LOCUS       sequence-341658-       11765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TLK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11765)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TLK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     423..443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             664..897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    969..1090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1006..1025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1060..1079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1130..1265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1285..1304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1286..1304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1314..1331)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1314..1330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1323..1345)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1472..1927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1559..1578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1769..1790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1953..2057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2058..2126,2127..2918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3089..3677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3578..3597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3831..3848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3965..3986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4001..4006,4007..4024,4025..4031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4039..4055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4044..4066
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4063..4079
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4063..4079
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4098..4118
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4100..4118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4146..4372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4373..4553
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4600..4725
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5218..5451
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5636..5680
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5947..6063
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6097..6117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6117..7328
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7165..7184
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7332..7352)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7353..7362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7365..8135
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8142..8183
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8184..8208
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10516..10540)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10570..11158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10623..10643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11041..11052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11172..11671
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11207..11230)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11604..11623
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(11692..11765,1..526)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11692..11711)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
       61 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
      121 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
      181 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
      241 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
      301 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
      361 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
      421 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
      481 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
      541 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
      601 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
      661 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
      721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
      901 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
      961 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1081 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     1141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     1261 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     1321 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1381 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1441 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     1501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     1921 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2281 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2581 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3961 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4021 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4081 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     4141 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     4201 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     4261 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     4321 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     4381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     4561 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     4621 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     4681 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4741 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4801 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4861 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4921 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4981 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     5041 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     5101 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     5161 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5221 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     5281 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     5341 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     5401 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     5461 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5521 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5581 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5641 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5701 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5761 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5821 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5881 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     5941 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     6001 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     6061 tttatcgtcc agtgtggtgg aattctgcag atatcacaac tttgtataga aaagttggcc
     6121 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     6181 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     6241 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     6301 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     6361 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     6421 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     6481 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     6541 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     6601 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     6661 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     6721 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     6781 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     6841 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     6901 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     6961 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     7021 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     7081 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     7141 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     7201 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     7261 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     7321 tgttagacgg ccaacttttg tatacaaagt gggccaccat gggaatcccg ctgctgaacg
     7381 ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct gtggtcgact
     7441 ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat gcttgcattg
     7501 cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct ccttttggag
     7561 ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca atcggcctgg
     7621 gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca gacaaggtgc
     7681 gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc atcctggtgg
     7741 agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg attaacgtgg
     7801 ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg caggaagcag
     7861 ggattaacct ggacaggaat actctggccg ctatgctgat ccgagagctg cgggcagccc
     7921 tggaactgtt cgagcaggaa ggcctggctc catatctgtc acggtgggag aagctggata
     7981 acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc gggattagcc
     8041 gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc aaaccctgga
     8101 tgggcggaga aatcagtctg cggtctgccg aaaaggctag cggcaagccc atccccaacc
     8161 ccctgctggg cctggacagc accacaagtt tgtacaaaaa agcaggctcc accatgagtg
     8221 tccaaagtag cagtggaagt ttggaggggc cgccatcttg gtcccagctc tccacgtctc
     8281 caaccccggg ctcggcggcg gcggccaggt ccctgctgaa tcacacgccg ccatccggga
     8341 ggcccaggga aggtgcaatg gatgagcttc atagtctgga tccaagaagg caagagttat
     8401 tggaagctag atttactgga gttgcaagtg ggagcactgg aagtacgggc agttgcagtg
     8461 ttggagctaa agcctcaaca aataacgaaa gctctaatca cagttttgga agcttgggat
     8521 ctttaagtga caaagaatca gagacaccgg agaagaaaca atcggaatca tccaggggaa
     8581 gaaagagaaa agcagaaaac cagaatgaaa gtagtcaggg aaaaagtatt gggggacgtg
     8641 gccacaaaat tagcgactat tttgaatacc agggtggaaa tggctcaagt ccagtaagag
     8701 gcatacctcc tgcaatccgt tctcctcaaa attcacattc acattccact ccttcctcat
     8761 ctgttcgacc gaatagccct tctcctactg cattagcatt tggggaccac cctattgtac
     8821 aaccaaagca attatccttt aaaattattc agactgatct cacaatgctg aaattagcag
     8881 cattagaaag taataaaatc caggacctgg aaaagaagga gggacgtata gatgatttgc
     8941 tcagggctaa ctgtgatctc agacggcaaa tagatgaaca acaaaaatta cttgaaaaat
     9001 acaaagaacg attaaataag tgcatatcaa tgagcaagaa acttcttatt gaaaagagta
     9061 cacaagaaaa gctgtcaagc agagagaaga gtatgcaaga tcgattacgc cttgggcact
     9121 ttacaacagt tagacatggc gcttcattta ctgaacaatg gacagatggt tttgcatttc
     9181 agaatcttgt gaagcaacaa gaatgggtga atcagcaaag ggaagatatt gaaaggcaaa
     9241 ggaaacttct agccaaacgc aaacctccca cagctaataa ttctcaggca ccctctacca
     9301 attctgaacc aaaacaaagg aaaaacaaag cagtcaatgg agcagagaat gatccctttg
     9361 ttagaccaaa tttaccacaa ctgttgactt tggcagaata tcatgaacag gaagaaattt
     9421 tcaaacttag actaggacat ctcaaaaagg aagaggcaga aatccaggca gaacttgaac
     9481 gtttggaaag agtcagaaat cttcacatac gtgagctgaa aagaataaac aatgaagata
     9541 attcacagtt caaagatcac ccaacattaa atgaaagata tttattactt catctgcttg
     9601 gtagaggtgg ctttagtgaa gtgtataagg cttttgacct ttatgaacaa agatatgctg
     9661 ctgtgaagat acatcagctt aataaaagct ggagagatga gaagaaagaa aactaccaca
     9721 aacatgcctg cagagagtat agaatacaca aagaactgga tcaccccaga atagttaaac
     9781 tctatgatta tttctccttg gatacagata cgttttgtac agtgttagaa tactgtgaag
     9841 gcaatgactt ggatttctat ctgaagcaac acaagttaat gtcagagaaa gaagctcggt
     9901 ctattgtaat gcagattgta aatgcactaa gatatctcaa tgagatcaaa ccccctatta
     9961 tacattatga tcttaagcca ggaaacatcc tactggtaga tggaacagca tgtggtgaaa
    10021 tcaaaatcac tgattttggt ctgtccaaga ttatggatga tgatagctat ggtgtagatg
    10081 gaatggatct aacttcccag ggggcaggca cttactggta tttacctcct gagtgttttg
    10141 tagttggaaa agagccacca aagatttcca acaaggttga tgtatggtcg gttggagtca
    10201 tcttctttca gtgtctttat ggtagaaagc catttggtca caatcaatct caacaagaca
    10261 ttcttcaaga aaatacaata ttaaaagcca cagaagtcca gttccctgta aaaccggttg
    10321 taagcagtga agccaaggca tttataagac gctgtttggc atatcgaaaa gaagatcgat
    10381 ttgatgtgca ccagctggca aatgacccat accttctccc acacatgaga agatcaaatt
    10441 cttcaggaaa cctacacatg gctgggctga cagcatcccc tacaccccct tcttcaagca
    10501 taattactta ctaggaccca gctttcttgt acaaagtggt gatatccagc acagtggcgg
    10561 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10621 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10681 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10741 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10801 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10861 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10921 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
    10981 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    11041 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    11101 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
    11161 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    11221 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    11281 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    11341 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    11401 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    11461 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    11521 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    11581 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    11641 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
    11701 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    11761 gccgc
//
