LOCUS       sequence-341694-       10189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4658
FEATURES             Location/Qualifiers
     source          1..10189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1859..2572
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2971..3158
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3026..3050)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4822..5616)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      6225..7008
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7731..7748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7731..7747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7740..7762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7960..8415
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8047..8066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8257..8278
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8432..8451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8551..8573
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8611..8629)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8697..8801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8802..8870,8871..9662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9020..9039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9833..10189,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct
      661 gcaggtcgat cgactctaga ggatcagaaa attatcgcca taaaagacag aataagtcat
      721 cagcggttgt ttcatttcct atattttttt tttatttttt tattttttaa taagggaaaa
      781 tttaacgtct aaggatacag aagattgtta gcacattaaa gtaataaagg cttaagtagt
      841 aagtgcctta gcatgttatt gtatttcaaa ggacataatc taaaataata acaatatcat
      901 ttctcacaag ttattcaatt ttcttttttt tttctaataa tatcaagaat gtattatttg
      961 tttgacataa gtcaactaat ttatttaata tgctggatta atcttgcaga catgtaaatt
     1021 aacaagtttt agtcaaataa cgttgaagtt tcaatgaact caaataattt ctcttttttt
     1081 ttatataacc ataatctgat ttatattttc cgcagggatc aactgaagtt atgacatttg
     1141 gattggatca cttataacct tggtcgccaa ataatacaaa aatcagcgtt ataaaacaaa
     1201 gaaggttttt gttaagaaat taatcctctt tcttgataag aaagttgaac cgaaattgca
     1261 gatactgata tatgaaaata atacccacaa ttttgggaat agcgcaagcc tcaatttaaa
     1321 caataggtga ggacacatga taatgacctc aatgattgtt agaagaaaag agcctcatta
     1381 caaaatcgaa aaatgaatgg ttgggtacaa gtttccaaaa catggtaaag tggactttgc
     1441 gtatgagacg taaatagaaa aaaacacttg ttatatgttt tctagaatta ttgttgtctc
     1501 tttatggttg gatgatgcaa aatagtaatt tcggttagtt gctgtaaaac accacgagac
     1561 aaatagatat ggatatttat taaatcagga aaaacgtaac tctcggctac tggatggttc
     1621 agtcacccaa cgattactgg ggagagaaaa cagggcaaaa gcaaagctta aaggaatccg
     1681 attgtcattc ggcaatgtgc agcgaaacta aaaaccggat aatggacctg ttaatcgaaa
     1741 cattgaagat aaaggaagag gaatcctggc atatcatcaa ttgaataagt tgaattaatt
     1801 atttcaatct cattctcact ttctgactta tagtcgcttt gttaaagcta gcggatccat
     1861 gtctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
     1921 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
     1981 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
     2041 cactactttc ggttatggtg ttcaatgttt tgcgagatac ccagatcata tgaaacaaca
     2101 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
     2161 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
     2221 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
     2281 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
     2341 caaagttaac ttcaaaatta gacacaacat tgaagatggt tctgttcaat tagctgacca
     2401 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
     2461 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
     2521 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aaagatctat
     2581 gcgttctcaa ttcaaggacg acttttcttt tgagaaacga aaaacagagt ctcaaagaat
     2641 ccgggaaaaa tatcccgatc gaatacctgt tatttgcgag aaagttgata aatctgatat
     2701 tgctgcaata gataaaaaga aatatctcgt tccatctgac cttacagttg gtcaatttgt
     2761 atatgttatt cgcaaacgga taaaactttc tcctgagaag gccatcttta ttttcattga
     2821 tgaaatcttg cccccgacag cggccttgat gtcaacgata tacgaagagc ataaaagtga
     2881 ggacggcttt ttgtatatca cgtattcagg agaaaataca tttggaacag tgtttccttt
     2941 ttagagatct ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
     3001 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     3061 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     3121 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
     3181 gtaatatact agtaagatga ttcttttatc gtcgataacg atgaggatgt agacgattat
     3241 gatgagagtg attagcttct aaaatttggt gttttcaaac gctatttaca gtacaatctg
     3301 aaaaaattgg atgaacattg taaatggtag gatacgagtt ccaattaatg tattttttaa
     3361 gtattacact tagcttatta attttatata tacattctac ctctgtgggt ttaaagacaa
     3421 ctcgtacaca tactcaaatt atttatgaac attataatgc atattcgtac tgtctattaa
     3481 acattttttt gacattaaaa atttcgttta ctaacgtaga aagcgctgat aattgaggct
     3541 actattttaa tatctagaaa caaagattca ttaattaatt aagtaagctc caggatactt
     3601 gtatatttcg ttaaaaaagc gcaatttcaa caattcctat gaacatccat gatttcatca
     3661 caaaatcaaa catatcagga tttaacgtga attatttgct ttcttgtgat tttcatctga
     3721 tttctggtca tttacgttac tgtaaacaag ctttttaaaa aaaggatttt ttacgcaata
     3781 ttatcgatat cccaatctgt agtataaaaa gtgcaacagt gttgagtttc ccgaaaccag
     3841 gcatttttgc ggtagggtct gaaactttca cgaaaccatt tcacaacaaa acttcttcaa
     3901 caacatgtgt gcaaagaaaa tcgtcgtctt accaggagac catattggcc ctgaaattgt
     3961 tgcttctgcc ttggaggttt tgaaagtcgt tgagaagaag cgacctgagt taaaactcga
     4021 gtttgaagaa cacaagattg gaggtgcctc tattgatgcc tatggaaccc ctttgactga
     4081 cgagactgtg aaggcttgtt tggaagctga cggtgttctt ttgggtgccg ttggtggtcc
     4141 tgaatggacc aaccccaatt gtcgtcctga gcaaggttta ttgaagcttc gtaagagtat
     4201 gggtgtttgg gccaaccttc gaccttgcaa ctttgccagc aagtctttag tcaagtacag
     4261 ccctttgaag cctgaaatcg ttgaaggtgt cgatttttgt gttgtacgag aacttactgg
     4321 aggttgttac tttggtgagc gcactgagga caacggatcg ggttatgcta tggacacttg
     4381 gccttacagt ttggaagaag tttctcgtat tgctcgtttg gcggccgcgg gcccaacacc
     4441 aatgtttata accaagtttt atcttgtttg tctacatggt attttacatt catctacata
     4501 catctttcat tggctttgta catagttatc attacaagtc taaaaaaatt cactcttttc
     4561 ttattcaatg tcaatccaag agaaaagatt gtggtaatgt tgtaggagca tgtttaataa
     4621 attactatag caaattactt tttattccca aggtgtttat ctataatagt taatatttta
     4681 gtcgctacat aaaattttac caaagagtac ttgtatacta attctaaatg ccttctgaca
     4741 taaaacgcct aggaaaacaa acgcaaacaa ggcatcgact ttttcaataa ccaaccaaaa
     4801 aaattttaca ttagtctttt tttaatgctg agaaagtctt tgctgatatg ccttccaacc
     4861 agcttctcta tatctcttgg cttcgacaac aggattacga ccagctccat agactccacg
     4921 accaacaatg atgatgtcgc taccgcagtt tacaatcact tcttcaggag tacgatattg
     4981 ctgtcccagc ccgtctcctt taacatccaa gccgatacca ggggacatag ttatgtagtc
     5041 gctttgaagg ttaggaaatc gacgaccagc tataaagcca aagcaaaaat cggtatgctt
     5101 ctcaaaccat tctaaggttt tctctgtgta ggaaccagta gccaaagagc ctttggaaga
     5161 catttcagcc aaaagcaaga gaccacgtcc caaaggtaaa ccaacttctt tgaggccttg
     5221 tataataccc tcgcctggca ctgtatggca atttgtgata tgagcccaag aagcaatttt
     5281 gtacacacca gatgcatatt gtagcttgac ggtatttcca atgtctgcga atttgcgatc
     5341 ctcaaagata agaaaacgat gctttttacc taaggccacc agtttttcta ccatatcctg
     5401 gtcgaaatcc tcgacaacgt caatatgtgt cttgataaca cagacatagg gtccaatttt
     5461 atctaccaat tctaagattt cggatttctt cgtcaaatcg accgcgactg acaagttgct
     5521 ttgcttttct tccatcaaag ccaacaattc cttggcaatg ggatttttca tcccctcagc
     5581 tctagctgaa tagctttgaa atactctagc atccataact ttgcttttaa acctttaatt
     5641 tcgatccaag caaaaaagag gttcttggta ggacaatacg gtaagaaaac acgacatgtg
     5701 cagagatgcc gacgaagcat agttaaactg ggatggtaaa atcaattaag aatttataaa
     5761 gacaaaattg tataagtctc taaaacatct taattatacc tcacagaact atctaaaata
     5821 tattcacaaa gtgcaaacat tatcatgaaa aagaaccatt ttaatttaaa gcaagggcat
     5881 taaggcttat ttacagaatt tcttactttt gtaaagatta taaggctgat tatctttttc
     5941 accatgccaa aaattacaca agatagaatg gatgtttgaa attaaacgtg agtatacaaa
     6001 caaatacact aggtaaatcg aaacattttt ttctccatta agtaacaaat tcctatttag
     6061 agaaagaatg ctgagtagat taaataatct atacaaactt ttttaacaca aatgcataca
     6121 tatagccagt gggatttgta gctaagcttg cgaattcgag tctaactcct taaccactcg
     6181 gacatagtga cttatctgac actcattgta aattaatata tatataggca ttttgtttag
     6241 ttaaaggtac ttaagtaatt agtataaacg aaccaatttt ataatcagga agttaagtga
     6301 atggtagcac atgtcgtaaa aattgtgaat ttttattgaa taatatttta aatacaagcc
     6361 tttctagact aggtatactc ataaacatat atgagcaaaa ggatagagga gattacattg
     6421 catcttctac aaatttattt attgcccttt actgaaaaat taaataatga gtactaaatg
     6481 ataaaaagcg ctcagtacaa gaaagattgc aaaaatattg cattcttcat gaattaaagt
     6541 tgcatataag gcatattgaa agtaatagta ctaaaacagc agttagcgaa aattaataga
     6601 attatattcg caagacaatt gtgacattaa attaaaaaat tgtagaattt ttactatcct
     6661 ctttaacgcc atgagccttt ataaaaaggt taaattagtt ttaacattct ttttttgagt
     6721 aagagtttac aatttatcaa aacctgtgtt attatattca ttagtttcaa tttattagca
     6781 tctagagaaa aatcaattgg cagttacatt gttggaattt atgaagaaaa agaatctaca
     6841 acggagaata agttgctgat cgctttccct aaaattgtat attttgctga gcttattttg
     6901 acatttcgtt gaagttttct ctaattcgca ttcattttaa gtaaaacaat gagaaataaa
     6961 attacaaaaa atacaaatta aaatacaatt tttagctata attatagacg atgcccttgt
     7021 atcccattct gtctcgcttg cccctacttt ttatctttta tataccataa tgaacgctgc
     7081 cgctactaac cataccccga ttttacattt cggactccca aggacgtaca aaatagaaaa
     7141 ctatagaaaa aaataatcag aaaatagcat gtcatctctt tgtaaaacgc gtttgcaaga
     7201 agaaaggaaa caatggagaa gagatcatcc atttgtatgt aaattttagt aaacttgaag
     7261 aaatcactaa caacttctct tacttaggga ttctatgcaa aaccttgtaa atcatctgat
     7321 ggaggactcg atttaatgaa ttggaaggtt gggcccgcgg ccgcttagct gaaacttcca
     7381 accctcctgc tcccgtcaca ttactcgaca aagctaatgt tttggcaact tctcgtcttt
     7441 ggcgcaagac tgtagctaag atttttaagg aggaatatcc tcaccttacc ttaaaaaacc
     7501 agctcattga ctccgcagcc atgcttttgg tcaagagccc tcgtacactt aacggtgttg
     7561 ttttgactga caacttgttt ggtgacatta tttcagatga ggcttctgtc attcctggta
     7621 gcttgggcct tttgccttct gcctcccttt ccggtgtggt aggaaaatca gaagaaaagg
     7681 ttcattgttt ggttgagccc attcacggta gcgctcccga gctcgaattc actggccgtc
     7741 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7801 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7861 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     7921 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     7981 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     8041 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8101 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8161 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8221 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8281 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     8341 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8401 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8461 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8521 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8581 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8641 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8821 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8881 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8941 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9001 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9061 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9121 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9181 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9241 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9301 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9361 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9421 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9481 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9541 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9601 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9661 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9721 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9781 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9841 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9901 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9961 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10021 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10081 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10141 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaac
//
