LOCUS       sequence-341693-        8923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4958
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     414..431
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    548..569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(584..589,590..607,608..614)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    622..638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     627..649
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     646..662
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..662
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1881..2594
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1911..1939)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2534..2554
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      4019..4206
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4074..4098)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4864..6439
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        4864..5207
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5208..6236
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             5208..6236
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      6242..6439
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6493..6510)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6493..6509)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6502..6524)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6722..7177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6809..6828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7019..7040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7194..7213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7373..7391)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7459..7563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7564..7632,7633..8424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7782..7801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8595..8923,1..260)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
       61 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      301 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      361 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      421 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      481 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      541 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      601 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      661 accatgatta cgccaagctt gcatgcctgc aggtcgatcg actctagagg atcagaaaat
      721 tatcgccata aaagacagaa taagtcatca gcggttgttt catttcctat attttttttt
      781 tattttttta ttttttaata agggaaaatt taacgtctaa ggatacagaa gattgttagc
      841 acattaaagt aataaaggct taagtagtaa gtgccttagc atgttattgt atttcaaagg
      901 acataatcta aaataataac aatatcattt ctcacaagtt attcaatttt cttttttttt
      961 tctaataata tcaagaatgt attatttgtt tgacataagt caactaattt atttaatatg
     1021 ctggattaat cttgcagaca tgtaaattaa caagttttag tcaaataacg ttgaagtttc
     1081 aatgaactca aataatttct cttttttttt atataaccat aatctgattt atattttccg
     1141 cagggatcaa ctgaagttat gacatttgga ttggatcact tataaccttg gtcgccaaat
     1201 aatacaaaaa tcagcgttat aaaacaaaga aggtttttgt taagaaatta atcctctttc
     1261 ttgataagaa agttgaaccg aaattgcaga tactgatata tgaaaataat acccacaatt
     1321 ttgggaatag cgcaagcctc aatttaaaca ataggtgagg acacatgata atgacctcaa
     1381 tgattgttag aagaaaagag cctcattaca aaatcgaaaa atgaatggtt gggtacaagt
     1441 ttccaaaaca tggtaaagtg gactttgcgt atgagacgta aatagaaaaa aacacttgtt
     1501 atatgttttc tagaattatt gttgtctctt tatggttgga tgatgcaaaa tagtaatttc
     1561 ggttagttgc tgtaaaacac cacgagacaa atagatatgg atatttatta aatcaggaaa
     1621 aacgtaactc tcggctactg gatggttcag tcacccaacg attactgggg agagaaaaca
     1681 gggcaaaagc aaagcttaaa ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa
     1741 aaccggataa tggacctgtt aatcgaaaca ttgaagataa aggaagagga atcctggcat
     1801 atcatcaatt gaataagttg aattaattat ttcaatctca ttctcacttt ctgacttata
     1861 gtcgctttgt taaagctagc atgagtaaag gagaagaact tttcactgga gttgtcccaa
     1921 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     1981 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     2041 ctgttccatg gccaacactt gtcactactt tgacttgggg tgttcaatgc ttttcaagat
     2101 acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
     2161 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
     2221 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     2281 gaaacattct tggacacaaa ttggaataca actatatttc acacaatgta tacatcactg
     2341 cagacaaaca aaagaatgga atcaaagctc atttcaaaat tagacacaac attgaagatg
     2401 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     2461 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     2521 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     2581 atgaactata caaaggtgga tccatgcagt atttgtgtca gaggtgtcat tctttgataa
     2641 attttaaaga tgtttacgat gatgacttgt taaagctaaa aaatcttccg aaaagtagat
     2701 ttgttcaagc atcatcgttg acagagatga atgagagtgg ggagagtgat gatcaaatga
     2761 attcttcttc tgaagactat ccagctcaga gattgcaact ttacaaaaaa acaataagcg
     2821 aaggtgatta caattttgat aatgtaccac cgcccgaatt gaggacgccc actttagatt
     2881 catttgttgt attgccagct gctaaagatg gctatgaaga agaaaagaat tcgcctgaag
     2941 aggtgaatga tctttttagt tggaaaatag aaatttataa cagaatattt gatcttttat
     3001 cctctaaaac caaagtcgat caccctcttt gtgtcgaatg tgctgagtta ttgactgaag
     3061 agatgtctaa aacattaagg gccctaaaag aggaaaaaaa aatgtatttt aattatgata
     3121 atttcttatc aagtcaaaca gtgcatgaag aaaatactgc tgcgcttgat tctgagatag
     3181 atgaattaat gaaacagatt aatgagaaag aagaaaagat cgaggaaatt agtgatgaaa
     3241 cagacaaact tcaaaagttg ctacgtgaat tggacgaaga aaaggaaaaa gtttatgccg
     3301 aggagcaaga gttctacaat aatctaaacc aatttcaaat aaaaaagctg agtttagaaa
     3361 gacaatacga ttgtgcaaac ttggagtttg agcataactc taggaagctc gagaagcttc
     3421 agaaaatgaa tgttttcagt gatatttttt acatttctca ttattctgag ccgaatggag
     3481 agggctctat tgctacaatt aatggcttac gcttgggaag gcttccaagc cagaaagtta
     3541 attgggctga gataaatgcc gcttggggga tgactgtttt actattagat gttttgactg
     3601 aaaagttaga ctttcattcc tcttcctatc agctgaagcc ttttggcagc caatctttta
     3661 taattcgttt tgatcgcgat cctaatggaa atcaagttaa accaacaaag ctagatcttt
     3721 tttctagcgg agagctcaaa atatttatga atcgtagatt tgaccaaggc atggtggctt
     3781 ttctggatta cttacatcaa tttggcgatt tttgtgctgc taaaacacct tcagcggttt
     3841 taccctatgc cattgaaaat gaccgtatcg gcggaaaatg catacgttta gcattcaatc
     3901 aggacgagaa ttggactcgt gcgttgaagt tcgtcctaac ggatatcaaa tttttggaag
     3961 cttacgtttc ttctcaggac aagcagtcga acttttaagg cgcgccactt ctaaataagc
     4021 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
     4081 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
     4141 cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc
     4201 taccggcaga tccgctaggg ataacagggt aatatactag tgttggcagc gatgaaatta
     4261 ttgactcctt aattcgcatt tcttgtattc ctggaaaaga taagattttg atgtgtccac
     4321 cttcgtacgg aatgtatacg gtctctgcaa aaatcaacga tgttgaggtt gttaaggttc
     4381 tccttgaacc agatttcaat ttgaacgtcg atgctatttg cgaaacactt tctaaagata
     4441 gtgccatcaa agtctttttt gcttgttccc ctggtaatcc aacagcggcc gcgggcccgc
     4501 ggccgctctt aaactcgagg atattaagaa gattttggag caccctacat ggaatggaat
     4561 cgttgtcgtc gatgaggcct atattgattt ctctgctccc gatatgtctg ccttaactct
     4621 tgtcaatgaa tatccaaacc ttgctgtttg ccaaaccctt tcgaaatctt tcggtctagc
     4681 aggaattcgg taagttaatc taagctttta agcattaata ttatttcttt actaactatg
     4741 atgtttagta tcggattttg tttgacaagt aaacctattg ctaccattat gaactcatta
     4801 aaggctcctt ataacattag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
     4861 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     4921 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     4981 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcggacctgc
     5041 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     5101 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     5161 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaaaagc
     5221 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     5281 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     5341 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     5401 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     5461 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     5521 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     5581 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     5641 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     5701 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     5761 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     5821 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     5881 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     5941 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     6001 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     6061 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     6121 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     6181 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataatcag
     6241 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
     6301 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
     6361 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
     6421 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tgtcgaaaac
     6481 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6541 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6601 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     6661 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     6721 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6781 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6901 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     6961 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7021 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
     7081 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
     7141 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     7201 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     7261 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7321 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     7381 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7561 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7621 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7681 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7741 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7801 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7861 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7921 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7981 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8041 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8101 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8161 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8221 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8281 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8341 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8401 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8461 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8521 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8581 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8641 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8701 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8761 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8821 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8881 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gtt
//
