LOCUS       sequence-341662-       10298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of MAPK15 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10298)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-MAPK15_IDG-K
FEATURES             Location/Qualifiers
     source          1..10298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             125..724
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(125..144)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     621..641
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             862..1095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1167..1288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1204..1223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1258..1277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1328..1463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1483..1502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1484..1502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1512..1529)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1512..1528)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1521..1543)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1670..2125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1757..1776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1967..1988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2151..2255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2256..2324,2325..3116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3287..3875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3776..3795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4029..4046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4163..4184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4199..4204,4205..4222,4223..4229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4237..4253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4242..4264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4261..4277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4261..4277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4296..4316
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4298..4316
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4344..4570
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4571..4751
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4798..4923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5416..5649
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5834..5878
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6145..6261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6295..6315
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6315..7526
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7363..7382
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7530..7550)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7551..7560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7563..8333
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8340..8381
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8382..8406
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9247..9271)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9301..9889
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9354..9374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9772..9783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(9903..10298,1..104)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9938..9961)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
       61 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
      121 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
      181 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
      241 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
      301 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
      361 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
      421 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
      481 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
      541 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
      601 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
      661 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
      721 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
      781 tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
      841 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
      901 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      961 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     1021 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     1081 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     1141 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     1201 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1261 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     1321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     1381 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     1441 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     1501 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1561 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1621 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     1681 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1741 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1801 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1861 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     1921 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1981 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2041 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     2101 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4081 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4141 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4201 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4261 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     4321 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     4381 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     4441 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     4501 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     4561 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4741 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     4801 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     4861 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     4921 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     4981 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     5041 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     5101 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     5161 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     5221 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     5281 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     5341 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     5401 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     5461 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     5521 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     5581 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     5641 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     5701 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     5761 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     5821 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     5881 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     5941 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     6001 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     6061 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     6121 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     6181 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     6241 aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcacaactt
     6301 tgtatagaaa agttggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     6361 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
     6421 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
     6481 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
     6541 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
     6601 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
     6661 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
     6721 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
     6781 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
     6841 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
     6901 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
     6961 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
     7021 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     7081 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     7141 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     7201 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     7261 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     7321 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     7381 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     7441 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     7501 ggccgttttt ggcttttttg ttagacggcc aacttttgta tacaaagtgg gccaccatgg
     7561 gaatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag
     7621 tcctgcctgt ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga
     7681 gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat
     7741 ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac
     7801 cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc
     7861 tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc
     7921 tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg
     7981 gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga
     8041 tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct atgctgatcc
     8101 gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgtcac
     8161 ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag
     8221 agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg gaacaggacg
     8281 gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa aaggctagcg
     8341 gcaagcccat ccccaacccc ctgctgggcc tggacagcac cacaagtttg tacaaaaaag
     8401 caggctccac catgtgcacc gtagtggacc ctcgcattgt ccggagatac ctactcaggc
     8461 ggcagctcgg gcagggggcc tatggcattg tgtggaaggc agtggaccgg aggactggtg
     8521 aggtcgtggc catcaagaaa atctttgatg cttttaggga taagacagat gcccagagaa
     8581 cattccggga aatcacgctc ctccaggagt ttggggacca tcccaacatc atcagcctcc
     8641 ttgacgtgat ccgggcagag aacgacaggg acatttacct ggtgtttgag tttatgggtt
     8701 gcccccccag ccccccaccc ccgactgcag tgcgcaccct ctctgcagac actgacctga
     8761 acgcagtcat ccggaagggc ggcctgctgc aggacgtcca cgtgcgctcc atcttctacc
     8821 agctcctgcg ggccacccgg ttcctccact cggggcacgt tgtgcaccgg gaccagaagc
     8881 cgtccaatgt gctcctggat gccaactgca cagtgaagct gtgtgacttt ggcctggccc
     8941 gctccctggg cgacctccct gaggggcctg aggaccaggc cgtgacagag tacgtggcca
     9001 cacgctggta ccgagcaccg gaggtgctgc tctcttcgca ccgatacacc cttggggtgg
     9061 acatgtggag tctgggctgt atcctggggg agatgctgcg ggggagaccc ctgttccccg
     9121 gcacgtccac cctccaccag ctggagctga tcctggagac catcccaccg ccatctgagg
     9181 aggacctcct ggctctcggc tcaggctgcc gtgcctctgt gctgcaccag ctggggtccc
     9241 ggtaggaccc agctttcttg tacaaagtgg tgatatccag cacagtggcg gccgctcgac
     9301 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9361 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9421 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9481 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9541 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9601 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9661 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     9721 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9781 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9841 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
     9901 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     9961 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
    10021 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
    10081 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
    10141 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
    10201 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
    10261 gggtgggtcc gggggcgggc tcaggggcgg gctcaggg
//
