LOCUS       sequence-341661-       10310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RSKR (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10310)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-RSKR_IDG-K
FEATURES             Location/Qualifiers
     source          1..10310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(69..88)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     565..585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             806..1039
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1111..1232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1148..1167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1202..1221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1272..1407
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1427..1446)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1428..1446)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1456..1473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1456..1472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1465..1487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1614..2069
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1701..1720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1911..1932
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2095..2199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2200..2268,2269..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3231..3819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3720..3739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3973..3990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4107..4128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4143..4148,4149..4166,4167..4173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4181..4197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4186..4208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4205..4221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4205..4221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4240..4260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4242..4260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4288..4514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4515..4695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4742..4867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5360..5593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5778..5822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6089..6205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6239..6259
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6259..7470
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7307..7326
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7474..7494)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7495..7504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7507..8277
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8284..8325
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8326..8350
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9203..9227)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9257..9845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9310..9330)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9728..9739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(9859..10310,1..48)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9894..9917)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10291..10310
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
       61 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
      121 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
      181 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
      241 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
      301 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
      361 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
      421 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
      481 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
      541 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
      601 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
      661 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
      721 catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
      781 cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat
      841 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      901 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      961 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1021 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     1081 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     1141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     1261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     1321 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     1381 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     1441 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1501 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1561 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     1621 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1681 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1741 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1801 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     1861 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1921 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     1981 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2041 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     2101 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2161 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2221 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2281 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2341 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2401 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2461 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2521 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2581 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2641 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2701 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2761 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2821 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2881 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2941 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3001 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3061 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3361 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3901 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4021 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4081 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4141 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4201 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     4261 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     4321 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     4381 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     4441 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     4501 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4681 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     4741 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     4801 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     4861 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     4921 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     4981 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     5041 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     5101 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     5161 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     5221 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     5281 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     5341 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     5401 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     5461 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     5521 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     5581 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     5641 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     5701 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     5761 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     5821 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     5881 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     5941 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     6001 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     6061 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     6121 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     6181 aacaaattac aaaattcaaa attttatcgt ccagtgtggt ggaattctgc agatatcaca
     6241 actttgtata gaaaagttgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     6301 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     6361 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     6421 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     6481 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     6541 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     6601 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     6661 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     6721 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     6781 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     6841 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     6901 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     6961 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     7021 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     7081 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     7141 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     7201 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     7261 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     7321 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     7381 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     7441 ttctggccgt ttttggcttt tttgttagac ggccaacttt tgtatacaaa gtgggccacc
     7501 atgggaatcc cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg
     7561 gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg
     7621 aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg
     7681 aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg
     7741 ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga
     7801 aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga
     7861 aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc
     7921 attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc
     7981 tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg
     8041 atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg
     8101 tcacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac
     8161 aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag
     8221 gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggct
     8281 agcggcaagc ccatccccaa ccccctgctg ggcctggaca gcaccacaag tttgtacaaa
     8341 aaagcagaag ggccgtcaag gcccaccatg ggagcagtaa gctgtcggca ggggcagcac
     8401 acccagcagg gggaacacac ccgggtggct gtccctcaca agcagggtgg caacatccgg
     8461 ggtccctggg cccgaggctg gaagagcctc tggacaggtt tgggaaccat caggtcagat
     8521 ctggaagaac tctgggaact acgggggcac cactatctgc accaggaatc cctaaagcca
     8581 gccccagtac tggtagagaa gcctctgcca gagtggccag tgcctcagtt catcaacctc
     8641 tttctaccag agtttcccat taggcccatt agggggcagc agcagctgaa gattttaggc
     8701 ctcgtggcta aaggctcctt tggaactgtc ctcaaggtgc tagattgcac ccagaaagct
     8761 gtatttgcag tgaaggtggt gcccaaggta aaggtcctac agagggatac cgtgaggcag
     8821 tgcaaagagg aggttagcat ccagcgacag atcaaccatc cctttgtaca cagcttgggg
     8881 gacagctggc agggaaaacg gcaccttttc attatgtgta gctactgcag cacagatctg
     8941 tactcccttt ggtcggctgt tggctgcttt cctgaggctt ccatccgtct ctttgctgcc
     9001 gagttggtgc tggtactgtg ttatctccat gacttgggca tcatgcatcg agatgtgaag
     9061 atggagaata ttcttctaga tgaacgaggc catctgaaac tgacagactt tggtctgtcc
     9121 cgccacgtgc cccagggagc tcaagcctac actatctgtg gcactcttca gtacatgggt
     9181 gagagaggtt agggcctcat gggcccagct ttcttgtaca aagtggtgat atccagcaca
     9241 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     9301 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     9361 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     9421 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     9481 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     9541 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     9601 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     9661 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     9721 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     9781 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     9841 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     9901 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     9961 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
    10021 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
    10081 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
    10141 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
    10201 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
    10261 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
//
