LOCUS       sequence-341552-        7920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  (Empty Backbone) gRNA-12xMBS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H
  TITLE     Background-suppressed live visualization of genomic loci with an 
            improved CRISPR system based on a split fluorophore.
  JOURNAL   Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. 
            Epub 2020 Sep 4.
  PUBMED    32887690
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.260018.119"; journalName: "Genome Res"; date: "2020-09";
            volume: "30"; issue: "9"; pages: "1306-1316"
COMMENT     Sequence Label: pM370
FEATURES             Location/Qualifiers
     source          1..7920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    157..390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             575..619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        917..1165
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     917..937
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1088..1107
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1207..1225
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1236..1254
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1294..1312
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1512..1530
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    1609..1726
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1775..2285
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2204..2222
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2375..3400
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2375..3400
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(3424..3440)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3425..3441)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3523..3756
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3828..3949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3865..3884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3919..3938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3989..4124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4144..4163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4145..4163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4173..4190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4173..4189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4182..4204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4331..4786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4418..4437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4628..4649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4812..4916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4917..4985,4986..5777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5135..5154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5948..6536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6437..6456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6690..6707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6824..6845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6860..6865,6866..6883,6884..6890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6898..6914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6903..6925
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6922..6938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6922..6938
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6957..6977
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6959..6977
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7005..7231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7232..7412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7459..7584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
       61 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
      121 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
      181 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
      241 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
      301 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
      361 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
      421 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
      481 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
      541 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
      601 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
      661 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
      721 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
      781 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
      841 cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc
      901 ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata
      961 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
     1021 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     1081 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     1141 tatcttgtgg aaaggacgaa acaccggtgt cttcgagaag acctgtttga gagctaggcc
     1201 gggagcacat gaggatcacc catgtgccac gagcgacatg aggatcaccc atgtcgctcg
     1261 tgttccctct tcgtagagga tcaccctacg tctacatgag gatcacccat gttctggtag
     1321 aggatcaccc tacctctcgt agaggatcac cctacgtctg caggaggatc acccctgctc
     1381 tcgaggagga tcacccctcg tctgactgag gatcacccag tctctgctag aggatcaccc
     1441 tagctctcct tgaggatcac ccaaggggcc tagcaagttc aaataaggct agtccgttat
     1501 caacttggcc aacatgagga tcacccatgt ctgcagggcc aagtggcacc gagtcggtgc
     1561 tttttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1621 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1681 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1741 ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag
     1801 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     1861 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     1921 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     1981 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2041 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2101 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2161 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2221 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2281 cccaggggga tccaccggat caagcttgcc agctggggcg ccctctggta aggttgggaa
     2341 gccctgcaga tctgatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     2401 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     2461 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     2521 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     2581 ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta
     2641 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagat ctgcctgaaa ccgaactgcc
     2701 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     2761 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     2821 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     2881 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     2941 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3001 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3061 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3121 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3181 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     3241 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     3301 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     3361 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgggatc
     3421 tatcgatacc gtcgacctcg agggggggcc cggtaccttt aagaccaatg acttacaagg
     3481 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3541 cccaacgaag aaaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3601 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3661 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3721 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     3781 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3961 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4141 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4201 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4261 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4321 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6781 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6841 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6901 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6961 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7021 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7081 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7141 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7201 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7261 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7321 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7381 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7441 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7501 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7561 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7621 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7681 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7741 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7801 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7861 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
//
