LOCUS       sequence-341656-       11123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RIOK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11123)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-RIOK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..11123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     480..500
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             721..954
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1026..1147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1063..1082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1117..1136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1187..1322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1342..1361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1343..1361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1371..1388)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1371..1387)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1380..1402)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1529..1984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1616..1635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2010..2114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2115..2183,2184..2975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3146..3734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3635..3654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3888..3905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4022..4043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4058..4063,4064..4081,4082..4088)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4096..4112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4101..4123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4120..4136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4120..4136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4155..4175
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4157..4175
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4203..4429
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4430..4610
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4657..4782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5275..5508
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5693..5737
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6004..6120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6154..6174
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6174..7385
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7222..7241
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7389..7409)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7410..7419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7422..8192
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8199..8240
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8241..8265
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9931..9955)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9985..10573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10038..10058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10456..10467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10587..11086
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10622..10645)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11019..11038
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(11107..11123,1..583)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(11107..11123,1..3))
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
       61 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
      121 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
      181 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
      241 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
      301 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
      361 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
      421 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
      481 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
      541 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
      601 gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa
      661 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
      721 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      781 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      841 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      901 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
      961 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     1021 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1141 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     1201 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     1261 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     1321 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     1381 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1441 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1501 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     1561 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1621 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1681 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1741 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1801 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1861 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     1921 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     1981 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2041 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2101 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2161 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2221 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2281 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2341 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2401 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2461 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2521 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2581 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2641 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2701 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2761 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2821 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2881 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2941 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3001 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3061 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3601 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3661 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3721 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3781 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3841 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3901 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3961 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4021 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4081 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4141 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     4201 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     4261 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     4321 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     4381 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     4441 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4501 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4561 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     4621 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     4681 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     4741 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     4801 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     4861 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     4921 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     4981 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     5041 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     5101 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     5161 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     5221 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     5281 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     5341 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     5401 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     5461 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     5521 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     5581 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     5641 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     5701 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     5761 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     5821 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     5881 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     5941 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     6001 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     6061 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     6121 atcgtccagt gtggtggaat tctgcagata tcacaacttt gtatagaaaa gttggcctcc
     6181 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     6241 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     6301 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     6361 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     6421 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     6481 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     6541 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
     6601 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     6661 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
     6721 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
     6781 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
     6841 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     6901 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     6961 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     7021 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
     7081 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     7141 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     7201 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     7261 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     7321 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
     7381 tagacggcca acttttgtat acaaagtggg ccaccatggg aatcccgctg ctgaacgcta
     7441 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     7501 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     7561 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     7621 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     7681 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     7741 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     7801 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     7861 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     7921 ttaacctgga caggaatact ctggccgcta tgctgatccg agagctgcgg gcagccctgg
     7981 aactgttcga gcaggaaggc ctggctccat atctgtcacg gtgggagaag ctggataact
     8041 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     8101 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     8161 gcggagaaat cagtctgcgg tctgccgaaa aggctagcgg caagcccatc cccaaccccc
     8221 tgctgggcct ggacagcacc acaagtttgt acaaaaaagc aggctccacc atggggaaag
     8281 tgaatgtggc caagttgcgt tacatgagcc gagatgactt cagggtcttg accgcggttg
     8341 aaatgggcat gaagaaccat gaaattgttc ccggcagttt gattgcttct atagccagcc
     8401 ttaaacatgg tggctgtaat aaagttttaa gagaattagt gaaacataaa ctcatagctt
     8461 gggagcgtac caaaactgtc cagggctatc ggttgacaaa tgcaggatat gattacctag
     8521 ctttgaaaac actttcttct aggcaagtag ttgagtctgt tggaaaccag atgggtgttg
     8581 gcaaagaatc agatatttac attgttgcaa atgaagaagg acaacaattt gcattaaagc
     8641 ttcacagact aggaagaacc tcgtttcgaa atttgaaaaa caaacgcgat tatcataaat
     8701 ataggcacaa tgtgtcatgg ctatatttat ctcgtctctc tgccatgaag gaatttgcct
     8761 atatgaaggc attgtatgag aggaaatttc cagttccaaa gccaattgat tacaatcgtc
     8821 atgcagtggt catggaactc ataaatggtt atccactatg tcagatacac catgttgaag
     8881 atcctgcatc agtatatgat gaagctatgg aactaattgt caaacttgca aatcatgggc
     8941 tgattcatgg agattttaat gaatttaatc tcattttgga tgaaagtgac catatcacca
     9001 tgattgattt tccacagatg gtttcaactt ctcatcccaa tgctgagtgg tattttgaca
     9061 gagatgttaa atgcattaaa gatttcttta tgaaacgttt cagctacgaa agtgagcttt
     9121 ttccaacttt taaggatatc aggagagaag acactcttga tgtggaggtt tctgccagtg
     9181 gctacacaaa ggaaatgcag gcagatgatg aactgcttca tccattaggt ccagatgata
     9241 aaaatattga aacaaaagag ggatctgaat tctcattttc agatggagaa gtggcagaaa
     9301 aagcagaggt ttacgggtca gaaaatgaaa gtgaacggaa ctgtctagaa gaatcagagg
     9361 gctgctattg cagatcatct ggagaccctg aacaaataaa ggaagacagt ttatcagaag
     9421 agagtgctga tgcacggagt tttgaaatga ctgaattcaa tcaagcttta gaagaaataa
     9481 aagggcaggt tgttgaaaac aactctgtaa ctgaattttc tgaggagaaa aacagaactg
     9541 aaaattacaa caggcaagat ggtcagagag ttcaaggagg agtccctgct ggctctgacg
     9601 agtatgaaga tgaatgccct catctaattg ccttgtcgtc attaaataga gaattcaggc
     9661 ctttcagaga tgaagaaaat gtgggagcta tgaatcagta tagaacaaga actctgagta
     9721 tcacttcttc aggcagtgct gtaagctgtt caacaattcc tccagaactg gtgaaacaga
     9781 aggtgaaacg tcagttgaca aaacagcaaa aatcagctgt cagacgtcga ttgcagaaag
     9841 gagaagcaaa tatatttacc aagcaacgta gggaaaacat gcaaaatatc aaatcaagtt
     9901 tggaagcagc cagcttttgg ggagaatagg acccagcttt cttgtacaaa gtggtgatat
     9961 ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
    10021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    10081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    10141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    10201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    10261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    10321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
    10381 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    10441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    10501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    10561 gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
    10621 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
    10681 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
    10741 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
    10801 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
    10861 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
    10921 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
    10981 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
    11041 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
    11101 cttaccatga ccgagtacaa gcc
//
