LOCUS       sequence-341619-        8050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, 
            CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Wang X, Friesen E, Muller I, Lemieux M, Dukart R, Maia IB, Kalia S, 
            Schmitt-Ulms G
  TITLE     Rapid Generation of Human Neuronal Cell Models Enabling Inducible 
            Expression of Proteins-of-interest for Functional Studies.
  JOURNAL   Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. 
            eCollection 2020 May 5.
  PUBMED    33659578
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2020-05-5"; volume: "10(9):e3615. doi"; pages: " 
            10.21769/BioProtoc.3615. eCollection 2020 May 5"
COMMENT     Sequence Label: AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP
FEATURES             Location/Qualifiers
     source          1..8050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(990..1023)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1026..1059
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    1064..1089
                     /label=SA
                     /note="splice acceptor site"
     CDS             1113..1166
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1176..1775
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1176..1195)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1672..1692
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    1778..1811
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    1814..2038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2045..2126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2264..2971)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     primer_bind     complement(3015..3034)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3096..3118)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     polyA_signal    3941..4062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3952..3971)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(4185..4898,4899..4901,4902..4904))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4188..4898,4899..4901,4902..4904))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4230..4251)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4579..4598
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4838..4859
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4901..4910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6278..6287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6319..6343)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6360..6674)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(6363..6387)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6434..6452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6470..6488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6505..6523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6541..6559
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6577..6595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6612..6630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6648..6666
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(6691..6724)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(join(7089..7880,7881..7949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7712..7731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaactgtc atggcatctt ccaggggtcc gagagctcag ctagtcttct tcctccaacc
      841 cgggccccta tgtccacttc aggacagcat gtttgctgcc tccagggatc ctgtgtcccc
      901 gagctgggac caccttatat tcccagggcc ggttaatgtg gctctggttc tgggtacttt
      961 tatctgtccc ctccacccca cagtggggca taacttcgta tagcatacat tatacgaacg
     1021 gtaaagaagt tcctattctc tagaaagtat aggaacttcg cttctgacct cttctcttcc
     1081 tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt ctaacatgcg
     1141 gtgacgtgga ggagaatccc ggccctaggc tcgagatgac cgagtacaag cccacggtgc
     1201 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     1261 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     1321 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     1381 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     1441 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     1501 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     1561 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     1621 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     1681 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     1741 gcacctggtg catgacccgc aagcccggtg cctgatcgaa gttcctattc tctagaaagt
     1801 ataggaactt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1861 ccttccttga ccctggaaga tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1921 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1981 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt
     2041 cgaggcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2101 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2161 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2221 ctgattatga tcctctagag atatcgtcga caagcttatc gatttacccg gggagcatgt
     2281 caaggtcaaa atcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
     2341 cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     2401 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc
     2461 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     2521 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     2581 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     2641 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     2701 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
     2761 ccagggggca gaagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     2821 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
     2881 cgagtttcct tgtcgtcagg ccttcgatac cgacaccatt gagtaattcc agagctccgt
     2941 ttatgacttt gctcttgtcc agcctagaca tggtggcact agtgatccaa ttctttgcca
     3001 aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag
     3061 gcatgaacat ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc
     3121 gccctgcccc gtcccccccg aaggcagccg tccccccgcg gacagccccg agccgctccc
     3181 cccgcacaaa gggccctccc ggagcccctc aaggctttca cgcagccaca gaaaagaaac
     3241 aagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct
     3301 cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcggcgga
     3361 gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt
     3421 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
     3481 attggctgcc gccscmccyc yccsccycsc cccccccccc cccccccccc cccccccccc
     3541 ccccggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
     3601 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
     3661 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     3721 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     3781 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     3841 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     3901 atacgtcatt attgacgtca atggtacgat cttcctagct taagatacat tgatgagttt
     3961 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4021 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4081 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4141 tacaaatgtg gtatggctga ttatgatcta gagtcgcggc cgctttactt gtacagctcg
     4201 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     4261 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     4321 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     4381 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     4441 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     4501 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     4561 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     4621 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     4681 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     4741 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     4801 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     4861 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga
     4921 tcccgggccc gcggtaccgt cgactgcaga attcttatca aaccctgctt ggccagggag
     4981 gcagacacct cgtcagctag cgtggcgagc tggggcgagt ctaccatgtc gatgctgccg
     5041 gtggaggaga cattgctgag atgccgtgga gacgtgtccc cagacaccac tggcgacttg
     5101 tacacgatct ccgccccgtg gtctgtcttg gctttggcgt tctcgcggaa ggtcagcttg
     5161 tgggtttcaa tctttttatt tcctccgcca gggacgtggg tgatattgtc cagggaccca
     5221 atcttcgact ggactctgtc cttgaagtca agcttctcag attttacttc cacctggcca
     5281 cctcctggtt tatgatggat gttgcctaat gagccacact tggaggtcac cttgctcagg
     5341 tcaactggtt tgtagactat ttgcacactg ccgcctccca ggacgtgttt gatattatcc
     5401 tttgagccac acttggactg gacgttgcta agatccagct tcttattaat tatctgcacc
     5461 ttcccgcctc ccggctggtg cttcaggttc tcagtggagc cgatcttgga cttgacattc
     5521 ttcaggtctg gcatgggcac gggggctgtc tgcaggcggc tcttggcgga agacggcgac
     5581 ttgggtggag tacggaccac tgccaccttc ttgggctccc gggtgggtgg ggttggaagg
     5641 gacggggtgc gggagcggct gccgggagtg cctggggagc cggggctgct gtagccgctg
     5701 cgatcccctg attttggagg ttcaccagag ctgggtggtg tctttggagc gggcggggtt
     5761 tttgctggaa tcctggtggc gttggcctgg cccttctggc ctggaggggc tgctccccgc
     5821 ggtgtggcga tcttcgtttt accatcagcc cccttggctt ttttgtcatc gcttccagtc
     5881 ccgtctttgc ttttactgac catgcgagct tgggtcacgt gaccagcagc ttcgtcttcc
     5941 aggctggggg tgtctccaat gcctgcttct tcagctgtgg ttccttctgg gatctccgtg
     6001 tggggctgcg cggcagcctg cttgccggga gctccctcat ccactaaggg tgctgtcaca
     6061 tcttccgctg ttggagtgct cttagcatca gaggtttcag agcccggttc ctcagatccg
     6121 tcctcagtgg gggtctgcag gggagattct ttcaggccag cgtccgtgtc accctcttgg
     6181 tcttggtgca tggtgtagcc cccctgatct ttcctgtccc ccaacccgta cgtcccagcg
     6241 tgatcttcca tcacttcgaa ctcctggcgg ggctcagcca tggtggcgct gaggaccggt
     6301 ccaacctgaa aaccgcggag cctgcttttt tgtacaaact tgttgatcgg aattctccag
     6361 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccta
     6421 cctcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact
     6481 gatagggata aactcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
     6541 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa
     6601 ctcgacatcg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
     6661 tagggagtaa actcgacagt actaagcttt ataacttcgt ataggatact ttatacgaag
     6721 ttatactagg gacaggattg gtgacagaaa agccccatcc ttaggcctcc tccttcctag
     6781 tctcctgata ttgggtctaa cccccacctc ctgttaggca gattccttat ctggtgacac
     6841 acccccattt cctggagcca tctctctcct tgccagaacc tctaaggttt gcttacgatg
     6901 gagccagcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6961 ttctaaatac attcaaatat gtatccgctc atgagattat caaaaaggat cttcacctag
     7021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7621 tgcttttctg tgactggtga atactcaacc aagtcattct gagaatagtg tatgcggcga
     7681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7981 tatcagggtt attgtctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8041 tcagaccccg
//
