LOCUS       sequence-341701-       10873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4924
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2493..3200
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2638..2656)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2906..2925
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3230..3417
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3285..3309)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5081..5875)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      6484..7267
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7990..8007)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7990..8006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7999..8021)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8219..8674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8306..8325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8516..8537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8691..8710)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8810..8832
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8870..8888)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8956..9060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9061..9129,9130..9921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9279..9298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10092..10680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10581..10600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10834..10851
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
      241 tcgatcgact ctagaggatc agaaaattat cgccataaaa gacagaataa gtcatcagcg
      301 gttgtttcat ttcctatatt ttttttttat ttttttattt tttaataagg gaaaatttaa
      361 cgtctaagga tacagaagat tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg
      421 ccttagcatg ttattgtatt tcaaaggaca taatctaaaa taataacaat atcatttctc
      481 acaagttatt caattttctt ttttttttct aataatatca agaatgtatt atttgtttga
      541 cataagtcaa ctaatttatt taatatgctg gattaatctt gcagacatgt aaattaacaa
      601 gttttagtca aataacgttg aagtttcaat gaactcaaat aatttctctt tttttttata
      661 taaccataat ctgatttata ttttccgcag ggatcaactg aagttatgac atttggattg
      721 gatcacttat aaccttggtc gccaaataat acaaaaatca gcgttataaa acaaagaagg
      781 tttttgttaa gaaattaatc ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac
      841 tgatatatga aaataatacc cacaattttg ggaatagcgc aagcctcaat ttaaacaata
      901 ggtgaggaca catgataatg acctcaatga ttgttagaag aaaagagcct cattacaaaa
      961 tcgaaaaatg aatggttggg tacaagtttc caaaacatgg taaagtggac tttgcgtatg
     1021 agacgtaaat agaaaaaaac acttgttata tgttttctag aattattgtt gtctctttat
     1081 ggttggatga tgcaaaatag taatttcggt tagttgctgt aaaacaccac gagacaaata
     1141 gatatggata tttattaaat caggaaaaac gtaactctcg gctactggat ggttcagtca
     1201 cccaacgatt actggggaga gaaaacaggg caaaagcaaa gcttaaagga atccgattgt
     1261 cattcggcaa tgtgcagcga aactaaaaac cggataatgg acctgttaat cgaaacattg
     1321 aagataaagg aagaggaatc ctggcatatc atcaattgaa taagttgaat taattatttc
     1381 aatctcattc tcactttctg acttatagtc gctttgttaa agctagcgga tccatgatga
     1441 accgtgcgta ctctttaaga aacagccgtg cccctacggc ctcacaattg ttaaaccccc
     1501 ctcctccctc ctcttccact cgtagtggtc gtttgtttgc ccccttgagt cataccatgc
     1561 gtatcaacca cgctgccacc ttcacccctg acttggctaa gcgtttggct gtcttggtca
     1621 agatggaaaa gaacgtgatg cgcagtatgg aggtcaccat ccgtggccgt cgtgattgtg
     1681 ctcgccaact ttcctactgg ggtgaggact gcgatgacga tatcagcgac gtcaccgaca
     1741 aattgggtgt cttgttttac gagatggccg agttggagaa ctacttgatc gatcgttatg
     1801 accaataccg tatgacgttg aagtccatcc gtaacatcga gtcctctgtc caaccctctc
     1861 gtgagaagaa gcaaaagctc ttggatcaaa tctatgctct taagcataag gatcctgaat
     1921 cccctcgctt ggtcaccatg gagcaagagc ttgttcgtga ggaggctgcc tgtttggttg
     1981 ccgaggctca actttccaac accacccgtg aaaagttcaa gcaagccatg accttcaact
     2041 tggatgccct ccacgagcat gccgagaagc tcaacctcat tgccacctat ggcagacatt
     2101 tgttgaactt gattgacgac acccccgtca ctcccggtga ggctcgtcct gcctacgatg
     2161 gttatgagac ttcccgccaa atcgtcatgg atgctgagca cgccttgagc tcttgggttc
     2221 ccagccaacc cgccgtcaac tttgtcaagc ctcaaatcga ggatgatgcc caaagtgacg
     2281 ctcgttcctg gaacgagtat gaggctcaag gtgagcccgt ccccgttgag caagtcaccc
     2341 acttacaaga cgacgttcaa tccgacacta gcgagatcat tgagaacgag cctggcatgc
     2401 accctcatgt ccatgctgag cgtatgtctc gcattgcttc tgagtctagc gatgccgttc
     2461 ctcagcaaac cgccgtccag gttgctggat ccatggtgag caagggcgag gaggataaca
     2521 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     2581 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     2641 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     2701 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     2761 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2821 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     2881 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2941 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     3001 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     3061 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     3121 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     3181 gcatggacga gctgtacaag ggtagatctg gcgcgccact tctaaataag cgaatttctt
     3241 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     3301 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     3361 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
     3421 atccgctagg gataacaggg taatatacta gtaagatgat tcttttatcg tcgataacga
     3481 tgaggatgta gacgattatg atgagagtga ttagcttcta aaatttggtg ttttcaaacg
     3541 ctatttacag tacaatctga aaaaattgga tgaacattgt aaatggtagg atacgagttc
     3601 caattaatgt attttttaag tattacactt agcttattaa ttttatatat acattctacc
     3661 tctgtgggtt taaagacaac tcgtacacat actcaaatta tttatgaaca ttataatgca
     3721 tattcgtact gtctattaaa catttttttg acattaaaaa tttcgtttac taacgtagaa
     3781 agcgctgata attgaggcta ctattttaat atctagaaac aaagattcat taattaatta
     3841 agtaagctcc aggatacttg tatatttcgt taaaaaagcg caatttcaac aattcctatg
     3901 aacatccatg atttcatcac aaaatcaaac atatcaggat ttaacgtgaa ttatttgctt
     3961 tcttgtgatt ttcatctgat ttctggtcat ttacgttact gtaaacaagc tttttaaaaa
     4021 aaggattttt tacgcaatat tatcgatatc ccaatctgta gtataaaaag tgcaacagtg
     4081 ttgagtttcc cgaaaccagg catttttgcg gtagggtctg aaactttcac gaaaccattt
     4141 cacaacaaaa cttcttcaac aacatgtgtg caaagaaaat cgtcgtctta ccaggagacc
     4201 atattggccc tgaaattgtt gcttctgcct tggaggtttt gaaagtcgtt gagaagaagc
     4261 gacctgagtt aaaactcgag tttgaagaac acaagattgg aggtgcctct attgatgcct
     4321 atggaacccc tttgactgac gagactgtga aggcttgttt ggaagctgac ggtgttcttt
     4381 tgggtgccgt tggtggtcct gaatggacca accccaattg tcgtcctgag caaggtttat
     4441 tgaagcttcg taagagtatg ggtgtttggg ccaaccttcg accttgcaac tttgccagca
     4501 agtctttagt caagtacagc cctttgaagc ctgaaatcgt tgaaggtgtc gatttttgtg
     4561 ttgtacgaga acttactgga ggttgttact ttggtgagcg cactgaggac aacggatcgg
     4621 gttatgctat ggacacttgg ccttacagtt tggaagaagt ttctcgtatt gctcgtttgg
     4681 cggccgcggg cccaacacca atgtttataa ccaagtttta tcttgtttgt ctacatggta
     4741 ttttacattc atctacatac atctttcatt ggctttgtac atagttatca ttacaagtct
     4801 aaaaaaattc actcttttct tattcaatgt caatccaaga gaaaagattg tggtaatgtt
     4861 gtaggagcat gtttaataaa ttactatagc aaattacttt ttattcccaa ggtgtttatc
     4921 tataatagtt aatattttag tcgctacata aaattttacc aaagagtact tgtatactaa
     4981 ttctaaatgc cttctgacat aaaacgccta ggaaaacaaa cgcaaacaag gcatcgactt
     5041 tttcaataac caaccaaaaa aattttacat tagtcttttt ttaatgctga gaaagtcttt
     5101 gctgatatgc cttccaacca gcttctctat atctcttggc ttcgacaaca ggattacgac
     5161 cagctccata gactccacga ccaacaatga tgatgtcgct accgcagttt acaatcactt
     5221 cttcaggagt acgatattgc tgtcccagcc cgtctccttt aacatccaag ccgataccag
     5281 gggacatagt tatgtagtcg ctttgaaggt taggaaatcg acgaccagct ataaagccaa
     5341 agcaaaaatc ggtatgcttc tcaaaccatt ctaaggtttt ctctgtgtag gaaccagtag
     5401 ccaaagagcc tttggaagac atttcagcca aaagcaagag accacgtccc aaaggtaaac
     5461 caacttcttt gaggccttgt ataataccct cgcctggcac tgtatggcaa tttgtgatat
     5521 gagcccaaga agcaattttg tacacaccag atgcatattg tagcttgacg gtatttccaa
     5581 tgtctgcgaa tttgcgatcc tcaaagataa gaaaacgatg ctttttacct aaggccacca
     5641 gtttttctac catatcctgg tcgaaatcct cgacaacgtc aatatgtgtc ttgataacac
     5701 agacataggg tccaatttta tctaccaatt ctaagatttc ggatttcttc gtcaaatcga
     5761 ccgcgactga caagttgctt tgcttttctt ccatcaaagc caacaattcc ttggcaatgg
     5821 gatttttcat cccctcagct ctagctgaat agctttgaaa tactctagca tccataactt
     5881 tgcttttaaa cctttaattt cgatccaagc aaaaaagagg ttcttggtag gacaatacgg
     5941 taagaaaaca cgacatgtgc agagatgccg acgaagcata gttaaactgg gatggtaaaa
     6001 tcaattaaga atttataaag acaaaattgt ataagtctct aaaacatctt aattatacct
     6061 cacagaacta tctaaaatat attcacaaag tgcaaacatt atcatgaaaa agaaccattt
     6121 taatttaaag caagggcatt aaggcttatt tacagaattt cttacttttg taaagattat
     6181 aaggctgatt atctttttca ccatgccaaa aattacacaa gatagaatgg atgtttgaaa
     6241 ttaaacgtga gtatacaaac aaatacacta ggtaaatcga aacatttttt tctccattaa
     6301 gtaacaaatt cctatttaga gaaagaatgc tgagtagatt aaataatcta tacaaacttt
     6361 tttaacacaa atgcatacat atagccagtg ggatttgtag ctaagcttgc gaattcgagt
     6421 ctaactcctt aaccactcgg acatagtgac ttatctgaca ctcattgtaa attaatatat
     6481 atataggcat tttgtttagt taaaggtact taagtaatta gtataaacga accaatttta
     6541 taatcaggaa gttaagtgaa tggtagcaca tgtcgtaaaa attgtgaatt tttattgaat
     6601 aatattttaa atacaagcct ttctagacta ggtatactca taaacatata tgagcaaaag
     6661 gatagaggag attacattgc atcttctaca aatttattta ttgcccttta ctgaaaaatt
     6721 aaataatgag tactaaatga taaaaagcgc tcagtacaag aaagattgca aaaatattgc
     6781 attcttcatg aattaaagtt gcatataagg catattgaaa gtaatagtac taaaacagca
     6841 gttagcgaaa attaatagaa ttatattcgc aagacaattg tgacattaaa ttaaaaaatt
     6901 gtagaatttt tactatcctc tttaacgcca tgagccttta taaaaaggtt aaattagttt
     6961 taacattctt tttttgagta agagtttaca atttatcaaa acctgtgtta ttatattcat
     7021 tagtttcaat ttattagcat ctagagaaaa atcaattggc agttacattg ttggaattta
     7081 tgaagaaaaa gaatctacaa cggagaataa gttgctgatc gctttcccta aaattgtata
     7141 ttttgctgag cttattttga catttcgttg aagttttctc taattcgcat tcattttaag
     7201 taaaacaatg agaaataaaa ttacaaaaaa tacaaattaa aatacaattt ttagctataa
     7261 ttatagacga tgcccttgta tcccattctg tctcgcttgc ccctactttt tatcttttat
     7321 ataccataat gaacgctgcc gctactaacc ataccccgat tttacatttc ggactcccaa
     7381 ggacgtacaa aatagaaaac tatagaaaaa aataatcaga aaatagcatg tcatctcttt
     7441 gtaaaacgcg tttgcaagaa gaaaggaaac aatggagaag agatcatcca tttgtatgta
     7501 aattttagta aacttgaaga aatcactaac aacttctctt acttagggat tctatgcaaa
     7561 accttgtaaa tcatctgatg gaggactcga tttaatgaat tggaaggttg ggcccgcggc
     7621 cgcttagctg aaacttccaa ccctcctgct cccgtcacat tactcgacaa agctaatgtt
     7681 ttggcaactt ctcgtctttg gcgcaagact gtagctaaga tttttaagga ggaatatcct
     7741 caccttacct taaaaaacca gctcattgac tccgcagcca tgcttttggt caagagccct
     7801 cgtacactta acggtgttgt tttgactgac aacttgtttg gtgacattat ttcagatgag
     7861 gcttctgtca ttcctggtag cttgggcctt ttgccttctg cctccctttc cggtgtggta
     7921 ggaaaatcag aagaaaaggt tcattgtttg gttgagccca ttcacggtag cgctcccgag
     7981 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8041 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8101 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     8161 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     8221 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8281 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8341 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8401 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     8461 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8521 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     8581 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8641 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     8701 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     8761 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     8821 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     8881 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9601 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9661 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9721 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9781 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9901 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10141 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10741 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10801 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10861 gagcgcccaa tac
//
