LOCUS       sequence-341659-       11567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PRKCQ (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11567)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PRKCQ-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             136..735
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(136..155)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     632..652
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             873..1106
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1178..1299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1215..1234)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1269..1288
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1339..1474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1494..1513)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1495..1513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1523..1540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1523..1539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1532..1554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1681..2136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1768..1787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1978..1999
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2162..2266
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2267..2335,2336..3127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3298..3886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3787..3806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4040..4057
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4174..4195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4210..4215,4216..4233,4234..4240)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4248..4264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4253..4275
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4272..4288
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4272..4288
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4307..4327
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4309..4327
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4355..4581
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4582..4762
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4809..4934
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5427..5660
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5845..5889
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6156..6272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6304..6324
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6324..7535
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7372..7391
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    7536..7560
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9682..9706)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9708..9749
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9756..10526
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(10532..10552)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    10581..11169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10634..10654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11052..11063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(11183..11567,1..115)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11218..11241)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
       61 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
      121 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
      181 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
      241 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
      301 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
      361 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
      421 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
      481 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
      541 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
      601 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
      661 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
      721 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
      781 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
      841 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
      901 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
      961 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1021 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1081 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     1141 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     1201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1261 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     1321 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1381 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1441 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     1501 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1561 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1621 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     1681 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1741 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1861 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1921 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1981 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2041 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2101 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     2161 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2221 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2281 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2341 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2401 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2461 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2521 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2581 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2641 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2701 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2761 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2821 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2881 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2941 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3001 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3061 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3121 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3181 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3241 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3301 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3361 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3421 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3481 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3541 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3601 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3661 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3721 aaaggtggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3781 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3841 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3901 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3961 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4021 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4201 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4261 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     4321 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     4381 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     4441 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     4501 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     4561 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     4621 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4681 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4741 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     4801 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     4861 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     4921 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     4981 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     5041 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     5101 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     5161 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     5221 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     5281 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     5341 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     5401 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     5461 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     5521 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     5581 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     5641 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     5701 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     5761 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     5821 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     5881 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5941 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6001 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6061 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6121 cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
     6181 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     6241 ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca gtgtggtgga attctgcaga
     6301 tatcaacttt gtatagaaaa gttggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     6361 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     6421 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     6481 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     6541 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     6601 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     6661 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     6721 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     6781 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     6841 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     6901 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     6961 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     7021 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     7081 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     7141 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     7201 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     7261 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     7321 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     7381 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     7441 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     7501 ctaaattctg gccgtttttg gcttttttgt tagacacaag tttgtacaaa aaagttggca
     7561 ccatgtcgcc atttcttcgg attggcttgt ccaactttga ctgcgggtcc tgccagtctt
     7621 gtcagggcga ggctgttaac ccttactgtg ctgtgctcgt caaagagtat gtcgaatcag
     7681 agaacgggca gatgtatatc cagaaaaagc ctaccatgta cccaccctgg gacagcactt
     7741 ttgatgccca tatcaacaag ggaagagtca tgcagatcat tgtgaaaggc aaaaacgtgg
     7801 acctcatctc tgaaaccacc gtggagctct actcgctggc tgagaggtgc aggaagaaca
     7861 acgggaagac agaaatatgg ttagagctga aacctcaagg ccgaatgcta atgaatgcaa
     7921 gatactttct ggaaatgagt gacacaaagg acatgaatga atttgagacg gaaggcttct
     7981 ttgctttgca tcagcgccgg ggtgccatca agcaggcaaa ggtccaccac gtcaagtgcc
     8041 acgagttcac tgccaccttc ttcccacagc ccacattttg ctctgtctgc cacgagtttg
     8101 tctggggcct gaacaaacag ggctaccagt gccgacaatg caatgcagca attcacaaga
     8161 agtgtattga taaagttata gcaaagtgca caggatcagc tatcaatagc cgagaaacca
     8221 tgttccacaa ggagagattc aaaattgaca tgccacacag atttaaagtc tacaattaca
     8281 agagcccgac cttctgtgaa cactgtggga ccctgctgtg gggactggca cggcaaggac
     8341 tcaagtgtga tgcatgtggc atgaatgtgc atcatagatg ccagacaaag gtggccaacc
     8401 tttgtggcat aaaccagaag ctaatggctg aagcgctggc catgattgag agcactcaac
     8461 aggctcgctg cttaagagat actgaacaga tcttcagaga aggtccggtt gaaattggtc
     8521 tcccatgctc catcaaaaat gaagcaaggc cgccatgttt accgacaccg ggaaaaagag
     8581 agcctcaggg catttcctgg gagtctccgt tggatgaggt ggataaaatg tgccatcttc
     8641 cagaacctga actgaacaaa gaaagaccat ctctgcagat taaactaaaa attgaggatt
     8701 ttatcttgca caaaatgttg gggaaaggaa gttttggcaa ggtcttcctg gcagaattca
     8761 agaaaaccaa tcaatttttc gcaataaagg ccttaaagaa agatgtggtc ttgatggacg
     8821 atgatgttga gtgcacgatg gtagagaaga gagttctttc cttggcctgg gagcatccgt
     8881 ttctgacgca catgttttgt acattccaga ccaaggaaaa cctctttttt gtgatggagt
     8941 acctcaacgg aggggactta atgtaccaca tccaaagctg ccacaagttc gacctttcca
     9001 gagcgacgtt ttatgctgct gaaatcattc ttggtctgca gttccttcat tccaaaggaa
     9061 tagtctacag ggacctgaag ctagataaca tcctgttaga caaagatgga catatcaaga
     9121 tcgcggattt tggaatgtgc aaggagaaca tgttaggaga tgccaagacg aataccttct
     9181 gtgggacacc tgactacatc gccccagaga tcttgctggg tcagaaatac aaccactctg
     9241 tggactggtg gtccttcggg gttctccttt atgaaatgct gattggtcag tcgcctttcc
     9301 acgggcagga tgaggaggag ctcttccact ccatccgcat ggacaatccc ttttacccac
     9361 ggtggctgga gaaggaagca aaggaccttc tggtgaagct cttcgtgcga gaacctgaga
     9421 agaggctggg cgtgagggga gacatccgcc agcacccttt gtttcgggag atcaactggg
     9481 aggaacttga acggaaggag attgacccac cgttccggcc gaaagtgaaa tcaccatttg
     9541 actgcagcaa tttcgacaaa gaattcttaa acgagaagcc ccggctgtca tttgccgaca
     9601 gagcactgat caacagcatg gaccagaata tgttcaggaa cttttccttc atgaaccccg
     9661 ggatggagcg gctgatatcc ttgccaactt tcttgtacaa agtgggcggc aagcccatcc
     9721 ccaaccccct gctgggcctg gacagcaccg ctagcatccc gctgctgaac gctaaacaga
     9781 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     9841 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     9901 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     9961 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
    10021 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
    10081 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
    10141 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
    10201 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
    10261 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
    10321 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
    10381 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
    10441 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
    10501 aaatcagtct gcggtctgcc gaaaagtaag ccaactttat tatacatagt tgatatccag
    10561 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
    10621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    10681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    10741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    10801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    10861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    10921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
    10981 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    11041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    11101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    11161 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    11221 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    11281 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
    11341 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
    11401 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
    11461 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
    11521 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcagggg
//
