LOCUS       sequence-341699-       12598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4948
FEATURES             Location/Qualifiers
     source          1..12598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    94..115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(130..135,136..153,154..160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     173..195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     192..208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     192..208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2492..3199
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2637..2655)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2905..2924
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4954..5141
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5009..5033)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(6805..7599)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      8208..8991
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(9714..9731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9714..9730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9723..9745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9943..10398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10030..10049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10240..10261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10415..10434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10534..10556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10594..10612)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10680..10784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10785..10853,10854..11645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11003..11022)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11816..12404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12305..12324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12558..12575
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt
      241 cgatcgactc tagaggatca gaaaattatc gccataaaag acagaataag tcatcagcgg
      301 ttgtttcatt tcctatattt tttttttatt tttttatttt ttaataaggg aaaatttaac
      361 gtctaaggat acagaagatt gttagcacat taaagtaata aaggcttaag tagtaagtgc
      421 cttagcatgt tattgtattt caaaggacat aatctaaaat aataacaata tcatttctca
      481 caagttattc aattttcttt tttttttcta ataatatcaa gaatgtatta tttgtttgac
      541 ataagtcaac taatttattt aatatgctgg attaatcttg cagacatgta aattaacaag
      601 ttttagtcaa ataacgttga agtttcaatg aactcaaata atttctcttt ttttttatat
      661 aaccataatc tgatttatat tttccgcagg gatcaactga agttatgaca tttggattgg
      721 atcacttata accttggtcg ccaaataata caaaaatcag cgttataaaa caaagaaggt
      781 ttttgttaag aaattaatcc tctttcttga taagaaagtt gaaccgaaat tgcagatact
      841 gatatatgaa aataataccc acaattttgg gaatagcgca agcctcaatt taaacaatag
      901 gtgaggacac atgataatga cctcaatgat tgttagaaga aaagagcctc attacaaaat
      961 cgaaaaatga atggttgggt acaagtttcc aaaacatggt aaagtggact ttgcgtatga
     1021 gacgtaaata gaaaaaaaca cttgttatat gttttctaga attattgttg tctctttatg
     1081 gttggatgat gcaaaatagt aatttcggtt agttgctgta aaacaccacg agacaaatag
     1141 atatggatat ttattaaatc aggaaaaacg taactctcgg ctactggatg gttcagtcac
     1201 ccaacgatta ctggggagag aaaacagggc aaaagcaaag cttaaaggaa tccgattgtc
     1261 attcggcaat gtgcagcgaa actaaaaacc ggataatgga cctgttaatc gaaacattga
     1321 agataaagga agaggaatcc tggcatatca tcaattgaat aagttgaatt aattatttca
     1381 atctcattct cactttctga cttatagtcg ctttgttaaa gctagcggat ccatgatgaa
     1441 ccgtgcgtac tctttaagaa acagccgtgc ccctacggcc tcacaattgt taaacccccc
     1501 tcctccctcc tcttccactc gtagtggtcg tttgtttgcc cccttgagtc ataccatgcg
     1561 tatcaaccac gctgccacct tcacccctga cttggctaag cgtttggctg tcttggtcaa
     1621 gatggaaaag aacgtgatgc gcagtatgga ggtcaccatc cgtggccgtc gtgattgtgc
     1681 tcgccaactt tcctactggg gtgaggactg cgatgacgat atcagcgacg tcaccgacaa
     1741 attgggtgtc ttgttttacg agatggccga gttggagaac tacttgatcg atcgttatga
     1801 ccaataccgt atgacgttga agtccatccg taacatcgag tcctctgtcc aaccctctcg
     1861 tgagaagaag caaaagctct tggatcaaat ctatgctctt aagcataagg atcctgaatc
     1921 ccctcgcttg gtcaccatgg agcaagagct tgttcgtgag gaggctgcct gtttggttgc
     1981 cgaggctcaa ctttccaaca ccacccgtga aaagttcaag caagccatga ccttcaactt
     2041 ggatgccctc cacgagcatg ccgagaagct caacctcatt gccacctatg gcagacattt
     2101 gttgaacttg attgacgaca cccccgtcac tcccggtgag gctcgtcctg cctacgatgg
     2161 ttatgagact tcccgccaaa tcgtcatgga tgctgagcac gccttgagct cttgggttcc
     2221 cagccaaccc gccgtcaact ttgtcaagcc tcaaatcgag gatgatgccc aaagtgacgc
     2281 tcgttcctgg aacgagtatg aggctcaagg tgagcccgtc cccgttgagc aagtcaccca
     2341 cttacaagac gacgttcaat ccgacactag cgagatcatt gagaacgagc ctggcatgca
     2401 ccctcatgtc catgctgagc gtatgtctcg cattgcttct gagtctagcg atgccgttcc
     2461 tcagcaaacc gccgtccagg ttgctggatc catggtgagc aagggcgagg aggataacat
     2521 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     2581 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2641 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     2701 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     2761 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     2821 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     2881 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     2941 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3001 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3061 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3121 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3181 catggacgag ctgtacaagg gtagatctat gtcgcttgaa ttaccaggaa actaccgact
     3241 ttccaggctt aaatcaatac aaataagaaa tgttcaagaa attcaaaatc atagcaaatt
     3301 tgcgatcaat acgacggaaa atttgtggaa acgagatgtg cagttaaaac gtgcaatttc
     3361 cgaaggaact attcgtattt ttatatcatt gcatgtgcag agtaagaaat tgcctgttta
     3421 tattactgaa acgagcggaa atgctgtatg tgaacatgct ttgataaaca aaagtatctg
     3481 aatggtatca ttatatctag gttatttgct tgattgaaaa ggttaatcag tttgtttata
     3541 caagtgattt gccaaaattt cgttgctcaa atccttgaaa caaagccctc aattgatagt
     3601 ttataccttg tatctctttt acccatatac aaattttgta tgtatttatg ttctttgtgt
     3661 ctaacctctc attagaatca catcttttac gtcgatgaaa aagtaaccga aaagcttcaa
     3721 aaataccgtc acgaagagta ctttatcgtg aggacttggt gctcatcaag tagtcatgcc
     3781 ttcaagctac ataaagaatg gaaaattttg agatatgaca gtaattttcg ttatattggt
     3841 aatgatgtac ggttgaatat ttttggttta ccttatgttt cccttgtgtt gactaacata
     3901 ttatttgtag cctgtttttg cagtttgtca tattcgaaac ggtcttttat gtgaatttaa
     3961 tgatggcgtt tacatctaca caacatccca gtcttcggat attatgagac agacctcctt
     4021 tccaaaaagt gcctccacat attctataga caggcggaag gatggttaca ctattcagaa
     4081 aatcactcga attttgaaac tggctgaatg catagatgaa atgcacattg ctaaacatga
     4141 aattcgagct cattttcagg aggaggagtt tcagcagata cgtttgatgc acaaaaggat
     4201 gttacttcgg gatgaaaaaa tcgatgaact ggctaaatta gaacatcttt ggcaaaaaca
     4261 aattaatagc atcactcaga tgaggacgaa atttgacaag acgaaaagct ggttatcctc
     4321 aaaaaggaac actttgaaca aatccaaaga atcccttcaa aaagatgaag ctgagtatgt
     4381 ggaacttgcc aattctttaa aaacaaaagt agagaccaat atagagattc gaatccttat
     4441 ggcacatgct attcgtatgc acgtttccca tctttccaaa atttacccaa tccaaccatc
     4501 gcctggcaat catgacgagt ttacaattcg caatctccgc ctatcttttg agccagataa
     4561 aattaataat gtggaaatgg ctgcttccat aggatttttg gcgcatttac ttcaaactct
     4621 ttcaaagtat ctcgaaaaag aactcgctta tccaattctt tgcgcatctt cgagatcctc
     4681 catcctggat acattgacac ccgacatccc cacgcgtatc tttccccttt atccagcaac
     4741 tcgaccaatt gagctttttg aacatgccat ttatcttctc aaccaggatg taaatgattt
     4801 tttagaaact tttggattac ctattgacca atccatggac atactcagga actttaaaaa
     4861 actgctccaa tttattttat caggacaaca tctatctttt gtacaagtaa cgtctaccgc
     4921 tccttaaaga tctggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
     4981 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     5041 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     5101 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
     5161 agggtaatat actagtaaga tgattctttt atcgtcgata acgatgagga tgtagacgat
     5221 tatgatgaga gtgattagct tctaaaattt ggtgttttca aacgctattt acagtacaat
     5281 ctgaaaaaat tggatgaaca ttgtaaatgg taggatacga gttccaatta atgtattttt
     5341 taagtattac acttagctta ttaattttat atatacattc tacctctgtg ggtttaaaga
     5401 caactcgtac acatactcaa attatttatg aacattataa tgcatattcg tactgtctat
     5461 taaacatttt tttgacatta aaaatttcgt ttactaacgt agaaagcgct gataattgag
     5521 gctactattt taatatctag aaacaaagat tcattaatta attaagtaag ctccaggata
     5581 cttgtatatt tcgttaaaaa agcgcaattt caacaattcc tatgaacatc catgatttca
     5641 tcacaaaatc aaacatatca ggatttaacg tgaattattt gctttcttgt gattttcatc
     5701 tgatttctgg tcatttacgt tactgtaaac aagcttttta aaaaaaggat tttttacgca
     5761 atattatcga tatcccaatc tgtagtataa aaagtgcaac agtgttgagt ttcccgaaac
     5821 caggcatttt tgcggtaggg tctgaaactt tcacgaaacc atttcacaac aaaacttctt
     5881 caacaacatg tgtgcaaaga aaatcgtcgt cttaccagga gaccatattg gccctgaaat
     5941 tgttgcttct gccttggagg ttttgaaagt cgttgagaag aagcgacctg agttaaaact
     6001 cgagtttgaa gaacacaaga ttggaggtgc ctctattgat gcctatggaa cccctttgac
     6061 tgacgagact gtgaaggctt gtttggaagc tgacggtgtt cttttgggtg ccgttggtgg
     6121 tcctgaatgg accaacccca attgtcgtcc tgagcaaggt ttattgaagc ttcgtaagag
     6181 tatgggtgtt tgggccaacc ttcgaccttg caactttgcc agcaagtctt tagtcaagta
     6241 cagccctttg aagcctgaaa tcgttgaagg tgtcgatttt tgtgttgtac gagaacttac
     6301 tggaggttgt tactttggtg agcgcactga ggacaacgga tcgggttatg ctatggacac
     6361 ttggccttac agtttggaag aagtttctcg tattgctcgt ttggcggccg cgggcccaac
     6421 accaatgttt ataaccaagt tttatcttgt ttgtctacat ggtattttac attcatctac
     6481 atacatcttt cattggcttt gtacatagtt atcattacaa gtctaaaaaa attcactctt
     6541 ttcttattca atgtcaatcc aagagaaaag attgtggtaa tgttgtagga gcatgtttaa
     6601 taaattacta tagcaaatta ctttttattc ccaaggtgtt tatctataat agttaatatt
     6661 ttagtcgcta cataaaattt taccaaagag tacttgtata ctaattctaa atgccttctg
     6721 acataaaacg cctaggaaaa caaacgcaaa caaggcatcg actttttcaa taaccaacca
     6781 aaaaaatttt acattagtct ttttttaatg ctgagaaagt ctttgctgat atgccttcca
     6841 accagcttct ctatatctct tggcttcgac aacaggatta cgaccagctc catagactcc
     6901 acgaccaaca atgatgatgt cgctaccgca gtttacaatc acttcttcag gagtacgata
     6961 ttgctgtccc agcccgtctc ctttaacatc caagccgata ccaggggaca tagttatgta
     7021 gtcgctttga aggttaggaa atcgacgacc agctataaag ccaaagcaaa aatcggtatg
     7081 cttctcaaac cattctaagg ttttctctgt gtaggaacca gtagccaaag agcctttgga
     7141 agacatttca gccaaaagca agagaccacg tcccaaaggt aaaccaactt ctttgaggcc
     7201 ttgtataata ccctcgcctg gcactgtatg gcaatttgtg atatgagccc aagaagcaat
     7261 tttgtacaca ccagatgcat attgtagctt gacggtattt ccaatgtctg cgaatttgcg
     7321 atcctcaaag ataagaaaac gatgcttttt acctaaggcc accagttttt ctaccatatc
     7381 ctggtcgaaa tcctcgacaa cgtcaatatg tgtcttgata acacagacat agggtccaat
     7441 tttatctacc aattctaaga tttcggattt cttcgtcaaa tcgaccgcga ctgacaagtt
     7501 gctttgcttt tcttccatca aagccaacaa ttccttggca atgggatttt tcatcccctc
     7561 agctctagct gaatagcttt gaaatactct agcatccata actttgcttt taaaccttta
     7621 atttcgatcc aagcaaaaaa gaggttcttg gtaggacaat acggtaagaa aacacgacat
     7681 gtgcagagat gccgacgaag catagttaaa ctgggatggt aaaatcaatt aagaatttat
     7741 aaagacaaaa ttgtataagt ctctaaaaca tcttaattat acctcacaga actatctaaa
     7801 atatattcac aaagtgcaaa cattatcatg aaaaagaacc attttaattt aaagcaaggg
     7861 cattaaggct tatttacaga atttcttact tttgtaaaga ttataaggct gattatcttt
     7921 ttcaccatgc caaaaattac acaagataga atggatgttt gaaattaaac gtgagtatac
     7981 aaacaaatac actaggtaaa tcgaaacatt tttttctcca ttaagtaaca aattcctatt
     8041 tagagaaaga atgctgagta gattaaataa tctatacaaa cttttttaac acaaatgcat
     8101 acatatagcc agtgggattt gtagctaagc ttgcgaattc gagtctaact ccttaaccac
     8161 tcggacatag tgacttatct gacactcatt gtaaattaat atatatatag gcattttgtt
     8221 tagttaaagg tacttaagta attagtataa acgaaccaat tttataatca ggaagttaag
     8281 tgaatggtag cacatgtcgt aaaaattgtg aatttttatt gaataatatt ttaaatacaa
     8341 gcctttctag actaggtata ctcataaaca tatatgagca aaaggataga ggagattaca
     8401 ttgcatcttc tacaaattta tttattgccc tttactgaaa aattaaataa tgagtactaa
     8461 atgataaaaa gcgctcagta caagaaagat tgcaaaaata ttgcattctt catgaattaa
     8521 agttgcatat aaggcatatt gaaagtaata gtactaaaac agcagttagc gaaaattaat
     8581 agaattatat tcgcaagaca attgtgacat taaattaaaa aattgtagaa tttttactat
     8641 cctctttaac gccatgagcc tttataaaaa ggttaaatta gttttaacat tctttttttg
     8701 agtaagagtt tacaatttat caaaacctgt gttattatat tcattagttt caatttatta
     8761 gcatctagag aaaaatcaat tggcagttac attgttggaa tttatgaaga aaaagaatct
     8821 acaacggaga ataagttgct gatcgctttc cctaaaattg tatattttgc tgagcttatt
     8881 ttgacatttc gttgaagttt tctctaattc gcattcattt taagtaaaac aatgagaaat
     8941 aaaattacaa aaaatacaaa ttaaaataca atttttagct ataattatag acgatgccct
     9001 tgtatcccat tctgtctcgc ttgcccctac tttttatctt ttatatacca taatgaacgc
     9061 tgccgctact aaccataccc cgattttaca tttcggactc ccaaggacgt acaaaataga
     9121 aaactataga aaaaaataat cagaaaatag catgtcatct ctttgtaaaa cgcgtttgca
     9181 agaagaaagg aaacaatgga gaagagatca tccatttgta tgtaaatttt agtaaacttg
     9241 aagaaatcac taacaacttc tcttacttag ggattctatg caaaaccttg taaatcatct
     9301 gatggaggac tcgatttaat gaattggaag gttgggcccg cggccgctta gctgaaactt
     9361 ccaaccctcc tgctcccgtc acattactcg acaaagctaa tgttttggca acttctcgtc
     9421 tttggcgcaa gactgtagct aagattttta aggaggaata tcctcacctt accttaaaaa
     9481 accagctcat tgactccgca gccatgcttt tggtcaagag ccctcgtaca cttaacggtg
     9541 ttgttttgac tgacaacttg tttggtgaca ttatttcaga tgaggcttct gtcattcctg
     9601 gtagcttggg ccttttgcct tctgcctccc tttccggtgt ggtaggaaaa tcagaagaaa
     9661 aggttcattg tttggttgag cccattcacg gtagcgctcc cgagctcgaa ttcactggcc
     9721 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9781 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9841 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     9901 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     9961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
    10021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10141 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10261 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
    10321 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    10381 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10441 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    10501 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    10561 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
    10621 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10681 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10741 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10801 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10861 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10921 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10981 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11041 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11101 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11161 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11221 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11281 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11341 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11401 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11461 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11521 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11581 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11641 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11701 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12481 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12541 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacg
//
