LOCUS       sequence-341695-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4925
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2505..3212
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2650..2668)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2918..2937
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3341..3528
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3396..3420)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5192..5986)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      6595..7378
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(8101..8118)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8101..8117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8110..8132)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8330..8785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8417..8436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8627..8648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8802..8821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8921..8943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8981..8999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9067..9171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9172..9240,9241..10032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9390..9409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10203..10791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10692..10711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10945..10962
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgatcga ctctagagga tcagaaaatt atcgccataa aagacagaat
      301 aagtcatcag cggttgtttc atttcctata tttttttttt atttttttat tttttaataa
      361 gggaaaattt aacgtctaag gatacagaag attgttagca cattaaagta ataaaggctt
      421 aagtagtaag tgccttagca tgttattgta tttcaaagga cataatctaa aataataaca
      481 atatcatttc tcacaagtta ttcaattttc tttttttttt ctaataatat caagaatgta
      541 ttatttgttt gacataagtc aactaattta tttaatatgc tggattaatc ttgcagacat
      601 gtaaattaac aagttttagt caaataacgt tgaagtttca atgaactcaa ataatttctc
      661 ttttttttta tataaccata atctgattta tattttccgc agggatcaac tgaagttatg
      721 acatttggat tggatcactt ataaccttgg tcgccaaata atacaaaaat cagcgttata
      781 aaacaaagaa ggtttttgtt aagaaattaa tcctctttct tgataagaaa gttgaaccga
      841 aattgcagat actgatatat gaaaataata cccacaattt tgggaatagc gcaagcctca
      901 atttaaacaa taggtgagga cacatgataa tgacctcaat gattgttaga agaaaagagc
      961 ctcattacaa aatcgaaaaa tgaatggttg ggtacaagtt tccaaaacat ggtaaagtgg
     1021 actttgcgta tgagacgtaa atagaaaaaa acacttgtta tatgttttct agaattattg
     1081 ttgtctcttt atggttggat gatgcaaaat agtaatttcg gttagttgct gtaaaacacc
     1141 acgagacaaa tagatatgga tatttattaa atcaggaaaa acgtaactct cggctactgg
     1201 atggttcagt cacccaacga ttactgggga gagaaaacag ggcaaaagca aagcttaaag
     1261 gaatccgatt gtcattcggc aatgtgcagc gaaactaaaa accggataat ggacctgtta
     1321 atcgaaacat tgaagataaa ggaagaggaa tcctggcata tcatcaattg aataagttga
     1381 attaattatt tcaatctcat tctcactttc tgacttatag tcgctttgtt aaagctagcg
     1441 gatccatgat gaaccgtgcg tactctttaa gaaacagccg tgcccctacg gcctcacaat
     1501 tgttaaaccc ccctcctccc tcctcttcca ctcgtagtgg tcgtttgttt gcccccttga
     1561 gtcataccat gcgtatcaac cacgctgcca ccttcacccc tgacttggct aagcgtttgg
     1621 ctgtcttggt caagatggaa aagaacgtga tgcgcagtat ggaggtcacc atccgtggcc
     1681 gtcgtgattg tgctcgccaa ctttcctact ggggtgagga ctgcgatgac gatatcagcg
     1741 acgtcaccga caaattgggt gtcttgtttt acgagatggc cgagttggag aactacttga
     1801 tcgatcgtta tgaccaatac cgtatgacgt tgaagtccat ccgtaacatc gagtcctctg
     1861 tccaaccctc tcgtgagaag aagcaaaagc tcttggatca aatctatgct cttaagcata
     1921 aggatcctga atcccctcgc ttggtcacca tggagcaaga gcttgttcgt gaggaggctg
     1981 cctgtttggt tgccgaggct caactttcca acaccacccg tgaaaagttc aagcaagcca
     2041 tgaccttcaa cttggatgcc ctccacgagc atgccgagaa gctcaacctc attgccacct
     2101 atggcagaca tttgttgaac ttgattgacg acacccccgt cactcccggt gaggctcgtc
     2161 ctgcctacga tggttatgag acttcccgcc aaatcgtcat ggatgctgag cacgccttga
     2221 gctcttgggt tcccagccaa cccgccgtca actttgtcaa gcctcaaatc gaggatgatg
     2281 cccaaagtga cgctcgttcc tggaacgagt atgaggctca aggtgagccc gtccccgttg
     2341 agcaagtcac ccacttacaa gacgacgttc aatccgacac tagcgagatc attgagaacg
     2401 agcctggcat gcaccctcat gtccatgctg agcgtatgtc tcgcattgct tctgagtcta
     2461 gcgatgccgt tcctcagcaa accgccgtcc aggttgctgg atccatggtg agcaagggcg
     2521 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     2581 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2641 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2701 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2761 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2821 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2881 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2941 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3001 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3061 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     3121 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     3181 actccaccgg cggcatggac gagctgtaca agggtagatc tcccatggat gatgatcagc
     3241 catcaccaag catatttagc tccgatgaga gttttttgat cgtagaaggt gatgattttt
     3301 ctcttggtaa ctaaagatct ggcgcgccac ttctaaataa gcgaatttct tatgatttat
     3361 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     3421 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     3481 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
     3541 ggataacagg gtaatatact agtaagatga ttcttttatc gtcgataacg atgaggatgt
     3601 agacgattat gatgagagtg attagcttct aaaatttggt gttttcaaac gctatttaca
     3661 gtacaatctg aaaaaattgg atgaacattg taaatggtag gatacgagtt ccaattaatg
     3721 tattttttaa gtattacact tagcttatta attttatata tacattctac ctctgtgggt
     3781 ttaaagacaa ctcgtacaca tactcaaatt atttatgaac attataatgc atattcgtac
     3841 tgtctattaa acattttttt gacattaaaa atttcgttta ctaacgtaga aagcgctgat
     3901 aattgaggct actattttaa tatctagaaa caaagattca ttaattaatt aagtaagctc
     3961 caggatactt gtatatttcg ttaaaaaagc gcaatttcaa caattcctat gaacatccat
     4021 gatttcatca caaaatcaaa catatcagga tttaacgtga attatttgct ttcttgtgat
     4081 tttcatctga tttctggtca tttacgttac tgtaaacaag ctttttaaaa aaaggatttt
     4141 ttacgcaata ttatcgatat cccaatctgt agtataaaaa gtgcaacagt gttgagtttc
     4201 ccgaaaccag gcatttttgc ggtagggtct gaaactttca cgaaaccatt tcacaacaaa
     4261 acttcttcaa caacatgtgt gcaaagaaaa tcgtcgtctt accaggagac catattggcc
     4321 ctgaaattgt tgcttctgcc ttggaggttt tgaaagtcgt tgagaagaag cgacctgagt
     4381 taaaactcga gtttgaagaa cacaagattg gaggtgcctc tattgatgcc tatggaaccc
     4441 ctttgactga cgagactgtg aaggcttgtt tggaagctga cggtgttctt ttgggtgccg
     4501 ttggtggtcc tgaatggacc aaccccaatt gtcgtcctga gcaaggttta ttgaagcttc
     4561 gtaagagtat gggtgtttgg gccaaccttc gaccttgcaa ctttgccagc aagtctttag
     4621 tcaagtacag ccctttgaag cctgaaatcg ttgaaggtgt cgatttttgt gttgtacgag
     4681 aacttactgg aggttgttac tttggtgagc gcactgagga caacggatcg ggttatgcta
     4741 tggacacttg gccttacagt ttggaagaag tttctcgtat tgctcgtttg gcggccgcgg
     4801 gcccaacacc aatgtttata accaagtttt atcttgtttg tctacatggt attttacatt
     4861 catctacata catctttcat tggctttgta catagttatc attacaagtc taaaaaaatt
     4921 cactcttttc ttattcaatg tcaatccaag agaaaagatt gtggtaatgt tgtaggagca
     4981 tgtttaataa attactatag caaattactt tttattccca aggtgtttat ctataatagt
     5041 taatatttta gtcgctacat aaaattttac caaagagtac ttgtatacta attctaaatg
     5101 ccttctgaca taaaacgcct aggaaaacaa acgcaaacaa ggcatcgact ttttcaataa
     5161 ccaaccaaaa aaattttaca ttagtctttt tttaatgctg agaaagtctt tgctgatatg
     5221 ccttccaacc agcttctcta tatctcttgg cttcgacaac aggattacga ccagctccat
     5281 agactccacg accaacaatg atgatgtcgc taccgcagtt tacaatcact tcttcaggag
     5341 tacgatattg ctgtcccagc ccgtctcctt taacatccaa gccgatacca ggggacatag
     5401 ttatgtagtc gctttgaagg ttaggaaatc gacgaccagc tataaagcca aagcaaaaat
     5461 cggtatgctt ctcaaaccat tctaaggttt tctctgtgta ggaaccagta gccaaagagc
     5521 ctttggaaga catttcagcc aaaagcaaga gaccacgtcc caaaggtaaa ccaacttctt
     5581 tgaggccttg tataataccc tcgcctggca ctgtatggca atttgtgata tgagcccaag
     5641 aagcaatttt gtacacacca gatgcatatt gtagcttgac ggtatttcca atgtctgcga
     5701 atttgcgatc ctcaaagata agaaaacgat gctttttacc taaggccacc agtttttcta
     5761 ccatatcctg gtcgaaatcc tcgacaacgt caatatgtgt cttgataaca cagacatagg
     5821 gtccaatttt atctaccaat tctaagattt cggatttctt cgtcaaatcg accgcgactg
     5881 acaagttgct ttgcttttct tccatcaaag ccaacaattc cttggcaatg ggatttttca
     5941 tcccctcagc tctagctgaa tagctttgaa atactctagc atccataact ttgcttttaa
     6001 acctttaatt tcgatccaag caaaaaagag gttcttggta ggacaatacg gtaagaaaac
     6061 acgacatgtg cagagatgcc gacgaagcat agttaaactg ggatggtaaa atcaattaag
     6121 aatttataaa gacaaaattg tataagtctc taaaacatct taattatacc tcacagaact
     6181 atctaaaata tattcacaaa gtgcaaacat tatcatgaaa aagaaccatt ttaatttaaa
     6241 gcaagggcat taaggcttat ttacagaatt tcttactttt gtaaagatta taaggctgat
     6301 tatctttttc accatgccaa aaattacaca agatagaatg gatgtttgaa attaaacgtg
     6361 agtatacaaa caaatacact aggtaaatcg aaacattttt ttctccatta agtaacaaat
     6421 tcctatttag agaaagaatg ctgagtagat taaataatct atacaaactt ttttaacaca
     6481 aatgcataca tatagccagt gggatttgta gctaagcttg cgaattcgag tctaactcct
     6541 taaccactcg gacatagtga cttatctgac actcattgta aattaatata tatataggca
     6601 ttttgtttag ttaaaggtac ttaagtaatt agtataaacg aaccaatttt ataatcagga
     6661 agttaagtga atggtagcac atgtcgtaaa aattgtgaat ttttattgaa taatatttta
     6721 aatacaagcc tttctagact aggtatactc ataaacatat atgagcaaaa ggatagagga
     6781 gattacattg catcttctac aaatttattt attgcccttt actgaaaaat taaataatga
     6841 gtactaaatg ataaaaagcg ctcagtacaa gaaagattgc aaaaatattg cattcttcat
     6901 gaattaaagt tgcatataag gcatattgaa agtaatagta ctaaaacagc agttagcgaa
     6961 aattaataga attatattcg caagacaatt gtgacattaa attaaaaaat tgtagaattt
     7021 ttactatcct ctttaacgcc atgagccttt ataaaaaggt taaattagtt ttaacattct
     7081 ttttttgagt aagagtttac aatttatcaa aacctgtgtt attatattca ttagtttcaa
     7141 tttattagca tctagagaaa aatcaattgg cagttacatt gttggaattt atgaagaaaa
     7201 agaatctaca acggagaata agttgctgat cgctttccct aaaattgtat attttgctga
     7261 gcttattttg acatttcgtt gaagttttct ctaattcgca ttcattttaa gtaaaacaat
     7321 gagaaataaa attacaaaaa atacaaatta aaatacaatt tttagctata attatagacg
     7381 atgcccttgt atcccattct gtctcgcttg cccctacttt ttatctttta tataccataa
     7441 tgaacgctgc cgctactaac cataccccga ttttacattt cggactccca aggacgtaca
     7501 aaatagaaaa ctatagaaaa aaataatcag aaaatagcat gtcatctctt tgtaaaacgc
     7561 gtttgcaaga agaaaggaaa caatggagaa gagatcatcc atttgtatgt aaattttagt
     7621 aaacttgaag aaatcactaa caacttctct tacttaggga ttctatgcaa aaccttgtaa
     7681 atcatctgat ggaggactcg atttaatgaa ttggaaggtt gggcccgcgg ccgcttagct
     7741 gaaacttcca accctcctgc tcccgtcaca ttactcgaca aagctaatgt tttggcaact
     7801 tctcgtcttt ggcgcaagac tgtagctaag atttttaagg aggaatatcc tcaccttacc
     7861 ttaaaaaacc agctcattga ctccgcagcc atgcttttgg tcaagagccc tcgtacactt
     7921 aacggtgttg ttttgactga caacttgttt ggtgacatta tttcagatga ggcttctgtc
     7981 attcctggta gcttgggcct tttgccttct gcctcccttt ccggtgtggt aggaaaatca
     8041 gaagaaaagg ttcattgttt ggttgagccc attcacggta gcgctcccga gctcgaattc
     8101 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8161 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8221 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     8281 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     8341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8401 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8521 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     8581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8641 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     8701 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8761 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     8821 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8881 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8941 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     9001 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     9061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10321 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
