LOCUS       sequence-341639-       11852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of MARK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11852)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-MARK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..66
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             135..734
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(135..154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     631..651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             872..1105
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1177..1298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1214..1233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1268..1287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1338..1473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1493..1512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1494..1512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1522..1539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1522..1538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1531..1553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1680..2135
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1767..1786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1977..1998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2161..2265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2266..2334,2335..3126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3297..3885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3786..3805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4039..4056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4173..4194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4209..4214,4215..4232,4233..4239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4247..4263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4252..4274
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4271..4287
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4271..4287
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4306..4326
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4308..4326
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4354..4580
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4581..4761
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4808..4933
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5426..5659
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5844..5888
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6155..6271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6305..6325
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6325..7536
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7373..7392
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7540..7560)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7561..7570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7573..8343
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8350..8391
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8392..8416
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10811..10835)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10865..11453
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10918..10938)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11336..11347)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(11467..11852,1..114)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11502..11525)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
       61 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      121 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      181 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      241 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      301 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      361 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      421 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      481 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      541 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
      601 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
      661 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
      721 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
      781 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
      841 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
      901 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
      961 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1021 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1081 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     1141 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     1201 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1261 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     1321 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1441 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     1501 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     1681 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1741 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1801 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1861 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1921 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1981 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2041 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2101 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     2161 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2221 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2281 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2341 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2401 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2461 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2521 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2581 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2641 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2701 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2761 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2821 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2881 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2941 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3001 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3061 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3181 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3961 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4021 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4081 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4141 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     4201 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     4261 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     4321 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     4381 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     4441 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     4501 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     4561 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     4621 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4681 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4741 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     4801 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     4861 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     4921 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     4981 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     5041 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     5101 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     5161 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     5221 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     5281 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     5341 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     5401 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     5461 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     5521 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     5581 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     5641 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     5701 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     5761 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     5821 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     5881 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     5941 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     6001 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     6061 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     6121 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     6181 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     6241 tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat
     6301 atcacaactt tgtatagaaa agttggcctc cgcgccgggt tttggcgcct cccgcgggcg
     6361 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     6421 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     6481 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     6541 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     6601 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     6661 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     6721 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     6781 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     6841 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     6901 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     6961 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     7021 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     7081 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     7141 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     7201 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     7261 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     7321 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     7381 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     7441 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     7501 gctaaattct ggccgttttt ggcttttttg ttagacggcc aacttttgta tacaaagtgg
     7561 gccaccatgg gaatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg
     7621 agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga tcgaatcggc
     7681 gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga
     7741 gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg
     7801 aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg
     7861 ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag
     7921 gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag
     7981 atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat
     8041 cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct
     8101 atgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca
     8161 tatctgtcac ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt
     8221 ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg
     8281 gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa
     8341 aaggctagcg gcaagcccat ccccaacccc ctgctgggcc tggacagcac cacaagtttg
     8401 tacaaaaaag caggctccac catgtcggcc cggaagccat tgccgacggt gaacgagcgg
     8461 gacacggaaa atcatacatc tgtggatgga tatactgaac cacacatcca gcctaccaag
     8521 tcgagtagca gacagaacat cccccggtgt agaaactcca ttacgtcagc aacagatgaa
     8581 cagcctcaca ttggaaatta ccgtttacaa aaaacaatag ggaagggaaa ttttgccaaa
     8641 gtcaaattgg caagacacgt tctaactggt agagaggttg ctgtgaaaat aatagacaaa
     8701 actcagctaa atcctaccag tctacaaaag ttatttcgag aagtacgaat aatgaagata
     8761 ctgaatcatc ctaatatagt aaaattgttt gaagttattg aaacagagaa gactctctat
     8821 ttagtcatgg aatacgcgag tgggggtgaa gtatttgatt acttagttgc ccatggaaga
     8881 atgaaagaga aagaggcccg tgcaaaattt aggcagattg tatctgctgt acagtattgt
     8941 catcaaaagt acattgttca ccgtgatctt aaggctgaaa accttctcct tgatggtgat
     9001 atgaatatta aaattgctga ctttggtttt agtaatgaat ttacagttgg gaacaaattg
     9061 gacacatttt gtggaagccc accctatgct gctcccgagc ttttccaagg aaagaagtat
     9121 gatgggcctg aagtggatgt gtggagtctg ggcgtcattc tctatacatt agtcagtggc
     9181 tccttgcctt tcgatggcca gaatttaaag gaactgcgag agcgagtttt acgagggaag
     9241 taccgtattc ccttctatat gtccacagac tgtgaaaatc ttctgaagaa attattagtc
     9301 ctgaatccaa taaagagagg cagcttggaa caaataatga aagatcgatg gatgaatgtt
     9361 ggtcatgaag aggaagaact aaagccatat actgagcctg atccggattt caatgacaca
     9421 aaaagaatag acattatggt caccatgggc tttgcacgag atgaaataaa tgatgcctta
     9481 ataaatcaga agtatgatga agttatggct acttatattc ttctaggtag aaaaccacct
     9541 gaatttgaag gtggtgaatc gttatccagt ggaaacttgt gtcagaggtc ccggcccagt
     9601 agtgacttaa acaacagcac tcttcagtcc cctgctcacc tgaaggtcca gagaagtatc
     9661 tcagcaaatc agaagcagcg gcgtttcagt gatcatgctg gtccatccat tcctcctgct
     9721 gtatcatata ccaaaagacc tcaggctaac agtgtggaaa gtgaacagaa agaggagtgg
     9781 gacaaagatg tggctcgaaa acttggcagc acaacagttg gatcaaaaag cgagatgact
     9841 gcaagccctc ttgtagggcc agagaggaaa aaatcttcaa ctattccaag taacaatgtg
     9901 tattctggag gtagcatggc aagaaggaat acatatgtct gtgaaaggac cacagatcga
     9961 tacgtagcat tgcagaatgg aaaagacagc agccttacgg agatgtctgt gagtagcata
    10021 tcttctgcag gctcttctgt ggcctctgct gtcccctcag cacgaccccg ccaccagaag
    10081 tccatgtcca cttctggtca tcctattaaa gtcacactgc caaccattaa agacggctct
    10141 gaagcttacc ggcctggtac aacccagaga gtgcctgctg cttccccatc tgctcacagt
    10201 attagtactg cgactccaga ccggacccgt tttccccgag ggagctcaag ccgaagcact
    10261 ttccatggtg aacagctccg ggagcgacgc agcgttgctt ataatgggcc acctgcttca
    10321 ccatcccatg aaacgggtgc atttgcacat gccagaaggg gaacgtcaac tggtataata
    10381 agcaaaatca catccaaatt tgttcgcagg gatccaagtg aaggcgaagc cagtggcaga
    10441 accgacacct caagaagtac atcaggggaa ccaaaagaaa gagacaagga agagggtaaa
    10501 gattctaagc cgcgttcttt gcggttcaca tggagtatga agaccactag ttcaatggac
    10561 cctaatgaca tgatgagaga aatccgaaaa gtgttagatg caaataactg tgattatgag
    10621 caaaaagaga gatttttgct tttctgtgtc catggagacg ctagacagga tagcctcgtg
    10681 cagtgggaga tggaagtctg caagttgcca cgactgtcac ttaatggggt tcgcttcaag
    10741 cgaatatctg ggacatctat tacctttaag aacattgcat caaaaatagc aaatgagctt
    10801 aagctgtagg acccagcttt cttgtacaaa gtggtgatat ccagcacagt ggcggccgct
    10861 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    10921 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    10981 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    11041 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    11101 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    11161 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    11221 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
    11281 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    11341 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    11401 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
    11461 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
    11521 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
    11581 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
    11641 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
    11701 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
    11761 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
    11821 gaaggggtgg gtccgggggc gggctcaggg gc
//
