LOCUS       sequence-341545-       10692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA-dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H
  TITLE     Background-suppressed live visualization of genomic loci with an 
            improved CRISPR system based on a split fluorophore.
  JOURNAL   Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. 
            Epub 2020 Sep 4.
  PUBMED    32887690
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.260018.119"; journalName: "Genome Res"; date: "2020-09";
            volume: "30"; issue: "9"; pages: "1306-1316"
COMMENT     Sequence Label: pM80
FEATURES             Location/Qualifiers
     source          1..10692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2238..2617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2618..2821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2772..2792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2849..2875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2882..2902
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2903..2926,2927..2929,2930..5416,5417..5419,
                     5420..7003)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7013..7033
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7082..7670
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7135..7155)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7553..7564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7745..7978
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8097..8115)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8183..8287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8288..8356,8357..9148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8506..8525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9319..9907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9808..9827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10061..10078
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10318..10339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10354..10359,10360..10377,10378..10384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10392..10408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10397..10419
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10416..10432
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10416..10432
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10482..10501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
     2221 gctagactcg aggcgttgac attgattatt gactagttat taatagtaat caattacggg
     2281 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2341 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2401 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2461 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2521 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2581 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2641 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2701 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2761 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2821 tctctggcta actaccggtg ccaccatgta cccatacgat gttccagatt acgctgctag
     2881 ccccaaaaag aagaggaaag tggacaagaa gtattctatc ggactggcca tcgggactaa
     2941 tagcgtcggg tgggccgtga tcactgacga gtacaaggtg ccctctaaga agttcaaggt
     3001 gctcgggaac accgaccggc attccatcaa gaaaaatctg atcggagctc tcctctttga
     3061 ttcaggggag accgctgaag caacccgcct caagcggact gctagacggc ggtacaccag
     3121 gaggaagaac cggatttgtt accttcaaga gatattctcc aacgaaatgg caaaggtcga
     3181 cgacagcttc ttccataggc tggaagaatc attcctcgtg gaagaggata agaagcatga
     3241 acggcatccc atcttcggta atatcgtcga cgaggtggcc tatcacgaga aatacccaac
     3301 catctaccat cttcgcaaaa agctggtgga ctcaaccgac aaggcagacc tccggcttat
     3361 ctacctggcc ctggcccaca tgatcaagtt cagaggccac ttcctgatcg agggcgacct
     3421 caatcctgac aatagcgatg tggataaact gttcatccag ctggtgcaga cttacaacca
     3481 gctctttgaa gagaacccca tcaatgcaag cggagtcgat gccaaggcca ttctgtcagc
     3541 ccggctgtca aagagccgca gacttgagaa tcttatcgct cagctgccgg gtgaaaagaa
     3601 aaatggactg ttcgggaacc tgattgctct ttcacttggg ctgactccca atttcaagtc
     3661 taatttcgac ctggcagagg atgccaagct gcaactgtcc aaggacacct atgatgacga
     3721 tctcgacaac ctcctggccc agatcggtga ccaatacgcc gaccttttcc ttgctgctaa
     3781 gaatctttct gacgccatcc tgctgtctga cattctccgc gtgaacactg aaatcaccaa
     3841 ggcccctctt tcagcttcaa tgattaagcg gtatgatgag caccaccagg acctgaccct
     3901 gcttaaggca ctcgtccggc agcagcttcc ggagaagtac aaggaaatct tctttgacca
     3961 gtcaaagaat ggatacgccg gctacatcga cggaggtgcc tcccaagagg aattttataa
     4021 gtttatcaaa cctatccttg agaagatgga cggcaccgaa gagctcctcg tgaaactgaa
     4081 tcgggaggat ctgctgcgga agcagcgcac tttcgacaat gggagcattc cccaccagat
     4141 ccatcttggg gagcttcacg ccatccttcg gcgccaagag gacttctacc cctttcttaa
     4201 ggacaacagg gagaagattg agaaaattct cactttccgc atcccctact acgtgggacc
     4261 cctcgccaga ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag aaactatcac
     4321 tccctggaac ttcgaagagg tggtggacaa gggagccagc gctcagtcat tcatcgaacg
     4381 gatgactaac ttcgataaga acctccccaa tgagaaggtc ctgccgaaac attccctgct
     4441 ctacgagtac tttaccgtgt acaacgagct gaccaaggtg aaatatgtca ccgaagggat
     4501 gaggaagccc gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa
     4561 gaccaataga aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg
     4621 cttcgactct gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta
     4681 ccatgatctg cttaagatca tcaaggacaa ggattttctg gacaatgagg agaacgagga
     4741 catccttgag gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga
     4801 gaggcttaag acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag
     4861 aagatatacc ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca
     4921 gagcggaaag accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat
     4981 gcaacttatc catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc
     5041 tggacaaggt gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa
     5101 gaagggtatt ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca
     5161 taaaccagag aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca
     5221 gaagaacagc agggagcgga tgaaaagaat tgaggaaggg attaaggagc tcgggtcaca
     5281 gatccttaaa gagcacccgg tggaaaacac ccagcttcag aatgagaagc tctatctgta
     5341 ctaccttcaa aatggacgcg atatgtatgt ggaccaagag cttgatatca acaggctctc
     5401 agactacgac gtggacgcca tcgtccctca gagcttcctc aaagacgact caattgacaa
     5461 taaggtgctg actcgctcag acaagaaccg gggaaagtca gataacgtgc cctcagagga
     5521 agtcgtgaaa aagatgaaga actattggcg ccagcttctg aacgcaaagc tgatcactca
     5581 gcggaagttc gacaatctca ctaaggctga gaggggcgga ctgagcgaac tggacaaagc
     5641 aggattcatt aaacggcaac ttgtggagac tcggcagatt actaaacatg tcgcccaaat
     5701 ccttgactca cgcatgaata ccaagtacga cgaaaacgac aaacttatcc gcgaggtgaa
     5761 ggtgattacc ctgaagtcca agctggtcag cgatttcaga aaggactttc aattctacaa
     5821 agtgcgggag atcaataact atcatcatgc tcatgacgca tatctgaatg ccgtggtggg
     5881 aaccgccctg atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg gagactacaa
     5941 ggtctacgac gtgcgcaaga tgattgccaa atctgagcag gagatcggaa aggccaccgc
     6001 aaagtacttc ttctacagca acatcatgaa tttcttcaag accgaaatca cccttgcaaa
     6061 cggtgagatc cggaagaggc cgctcatcga gactaatggg gagactggcg aaatcgtgtg
     6121 ggacaagggc agagatttcg ctaccgtgcg caaagtgctt tctatgcctc aagtgaacat
     6181 cgtgaagaaa accgaggtgc aaaccggagg cttttctaag gaatcaatcc tccccaagcg
     6241 caactccgac aagctcattg caaggaagaa ggattgggac cctaagaagt acggcggatt
     6301 cgattcacca actgtggctt attctgtcct ggtcgtggct aaggtggaaa aaggaaagtc
     6361 taagaagctc aagagcgtga aggaactgct gggtatcacc attatggagc gcagctcctt
     6421 cgagaagaac ccaattgact ttctcgaagc caaaggttac aaggaagtca agaaggacct
     6481 tatcatcaag ctcccaaagt atagcctgtt cgaactggag aatgggcgga agcggatgct
     6541 cgcctccgct ggcgaacttc agaagggtaa tgagctggct ctcccctcca agtacgtgaa
     6601 tttcctctac cttgcaagcc attacgagaa gctgaagggg agccccgagg acaacgagca
     6661 aaagcaactg tttgtggagc agcataagca ttatctggac gagatcattg agcagatttc
     6721 cgagttttct aaacgcgtca ttctcgctga tgccaacctc gataaagtcc ttagcgcata
     6781 caataagcac agagacaaac caattcggga gcaggctgag aatatcatcc acctgttcac
     6841 cctcaccaat cttggtgccc ctgccgcatt caagtacttc gacaccacca tcgaccggaa
     6901 acgctatacc tccaccaaag aagtgctgga cgccaccctc atccaccaga gcatcaccgg
     6961 actttacgaa actcggattg acctctcaca gctcggaggg gatgagggag ctcccaagaa
     7021 aaagcgcaag gtaggtagtt cctaagcggc cgctctggct ctcgagtagg gtaatctaga
     7081 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7141 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7201 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7261 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7321 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7381 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7441 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7501 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7561 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7621 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     7681 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7741 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     7801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7981 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8041 agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     8101 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8161 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8221 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8281 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8341 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8401 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8461 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8521 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8581 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8641 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8701 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8761 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8821 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8881 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8941 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9001 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9061 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9121 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9181 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9241 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9421 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9961 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10021 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10081 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10141 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10201 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10261 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10321 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    10381 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
    10441 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10501 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    10561 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    10621 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    10681 aaacttagta gt
//
