LOCUS       sequence-341537-       13352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of cytoplasmic pspCas13b protein tagged with 
            3xHA-T2A-BFP for gene silencing in mamallian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Fareh M, Zhao W, Hu W, Casan JML, Kumar A, Symons J, Zerbato JM, 
            Fong D, Voskoboinik I, Ekert PG, Rudraraju R, Purcell DFJ, Lewin SR,
            Trapani JA
  TITLE     Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and 
            circumvents its mutational escape through mismatch tolerance.
  JOURNAL   Nat Commun. 2021 Jul 13;12(1):4270. doi: 10.1038/s41467-021-24577-9.
  PUBMED    34257311
REFERENCE   2  (bases 1 to 13352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-13"; volume: "12(1):4270. doi"; pages: " 
            10.1038/s41467-021-24577-9"
COMMENT     Sequence Label: Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..13352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(120..140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        125..454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      305..440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     367..386
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        502..549
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             568..942
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1109..1128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1163..1182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1242..1258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1242..1258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1255..1277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1266..1282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1290..1296,1297..1314,1315..1320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1335..1356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3443..3462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3634..4013
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4015..4213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4168..4188
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4231..4411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4458..4583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5076..5309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5494..5538
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5836..5953
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6078..7256
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6309..7247
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7204..7224
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             10551..10577
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             10578..10643
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10662..10715
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10716..11417
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    11440..12028
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11493..11513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11911..11922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12031..12047)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12032..12048)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             12553..12733
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12759..12776)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12765..12989
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(13035..13352,1..111)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13122..13141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(13332..13352,1)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
       61 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
      121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      361 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      421 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
      481 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
      541 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
      601 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
      661 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
      721 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
      781 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
      841 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
      901 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
      961 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     1021 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     1081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     1201 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     3421 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     3481 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     3541 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     3601 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     3661 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3721 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3781 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3841 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3901 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3961 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     4021 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     4081 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     4141 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     4201 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     4261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4381 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     4441 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     4501 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     4561 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4621 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     4681 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     4741 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     4801 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     4861 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     4921 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     4981 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     5041 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     5101 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     5161 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     5221 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     5281 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     5341 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     5401 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     5461 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     5521 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     5581 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     5641 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     5701 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     5761 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     5821 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     5881 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     5941 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaatgc
     6001 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     6061 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     6121 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     6181 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     6241 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     6301 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     6361 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     6421 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     6481 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     6541 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     6601 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     6661 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     6721 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     6781 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     6841 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     6901 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     6961 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     7021 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     7081 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     7141 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     7201 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta
     7261 cggccaccat gaacatcccc gctctggtgg aaaaccagaa gaagtacttt ggcacctaca
     7321 gcgtgatggc catgctgaac gctcagaccg tgctggacca catccagaag gtggccgata
     7381 ttgagggcga gcagaacgag aacaacgaga atctgtggtt tcaccccgtg atgagccacc
     7441 tgtacaacgc caagaacggc tacgacaagc agcccgagaa aaccatgttc atcatcgagc
     7501 ggctgcagag ctacttccca ttcctgaaga tcatggccga gaaccagaga gagtacagca
     7561 acggcaagta caagcagaac cgcgtggaag tgaacagcaa cgacatcttc gaggtgctga
     7621 agcgcgcctt cggcgtgctg aagatgtaca gggacctgac caaccactac aagacctacg
     7681 aggaaaagct gaacgacggc tgcgagttcc tgaccagcac agagcaacct ctgagcggca
     7741 tgatcaacaa ctactacaca gtggccctgc ggaacatgaa cgagagatac ggctacaaga
     7801 cagaggacct ggccttcatc caggacaagc ggttcaagtt cgtgaaggac gcctacggca
     7861 agaaaaagtc ccaagtgaat accggattct tcctgagcct gcaggactac aacggcgaca
     7921 cacagaagaa gctgcacctg agcggagtgg gaatcgccct gctgatctgc ctgttcctgg
     7981 acaagcagta catcaacatc tttctgagca ggctgcccat cttctccagc tacaatgccc
     8041 agagcgagga acggcggatc atcatcagat ccttcggcat caacagcatc aagctgccca
     8101 aggaccggat ccacagcgag aagtccaaca agagcgtggc catggatatg ctcaacgaag
     8161 tgaagcggtg ccccgacgag ctgttcacaa cactgtctgc cgagaagcag tcccggttca
     8221 gaatcatcag cgacgaccac aatgaagtgc tgatgaagcg gagcagcgac agattcgtgc
     8281 ctctgctgct gcagtatatc gattacggca agctgttcga ccacatcagg ttccacgtga
     8341 acatgggcaa gctgagatac ctgctgaagg ccgacaagac ctgcatcgac ggccagacca
     8401 gagtcagagt gatcgagcag cccctgaacg gcttcggcag actggaagag gccgagacaa
     8461 tgcggaagca agagaacggc accttcggca acagcggcat ccggatcaga gacttcgaga
     8521 acatgaagcg ggacgacgcc aatcctgcca actatcccta catcgtggac acctacacac
     8581 actacatcct ggaaaacaac aaggtcgaga tgtttatcaa cgacaaagag gacagcgccc
     8641 cactgctgcc cgtgatcgag gatgatagat acgtggtcaa gacaatcccc agctgccgga
     8701 tgagcaccct ggaaattcca gccatggcct tccacatgtt tctgttcggc agcaagaaaa
     8761 ccgagaagct gatcgtggac gtgcacaacc ggtacaagag actgttccag gccatgcaga
     8821 aagaagaagt gaccgccgag aatatcgcca gcttcggaat cgccgagagc gacctgcctc
     8881 agaagatcct ggatctgatc agcggcaatg cccacggcaa ggatgtggac gccttcatca
     8941 gactgaccgt ggacgacatg ctgaccgaca ccgagcggag aatcaagaga ttcaaggacg
     9001 accggaagtc cattcggagc gccgacaaca agatgggaaa gagaggcttc aagcagatct
     9061 ccacaggcaa gctggccgac ttcctggcca aggacatcgt gctgtttcag cccagcgtga
     9121 acgatggcga gaacaagatc accggcctga actaccggat catgcagagc gccattgccg
     9181 tgtacgatag cggcgacgat tacgaggcca agcagcagtt caagctgatg ttcgagaagg
     9241 cccggctgat cggcaagggc acaacagagc ctcatccatt tctgtacaag gtgttcgccc
     9301 gcagcatccc cgccaatgcc gtcgagttct acgagcgcta cctgatcgag cggaagttct
     9361 acctgaccgg cctgtccaac gagatcaaga aaggcaacag agtggatgtg cccttcatcc
     9421 ggcgggacca gaacaagtgg aaaacacccg ccatgaagac cctgggcaga atctacagcg
     9481 aggatctgcc cgtggaactg cccagacaga tgttcgacaa tgagatcaag tcccacctga
     9541 agtccctgcc acagatggaa ggcatcgact tcaacaatgc caacgtgacc tatctgatcg
     9601 ccgagtacat gaagagagtg ctggacgacg acttccagac cttctaccag tggaaccgca
     9661 actaccggta catggacatg cttaagggcg agtacgacag aaagggctcc ctgcagcact
     9721 gcttcaccag cgtggaagag agagaaggcc tctggaaaga gcgggcctcc agaacagagc
     9781 ggtacagaaa gcaggccagc aacaagatcc gcagcaaccg gcagatgaga aacgccagca
     9841 gcgaagagat cgagacaatc ctggataagc ggctgagcaa cagccggaac gagtaccaga
     9901 aaagcgagaa agtgatccgg cgctacagag tgcaggatgc cctgctgttt ctgctggcca
     9961 aaaagaccct gaccgaactg gccgatttcg acggcgagag gttcaaactg aaagaaatca
    10021 tgcccgacgc cgagaaggga atcctgagcg agatcatgcc catgagcttc accttcgaga
    10081 aaggcggcaa gaagtacacc atcaccagcg agggcatgaa gctgaagaac tacggcgact
    10141 tctttgtgct ggctagcgac aagaggatcg gcaacctgct ggaactcgtg ggcagcgaca
    10201 tcgtgtccaa agaggatatc atggaagagt tcaacaaata cgaccagtgc aggcccgaga
    10261 tcagctccat cgtgttcaac ctggaaaagt gggccttcga cacatacccc gagctgtctg
    10321 ccagagtgga ccgggaagag aaggtggact tcaagagcat cctgaaaatc ctgctgaaca
    10381 acaagaacat caacaaagag cagagcgaca tcctgcggaa gatccggaac gccttcgatc
    10441 acaacaatta ccccgacaaa ggcgtggtgg aaatcaaggc cctgcctgag atcgccatga
    10501 gcatcaagaa ggcctttggg gagtacgcca tcatgaaggg atcccttcaa ctgcctccac
    10561 ttgaaagact gacactggac tataaggacc acgacggaga ctacaaggat catgatattg
    10621 attacaaaga cgatgacgat aagggcggcg ggtccggagg agagggcaga ggaagtctcc
    10681 taacatgcgg tgacgtggag gagaatcctg gcccaatgag cgagctgatt aaggagaaca
    10741 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
    10801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
    10861 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
    10921 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
    10981 catgggagag agtcaccaca tacgaggacg ggggcgtgct gaccgctacc caggacacca
    11041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
    11101 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag accctgtacc
    11161 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
    11221 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
    11281 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
    11341 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
    11401 ggcacaagct taattgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
    11461 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    11521 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    11581 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    11641 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    11701 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    11761 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    11821 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    11881 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    11941 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    12001 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
    12061 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
    12121 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
    12181 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
    12241 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    12301 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
    12361 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
    12421 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    12481 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
    12541 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    12601 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    12661 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    12721 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
    12781 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    12841 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    12901 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    12961 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    13021 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    13081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    13141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    13201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    13261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    13321 gttctttaat agtggactct tgttccaaac tg
//
