LOCUS       sequence-341678-       12063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila LexA:QF expression under introduced sequence control, 
            PhiC31 transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12063)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_Gateway>LexA:QF
FEATURES             Location/Qualifiers
     source          1..12063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4838..5268
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5291..5415
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5440..5445,5446..5463,5464..5470)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5524..6183
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5590..5609)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6525..6830
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6709..6728
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6871..6995)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          7183..7249
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7293..7313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7323..7574,7575..7928)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     protein_bind    9123..9553
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    9587..9656
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9875..9893)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9914..9930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9914..9930)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9927..9949)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9938..9954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9962..9968,9969..9986,9987..9992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10007..10028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10145..10162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10316..10904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10396..10415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11075..11866,11867..11935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11698..11717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11936..12040)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atctgaattc
     5281 gcttaattaa acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     5341 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     5401 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     5461 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     5521 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     5581 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     5641 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     5701 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     5761 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     5821 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     5881 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     5941 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     6001 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     6061 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     6121 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     6181 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     6241 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     6301 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     6361 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     6421 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     6481 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     6541 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     6601 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     6661 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     6721 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     6781 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     6841 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     6901 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     6961 tacgtttctc gttcagcttt cttgtacaaa gtggtgataa acggccggcc gagctcgccc
     7021 ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg
     7081 aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact
     7141 ttgaatcaca agacgcatac caaacggtac cagatctaaa aggtaggttc aaccactgat
     7201 gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg cggccgcgtt
     7261 taaacaactt aaaaaaaaaa atcaaaatgc cacccaagaa gaagcgaaaa gtagaagatc
     7321 caatgaaggc tctcacggcc cgacaacagg aagtttttga tttgatacgg gatcatatat
     7381 cccaaacggg tatgcctccg acccgcgcag agatagcaca gcgactgggc tttcgatcgc
     7441 ctaacgccgc ggaggagcac ttgaaggcac tggcccgcaa gggtgtcatt gaaatcgtgt
     7501 ccggtgcgag ccgcggaatc cggctgttgc aggaggagga agagggcctg ccactggtgg
     7561 gacgcgtggc cgctggcgag ccgctgctgg cccagcaaca catagaggga cactatcagg
     7621 tggacccctc cttgtttaag ccaaatgctg atttcctgtt gcgggtgtcg ggaatgtcca
     7681 tgaaggacat cggtattatg gatggtgacc tcctcgccgt ccataagaca caggatgtca
     7741 ggaacggcca ggtagtcgtt gccaggatag acgatgaggt cactgtgaaa cgtctcaaga
     7801 agcaaggcaa taaggtcgag ctgctgccgg agaatagcga gttcaagccg atcgtggtgg
     7861 atctgcgaca gcagtccttt actatcgagg gcttggccgt gggtgtgatc cgcaacggag
     7921 attggctggg acgtcagttg gagctccctc ctaccgccac ggctacggcc tcgataatgc
     7981 cgcacgtgat ggagcagcct ctcagtacca gcattaaccc cgtcaacgac cgcttcaacg
     8041 gtattcccaa ccccactccg tataactccg atgcagctct cgatgctatc actcagacca
     8101 acgattatgg aagcgtaaat acacatggta tcctctctac ttacccgcca ccggctacgc
     8161 accttaatga agcttccgtc gctctcgctc ccggtggcgc ccccccccga ccgcctcctc
     8221 cgtatgttga cagcacgacc aatcacccgc cgtaccactc gaatctggtt ccaatggcga
     8281 actttggtta ctcgaccgtt gattacgatg ccatggttga cgatttggct agcattgaat
     8341 acacggacgc tgtggatgtc gacccacagt ttatgaccaa tctgggattc gttcctggat
     8401 gtaacttctc cgacattaat acatacgaac agtgacctgc aggaatgaat cgtttttaaa
     8461 ataacaaatc aattgtttta taatattcgt acgattcttt gattatgtaa taaaatgtga
     8521 tcattaggaa gattacgaaa aatataaaaa atatgagttc tgtgtgtata acaaatgctg
     8581 taaacgccac aattgtgttt gttgcaaata aacccatgat tatttgatta aaattgttgt
     8641 tttctttgtt catagacaat agtgtgtttt gcctaaacgt gtactgcata aactccatgc
     8701 gagtgtatag cgagctagtg gctaacgctt gccccaccaa agtagattcg tcaaaatcct
     8761 caatttcatc accctcctcc aagtttaaca tttggccgtc ggaattaact tctaaagatg
     8821 ccacataatc taataaatga aatagagatt caaacgtggc gtcatcgtcc gtttcgacca
     8881 tttccgaaaa gaactcgggc ataaactcta tgatttctct ggacgtggtg ttgtcgaaac
     8941 tctcaaagta cgcagtcagg aacgtgcgcg acatgtcgtc gggaaactcg cgcggaaaca
     9001 tgttgttgta accgaacggg tcccatagcg ccaaaaccaa atctgccagc gtcaatagaa
     9061 tgagcacgat gccgacaatg gagctggctt ggatagcgat tcgagttaac tctagaagat
     9121 cttggccacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat attttcggca
     9181 aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt ttagagaaac
     9241 caaataattt tttattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     9301 aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     9361 atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg
     9421 catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac
     9481 cttttcttgc cataccattt agccgatcaa ttgtgctcgg caacagtata tttgtggtgt
     9541 gccaaccaac aacggatcca ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt
     9601 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
     9661 ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg
     9721 ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga
     9781 cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg
     9841 gcatgtcgac actagttcta gccagctttt gttcccttta gtgagggtta atttcgagct
     9901 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9961 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10021 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10081 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10201 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10261 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10321 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10381 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10441 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10501 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10561 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10621 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10681 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10741 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10801 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10861 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10921 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10981 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11041 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11101 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11161 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11221 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11281 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11341 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11401 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11461 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11521 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11581 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11641 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11701 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11761 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11821 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11881 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11941 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12001 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12061 cac
//
