LOCUS       sequence-341516-        7268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose 
            (sequence of sfGFP optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Mahecic D, Stepp WL, Zhang C, Griffie J, Weigert M, Manley S
  TITLE     Event-driven acquisition for content-enriched microscopy.
  JOURNAL   Nat Methods. 2022 Sep 8. pii: 10.1038/s41592-022-01589-x. doi: 
            10.1038/s41592-022-01589-x.
  PUBMED    36076039
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Sep 8. pii: 10.1038/s41592-022-01589-x. doi: 
            10.1038/s41592-022-01589-x."
COMMENT     Sequence Label: Pxyl-ftsZ-sfGFP
FEATURES             Location/Qualifiers
     source          1..7268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3694..3705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(4357..4359,4360..5073)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5092..5108)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5119..5136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5119..5135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5128..5150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5333..5921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5413..5432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6331..7146)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6331..7146)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7054..7073
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7138..7157
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 cgctaccgga ctcagatcca gatcgaaatg taccggctga actggtggaa gcacgttgag
      541 cctcgtcaag gactcatgaa gcgcgtccct gctctcagtc gcctgctcgt tacgcctgag
      601 gtgtcaaaac accgtctgtt catttggcta gacgctagag ttcttccgga tcacaagctc
      661 caggtcgtta cactcgatga cgactgctca ttcggagttt tacactcaag gttccacgag
      721 gtgtgggctc ttgcagcggg gtcgtggcac ggctcaggca atgatccgcg ctacaccatc
      781 tccaccacct tcgagacctt ccccttcccg gaaggcctga cgcccaacat agccgccgtc
      841 gactatgagg gcgacccgcg cgcccaggcc atcgccgcgg cggcggccga gctgaaccgg
      901 ctgcgggaag cctggctcaa tccgcccgac ctggtgcgga tcgagcccga ggtcgtgccc
      961 ggctatcccg accgcgtgct gcccgtcagc cccgaggcgg gcgccgagct gaagaagcgc
     1021 accctgacca acctctacaa ccagcgcccg gcctggctgg acatggccca ccagcgcctg
     1081 gacgccgccg tcgccgccgc ctacggctgg cccgacggcc tgaccgacga cgagatcctg
     1141 gagcgcctgt tcgccctcaa ccaggagcgc gcggcggcgg ggcggtaggt tagacccctc
     1201 caaaaccccg acctccccgg cgaaggccgg ggtccagtta catccggaac gcttggagtg
     1261 ggcccgccta cggcctcgac caacccgcag tcggcaaggg gttcgatctg gaccccggca
     1321 ttcgccgggg aggtcggcag agaggtcaga accgcgtctt gcagccctgc ttcagatgaa
     1381 ccccgcgcag gaagccgcgg cgggccaggc cggcgtcgac ggcggcgttc atctggcggt
     1441 cggcgggggc agcgccggtg acttcgcgca ccaggccccg gaacgggatg atgctgttga
     1501 tcagggtctt gccaccggcc tcggccagct tgccggctcg gttttcggcg tactcggcgc
     1561 cgaccttgta gtcgggcccc agcgccgcgt tcagcgcctt cacctcattg gccagggcct
     1621 ggcaggactt cagacccgcg aggctgtagg ggtcgtcaaa ggccttggcc aggaccggcg
     1681 gcacctcgcg cttggacagg ccgacgtcgc gcaccggctg ggtgccgatg tcgacggcct
     1741 tcttgcccgc ctcgccccag cggccggacg gttcctcggg cttggccggc ggcttgacct
     1801 gggcgtgggc gctggaggcc agcagcacgg cggcggctcc ggccatgatc agcagcttgt
     1861 tcggcatcaa aagcgccctc ccgacaaaac acgccacggg aacggcaagc ctcgccgccg
     1921 gttcccgccc gcaagtccct cggcacgaac tctaaggcct gcgcacgatc catcgccacc
     1981 acaaagcgtg tcatcccggc cgaagcgcag cggagagccg ggacccaggg ccacaagcac
     2041 ggcgcttcgt caccccctgg gtcccggata gcgctccgcg cttccgggat gacacgggtt
     2101 taagcacttg ggtgagaagc cctagttcgg cgcgccgccc gcttccaggt cgaacgcggc
     2161 gtcgaaggcg tcgtcgccag gaaccgtccc ttccagggcg gcgtcgaaca ggtcttcgag
     2221 cgatgaccag atgaaggcca tctgctccat gatcgtcggg acgctggccg gatcgaagcc
     2281 ggggtagagg tcttccacca gccacaggcc cgtgccggga tcgaaggcga agcggcagcc
     2341 gatcaggcgg aactgggcgc gggccatggt ctcgaacagg gccgtcaggt cgcgggcggc
     2401 gtccaggtcg tcgtggtcca gcaccaggca gcggatctgc agccagccgt ggtcgggcag
     2461 caggtagaag gcgccctcgt cctgatcctc gcccgaaacc tccagccccc ggtcgatggc
     2521 ttcgacgaca tagccggccg cgcggcaggt gtcggtgagc gcggccagca gggcggcttc
     2581 ctggtcaggg gtcaggtcgg tcatgggcaa gaggtccagg tcgtggtttg tcggcggctt
     2641 ctagcatgga ccgcccgcgc ccgtgaggcc gaggatttcg cgctggtcag acaacctact
     2701 tgccgtcccc acatgttagc gctaccaagt gccgacgaac gcgcgccgcc gacggtgtcg
     2761 gcgcttcaga cgctcgagtt ttggggagac gaccatatgg ctatttctct ttccgcgccg
     2821 cgtacgaccg agctgaagcc gcgtatcgtg gttttcggcg tcggcggcgc tggcggcaac
     2881 gccgtgaaca acatgatcga ggcgggcctc gaaggtgtgg agttcgtggt ggccaacact
     2941 gacgctcaac agcttcagtt cgccaagacg gaccgtcgca tccagctggg cgtgcaaatc
     3001 acccagggcc tgggcgccgg cgcgcacccc gaggtgggca tgagcgcggc cgaagagagc
     3061 ttccccgaga tcggcgagca cctcgacggc gcccacatgg tcttcatcac cgccggtatg
     3121 ggcggcggca ccggcaccgg cgcggccccg atcatcgcca agtgcgcccg tgagcgcggc
     3181 atcctgaccg tcggcgtcgt gaccaagccc ttccacttcg aaggccgtca ccgcatgcgc
     3241 ctggccgaca gcggcatcca ggagctgcag cgctatgtcg acaccctgat cgtcattccg
     3301 aaccagaacc tgttccgcgt cgccaacgag cgcacgacct tcgccgaagc cttcggcatg
     3361 gccgaccagg tgctgcactc gggcgtccgc tcgatcaccg acctgatggt cctgccgggc
     3421 ctgatcaacc tcgacttcgc cgacgtccgc acggtcatga ccgagatggg caaggcgatg
     3481 atgggcaccg gcgagggcac cggcgaagac cgcgccctga tggccgctca gaacgccatc
     3541 gctaacccgc tgctggacga agtctcgctg aagggcgcca aggccgttct ggtcaacgtg
     3601 accggcggca tggacatgac cttgctggaa gtcgacgagg ccgccaatgc gatctccgac
     3661 caggtcgatc cggaagccaa catcatcttc ggcgcggcct tcgatccgtc gctggaaggc
     3721 gtcatccgcg tgtcggtcgt cgccaccggc atggacggcg cctcgatcgc ccagatcgag
     3781 cccaagcccg tgtcgcgcaa catctcggcc gccccgctga tcgccgagac ctcgcgtcct
     3841 gcgccgcagc ccgagccggc ccgtccgact gcgcgctacg aagccgctcg tccggccgaa
     3901 cgtcccgtgg ccttcgcgcc cgagccggcg ccggaacccg agatcgtgat gtcggccccg
     3961 cagcctgagc cggaagccga gctttactac gacgagccga ctgtagctga agaaccgcgt
     4021 gtttccgcgg ctccggcccg gtcggtgaac cgcatcgtcg atccgctggt cgacgacgtc
     4081 gctgaggagc cgctgttccc cgagaacaac tactacgaag agcgtcgtcc ccagaagcag
     4141 ggcgggttct tctcgatgtt cggtggcggt cgccagcgct acgagcaaca ggcgtctgcg
     4201 ccgcaggccc aagcccgctc ggcccagagc gcgcgtccgc aactgcagcc gatcgaaacg
     4261 ccgcaggccg atgacgccga ggatctggag atcccgtcgt tcctgcgccg cctggccaac
     4321 gagaattcga acgttacgcg tcaccggtcg gccaccatga gcaagggcga ggagctgttc
     4381 accggcgtgg tgccgattct cgttgaactg gacggtgatg tcaatggcca taaattttcg
     4441 gtgcggggcg aaggcgaggg cgacgccacc aacggcaagc tcacgctcaa gttcatctgc
     4501 accacgggca agctgcccgt gccctggccg accctggtga cgacgctgac ctatggggtc
     4561 cagtgcttct cccgctatcc ggatcacatg aagcgtcacg acttcttcaa gagcgccatg
     4621 cctgaaggct atgtccaaga gcgcacgatc agcttcaaag acgacggcac ctacaagacc
     4681 cgggccgagg tcaagttcga gggcgacacc ctggtcaacc gcatcgagct gaaggggatc
     4741 gacttcaagg aagacggtaa catcctgggc cacaagctgg aatacaactt caactcgcac
     4801 aacgtttaca tcacggccga caagcagaag aacggcatca aggccaattt caagatccgc
     4861 cacaatgtcg aggacggatc tgtgcagctg gcggatcatt accagcagaa caccccgatc
     4921 ggcgatgggc cggtgctgct gccggacaac cactatctgt cgacccagtc ggtcctgtcc
     4981 aaggacccca acgagaagcg cgaccacatg gtccttttgg agttcgtcac cgcggcgggc
     5041 atcacccacg gcatggacga gctctacaag taagctagct gcagcccggg ggatccacta
     5101 gttctagagc ggccattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5161 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagcggta tcagctcact
     5221 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6001 tatcaaaaag gatcttcacc tagatccttt tagggggggg ggggcgctga ggtctgcctc
     6061 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
     6121 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
     6181 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
     6241 cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg
     6301 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     6361 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     6421 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     6481 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     6541 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     6601 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     6661 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     6721 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     6781 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     6841 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     6901 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     6961 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     7021 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     7081 atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg
     7141 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     7201 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc
     7261 cccccccc
//
