LOCUS       sequence-341640-       11654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of MARK3 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11654)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-MARK3_IDG-K
FEATURES             Location/Qualifiers
     source          1..11654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    166..282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        336..1547
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1384..1403
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1551..1571)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1572..1581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1584..2354
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2361..2402
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2403..2427
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4624..4648)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4678..5266
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4731..4751)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5149..5160)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5280..5779
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5315..5338)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5712..5731
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5800..6399
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5800..5819)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6296..6316
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6537..6770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6842..6963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6879..6898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6933..6952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7003..7138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7158..7177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7159..7177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7187..7204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7187..7203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7196..7218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7345..7800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7432..7451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7642..7663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7826..7930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7931..7999,8000..8791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8962..9550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9451..9470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9704..9721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9838..9859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9874..9879,9880..9897,9898..9904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9912..9928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9917..9939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9936..9952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9936..9952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9971..9991
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9973..9991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10019..10245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10246..10426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10473..10598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11091..11324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11509..11553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
       61 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
      121 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
      181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
      241 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca gtgtggtgga
      301 attctgcaga tatcacaact ttgtatagaa aagttggcct ccgcgccggg ttttggcgcc
      361 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
      421 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
      481 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
      541 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
      601 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
      661 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
      721 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
      781 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
      841 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
      901 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
      961 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     1021 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     1081 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
     1141 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     1201 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     1261 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     1321 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     1381 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     1441 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     1501 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacggc caacttttgt
     1561 atacaaagtg ggccaccatg ggaatcccgc tgctgaacgc taaacagatt ctgggacagc
     1621 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     1681 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     1741 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     1801 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     1861 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     1921 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     1981 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     2041 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     2101 ctctggccgc tatgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     2161 gcctggctcc atatctgtca cggtgggaga agctggataa cttcatcaat agacccgtga
     2221 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     2281 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     2341 ggtctgccga aaaggctagc ggcaagccca tccccaaccc cctgctgggc ctggacagca
     2401 ccacaagttt gtacaaaaaa gcaggctcca ccatgtccac taggacccca ttgccaacgg
     2461 tgaatgaacg agacactgaa aaccacacgt cacatggaga tgggcgtcaa gaagttacct
     2521 ctcgtaccag ccgctcagga gctcggtgta gaaactctat agcctcctgt gcagatgaac
     2581 aacctcacat cggaaactac agactgttga aaacaatcgg caaggggaat tttgcaaaag
     2641 taaaattggc aagacatatc cttacaggca gagaggttgc aataaaaata attgacaaaa
     2701 ctcagttgaa tccaacaagt ctacaaaagc tcttcagaga agtaagaata atgaagattt
     2761 taaatcatcc caatatagtg aagttattcg aagtcattga aactgaaaaa acactctacc
     2821 taatcatgga atatgcaagt ggaggtgaag tatttgacta tttggttgca catggcagga
     2881 tgaaggaaaa agaagcaaga tctaaattta gacagattgt gtctgcagtt caatactgcc
     2941 atcagaaacg gatcgtacat cgagacctca aggctgaaaa tctattgtta gatgccgata
     3001 tgaacattaa aatagcagat ttcggtttta gcaatgaatt tactgttggc ggtaaactcg
     3061 acacgttttg tggcagtcct ccatacgcag cacctgagct cttccagggc aagaaatatg
     3121 acgggccaga agtggatgtg tggagtctgg gggtcatttt atacacacta gtcagtggct
     3181 cacttccctt tgatgggcaa aacctaaagg aactgagaga gagagtatta agagggaaat
     3241 acagaattcc cttctacatg tctacagact gtgaaaacct tctcaaacgt ttcctggtgc
     3301 taaatccaat taaacgcggc actctagagc aaatcatgaa ggacaggtgg atcaatgcag
     3361 ggcatgaaga agatgaactc aaaccatttg ttgaaccaga gctagacatc tcagaccaaa
     3421 aaagaataga tattatggtg ggaatgggat attcacaaga agaaattcaa gaatctctta
     3481 gtaagatgaa atacgatgaa atcacagcta catatttgtt attggggaga aaatcttcag
     3541 agctggatgc tagtgattcc agttctagca gcaatctttc acttgctaag gttaggccga
     3601 gcagtgatct caacaacagt actggccagt ctcctcacca caaagtgcag agaagtgttt
     3661 cttcaagcca aaagcaaaga cgctacagtg accatgctgg accagctatt ccttctgttg
     3721 tggcgtatcc gaaaaggagt cagaccagca ctgcagatag tgacctcaaa gaagatggaa
     3781 tttcctcccg gaaatcaagt ggcagtgctg ttggaggaaa gggaattgct ccagccagtc
     3841 ccatgcttgg gaatgcaagt aatcctaata aggcggatat tcctgaacgc aagaaaagct
     3901 ccactgtccc tagtagtaac acagcatctg gtggaatgac acgacgaaat acttatgttt
     3961 gcagtgagag aactacagct gatagacact cagtgattca gaatggcaaa gaaaacagca
     4021 ctattcctga tcagagaact ccagttgctt caacacacag tatcagtagt gcagccaccc
     4081 cagatcgaat ccgcttccca agaggcactg ccagtcgtag cactttccac ggccagcccc
     4141 gggaacggcg aaccgcaaca tataatggcc ctcctgcctc tcccagcctg tcccatgaag
     4201 ccacaccatt gtcccagact cgaagccgag gctccactaa tctctttagt aaattaactt
     4261 caaaactcac aaggagtcgc aatgtatctg ctgagcaaaa agatgaaaac aaagaagcaa
     4321 agcctcgatc cctacgcttc acctggagca tgaaaaccac tagttcaatg gatcccgggg
     4381 acatgatgcg ggaaatccgc aaagtgttgg acgccaataa ctgcgactat gagcagaggg
     4441 agcgcttctt gctcttctgc gtccacggag atgggcacgc ggagaacctc gtgcagtggg
     4501 aaatggaagt gtgcaagctg ccaagactgt ctctgaacgg ggtccggttt aagcggatat
     4561 cggggacatc catagccttc aaaaatattg cttccaaaat tgccaatgag ctaaagctgt
     4621 aggacccagc tttcttgtac aaagtggtga tatccagcac agtggcggcc gctcgacaat
     4681 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4741 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4801 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4861 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4921 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4981 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5041 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5101 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5161 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5221 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg
     5281 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5341 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5401 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5461 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5521 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5581 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5641 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5701 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5761 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     5821 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5881 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5941 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6001 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6061 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6121 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6181 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6241 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6301 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6361 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     6421 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
     6481 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6541 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6601 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6661 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6721 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6781 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6841 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6901 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6961 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7081 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7141 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     7201 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7261 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7321 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7381 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7441 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7501 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7561 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7621 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7681 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7741 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9721 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9781 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9841 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9901 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9961 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
    10021 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
    10081 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
    10141 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
    10201 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
    10261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    10441 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    10501 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    10561 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    10621 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    10681 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    10741 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    10801 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    10861 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    10921 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
    10981 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    11041 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    11101 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    11161 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    11221 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    11281 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    11341 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    11401 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    11461 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    11521 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    11581 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    11641 tttgctgtac tttc
//
