LOCUS       sequence-341657-       11957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TBCK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11957)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TBCK_IDG-K
FEATURES             Location/Qualifiers
     source          1..11957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..69
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             138..737
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(138..157)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     634..654
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             875..1108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1180..1301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1217..1236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1271..1290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1341..1476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1496..1515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1497..1515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1525..1542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1525..1541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1534..1556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1683..2138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1770..1789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1980..2001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2164..2268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2269..2337,2338..3129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3300..3888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3789..3808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4042..4059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4176..4197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4212..4217,4218..4235,4236..4242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4250..4266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4255..4277
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4274..4290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4274..4290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4309..4329
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4311..4329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4357..4583
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4584..4764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4811..4936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5429..5662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5847..5891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6158..6274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6308..6328
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6328..7539
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7376..7395
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7543..7563)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7564..7573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7576..8346
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8353..8394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8395..8419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10919..10943)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10973..11561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11026..11046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11444..11455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(11575..11957,1..117)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11610..11633)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
       61 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      121 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
      181 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
      241 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
      301 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
      361 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
      421 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
      481 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
      541 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
      601 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
      661 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
      721 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
      781 gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
      841 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
      901 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
      961 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1021 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1081 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     1141 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     1201 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1261 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     1321 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     1381 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     1441 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     1501 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1681 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1741 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1861 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1981 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     2041 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2101 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     2161 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3421 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     4201 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4261 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4321 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     4381 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     4441 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     4501 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     4561 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     4621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4741 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     4801 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4861 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4921 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4981 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     5041 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     5101 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     5161 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     5221 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     5281 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     5341 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     5401 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     5461 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     5521 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     5581 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     5641 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     5701 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     5761 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     5821 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     5881 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     5941 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     6001 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     6061 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     6121 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     6181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     6241 aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca
     6301 gatatcacaa ctttgtatag aaaagttggc ctccgcgccg ggttttggcg cctcccgcgg
     6361 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
     6421 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
     6481 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
     6541 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
     6601 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
     6661 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
     6721 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
     6781 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
     6841 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
     6901 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
     6961 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     7021 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     7081 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     7141 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     7201 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     7261 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     7321 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     7381 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     7441 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     7501 tccgctaaat tctggccgtt tttggctttt ttgttagacg gccaactttt gtatacaaag
     7561 tgggccacca tgggaatccc gctgctgaac gctaaacaga ttctgggaca gctggacggc
     7621 gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct ggatcgaatc
     7681 ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg gagaggaagc
     7741 agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat gttttggcgc
     7801 ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt catggcagaa
     7861 gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga cctgtatctg
     7921 caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg cgatgctgca
     7981 cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga aagcgtggtc
     8041 aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa tactctggcc
     8101 gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga aggcctggct
     8161 ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt gaagctgatc
     8221 attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg agccctgctg
     8281 ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct gcggtctgcc
     8341 gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag caccacaagt
     8401 ttgtacaaaa aagcaggctc caccatgttt cccctgaagg acgctgaaat gggagccttt
     8461 accttctttg cctcggctct gccacatgat gtttgtggaa gcaatggact tcctctcaca
     8521 ccaaattcca tcaaaatttt agggcgcttt caaatcctta aaaccatcac ccatcccaga
     8581 ctctgccagt atgtggatat ttctagggga aagcatgaac gactagtggt cgtggctgaa
     8641 cattgtgaac gtagtctgga agacttgctt cgagaaagga aacctgtgag gtatccctcg
     8701 tacttggccc ctgaggtaat tgcacaggga attttcaaaa ccactgatca catgccaagt
     8761 aaaaaaccat tgccttctgg ccccaaatca gatgtatggt ctcttggaat cattttattt
     8821 gagctttgtg tgggaagaaa attatttcag agcttggata tttctgaaag actaaaattt
     8881 ttgcttactt tggattgtgt agatgacact ttaatagttc tggctgaaga gcatggttgt
     8941 ttggacatta taaaggagct tcctgaaact gtgatagatc ttttgaataa gtgccttacc
     9001 ttccatcctt ctaagaggcc aaccccagat gaattaatga aggacaaagt attcagtgag
     9061 gtatcacctt tatatacccc ctttaccaaa cctgccagtc tgttttcatc ttctctgaga
     9121 tgtgctgatt taactctgcc tgaggatatc agtcagttgt gtaaagatat aaataatgat
     9181 tacctggcag aaagatctat tgaagaagtg tattaccttt ggtgtttggc tggaggtgac
     9241 ttggagaaag agcttgtcaa caaggaaatc attcgatcca aaccacctat ctgcacactc
     9301 cccaattttc tctttgagga tggtgaaagc tttggacaag gtcgagatag aagctcgctt
     9361 ttagatgata ccactgtgac attgtcgtta tgccagctaa gaaatagatt gaaagatgtt
     9421 ggtggagaag cattttaccc attacttgaa gatgaccagt ctaatttacc tcattcaaac
     9481 agcaataatg agttgtctgc agctgccacg ctccctttaa tcatcagaga gaaggataca
     9541 gagtaccaac taaatagaat tattctcttc gacaggctgc taaaggctta tccatataaa
     9601 aaaaaccaaa tctggaaaga agcaagagtt gacattcctc ctcttatgag aggtttaacc
     9661 tgggctgctc ttctgggagt tgagggagct attcatgcca agtacgatgc aattgataaa
     9721 gacactccaa ttcctacaga tagacaaatt gaagtggata ttcctcgctg tcatcagtac
     9781 gatgaactgt tatcatcacc agaaggtcat gcaaaattta ggcgtgtatt aaaagcctgg
     9841 gtagtgtctc atcctgatct tgtgtattgg caaggtcttg actcactttg tgctccattc
     9901 ctatatctaa acttcaataa tgaagccttg gcttatgcat gtatgtctgc ttttattccc
     9961 aaatacctgt ataacttctt cttaaaagac aactcacatg taatacaaga gtatctgact
    10021 gtcttctctc agatgattgc atttcatgat ccagagctga gtaatcatct caatgagatt
    10081 ggtttcattc cagatctcta tgccatccct tggtttctta ccatgtttac tcatgtattt
    10141 ccactacaca aaattttcca cctctgggat accttactac ttgggaattc ctctttccca
    10201 ttctgtattg gagtagcaat tcttcagcag ctgcgggacc ggcttttggc taatggcttt
    10261 aatgagtgta ttcttctctt ctccgattta ccagaaattg acattgaacg ctgtgtgaga
    10321 gaatctatca acctgttttg ttggactcct aaaagtgcta cttacagaca gcatgctcaa
    10381 cctccaaagc catcttctga cagcagtgga ggcagaagtt cggcacctta tttctctgct
    10441 gagtgtccag atcctccaaa gacagatctg tcaagagaat ccatcccatt aaatgacctg
    10501 aagtcagaag tatcaccacg gatttcagca gaggacctga ttgacttgtg tgagctcaca
    10561 gtgacaggcc acttcaaaac acccagcaag aaaacaaagt ccagtaaacc aaagctcctg
    10621 gtggttgaca tccggaatag tgaagacttt attcgtggtc acatttcagg aagcatcaac
    10681 attccattca gtgctgcctt cactgcagaa ggggagctta cccagggccc ttacactgct
    10741 atgctccaga acttcaaagg gaaggtcatt gtcatcgtgg ggcatgtggc aaaacacaca
    10801 gctgagtttg cagctcacct tgtgaagatg aaatatccaa gaatctgtat tctagatggt
    10861 ggcattaata aaataaagcc aacaggcctc ctcaccatcc catctcctca aatataggac
    10921 ccagctttct tgtacaaagt ggtgatatcc agcacagtgg cggccgctcg acaatcaacc
    10981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    11041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    11101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    11161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    11221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    11281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    11341 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
    11401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    11461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    11521 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag
    11581 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
    11641 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
    11701 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
    11761 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
    11821 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
    11881 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
    11941 ccgggggcgg gctcagg
//
