LOCUS       sequence-341515-        6016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High-copy number plasmid (pMT335) harboring the mScarletI gene for 
            its cytoplasmic expression, inducible by vanillate (sequence of 
            mScarletI optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Mahecic D, Stepp WL, Zhang C, Griffie J, Weigert M, Manley S
  TITLE     Event-driven acquisition for content-enriched microscopy.
  JOURNAL   Nat Methods. 2022 Sep 8. pii: 10.1038/s41592-022-01589-x. doi: 
            10.1038/s41592-022-01589-x.
  PUBMED    36076039
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Sep 8. pii: 10.1038/s41592-022-01589-x. doi: 
            10.1038/s41592-022-01589-x."
COMMENT     Sequence Label: pMT335-mScarletI
FEATURES             Location/Qualifiers
     source          1..6016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(234..767)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(956..961,962..978,979..984))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(2732..2748)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2732..2748)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2780..2799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2781..2799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2809..2826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2809..2825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2818..2840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(3534..4196)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      4197..4968
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ctggacgccc tggggccgtt gaatcgggat atgcaggcca aggccgccgc gatcatcaag
       61 gccgtgggcg aaaagctgct gacggaacag cgggaagtcc agcgccagaa acaggcccag
      121 cgccagcagg aacgcgggcg cgcacatttc cccgaaaagt gccacctgcg gcgttgtgac
      181 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
      241 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
      301 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
      361 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
      421 tctccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
      481 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
      541 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
      601 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
      661 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
      721 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
      781 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
      841 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      901 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
      961 acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg
     1021 tccatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1081 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagttt
     1141 ttatataagc tttcagtcgg cgcgaatgct ccacgccgcg cccagcggcg cgccggcgct
     1201 cgccgcggcc tcgaagacct tggcgttacg aatggcggcc agggcatggt cgcgcatgat
     1261 ccgctcggcc ccctcggcgt cgccgcacga gaccgcgtcc agcaccgcct gatgctggcg
     1321 atgggcggcc agaaggtgtt cgtactcggc cggcaagtcc atcagatcca gggccagcgc
     1381 accggccgcc gcgaacggct cgaaaccatt gcgcgccagg gcgctctcga cagcgccgtt
     1441 gcccgcagcg ctgaccagcg tgtcatggaa ggcctgattg tacgcggcgt agcgatccag
     1501 gtcctcgccg ttaagccgcc cagccgcgaa cagcgcttcg ccctcggcga tcaaggcgac
     1561 gaaccgcgcg tgggtctccg ccgtcattcc gcgctcggcc agacgccggg ccgcaaagcc
     1621 ctccagcacc cctcgcacct cgatggcgtc gcggatctgg tcgctggaca cgcctcgggc
     1681 ggcataaccc cgcgcgccca gccgcaccac gagcccttcc tgctccagac tccgcaaggc
     1741 cgtgcgcacg ggcatgcgcg agacgcccag cgccgccgct gtcgggattt cggcgatacg
     1801 ctcgccgctc ttgatctcgc ccgaggcgat catcttgcga agagccatca tcacgcgctg
     1861 gcccggcttt atgcgcggca tgtccatggc tggactctag ccgaccgact gagacgctca
     1921 caagcgttca attggatcca atcttgacgt ccgtttgatt acgatcaaga ttggatccaa
     1981 taggtcgccg aaccacgatg cgaggaaacg catatggtca gcaagggcga ggcggtgatc
     2041 aaggagttca tgcgcttcaa ggtccacatg gagggctcga tgaacggcca cgagtttgag
     2101 atcgagggcg aaggtgaagg gcggccctat gaaggcacgc aaacggccaa gttgaaggtc
     2161 accaagggtg gcccgctgcc gttcagctgg gacatcctgt cgccgcagtt catgtacggc
     2221 agccgcgcct tcatcaagca ccccgccgat atcccagact attacaaaca gtcgttcccc
     2281 gagggcttca agtgggagcg ggtcatgaac ttcgaggatg gaggcgcggt tacggtgacc
     2341 caggacacct cgctggagga cgggacgctg atctacaagg tgaagctgcg cggcaccaac
     2401 ttcccgcccg atgggcctgt gatgcagaag aagaccatgg gctgggaagc gtccaccgag
     2461 cgtctctatc cggaagacgg cgtgctgaag ggcgacatta agatggccct gcgacttaag
     2521 gacggcggcc gctatctggc cgacttcaag acgacctaca aggccaagaa gccggtgcag
     2581 atgccgggcg cttataatgt cgaccgcaag ctcgacatca ccagccacaa cgaggactac
     2641 accgtcgtcg agcagtacga gcgctccgaa ggtcgccatt cgaccggcgg catggacgag
     2701 ctgtacaaat aggaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc
     2761 gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     2821 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2881 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2941 gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg
     3001 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgtactgcg atgagtggca
     3061 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc
     3121 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc
     3181 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta
     3241 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag
     3301 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg
     3361 ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
     3421 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc
     3481 tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg
     3541 cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
     3601 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
     3661 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
     3721 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
     3781 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
     3841 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
     3901 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
     3961 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
     4021 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc
     4081 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc
     4141 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt
     4201 atctccatgc ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt
     4261 tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt
     4321 taacctacgc aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt
     4381 tttaagttgt tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc
     4441 aaagaagggc acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa
     4501 aagtggcccc tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc
     4561 gctgccccct tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc
     4621 ttcgcccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca
     4681 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca
     4741 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat
     4801 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag
     4861 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc
     4921 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg
     4981 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag
     5041 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag
     5101 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga
     5161 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa
     5221 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga
     5281 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac
     5341 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt
     5401 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga
     5461 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga
     5521 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt
     5581 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca
     5641 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg
     5701 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg
     5761 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc
     5821 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc
     5881 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg
     5941 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga
     6001 gcgcctgaag cccgtt
//
