LOCUS       sequence-341655-       10739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PSKH1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10739)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PSKH1_IDG-K
FEATURES             Location/Qualifiers
     source          1..10739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     276..295
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             364..963
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(364..383)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     860..880
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1101..1334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1406..1527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1443..1462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1497..1516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1567..1702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1722..1741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1723..1741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1751..1768)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1751..1767)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1760..1782)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1909..2364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1996..2015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2206..2227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2390..2494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2495..2563,2564..3355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3526..4114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4015..4034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4268..4285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4402..4423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4438..4443,4444..4461,4462..4468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4476..4492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4481..4503
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4500..4516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4500..4516
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4535..4555
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4537..4555
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4583..4809
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4810..4990
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5037..5162
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5655..5888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6073..6117
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6384..6500
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6534..6554
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6554..7765
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7602..7621
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7769..7789)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7790..7799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7802..8572
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8579..8620
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8621..8645
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             9771..9779
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(9927..9951)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9981..10569
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10034..10054)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10452..10463)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10583..10739,1..343)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10618..10641)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
       61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      361 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
      421 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
      481 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
      541 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
      601 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
      661 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
      721 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
      781 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
      841 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
      901 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
      961 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct
     1021 cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     1081 tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct
     1141 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     1201 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     1261 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1321 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     1381 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     1441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1501 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     1561 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     1621 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     1681 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     1741 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1801 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1861 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     1921 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1981 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2041 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2101 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     2161 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2221 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2281 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2341 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2401 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2461 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2521 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2581 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2641 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2701 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2761 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2821 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2881 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2941 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3001 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3061 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3121 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3181 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3241 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3301 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3361 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3421 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3481 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3541 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3601 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3661 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3721 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3781 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3841 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3901 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3961 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4021 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4081 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4141 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4201 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4261 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     4321 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     4381 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     4441 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     4501 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     4561 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     4621 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     4681 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     4741 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     4801 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4861 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4921 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4981 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     5041 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     5101 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     5161 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     5221 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     5281 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     5341 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     5401 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     5461 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     5521 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     5581 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     5641 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     5701 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     5761 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     5821 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     5881 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     5941 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     6001 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     6061 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     6121 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     6181 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     6241 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     6301 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     6361 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     6421 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     6481 attacaaaat tcaaaatttt atcgtccagt gtggtggaat tctgcagata tcacaacttt
     6541 gtatagaaaa gttggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc
     6601 acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg
     6661 acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag
     6721 aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc
     6781 ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc
     6841 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag
     6901 ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc
     6961 gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt
     7021 gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg
     7081 gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg
     7141 aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag
     7201 gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc
     7261 atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg
     7321 cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg
     7381 ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct
     7441 gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta
     7501 ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca
     7561 gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac
     7621 tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta
     7681 atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg
     7741 gccgtttttg gcttttttgt tagacggcca acttttgtat acaaagtggg ccaccatggg
     7801 aatcccgctg ctgaacgcta aacagattct gggacagctg gacggcggga gcgtggcagt
     7861 cctgcctgtg gtcgactcca ccaatcagta cctgctggat cgaatcggcg agctgaagag
     7921 tggggatgct tgcattgcag aatatcagca ggcagggaga ggaagcagag ggaggaaatg
     7981 gttctctcct tttggagcta acctgtacct gagtatgttt tggcgcctga agcggggacc
     8041 agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct
     8101 gggagcagac aaggtgcgag tcaaatggcc caatgacctg tatctgcagg atagaaagct
     8161 ggcaggcatc ctggtggagc tggccggaat aacaggcgat gctgcacaga tcgtcattgg
     8221 cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat
     8281 cacactgcag gaagcaggga ttaacctgga caggaatact ctggccgcta tgctgatccg
     8341 agagctgcgg gcagccctgg aactgttcga gcaggaaggc ctggctccat atctgtcacg
     8401 gtgggagaag ctggataact tcatcaatag acccgtgaag ctgatcattg gggacaaaga
     8461 gattttcggg attagccggg ggattgataa acagggagcc ctgctgctgg aacaggacgg
     8521 agttatcaaa ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa aggctagcgg
     8581 caagcccatc cccaaccccc tgctgggcct ggacagcacc acaagtttgt acaaaaaagc
     8641 aggctccacc atgggctgtg ggacaagcaa ggtccttccc gagccaccca aggatgtcca
     8701 gctggatctg gtcaagaagg tggagccctt cagtggcact aagagtgacg tgtacaagca
     8761 cttcatcaca gaggtggaca gtgttggccc tgtcaaagcc gggttcccag cagcaagtca
     8821 gtatgcacac ccctgccccg gtcccccgac tgctggccac acggagcctc cctcagaacc
     8881 accacgcagg gccagggtag ctaagtacag ggccaagttt gacccacgtg ttacagctaa
     8941 gtatgacatc aaggccctaa ttggccgagg cagcttcagc cgagtggtac gtgtagagca
     9001 ccgggcaacc cggcagccgt atgccatcaa gatgattgag accaagtacc gggaggggcg
     9061 ggaggtgtgt gagtcggagc tgcgtgtgct gcgtcgggtg cgtcatgcca acatcatcca
     9121 gctggtggag gtgttcgaga cacaggagcg ggtgtacatg gtgatggagc tggccactgg
     9181 tggagagctc tttgaccgca tcattgccaa gggctccttc accgagcgtg acgccacgcg
     9241 ggtgctgcag atggtgctgg atggcgtccg gtatctgcat gcactgggca tcacacaccg
     9301 agacctcaaa cctgagaatc tgctctacta ccatccgggc actgactcca agatcatcat
     9361 caccgacttc ggcctggcca gtgctcgcaa gaagggtgat gactgcttga tgaagaccac
     9421 ctgtggcacg cctgagtaca ttgccccaga agtcctggtc cgcaagccat acaccaactc
     9481 agtggacatg tgggcgctgg gcgtcattgc ctacatccta ctcagtggca ccatgccgtt
     9541 tgaggatgac aaccgtaccc ggctgtaccg gcagatcctc aggggcaagt acagttactc
     9601 tggggagccc tggcctagtg tgtccaacct ggccaaggac ttcattgacc gcctgctgac
     9661 agtggaccct ggagcccgta tgactgcact gcaggccctg aggcacccgt gggtggtgag
     9721 catggctgcc tcttcatcca tgaagaacct gcaccgctcc atatcccaga acctccttaa
     9781 acgtgcctcc tcgcgctgcc agagcaccaa atctgcccag tccacgcgtt ccagccgctc
     9841 cacacgctcc aataagtcac gccgtgtgcg ggaacgggag ctgcgggagc tcaacctgcg
     9901 ctaccagcag caatacaatg gctaggaccc agctttcttg tacaaagtgg tgatatccag
     9961 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
    10021 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    10081 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    10141 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    10201 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    10261 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    10321 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
    10381 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    10441 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    10501 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    10561 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    10621 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    10681 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcc
//
