LOCUS       sequence-341518-       12091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression of 60meric GEM monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UBC1cer40GEM
FEATURES             Location/Qualifiers
     source          1..12091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2471..3181
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2498..2526)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3121..3141
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3829..3845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3829..3845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3842..3864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3853..3869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3877..3883,3884..3901,3902..3907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3922..3943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4134..4810
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     4747..4767
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4877..5902
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5943..6117
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6195..6219
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6644..7438
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7525..8113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8014..8033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8267..8284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8299..8439
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8365..8384)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8525..8547
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8783..8977
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9043..10116)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10545..11174)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccgttt tgctgatagg tttcgtcagg attgatatat gttcgtaggt agcagaagag
      721 actaaaagta gtttattttt tcgtcctttt gaccaaatgt tcaaaagcca catatcattt
      781 cgtggtaagc ggttgtatct caaaacatca gtctgatctt agttgttttt gatttaagag
      841 caaatctcaa tttgaagtct gagtacgagc tagtatgatc caaatgccta ggctttgaga
      901 cgaggcttga agatgaattg gtttgtctag agagattgga gaaacaagac ataccacaaa
      961 aacatttctc tctagtaaca aaaacatcac tctactctac tcttgtggtt tgggtctgga
     1021 ttttagtccg gttagtggta caatggttta tattggttta ttagaaatat atagtttacg
     1081 ctacattaag aaaagtactc taatttgtca tctgaagatt tacataaaaa agattacatg
     1141 tcatcgttgt atgttaacta acactaatct cccggttaaa gtgtagattg ccctaaacgg
     1201 tttaattccg gttaggaaca aaccaaccac tcgctcggtc gtccaaaggg caaagcgaat
     1261 ccaactttac agatatgtca gagaggagcc aagcttctag aagatcattc attcatcact
     1321 tatacgatct cttcccaaat tattgatttt ttttacccat ttctctcatc gcacggtgat
     1381 attgagaatc gccgacctga atcgatcgga aaactttctc tgattaccgg cggtcaacac
     1441 cgctgaacac atatgtttgt ttgacgacct cttctctccg cgatctttac ctcaacaacg
     1501 agatctgttt ccacggtacc attttcatcc gatctctgtt cttctatgtt tgtttactgg
     1561 atccgttgat tttactttca tcttatcatt tgtgtgtggc tattttggtg aattgatgcg
     1621 atcttaagct gtttctaggt tcaagcctta tatgtttctt ctaagctaat cataggtcgt
     1681 agacttgtta ggaagattcg aatctagtac aaagttagca actttagcat acgtaaatcg
     1741 ttagtagtaa tttctagatt gatagaggtt cgagacctta aaaggtgatg aatatttggt
     1801 gggttttgtt tattagaaag aaaggaggat gatggatccg gtagtagtgc tgggtttgtg
     1861 tttgagctgt ctcctgttgc tgagtctctg gaagcaatcc tacggcggtg gcaaactggg
     1921 cggaggtggc ggtggagcta gcatgcagat ttacgaaggc aaactcaccg ctgaaggatt
     1981 gcgttttggt attgtggcct cccggtttaa tcatgccctg gtggacaggc tggtcgaggg
     2041 agccatcgac tgtatcgtgc ggcatggcgg tagggaggaa gatataaccc tggttcgagt
     2101 cccaggaagt tgggaaatac cagtggctgc cggtgagttg gctcgtaagg aggatattga
     2161 cgctgttatt gcgataggag ttctcatccg aggagcgact cctcattttg actatatagc
     2221 ctcggaagtc tccaagggac tcgccaactt gagcctcgag ttgaggaaac cgatcacctt
     2281 cggagttata actgccgata cgttggaaca agcgatcgag cgtgctggta cgaagcatgg
     2341 caataagggt tgggaggcag ctctgagtgc catcgagatg gcgaatctct tcaaaagcct
     2401 cagatctggc agcggtagtg ggtcgggatc aggctccggt cgacggatcc ccgggttaat
     2461 taagctgact agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     2521 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     2581 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccgtggcc
     2641 aacacttgtc actactttct cttatggtgt tcaatgcttt tcaagatacc cagatcatat
     2701 gaagcggcac gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat
     2761 cttcttcaag gacgacggga actacaagac acgtgctgaa gtcaagtttg agggagacac
     2821 cctcgtcaac aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg
     2881 ccacaagttg gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa
     2941 gaacggcatc aaagccaact tcaagacccg ccacaacatc gaagacggcg gcgtgcaact
     3001 cgctgatcat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     3061 ccattacctg tccacacaat ctaagctttc gaaagatccc aacgaaaaga gagaccacat
     3121 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     3181 agctagccca tggtaagctt tttgtgatct gatgataagt ggttggttcg tgtctcatgc
     3241 acttgggagg tgatctattt cacctggtgt agtttgtgtt tccgtcagtt ggaaaaactt
     3301 atccctatcg atttcgtttt cattttctgc ttttctttta tgtaccttcg tttgggcttg
     3361 taacgggcct ttgtatttca actctcaata ataatccaag tgcatgttaa acaatttgtc
     3421 atctgtttcg gctttgatat actactggtg aagatgggcc gtactactgc atcacaacga
     3481 aaaataataa taagatgaaa aacttgaagt ggaaaaaaaa aaaacttgaa tgttcactac
     3541 tactcattga ccataatgtt taacatacat agctcaatag tatttttgtg aatatggcaa
     3601 cacaaacagt ccaaaacaat tgtctcttac tataccaaac caagggcgcc gcttgtttgc
     3661 cactctttgt gtgcaatagt gtgattacca catctccaca ttcaatatat tccctgaatt
     3721 atctgacgat tttgatggct cactgttttc ccaagtctgg taccccacct acatggggat
     3781 cctaactcga gctgcaggct tcggtaccga gctcgaattc gtaatcatgt catagctgtt
     3841 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3901 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3961 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4021 ggggagaggc ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact
     4081 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
     4141 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     4201 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
     4261 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     4321 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     4381 tgtgaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
     4441 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
     4501 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
     4561 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
     4621 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
     4681 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
     4741 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     4801 gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc
     4861 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     4921 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     4981 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     5041 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     5101 ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct cccgccgttc
     5161 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tacaaccggt
     5221 cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     5281 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     5341 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     5401 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     5461 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     5521 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     5581 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     5641 gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg accaactcta
     5701 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     5761 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     5821 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     5881 tcgtccgagg gcaaagaaat agagtagatg ccgaccggga tctgtcgatc gacaagctcg
     5941 agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg
     6001 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     6061 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc
     6121 cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     6181 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     6241 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     6301 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     6361 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     6421 gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg
     6481 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     6541 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
     6601 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat
     6661 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     6721 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     6781 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     6841 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     6901 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     6961 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     7021 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
     7081 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa
     7141 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     7201 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     7261 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     7321 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     7381 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta
     7441 cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8161 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8221 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8281 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     8341 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8401 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     8461 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8521 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     8581 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     8641 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     8701 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     8761 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     8821 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     8881 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     8941 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     9001 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     9061 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     9121 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     9181 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     9241 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     9301 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     9361 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     9421 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     9481 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     9541 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     9601 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     9661 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     9721 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     9781 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     9841 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     9901 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     9961 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
    10021 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
    10081 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
    10141 ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt
    10201 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    10261 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    10321 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    10381 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    10441 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    10501 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    10561 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    10621 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    10681 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    10741 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    10801 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    10861 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    10921 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    10981 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    11041 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    11101 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    11161 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    11221 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    11281 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    11341 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    11401 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    11461 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    11521 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    11581 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    11641 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    11701 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    11761 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    11821 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    11881 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    11941 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    12001 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    12061 gccggccgcg tgcagttcgg cccgttggtt g
//
