LOCUS       sequence-341638-       13749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich 
            A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, 
            Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, 
            Cookson MR
  TITLE     Kinase activity is required for the toxic effects of mutant 
            LRRK2/dardarin.
  JOURNAL   Neurobiol Dis. 2006 Aug . 23(2):329-41.
  PUBMED    16750377
REFERENCE   2  (bases 1 to 13749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2006 Aug . 23(2):329-41."
COMMENT     Sequence Label: pDEST53-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..13749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             112..906
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(166..185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     776..795
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1080..1201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1117..1136)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1171..1190
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1250..1266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1250..1266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1263..1285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1274..1290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1298..1304,1305..1322,1323..1328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1343..1364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1481..1498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1652..2240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1732..1751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2411..3202,3203..3271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3034..3053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3272..3376)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3451..3470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3642..4021
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4022..4225
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4176..4196
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4270..4289
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4270..4288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4312..4314,4315..4317,4318..5028)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4345..4373)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4968..4988
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    5050..5074
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12691..12715)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(12747..12764)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12753..12977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13023..13451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13110..13129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13320..13341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13460..13480)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(13465..13749,1..45)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13645..13749,1..31)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13707..13726
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
       61 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
      121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
      181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
      241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
      301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
      361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
      421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
      661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      781 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
      841 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      901 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
      961 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     1021 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     1081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1141 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1201 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     1261 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1321 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1381 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1441 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2281 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2341 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2401 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2521 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3361 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     3421 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3481 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     3541 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     3601 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     3661 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3721 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3781 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3841 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3901 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3961 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4021 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     4081 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     4141 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     4201 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     4261 tatcgaaatt aatacgactc actataggga gacccaagct ggctagacac catggccagc
     4321 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     4381 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgctacata cggaaagctt
     4441 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     4501 actttctctt atggtgttca atgcttttcc cgttatccgg atcatatgaa acggcatgac
     4561 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     4621 gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     4681 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca caaactcgag
     4741 tacaactata actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     4801 gctaacttca aaattcgcca caacattgaa gatggatccg ttcaactagc agaccattat
     4861 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     4921 acacaatctg ccctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     4981 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaaag cggttccgga
     5041 ccggatcaaa caagtttgta caaaaaagca ggctccgaat tcgcccttat ggctagtggc
     5101 agctgtcagg ggtgcgaaga ggacgaggaa actctgaaga agttgatagt caggctgaac
     5161 aatgtccagg aaggaaaaca gatagaaacg ctggtccaaa tcctggagga tctgctggtg
     5221 ttcacgtact ccgagcacgc ctccaagtta tttcaaggca aaaatatcca tgtgcctctg
     5281 ttgatcgtct tggactccta tatgagagtc gcgagtgtgc agcaggtggg ttggtcactt
     5341 ctgtgcaaat taatagaagt ctgtccaggt acaatgcaaa gcttaatggg accccaggat
     5401 gttggaaatg attgggaagt ccttggtgtt caccaattga ttcttaaaat gctaacagtt
     5461 cataatgcca gtgtaaactt gtcagtgatt ggactgaaga ccttagatct cctcctaact
     5521 tcaggtaaaa tcaccttgct gatattggat gaagaaagtg atattttcat gttaattttt
     5581 gatgccatgc actcatttcc agccaatgat gaagtccaga aacttggatg caaagcttta
     5641 catgtgctgt ttgagagagt ctcagaggag caactgactg aatttgttga gaacaaagat
     5701 tatatgatat tgttaagtgc gttaacaaat tttaaagatg aagaggaaat tgtgcttcat
     5761 gtgctgcatt gtttacattc cctagcgatt ccttgcaata atgtggaagt cctcatgagt
     5821 ggcaatgtca ggtgttataa tattgtggtg gaagctatga aagcattccc tatgagtgaa
     5881 agaattcaag aagtgagttg ctgtttgctc cataggctta cattaggtaa ttttttcaat
     5941 atcctggtat taaacgaagt ccatgagttt gtggtgaaag ctgtgcagca gtacccagag
     6001 aatgcagcat tgcagatctc agcgctcagc tgtttggccc tcctcactga gactattttc
     6061 ttaaatcaag atttagagga aaagaatgag aatcaagaga atgatgatga gggggaagaa
     6121 gataaattgt tttggctgga agcctgttac aaagcattaa cgtggcatag aaagaacaag
     6181 cacgtgcagg aggccgcatg ctgggcacta aataatctcc ttatgtacca aaacagttta
     6241 catgagaaga ttggagatga agatggccat ttcccagctc atagggaagt gatgctctcc
     6301 atgctgatgc attcttcatc aaaggaagtt ttccaggcat ctgcgaatgc attgtcaact
     6361 ctcttagaac aaaatgttaa tttcagaaaa atactgttat caaaaggaat acacctgaat
     6421 gttttggagt taatgcagaa gcatatacat tctcccgaag tggctgaaag tggctgtaaa
     6481 atgctaaatc atctttttga aggaagcaac acttccctgg atataatggc agcagtggtc
     6541 cccaaaatac taacagttat gaaacgtcat gagacatcat taccagtgca gctggaggcg
     6601 cttcgagcta ttttacattt tatagtgcct ggcatgccag aagaatccag ggaggataca
     6661 gaatttcatc ataagctaaa tatggttaaa aaacagtgtt tcaagaatga tattcacaaa
     6721 ctggtcctag cagctttgaa caggttcatt ggaaatcctg ggattcagaa atgtggatta
     6781 aaagtaattt cttctattgt acattttcct gatgcattag agatgttatc cctggaaggt
     6841 gctatggatt cagtgcttca cacactgcag atgtatccag atgaccaaga aattcagtgt
     6901 ctgggtttaa gtcttatagg atacttgatt acaaagaaga atgtgttcat aggaactgga
     6961 catctgctgg caaaaattct ggtttccagc ttataccgat ttaaggatgt tgctgaaata
     7021 cagactaaag gatttcagac aatcttagca atcctcaaat tgtcagcatc tttttctaag
     7081 ctgctggtgc atcattcatt tgacttagta atattccatc aaatgtcttc caatatcatg
     7141 gaacaaaagg atcaacagtt tctaaacctc tgttgcaagt gttttgcaaa agtagctatg
     7201 gatgattact taaaaaatgt gatgctagag agagcgtgtg atcagaataa cagcatcatg
     7261 gttgaatgct tgcttctatt gggagcagat gccaatcaag caaaggaggg atcttcttta
     7321 atttgtcagg tatgtgagaa agagagcagt cccaaattgg tggaactctt actgaatagt
     7381 ggatctcgtg aacaagatgt acgaaaagcg ttgacgataa gcattgggaa aggtgacagc
     7441 cagatcatca gcttgctctt aaggaggctg gccctggatg ttgccaacaa tagcatttgc
     7501 cttggaggat tttgtatagg aaaagttgaa ccttcttggc ttggtccttt atttccagat
     7561 aagacttcta atttaaggaa acaaacaaat atagcatcta cactagcaag aatggtgatc
     7621 agatatcaga tgaaaagtgc tgtggaagaa ggaacagcct caggcagcga tggaaatttt
     7681 tctgaagatg tgctgtctaa atttgatgaa tggaccttta ttcctgactc ttctatggac
     7741 agtgtgtttg ctcaaagtga tgacctggat agtgaaggaa gtgaaggctc atttcttgtg
     7801 aaaaagaaat ctaattcaat tagtgtagga gaattttacc gagatgccgt attacagcgt
     7861 tgctcaccaa atttgcaaag acattccaat tccttggggc ccatttttga tcatgaagat
     7921 ttactgaagc gaaaaagaaa aatattatct tcagatgatt cactcaggtc atcaaaactt
     7981 caatcccata tgaggcattc agacagcatt tcttctctgg cttctgagag agaatatatt
     8041 acatcactag acctttcagc aaatgaacta agagatattg atgccctaag ccagaaatgc
     8101 tgtataagtg ttcatttgga gcatcttgaa aagctggagc ttcaccagaa tgcactcacg
     8161 agctttccac aacagctatg tgaaactctg aagagtttga cacatttgga cttgcacagt
     8221 aataaattta catcatttcc ttcttatttg ttgaaaatga gttgtattgc taatcttgat
     8281 gtctctcgaa atgacattgg accctcagtg gttttagatc ctacagtgaa atgtccaact
     8341 ctgaaacagt ttaacctgtc atataaccag ctgtcttttg tacctgagaa cctcactgat
     8401 gtggtagaga aactggagca gctcatttta gaaggaaata aaatatcagg gatatgctcc
     8461 cccttgagac tgaaggaact gaagatttta aaccttagta agaaccacat ttcatcccta
     8521 tcagagaact ttcttgaggc ttgtcctaaa gtggagagtt tcagtgccag aatgaatttt
     8581 cttgctgcta tgcctttctt gcctccttct atgacaatcc taaaattatc tcagaacaaa
     8641 ttttcctgta ttccagaagc aattttaaat cttccacact tgcggtcttt agatatgagc
     8701 agcaatgata ttcagtacct accaggtccc gcacactgga aatctttgaa cttaagggaa
     8761 ctcttattta gccataatca gatcagcatc ttggacttga gtgaaaaagc atatttatgg
     8821 tctagagtag agaaactgca tctttctcac aataaactga aagagattcc tcctgagatt
     8881 ggctgtcttg aaaatctgac atctctggat gtcagttaca acttggaact aagatccttt
     8941 cccaatgaaa tggggaaatt aagcaaaata tgggatcttc ctttggatga actgcatctt
     9001 aactttgatt ttaaacatat aggatgtaaa gccaaagaca tcataaggtt tcttcaacag
     9061 cgattaaaaa aggctgtgcc ttataaccga atgaaactta tgattgtggg aaatactggg
     9121 agtggtaaaa ccaccttatt gcagcaatta atgaaaacca agaaatcaga tcttggaatg
     9181 caaagtgcca cagttggcat agatgtgaaa gactggccta tccaaataag agacaaaaga
     9241 aagagagatc tcgtcctaaa tgtgtgggat tttgcaggtc gtgaggaatt ctatagtact
     9301 catccccatt ttatgacgca gcgagcattg taccttgctg tctatgacct cagcaaggga
     9361 caggctgaag ttgatgccat gaagccttgg ctcttcaata taaaggctcg cgcttcttct
     9421 tcccctgtga ttctcgttgg cacacatttg gatgtttctg atgagaagca acgcaaagcc
     9481 tgcatgagta aaatcaccaa ggaactcctg aataagcgag ggttccctgc catacgagat
     9541 taccactttg tgaatgccac cgaggaatct gatgctttgg caaaacttcg gaaaaccatc
     9601 ataaacgaga gccttaattt caagatccga gatcagcttg ttgttggaca gctgattcca
     9661 gactgctatg tagaacttga aaaaatcatt ttatcggagc gtaaaaatgt gccaattgaa
     9721 tttcccgtaa ttgaccggaa acgattatta caactagtga gagaaaatca gctgcagtta
     9781 gatgaaaatg agcttcctca cgcagttcac tttctaaatg aatcaggagt ccttcttcat
     9841 tttcaagacc cagcactgca gttaagtgac ttgtactttg tggaacccaa gtggctttgt
     9901 aaaatcatgg cacagatttt gacagtgaaa gtggaaggtt gtccaaaaca ccctaaggga
     9961 attatttcgc gtagagatgt ggaaaaattt ctttcaaaaa aaaggaaatt tccaaagaac
    10021 tacatgtcac agtattttaa gctcctagaa aaattccaga ttgctttgcc aataggagaa
    10081 gaatatttgc tggttccaag cagtttgtct gaccacaggc ctgtgataga gcttccccat
    10141 tgtgagaact ctgaaattat catccgacta tatgaaatgc cttattttcc aatgggattt
    10201 tggtcaagat taatcaatcg attacttgag atttcacctt acatgctttc agggagagaa
    10261 cgagcacttc gcccaaacag aatgtattgg cgacaaggca tttacttaaa ttggtctcct
    10321 gaagcttatt gtctggtagg atctgaagtc ttagacaatc atccagagag tttcttaaaa
    10381 attacagttc cttcttgtag aaaaggctgt attcttttgg gccaagttgt ggaccacatt
    10441 gattctctca tggaagaatg gtttcctggg ttgctggaga ttgatatttg tggtgaagga
    10501 gaaactctgt tgaagaaatg ggcattatat agttttaatg atggcgaaga acatcaaaaa
    10561 atcttacttg atgacttgat gaagaaagca gaggaaggag atctcttagt aaatccagat
    10621 caaccaaggc tcaccattcc aatatctcag attgcccctg acttgatttt ggctgacctg
    10681 cctagaaata ttatgttgaa taatgatgag ttggaatttg aacaagctcc agagtttctc
    10741 ctaggtgatg gcagttttgg atcagtttac cgagcagcct atgaaggaga agaagtggct
    10801 gtgaagattt ttaataaaca tacatcactc aggctgttaa gacaagagct tgtggtgctt
    10861 tgccacctcc accaccccag tttgatatct ttgctggcag ctgggattcg tccccggatg
    10921 ttggtgatgg agttagcctc caagggttcc ttggatcgcc tgcttcagca ggacaaagcc
    10981 agcctcacta gaaccctaca gcacaggatc gcactccacg tagctgatgg tttgagatac
    11041 ctccactcag ccatgattat ataccgagac ctgaaacccc acaatgtgct gcttttcaca
    11101 ctgtatccca atgctgccat catcgcaaag attgctgact acggcattgc tcagtactgc
    11161 tgtagaatgg ggataaaaac atcagagggc acaccagggt ttcgtgcacc tgaagttgcc
    11221 agaggaaatg tcatttataa ccaacaggct gatgtttatt catttggttt actactctat
    11281 gacattttga caactggagg tagaatagta gagggtttga agtttccaaa tgagtttgat
    11341 gaattagaaa tacaaggaaa attacctgat ccagttaaag aatatggttg tgccccatgg
    11401 cctatggttg agaaattaat taaacagtgt ttgaaagaaa atcctcaaga aaggcctact
    11461 tctgcccagg tctttgacat tttgaattca gctgaattag tctgtctgac gagacgcatt
    11521 ttattaccta aaaacgtaat tgttgaatgc atggttgcta cacatcacaa cagcaggaat
    11581 gcaagcattt ggctgggctg tgggcacacc gacagaggac agctctcatt tcttgactta
    11641 aatactgaag gatacacttc tgaggaagtt gctgatagta gaatattgtg cttagccttg
    11701 gtgcatcttc ctgttgaaaa ggaaagctgg attgtgtctg ggacacagtc tggtactctc
    11761 ctggtcatca ataccgaaga tgggaaaaag agacataccc tagaaaagat gactgattct
    11821 gtcacttgtt tgtattgcaa ttccttttcc aagcaaagca aacaaaaaaa ttttcttttg
    11881 gttggaaccg ctgatggcaa gttagcaatt tttgaagata agactgttaa gcttaaagga
    11941 gctgctcctt tgaagatact aaatatagga aatgtcagta ctccattgat gtgtttgagt
    12001 gaatccacaa attcaacgga aagaaatgta atgtggggag gatgtggcac aaagattttc
    12061 tccttttcta atgatttcac cattcagaaa ctcattgaga caagaacaag ccaactgttt
    12121 tcttatgcag ctttcagtga ttccaacatc ataacagtgg tggtagacac tgctctctat
    12181 attgctaagc aaaatagccc tgttgtggaa gtgtgggata agaaaactga aaaactctgt
    12241 ggactaatag actgcgtgca ctttttaagg gaggtaatgg taaaagaaaa caaggaatca
    12301 aaacacaaaa tgtcttattc tgggagagtg aaaaccctct gccttcagaa gaacactgct
    12361 ctttggatag gaactggagg aggccatatt ttactcctgg atctttcaac tcgtcgactt
    12421 atacgtgtaa tttacaactt ttgtaattcg gtcagagtca tgatgacagc acagctagga
    12481 agccttaaaa atgtcatgct ggtattgggc tacaaccgga aaaatactga aggtacacaa
    12541 aagcagaaag agatacaatc ttgcttgacc gtttgggaca tcaatcttcc acatgaagtg
    12601 caaaatttag aaaaacacat tgaagtgaga aaagaattag ctgaaaaaat gagacgaaca
    12661 tctgttgagt aagagagaag ggcgaattcg acccagcttt cttgtacaaa gtggtgataa
    12721 ttaattaaga taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
    12781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
    12841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
    12901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
    12961 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
    13021 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    13081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    13141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    13201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    13261 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    13321 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    13381 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    13441 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    13501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    13561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    13621 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    13681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    13741 gagctattc
//
