatagattc tgttgctccc ccacaactcg ctccgaaggc tcaatacaat
     2101 tcaattgata ttggaggaga gcctaccgga gtgggaggat aagaagaaac ataagaagaa
     2161 gaagaagaag aagcatgctt ctggtttttg atgctatgaa aacggcacaa aaagatgatt
     2221 gaggtccctt ttcaatacct tctctcatct ttcaaatccc attgaaacct aaaacttctc
     2281 accacgcttt accattgttc tccaaaaact tatagcaatg tctataactt ttttatctct
     2341 gaaaagcagt gttccatttt tctttttcct attttatttc aattgtttct cacatttcgt
     2401 ttggattctt tgcttgtcaa ccagcttctt cttccacttt taccgtctaa ttttcagggc
     2461 agggagccat caaacccacg accactagat ccatctagaa atgacaagtt tgtacaaaaa
     2521 agcaggctta atggtctcca agggagaggc cgtcatcaag gagttcatgc gtttcaaggt
     2581 ccacatggag ggatccatga acggacacga gttcgagatc gagggagagg gagagggacg
     2641 tccatacgag ggaacccaaa ccgccaagct caaggtcacc aaggtaagtt taaacatata
     2701 tatactaact aaccctgatt atttaaattt tcagggagga ccactcccat tctcctggga
     2761 catcctctcc ccacaattca tgtacggatc ccgtgccttc atcaagcacc cagccgacat
     2821 cccagactac tacaagcaat ccttcccaga gggattcaag tgggagcgtg tcatgaactt
     2881 cgaggacgga ggagccgtca ccgtcaccca agacacctcc ctcgaggacg gaaccctcat
     2941 ctacaaggta agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggt
     3001 caagctccgt ggaaccaact tcccaccaga cggaccagtc atgcaaaaga agaccatggg
     3061 atgggaggcc tccaccgagc gtctctaccc agaggacgga gtcctcaagg gagacatcaa
     3121 gatggccctc cgtctcaagg acggaggacg ttacctcgcc gacttcaagg taagtttaaa
     3181 catgatttta ctaactaact aatctgattt aaattttcag accacctaca aggccaagaa
     3241 gccagtccaa atgccaggag cctacaacgt cgaccgtaag ctcgacatca cctcccacaa
     3301 cgaggactac accgtcgtcg agcaatacga gcgttccgag ggacgtcact ccaccggagg
     3361 aatggacgag ctctacaagt agtacccagc tttcttgtac aaagtgggtg atatctgagc
     3421 tccgcatcgg ccgctgtcat cagatcgcca tctcgcgccc gtgcctctga cttctaagtc
     3481 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     3541 ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta
     3601 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     3661 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     3721 cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac
     3781 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca
     3841 cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt
     3901 ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg
     3961 tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta
     4021 ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg
     4081 tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga
     4141 cagaatacat tcccaatata ccaaacataa ctgtttccaa ctttattata catagttgat
     4201 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4261 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4321 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     4381 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc
     4441 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4501 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4561 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4621 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5041 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5341 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5521 cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt agttatctac
     5581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5941 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6421 aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt tgctcacatg
     6481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6541 gataccagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     6601 tcaactttgt atagaaaagt tg
//
