LOCUS       sequence-341700-       11218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4953
FEATURES             Location/Qualifiers
     source          1..11218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..30
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    147..168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(183..188,189..206,207..213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     226..248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     245..261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1486..2199
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      3627..3814
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3682..3706)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5478..6272)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      6881..7664
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(8387..8404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8387..8403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8396..8418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8616..9071
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8703..8722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8913..8934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9088..9107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9267..9285)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9353..9457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9458..9526,9527..10318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9676..9695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10489..11077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10978..10997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
       61 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgatcga
      301 ctctagagga tcagaaaatt atcgccataa aagacagaat aagtcatcag cggttgtttc
      361 atttcctata tttttttttt atttttttat tttttaataa gggaaaattt aacgtctaag
      421 gatacagaag attgttagca cattaaagta ataaaggctt aagtagtaag tgccttagca
      481 tgttattgta tttcaaagga cataatctaa aataataaca atatcatttc tcacaagtta
      541 ttcaattttc tttttttttt ctaataatat caagaatgta ttatttgttt gacataagtc
      601 aactaattta tttaatatgc tggattaatc ttgcagacat gtaaattaac aagttttagt
      661 caaataacgt tgaagtttca atgaactcaa ataatttctc ttttttttta tataaccata
      721 atctgattta tattttccgc agggatcaac tgaagttatg acatttggat tggatcactt
      781 ataaccttgg tcgccaaata atacaaaaat cagcgttata aaacaaagaa ggtttttgtt
      841 aagaaattaa tcctctttct tgataagaaa gttgaaccga aattgcagat actgatatat
      901 gaaaataata cccacaattt tgggaatagc gcaagcctca atttaaacaa taggtgagga
      961 cacatgataa tgacctcaat gattgttaga agaaaagagc ctcattacaa aatcgaaaaa
     1021 tgaatggttg ggtacaagtt tccaaaacat ggtaaagtgg actttgcgta tgagacgtaa
     1081 atagaaaaaa acacttgtta tatgttttct agaattattg ttgtctcttt atggttggat
     1141 gatgcaaaat agtaatttcg gttagttgct gtaaaacacc acgagacaaa tagatatgga
     1201 tatttattaa atcaggaaaa acgtaactct cggctactgg atggttcagt cacccaacga
     1261 ttactgggga gagaaaacag ggcaaaagca aagcttaaag gaatccgatt gtcattcggc
     1321 aatgtgcagc gaaactaaaa accggataat ggacctgtta atcgaaacat tgaagataaa
     1381 ggaagaggaa tcctggcata tcatcaattg aataagttga attaattatt tcaatctcat
     1441 tctcactttc tgacttatag tcgctttgtt aaagctagcg gatccatgtc taaaggtgaa
     1501 gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac
     1561 aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa
     1621 tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcggt
     1681 tatggtgttc aatgttttgc gagataccca gatcatatga aacaacatga ctttttcaag
     1741 tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac
     1801 tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta
     1861 aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga atacaactat
     1921 aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc
     1981 aaaattagac acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat
     2041 actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct
     2101 gccttatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact
     2161 gctgctggta ttacccatgg tatggatgaa ttgtacaaaa gatctatgca gtatttgtgt
     2221 cagaggtgtc attctttgat aaattttaaa gatgtttacg atgatgactt gttaaagcta
     2281 aaaaatcttc cgaaaagtag atttgttcaa gcatcatcgt tgacagagat gaatgagagt
     2341 ggggagagtg atgatcaaat gaattcttct tctgaagact atccagctca gagattgcaa
     2401 ctttacaaaa aaacaataag cgaaggtgat tacaattttg ataatgtacc accgcccgaa
     2461 ttgaggacgc ccactttaga ttcatttgtt gtattgccag ctgctaaaga tggctatgaa
     2521 gaagaaaaga attcgcctga agaggtgaat gatcttttta gttggaaaat agaaatttat
     2581 aacagaatat ttgatctttt atcctctaaa accaaagtcg atcaccctct ttgtgtcgaa
     2641 tgtgctgagt tattgactga agagatgtct aaaacattaa gggccctaaa agaggaaaaa
     2701 aaaatgtatt ttaattatga taatttctta tcaagtcaaa cagtgcatga agaaaatact
     2761 gctgcgcttg attctgagat agatgaatta atgaaacaga ttaatgagaa agaagaaaag
     2821 atcgaggaaa ttagtgatga aacagacaaa cttcaaaagt tgctacgtga attggacgaa
     2881 gaaaaggaaa aagtttatgc cgaggagcaa gagttctaca ataatctaaa ccaatttcaa
     2941 ataaaaaagc tgagtttaga aagacaatac gattgtgcaa acttggagtt tgagcataac
     3001 tctaggaagc tcgagaagct tcagaaaatg aatgttttca gtgatatttt ttacatttct
     3061 cattattctg agccgaatgg agagggctct attgctacaa ttaatggctt acgcttggga
     3121 aggcttccaa gccagaaagt taattgggct gagataaatg ccgcttgggg gatgactgtt
     3181 ttactattag atgttttgac tgaaaagtta gactttcatt cctcttccta tcagctgaag
     3241 ccttttggca gccaatcttt tataattcgt tttgatcgcg atcctaatgg aaatcaagtt
     3301 aaaccaacaa agctagatct tttttctagc ggagagctca aaatatttat gaatcgtaga
     3361 tttgaccaag gcatggtggc ttttctggat tacttacatc aatttggcga tttttgtgct
     3421 gctaaaacac cttcagcggt tttaccctat gccattgaaa atgaccgtat cggcggaaaa
     3481 tgcatacgtt tagcattcaa tcaggacgag aattggactc gtgcgttgaa gttcgtccta
     3541 acggatatca aatttttgga agcttacgtt tcttctcagg acaagcagtc gaacttttaa
     3601 agatctggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     3661 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     3721 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     3781 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
     3841 tatactagta agatgattct tttatcgtcg ataacgatga ggatgtagac gattatgatg
     3901 agagtgatta gcttctaaaa tttggtgttt tcaaacgcta tttacagtac aatctgaaaa
     3961 aattggatga acattgtaaa tggtaggata cgagttccaa ttaatgtatt ttttaagtat
     4021 tacacttagc ttattaattt tatatataca ttctacctct gtgggtttaa agacaactcg
     4081 tacacatact caaattattt atgaacatta taatgcatat tcgtactgtc tattaaacat
     4141 ttttttgaca ttaaaaattt cgtttactaa cgtagaaagc gctgataatt gaggctacta
     4201 ttttaatatc tagaaacaaa gattcattaa ttaattaagt aagctccagg atacttgtat
     4261 atttcgttaa aaaagcgcaa tttcaacaat tcctatgaac atccatgatt tcatcacaaa
     4321 atcaaacata tcaggattta acgtgaatta tttgctttct tgtgattttc atctgatttc
     4381 tggtcattta cgttactgta aacaagcttt ttaaaaaaag gattttttac gcaatattat
     4441 cgatatccca atctgtagta taaaaagtgc aacagtgttg agtttcccga aaccaggcat
     4501 ttttgcggta gggtctgaaa ctttcacgaa accatttcac aacaaaactt cttcaacaac
     4561 atgtgtgcaa agaaaatcgt cgtcttacca ggagaccata ttggccctga aattgttgct
     4621 tctgccttgg aggttttgaa agtcgttgag aagaagcgac ctgagttaaa actcgagttt
     4681 gaagaacaca agattggagg tgcctctatt gatgcctatg gaaccccttt gactgacgag
     4741 actgtgaagg cttgtttgga agctgacggt gttcttttgg gtgccgttgg tggtcctgaa
     4801 tggaccaacc ccaattgtcg tcctgagcaa ggtttattga agcttcgtaa gagtatgggt
     4861 gtttgggcca accttcgacc ttgcaacttt gccagcaagt ctttagtcaa gtacagccct
     4921 ttgaagcctg aaatcgttga aggtgtcgat ttttgtgttg tacgagaact tactggaggt
     4981 tgttactttg gtgagcgcac tgaggacaac ggatcgggtt atgctatgga cacttggcct
     5041 tacagtttgg aagaagtttc tcgtattgct cgtttggcgg ccgcgggccc aacaccaatg
     5101 tttataacca agttttatct tgtttgtcta catggtattt tacattcatc tacatacatc
     5161 tttcattggc tttgtacata gttatcatta caagtctaaa aaaattcact cttttcttat
     5221 tcaatgtcaa tccaagagaa aagattgtgg taatgttgta ggagcatgtt taataaatta
     5281 ctatagcaaa ttacttttta ttcccaaggt gtttatctat aatagttaat attttagtcg
     5341 ctacataaaa ttttaccaaa gagtacttgt atactaattc taaatgcctt ctgacataaa
     5401 acgcctagga aaacaaacgc aaacaaggca tcgacttttt caataaccaa ccaaaaaaat
     5461 tttacattag tcttttttta atgctgagaa agtctttgct gatatgcctt ccaaccagct
     5521 tctctatatc tcttggcttc gacaacagga ttacgaccag ctccatagac tccacgacca
     5581 acaatgatga tgtcgctacc gcagtttaca atcacttctt caggagtacg atattgctgt
     5641 cccagcccgt ctcctttaac atccaagccg ataccagggg acatagttat gtagtcgctt
     5701 tgaaggttag gaaatcgacg accagctata aagccaaagc aaaaatcggt atgcttctca
     5761 aaccattcta aggttttctc tgtgtaggaa ccagtagcca aagagccttt ggaagacatt
     5821 tcagccaaaa gcaagagacc acgtcccaaa ggtaaaccaa cttctttgag gccttgtata
     5881 ataccctcgc ctggcactgt atggcaattt gtgatatgag cccaagaagc aattttgtac
     5941 acaccagatg catattgtag cttgacggta tttccaatgt ctgcgaattt gcgatcctca
     6001 aagataagaa aacgatgctt tttacctaag gccaccagtt tttctaccat atcctggtcg
     6061 aaatcctcga caacgtcaat atgtgtcttg ataacacaga catagggtcc aattttatct
     6121 accaattcta agatttcgga tttcttcgtc aaatcgaccg cgactgacaa gttgctttgc
     6181 ttttcttcca tcaaagccaa caattccttg gcaatgggat ttttcatccc ctcagctcta
     6241 gctgaatagc tttgaaatac tctagcatcc ataactttgc ttttaaacct ttaatttcga
     6301 tccaagcaaa aaagaggttc ttggtaggac aatacggtaa gaaaacacga catgtgcaga
     6361 gatgccgacg aagcatagtt aaactgggat ggtaaaatca attaagaatt tataaagaca
     6421 aaattgtata agtctctaaa acatcttaat tatacctcac agaactatct aaaatatatt
     6481 cacaaagtgc aaacattatc atgaaaaaga accattttaa tttaaagcaa gggcattaag
     6541 gcttatttac agaatttctt acttttgtaa agattataag gctgattatc tttttcacca
     6601 tgccaaaaat tacacaagat agaatggatg tttgaaatta aacgtgagta tacaaacaaa
     6661 tacactaggt aaatcgaaac atttttttct ccattaagta acaaattcct atttagagaa
     6721 agaatgctga gtagattaaa taatctatac aaactttttt aacacaaatg catacatata
     6781 gccagtggga tttgtagcta agcttgcgaa ttcgagtcta actccttaac cactcggaca
     6841 tagtgactta tctgacactc attgtaaatt aatatatata taggcatttt gtttagttaa
     6901 aggtacttaa gtaattagta taaacgaacc aattttataa tcaggaagtt aagtgaatgg
     6961 tagcacatgt cgtaaaaatt gtgaattttt attgaataat attttaaata caagcctttc
     7021 tagactaggt atactcataa acatatatga gcaaaaggat agaggagatt acattgcatc
     7081 ttctacaaat ttatttattg ccctttactg aaaaattaaa taatgagtac taaatgataa
     7141 aaagcgctca gtacaagaaa gattgcaaaa atattgcatt cttcatgaat taaagttgca
     7201 tataaggcat attgaaagta atagtactaa aacagcagtt agcgaaaatt aatagaatta
     7261 tattcgcaag acaattgtga cattaaatta aaaaattgta gaatttttac tatcctcttt
     7321 aacgccatga gcctttataa aaaggttaaa ttagttttaa cattcttttt ttgagtaaga
     7381 gtttacaatt tatcaaaacc tgtgttatta tattcattag tttcaattta ttagcatcta
     7441 gagaaaaatc aattggcagt tacattgttg gaatttatga agaaaaagaa tctacaacgg
     7501 agaataagtt gctgatcgct ttccctaaaa ttgtatattt tgctgagctt attttgacat
     7561 ttcgttgaag ttttctctaa ttcgcattca ttttaagtaa aacaatgaga aataaaatta
     7621 caaaaaatac aaattaaaat acaattttta gctataatta tagacgatgc ccttgtatcc
     7681 cattctgtct cgcttgcccc tactttttat cttttatata ccataatgaa cgctgccgct
     7741 actaaccata ccccgatttt acatttcgga ctcccaagga cgtacaaaat agaaaactat
     7801 agaaaaaaat aatcagaaaa tagcatgtca tctctttgta aaacgcgttt gcaagaagaa
     7861 aggaaacaat ggagaagaga tcatccattt gtatgtaaat tttagtaaac ttgaagaaat
     7921 cactaacaac ttctcttact tagggattct atgcaaaacc ttgtaaatca tctgatggag
     7981 gactcgattt aatgaattgg aaggttgggc ccgcggccgc ttagctgaaa cttccaaccc
     8041 tcctgctccc gtcacattac tcgacaaagc taatgttttg gcaacttctc gtctttggcg
     8101 caagactgta gctaagattt ttaaggagga atatcctcac cttaccttaa aaaaccagct
     8161 cattgactcc gcagccatgc ttttggtcaa gagccctcgt acacttaacg gtgttgtttt
     8221 gactgacaac ttgtttggtg acattatttc agatgaggct tctgtcattc ctggtagctt
     8281 gggccttttg ccttctgcct ccctttccgg tgtggtagga aaatcagaag aaaaggttca
     8341 ttgtttggtt gagcccattc acggtagcgc tcccgagctc gaattcactg gccgtcgttt
     8401 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8461 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8521 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     8581 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     8641 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     8701 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8761 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8821 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8881 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     8941 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     9001 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9061 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9121 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     9181 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     9241 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     9301 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     9361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    10501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    10561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    10621 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    10681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    10741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    10801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    10861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    10921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    10981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11161 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccga
//
