LOCUS       sequence-341675-       13668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila QF:Gal4 expression under introduced sequence control, 
            PhiC31 transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13668)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 13668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_Gateway>QF:Gal4
FEATURES             Location/Qualifiers
     source          1..13668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4838..5268
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5291..5415
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5440..5445,5446..5463,5464..5470)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5524..6183
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5590..5609)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6525..6830
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6709..6728
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6871..6995)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          7183..7249
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    10728..11158
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    11192..11261
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11480..11498)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11519..11535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11519..11535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11532..11554)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11543..11559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11567..11573,11574..11591,11592..11597))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11612..11633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11750..11767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11921..12509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12001..12020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12680..13471,13472..13540))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13303..13322
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13541..13645)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atctgaattc
     5281 gcttaattaa acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     5341 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     5401 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     5461 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     5521 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     5581 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     5641 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     5701 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     5761 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     5821 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     5881 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     5941 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     6001 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     6061 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     6121 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     6181 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     6241 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     6301 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     6361 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     6421 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     6481 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     6541 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     6601 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     6661 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     6721 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     6781 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     6841 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     6901 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     6961 tacgtttctc gttcagcttt cttgtacaaa gtggtgataa acggccggcc gagctcgccc
     7021 ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg
     7081 aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact
     7141 ttgaatcaca agacgcatac caaacggtac cagatctaaa aggtaggttc aaccactgat
     7201 gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg cggccgcgtt
     7261 taaacaactt aaaaaaaaaa atcaaaatgc cacccaagcg caaaacgctt aacgctgcgg
     7321 ctgaggctaa cgctcatgcc gacggacacg ccgacggaaa cgccgacgga cacgtggcca
     7381 atacggccgc gtcctcgaat aatgcgaggt tcgctgatct cactaacatc gatactccgg
     7441 gtctgggacc cacaactacg accctgctcg tggaaccagc acgctcaaag cgtcaacgag
     7501 tgtcccgcgc atgcgaccag tgccgtgcag cccgagagaa atgcgacgga atacagcctg
     7561 cgtgtttccc gtgcgtttcc cagggaaggt cctgcactta tcaggcttcg ccgaaaaaga
     7621 ggggagttca aaccggttat attcgtacgc tggagctcgc cctcgcctgg atgtttgaaa
     7681 atgtcgcgcg ttccgaagat gccttgcata acctcctcgt ccgtgacgcc ggacaaggat
     7741 cagctctgct cgttggtaaa gattcgccgg ctgccgagcg actccatgcc cgttgggcta
     7801 ctagccgtgt caataagagc attacccgcc tcctcatcga cagtgcggcc caccacgaca
     7861 acagcaccat tccactggac ttcatgcctc gtgacgcact gcatggattt gactggagcg
     7921 aggaggatga tatgagcgac ggactcccat tcctgaagac cgaccccaat aacaatggtt
     7981 tctttggcga cggcagcctg ctgtgtatcc tgcgaagtat tggttttaag ccggaaaatt
     8041 acacgaacag taatgtcaat cgtcttccca caatgatcac cgaccgttac acgctggcca
     8101 gtcgcagtac tacaagccgc ctgctgcaaa gctatcttaa caacttccac ccctactgcc
     8161 ccatagtgca tagtcctact ctcatgatgc tttacaacaa tcagattgag atagcatcga
     8221 aggaccagtg gcagatcctc ttcaactgca ttctcgccat cggcgcttgg tgtattgaag
     8281 gcgagagcac agatatcgac gtgttttact atcaaaacgc caagtcgcac ctcacctcga
     8341 aggtcttcga aagtggaagc attatcctcg tgacagccct ccatctgctg agtcgttata
     8401 cccagtggcg tcagaaaact aatacatcct acaactttca ttcgttcagc attcggatgg
     8461 cgattagcct gggacttaat cgtgacctcc caagtagctt cagcgattcg tcgatcctgg
     8521 aacagcgccg gaggatttgg tggagtgtgt acagctggga gatccagctg tccctcttgt
     8581 acggacgcag cattcagctg agtcagaaca cgatttcctt cccgtccagc gtggacgacg
     8641 ttcaacggac aactacgggc ccgaccatct accacggtat cattgagacg gcccgcttgc
     8701 ttcaagtgtt tacgaagatt tatgagctgg ataagacagt gaccgcggag aagagtccga
     8761 tctgcgccaa gaaatgcctg atgatctgca atgagataga ggaggtgagc cgccaggcac
     8821 ccaagttcct gcagatggac ataagcacta cggcgctgac taatctcctc aaagaacacc
     8881 cctggctgag tttcacccgc ttcgagctga agtggaaaca gctgagtctg attatatacg
     8941 ttttgcggga ctttttcaca aattttacgc agaagaagtc gcagcttgag caagatcaga
     9001 acgaccatca gtcctacgag gtaaagaggt gcagcataat gctctccgac gcagcccagc
     9061 gcaccgtgat gtcggtgtcg tcctatatgg acaaccataa tgtgacccct tatttcgcct
     9121 ggaattgctc ctactatttg ttcaacgccg tgttggtccc aatcaagact cttctgagca
     9181 actccaaatc gaacgccgag aataacgaga cagcacagct cttgcagcaa attaacaccg
     9241 tgttgatgct gttgaagaaa ctcgctacat tcaagattca gacttgcgaa aagtacatcc
     9301 aggtgttgga agaggtgtgc gccccattcc ttctttcgca atgcgcaatt cccctcccgc
     9361 acataagtta caataattcc aacggctccg ctatcaagaa tatcgtcggc tccgctacaa
     9421 tcgcgcagta tcccacgctg cctgaggaaa atgttaacaa catatcggtg aagtatgtaa
     9481 gtccaggcag cgtcggcccg agccctgtgc cgctgaaaag cggagcctcc ttcagcgatc
     9541 tggtcaagct gctcagcaac cgcccaccat cccgcaactc ccctgtcact ataccccgat
     9601 cgaccccgtc gcatcgtagc gttaccccat tcctgggaca acagcagcaa ctgcagagtc
     9661 tggtgccact tacgccctcg gcattgtttg gcggagccaa cttcaaccag agtggtaaca
     9721 ttgcggacag ctccttgagc ttcacgttta ccaacagctc caacggtcca aacttgatca
     9781 cgactcagac aaactcccag gcattgtccc agcccattgc cagctcgaat gtgcatgata
     9841 actttatgaa taatgaaatt actgcctcca agattgacga cggcaacaac agcaagcccc
     9901 tttccccagg ctggactgac cagacggcct acaatgcttt cggtatcacc actggcatgt
     9961 tcaacaccac aacgatggac gacgtatata actatctttt tgatgatgag gatactccac
    10021 cgaacccaaa gaaggagtag cctgcaggaa tgaatcgttt ttaaaataac aaatcaattg
    10081 ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt aggaagatta
    10141 cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac gccacaattg
    10201 tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct ttgttcatag
    10261 acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg tatagcgagc
    10321 tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt tcatcaccct
    10381 cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca taatctaata
    10441 aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact
    10501 cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca aagtacgcag
    10561 tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg ttgtaaccga
    10621 acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc acgatgccga
    10681 caatggagct ggcttggata gcgattcgag ttaactctag aagatcttgg ccacgtaata
    10741 agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt
    10801 gcatacctta tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat
    10861 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata
    10921 ctttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt
    10981 ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa
    11041 caaattttcg ttgcataccc aataaaagat tattatattg catacctttt cttgccatac
    11101 catttagccg atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacgg
    11161 atccactagt gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg
    11221 tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatgaacg
    11281 ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag ccctcgccct
    11341 cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg
    11401 cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg tcgacactag
    11461 ttctagccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt
    11521 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11581 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    11641 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    11701 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    11761 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11821 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11881 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11941 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12001 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12061 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12121 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12181 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12241 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12301 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12361 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12421 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12481 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12541 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12601 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12661 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12721 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12781 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12841 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    12901 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    12961 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    13021 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13081 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13141 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13201 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13261 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13321 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13381 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13441 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13501 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13561 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13621 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
