LOCUS       sequence-341698-       12052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4946
FEATURES             Location/Qualifiers
     source          1..12052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        72..176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(177..245,246..1037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(395..414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1208..1796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1697..1716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1950..1967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2084..2105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2120..2125,2126..2143,2144..2150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2158..2174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2163..2185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2182..2198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2182..2198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4482..5189
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4627..4645)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4895..4914
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(5237..5248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      6398..6585
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6453..6477)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8249..9043)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      9652..10435
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(11158..11175)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11158..11174)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11167..11189)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11387..11842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11474..11493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11684..11705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11859..11878)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11978..12000
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(join(12038..12052,1..4))
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
       61 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      181 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      241 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      301 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      361 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      421 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      481 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      541 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      601 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      661 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      721 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      781 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      841 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      901 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      961 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1021 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1201 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1261 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1321 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1381 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1441 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1501 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1561 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1621 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1681 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1741 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1801 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1861 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1921 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1981 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2041 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2101 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2161 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat
     2221 gcctgcaggt cgatcgactc tagaggatca gaaaattatc gccataaaag acagaataag
     2281 tcatcagcgg ttgtttcatt tcctatattt tttttttatt tttttatttt ttaataaggg
     2341 aaaatttaac gtctaaggat acagaagatt gttagcacat taaagtaata aaggcttaag
     2401 tagtaagtgc cttagcatgt tattgtattt caaaggacat aatctaaaat aataacaata
     2461 tcatttctca caagttattc aattttcttt tttttttcta ataatatcaa gaatgtatta
     2521 tttgtttgac ataagtcaac taatttattt aatatgctgg attaatcttg cagacatgta
     2581 aattaacaag ttttagtcaa ataacgttga agtttcaatg aactcaaata atttctcttt
     2641 ttttttatat aaccataatc tgatttatat tttccgcagg gatcaactga agttatgaca
     2701 tttggattgg atcacttata accttggtcg ccaaataata caaaaatcag cgttataaaa
     2761 caaagaaggt ttttgttaag aaattaatcc tctttcttga taagaaagtt gaaccgaaat
     2821 tgcagatact gatatatgaa aataataccc acaattttgg gaatagcgca agcctcaatt
     2881 taaacaatag gtgaggacac atgataatga cctcaatgat tgttagaaga aaagagcctc
     2941 attacaaaat cgaaaaatga atggttgggt acaagtttcc aaaacatggt aaagtggact
     3001 ttgcgtatga gacgtaaata gaaaaaaaca cttgttatat gttttctaga attattgttg
     3061 tctctttatg gttggatgat gcaaaatagt aatttcggtt agttgctgta aaacaccacg
     3121 agacaaatag atatggatat ttattaaatc aggaaaaacg taactctcgg ctactggatg
     3181 gttcagtcac ccaacgatta ctggggagag aaaacagggc aaaagcaaag cttaaaggaa
     3241 tccgattgtc attcggcaat gtgcagcgaa actaaaaacc ggataatgga cctgttaatc
     3301 gaaacattga agataaagga agaggaatcc tggcatatca tcaattgaat aagttgaatt
     3361 aattatttca atctcattct cactttctga cttatagtcg ctttgttaaa gctagcggat
     3421 ccatgatgaa ccgtgcgtac tctttaagaa acagccgtgc ccctacggcc tcacaattgt
     3481 taaacccccc tcctccctcc tcttccactc gtagtggtcg tttgtttgcc cccttgagtc
     3541 ataccatgcg tatcaaccac gctgccacct tcacccctga cttggctaag cgtttggctg
     3601 tcttggtcaa gatggaaaag aacgtgatgc gcagtatgga ggtcaccatc cgtggccgtc
     3661 gtgattgtgc tcgccaactt tcctactggg gtgaggactg cgatgacgat atcagcgacg
     3721 tcaccgacaa attgggtgtc ttgttttacg agatggccga gttggagaac tacttgatcg
     3781 atcgttatga ccaataccgt atgacgttga agtccatccg taacatcgag tcctctgtcc
     3841 aaccctctcg tgagaagaag caaaagctct tggatcaaat ctatgctctt aagcataagg
     3901 atcctgaatc ccctcgcttg gtcaccatgg agcaagagct tgttcgtgag gaggctgcct
     3961 gtttggttgc cgaggctcaa ctttccaaca ccacccgtga aaagttcaag caagccatga
     4021 ccttcaactt ggatgccctc cacgagcatg ccgagaagct caacctcatt gccacctatg
     4081 gcagacattt gttgaacttg attgacgaca cccccgtcac tcccggtgag gctcgtcctg
     4141 cctacgatgg ttatgagact tcccgccaaa tcgtcatgga tgctgagcac gccttgagct
     4201 cttgggttcc cagccaaccc gccgtcaact ttgtcaagcc tcaaatcgag gatgatgccc
     4261 aaagtgacgc tcgttcctgg aacgagtatg aggctcaagg tgagcccgtc cccgttgagc
     4321 aagtcaccca cttacaagac gacgttcaat ccgacactag cgagatcatt gagaacgagc
     4381 ctggcatgca ccctcatgtc catgctgagc gtatgtctcg cattgcttct gagtctagcg
     4441 atgccgttcc tcagcaaacc gccgtccagg ttgctggatc catggtgagc aagggcgagg
     4501 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     4561 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     4621 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     4681 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     4741 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     4801 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     4861 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     4921 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     4981 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     5041 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     5101 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     5161 ccaccggcgg catggacgag ctgtacaagg gtagatctat gctgcatgtc aataaatgcc
     5221 aattatgcga aacaagacgt ccttctattt gcgaaagctg tttaaaaaag cagctttatg
     5281 atttttatca taaacgtgat gaattctctc gagatattga gtcagagctt gaaaaggttg
     5341 ctaaattgaa atcagaaagt ttctctctaa aaggaaagat tacccaaaaa gaggaattaa
     5401 cttatcgact acagaacctt gctgcctctc taaacgaaaa gaaatcgtta tcgtgtgact
     5461 tgcattcccg caaacagctt atagaagatg acttgtctaa tcgcaaaaaa tcaattaata
     5521 tcgcgagtca aaagttagct ggcttatcac attcaacgag tgactatttc tctaaggaat
     5581 accttacagc ttatcgccgt tctgagctac ttcaaaataa attgcatgag tatcaaaaga
     5641 agctgataac aagggttttg gatatgtatc aacttcactg gcaaagggat ttagtgctta
     5701 acactcaggg ttgcactcaa aaatctgctc aaatgggatc agagtttaac cctgactcca
     5761 tagattcatc tcttgcgtat agactttcaa aattatcaat gggcaattcg aaatccgact
     5821 tatctcatag tgacaatgaa acaggacatt tttatagcaa ctttttttct caggtgatgg
     5881 agttaaatgc ctctgtgact atttctggca ttcctgtatg tatcagaagt aaagagaaaa
     5941 tgtttttaaa tcctgattgt gctttaactt tatcctttat ttgtatattt ttagctcagt
     6001 ataccagtat tcccttacca tgcccgcttc aactcccttc gcctgatcaa aaacccatga
     6061 cttcattttc atcggaacaa atgttgtata taatgtataa catagtttgg atttcatgga
     6121 actgtggcat tttttcgttt ccccgtggaa ttacccaatc ccagcttttt gagttaatgc
     6181 agtatactgg tttctggtta gttcagctac gtaccaagcc gctaacttct caaaagccag
     6241 attggcatta caagatgggt atggattttg acctttttca ccgattatac ctggcgcgtc
     6301 ttccaattcc aataaattac tcctctcttc gatcacgttc ctcgtctaaa ccatatcagc
     6361 ttatttcatg aagatctggc gcgccacttc taaataagcg aatttcttat gatttatgat
     6421 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     6481 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     6541 tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga
     6601 taacagggta atatactagt aagatgattc ttttatcgtc gataacgatg aggatgtaga
     6661 cgattatgat gagagtgatt agcttctaaa atttggtgtt ttcaaacgct atttacagta
     6721 caatctgaaa aaattggatg aacattgtaa atggtaggat acgagttcca attaatgtat
     6781 tttttaagta ttacacttag cttattaatt ttatatatac attctacctc tgtgggttta
     6841 aagacaactc gtacacatac tcaaattatt tatgaacatt ataatgcata ttcgtactgt
     6901 ctattaaaca tttttttgac attaaaaatt tcgtttacta acgtagaaag cgctgataat
     6961 tgaggctact attttaatat ctagaaacaa agattcatta attaattaag taagctccag
     7021 gatacttgta tatttcgtta aaaaagcgca atttcaacaa ttcctatgaa catccatgat
     7081 ttcatcacaa aatcaaacat atcaggattt aacgtgaatt atttgctttc ttgtgatttt
     7141 catctgattt ctggtcattt acgttactgt aaacaagctt tttaaaaaaa ggatttttta
     7201 cgcaatatta tcgatatccc aatctgtagt ataaaaagtg caacagtgtt gagtttcccg
     7261 aaaccaggca tttttgcggt agggtctgaa actttcacga aaccatttca caacaaaact
     7321 tcttcaacaa catgtgtgca aagaaaatcg tcgtcttacc aggagaccat attggccctg
     7381 aaattgttgc ttctgccttg gaggttttga aagtcgttga gaagaagcga cctgagttaa
     7441 aactcgagtt tgaagaacac aagattggag gtgcctctat tgatgcctat ggaacccctt
     7501 tgactgacga gactgtgaag gcttgtttgg aagctgacgg tgttcttttg ggtgccgttg
     7561 gtggtcctga atggaccaac cccaattgtc gtcctgagca aggtttattg aagcttcgta
     7621 agagtatggg tgtttgggcc aaccttcgac cttgcaactt tgccagcaag tctttagtca
     7681 agtacagccc tttgaagcct gaaatcgttg aaggtgtcga tttttgtgtt gtacgagaac
     7741 ttactggagg ttgttacttt ggtgagcgca ctgaggacaa cggatcgggt tatgctatgg
     7801 acacttggcc ttacagtttg gaagaagttt ctcgtattgc tcgtttggcg gccgcgggcc
     7861 caacaccaat gtttataacc aagttttatc ttgtttgtct acatggtatt ttacattcat
     7921 ctacatacat ctttcattgg ctttgtacat agttatcatt acaagtctaa aaaaattcac
     7981 tcttttctta ttcaatgtca atccaagaga aaagattgtg gtaatgttgt aggagcatgt
     8041 ttaataaatt actatagcaa attacttttt attcccaagg tgtttatcta taatagttaa
     8101 tattttagtc gctacataaa attttaccaa agagtacttg tatactaatt ctaaatgcct
     8161 tctgacataa aacgcctagg aaaacaaacg caaacaaggc atcgactttt tcaataacca
     8221 accaaaaaaa ttttacatta gtcttttttt aatgctgaga aagtctttgc tgatatgcct
     8281 tccaaccagc ttctctatat ctcttggctt cgacaacagg attacgacca gctccataga
     8341 ctccacgacc aacaatgatg atgtcgctac cgcagtttac aatcacttct tcaggagtac
     8401 gatattgctg tcccagcccg tctcctttaa catccaagcc gataccaggg gacatagtta
     8461 tgtagtcgct ttgaaggtta ggaaatcgac gaccagctat aaagccaaag caaaaatcgg
     8521 tatgcttctc aaaccattct aaggttttct ctgtgtagga accagtagcc aaagagcctt
     8581 tggaagacat ttcagccaaa agcaagagac cacgtcccaa aggtaaacca acttctttga
     8641 ggccttgtat aataccctcg cctggcactg tatggcaatt tgtgatatga gcccaagaag
     8701 caattttgta cacaccagat gcatattgta gcttgacggt atttccaatg tctgcgaatt
     8761 tgcgatcctc aaagataaga aaacgatgct ttttacctaa ggccaccagt ttttctacca
     8821 tatcctggtc gaaatcctcg acaacgtcaa tatgtgtctt gataacacag acatagggtc
     8881 caattttatc taccaattct aagatttcgg atttcttcgt caaatcgacc gcgactgaca
     8941 agttgctttg cttttcttcc atcaaagcca acaattcctt ggcaatggga tttttcatcc
     9001 cctcagctct agctgaatag ctttgaaata ctctagcatc cataactttg cttttaaacc
     9061 tttaatttcg atccaagcaa aaaagaggtt cttggtagga caatacggta agaaaacacg
     9121 acatgtgcag agatgccgac gaagcatagt taaactggga tggtaaaatc aattaagaat
     9181 ttataaagac aaaattgtat aagtctctaa aacatcttaa ttatacctca cagaactatc
     9241 taaaatatat tcacaaagtg caaacattat catgaaaaag aaccatttta atttaaagca
     9301 agggcattaa ggcttattta cagaatttct tacttttgta aagattataa ggctgattat
     9361 ctttttcacc atgccaaaaa ttacacaaga tagaatggat gtttgaaatt aaacgtgagt
     9421 atacaaacaa atacactagg taaatcgaaa catttttttc tccattaagt aacaaattcc
     9481 tatttagaga aagaatgctg agtagattaa ataatctata caaacttttt taacacaaat
     9541 gcatacatat agccagtggg atttgtagct aagcttgcga attcgagtct aactccttaa
     9601 ccactcggac atagtgactt atctgacact cattgtaaat taatatatat ataggcattt
     9661 tgtttagtta aaggtactta agtaattagt ataaacgaac caattttata atcaggaagt
     9721 taagtgaatg gtagcacatg tcgtaaaaat tgtgaatttt tattgaataa tattttaaat
     9781 acaagccttt ctagactagg tatactcata aacatatatg agcaaaagga tagaggagat
     9841 tacattgcat cttctacaaa tttatttatt gccctttact gaaaaattaa ataatgagta
     9901 ctaaatgata aaaagcgctc agtacaagaa agattgcaaa aatattgcat tcttcatgaa
     9961 ttaaagttgc atataaggca tattgaaagt aatagtacta aaacagcagt tagcgaaaat
    10021 taatagaatt atattcgcaa gacaattgtg acattaaatt aaaaaattgt agaattttta
    10081 ctatcctctt taacgccatg agcctttata aaaaggttaa attagtttta acattctttt
    10141 tttgagtaag agtttacaat ttatcaaaac ctgtgttatt atattcatta gtttcaattt
    10201 attagcatct agagaaaaat caattggcag ttacattgtt ggaatttatg aagaaaaaga
    10261 atctacaacg gagaataagt tgctgatcgc tttccctaaa attgtatatt ttgctgagct
    10321 tattttgaca tttcgttgaa gttttctcta attcgcattc attttaagta aaacaatgag
    10381 aaataaaatt acaaaaaata caaattaaaa tacaattttt agctataatt atagacgatg
    10441 cccttgtatc ccattctgtc tcgcttgccc ctacttttta tcttttatat accataatga
    10501 acgctgccgc tactaaccat accccgattt tacatttcgg actcccaagg acgtacaaaa
    10561 tagaaaacta tagaaaaaaa taatcagaaa atagcatgtc atctctttgt aaaacgcgtt
    10621 tgcaagaaga aaggaaacaa tggagaagag atcatccatt tgtatgtaaa ttttagtaaa
    10681 cttgaagaaa tcactaacaa cttctcttac ttagggattc tatgcaaaac cttgtaaatc
    10741 atctgatgga ggactcgatt taatgaattg gaaggttggg cccgcggccg cttagctgaa
    10801 acttccaacc ctcctgctcc cgtcacatta ctcgacaaag ctaatgtttt ggcaacttct
    10861 cgtctttggc gcaagactgt agctaagatt tttaaggagg aatatcctca ccttacctta
    10921 aaaaaccagc tcattgactc cgcagccatg cttttggtca agagccctcg tacacttaac
    10981 ggtgttgttt tgactgacaa cttgtttggt gacattattt cagatgaggc ttctgtcatt
    11041 cctggtagct tgggcctttt gccttctgcc tccctttccg gtgtggtagg aaaatcagaa
    11101 gaaaaggttc attgtttggt tgagcccatt cacggtagcg ctcccgagct cgaattcact
    11161 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    11221 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    11281 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
    11341 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
    11401 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    11461 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    11521 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    11581 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
    11641 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    11701 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
    11761 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    11821 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
    11881 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
    11941 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    12001 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cc
//
