LOCUS       sequence-341702-        9817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDUAL plasmid expressing GFP from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB1751
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    54..75
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(90..95,96..113,114..120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    128..144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     133..155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     152..168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     152..168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1393..2106
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2133..2320
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2188..2212)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3984..4778)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      5387..6170
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(6893..6910)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6893..6909)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6902..6924)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7122..7577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7209..7228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7419..7440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7594..7613)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7713..7735
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7773..7791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7859..7963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7964..8032,8033..8824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8182..8201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8995..9583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9484..9503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9737..9754
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
       61 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      121 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      181 aagcttgcat gcctgcaggt cgatcgactc tagaggatca gaaaattatc gccataaaag
      241 acagaataag tcatcagcgg ttgtttcatt tcctatattt tttttttatt tttttatttt
      301 ttaataaggg aaaatttaac gtctaaggat acagaagatt gttagcacat taaagtaata
      361 aaggcttaag tagtaagtgc cttagcatgt tattgtattt caaaggacat aatctaaaat
      421 aataacaata tcatttctca caagttattc aattttcttt tttttttcta ataatatcaa
      481 gaatgtatta tttgtttgac ataagtcaac taatttattt aatatgctgg attaatcttg
      541 cagacatgta aattaacaag ttttagtcaa ataacgttga agtttcaatg aactcaaata
      601 atttctcttt ttttttatat aaccataatc tgatttatat tttccgcagg gatcaactga
      661 agttatgaca tttggattgg atcacttata accttggtcg ccaaataata caaaaatcag
      721 cgttataaaa caaagaaggt ttttgttaag aaattaatcc tctttcttga taagaaagtt
      781 gaaccgaaat tgcagatact gatatatgaa aataataccc acaattttgg gaatagcgca
      841 agcctcaatt taaacaatag gtgaggacac atgataatga cctcaatgat tgttagaaga
      901 aaagagcctc attacaaaat cgaaaaatga atggttgggt acaagtttcc aaaacatggt
      961 aaagtggact ttgcgtatga gacgtaaata gaaaaaaaca cttgttatat gttttctaga
     1021 attattgttg tctctttatg gttggatgat gcaaaatagt aatttcggtt agttgctgta
     1081 aaacaccacg agacaaatag atatggatat ttattaaatc aggaaaaacg taactctcgg
     1141 ctactggatg gttcagtcac ccaacgatta ctggggagag aaaacagggc aaaagcaaag
     1201 cttaaaggaa tccgattgtc attcggcaat gtgcagcgaa actaaaaacc ggataatgga
     1261 cctgttaatc gaaacattga agataaagga agaggaatcc tggcatatca tcaattgaat
     1321 aagttgaatt aattatttca atctcattct cactttctga cttatagtcg ctttgttaaa
     1381 gctagcggat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     1441 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     1501 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     1561 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     1621 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     1681 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     1741 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     1801 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     1861 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     1921 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     1981 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     2041 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     2101 tacaaaagat ctggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     2161 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2221 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2281 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca
     2341 gggtaatata ctagtaagat gattctttta tcgtcgataa cgatgaggat gtagacgatt
     2401 atgatgagag tgattagctt ctaaaatttg gtgttttcaa acgctattta cagtacaatc
     2461 tgaaaaaatt ggatgaacat tgtaaatggt aggatacgag ttccaattaa tgtatttttt
     2521 aagtattaca cttagcttat taattttata tatacattct acctctgtgg gtttaaagac
     2581 aactcgtaca catactcaaa ttatttatga acattataat gcatattcgt actgtctatt
     2641 aaacattttt ttgacattaa aaatttcgtt tactaacgta gaaagcgctg ataattgagg
     2701 ctactatttt aatatctaga aacaaagatt cattaattaa ttaagtaagc tccaggatac
     2761 ttgtatattt cgttaaaaaa gcgcaatttc aacaattcct atgaacatcc atgatttcat
     2821 cacaaaatca aacatatcag gatttaacgt gaattatttg ctttcttgtg attttcatct
     2881 gatttctggt catttacgtt actgtaaaca agctttttaa aaaaaggatt ttttacgcaa
     2941 tattatcgat atcccaatct gtagtataaa aagtgcaaca gtgttgagtt tcccgaaacc
     3001 aggcattttt gcggtagggt ctgaaacttt cacgaaacca tttcacaaca aaacttcttc
     3061 aacaacatgt gtgcaaagaa aatcgtcgtc ttaccaggag accatattgg ccctgaaatt
     3121 gttgcttctg ccttggaggt tttgaaagtc gttgagaaga agcgacctga gttaaaactc
     3181 gagtttgaag aacacaagat tggaggtgcc tctattgatg cctatggaac ccctttgact
     3241 gacgagactg tgaaggcttg tttggaagct gacggtgttc ttttgggtgc cgttggtggt
     3301 cctgaatgga ccaaccccaa ttgtcgtcct gagcaaggtt tattgaagct tcgtaagagt
     3361 atgggtgttt gggccaacct tcgaccttgc aactttgcca gcaagtcttt agtcaagtac
     3421 agccctttga agcctgaaat cgttgaaggt gtcgattttt gtgttgtacg agaacttact
     3481 ggaggttgtt actttggtga gcgcactgag gacaacggat cgggttatgc tatggacact
     3541 tggccttaca gtttggaaga agtttctcgt attgctcgtt tggcggccgc gggcccaaca
     3601 ccaatgttta taaccaagtt ttatcttgtt tgtctacatg gtattttaca ttcatctaca
     3661 tacatctttc attggctttg tacatagtta tcattacaag tctaaaaaaa ttcactcttt
     3721 tcttattcaa tgtcaatcca agagaaaaga ttgtggtaat gttgtaggag catgtttaat
     3781 aaattactat agcaaattac tttttattcc caaggtgttt atctataata gttaatattt
     3841 tagtcgctac ataaaatttt accaaagagt acttgtatac taattctaaa tgccttctga
     3901 cataaaacgc ctaggaaaac aaacgcaaac aaggcatcga ctttttcaat aaccaaccaa
     3961 aaaaatttta cattagtctt tttttaatgc tgagaaagtc tttgctgata tgccttccaa
     4021 ccagcttctc tatatctctt ggcttcgaca acaggattac gaccagctcc atagactcca
     4081 cgaccaacaa tgatgatgtc gctaccgcag tttacaatca cttcttcagg agtacgatat
     4141 tgctgtccca gcccgtctcc tttaacatcc aagccgatac caggggacat agttatgtag
     4201 tcgctttgaa ggttaggaaa tcgacgacca gctataaagc caaagcaaaa atcggtatgc
     4261 ttctcaaacc attctaaggt tttctctgtg taggaaccag tagccaaaga gcctttggaa
     4321 gacatttcag ccaaaagcaa gagaccacgt cccaaaggta aaccaacttc tttgaggcct
     4381 tgtataatac cctcgcctgg cactgtatgg caatttgtga tatgagccca agaagcaatt
     4441 ttgtacacac cagatgcata ttgtagcttg acggtatttc caatgtctgc gaatttgcga
     4501 tcctcaaaga taagaaaacg atgcttttta cctaaggcca ccagtttttc taccatatcc
     4561 tggtcgaaat cctcgacaac gtcaatatgt gtcttgataa cacagacata gggtccaatt
     4621 ttatctacca attctaagat ttcggatttc ttcgtcaaat cgaccgcgac tgacaagttg
     4681 ctttgctttt cttccatcaa agccaacaat tccttggcaa tgggattttt catcccctca
     4741 gctctagctg aatagctttg aaatactcta gcatccataa ctttgctttt aaacctttaa
     4801 tttcgatcca agcaaaaaag aggttcttgg taggacaata cggtaagaaa acacgacatg
     4861 tgcagagatg ccgacgaagc atagttaaac tgggatggta aaatcaatta agaatttata
     4921 aagacaaaat tgtataagtc tctaaaacat cttaattata cctcacagaa ctatctaaaa
     4981 tatattcaca aagtgcaaac attatcatga aaaagaacca ttttaattta aagcaagggc
     5041 attaaggctt atttacagaa tttcttactt ttgtaaagat tataaggctg attatctttt
     5101 tcaccatgcc aaaaattaca caagatagaa tggatgtttg aaattaaacg tgagtataca
     5161 aacaaataca ctaggtaaat cgaaacattt ttttctccat taagtaacaa attcctattt
     5221 agagaaagaa tgctgagtag attaaataat ctatacaaac ttttttaaca caaatgcata
     5281 catatagcca gtgggatttg tagctaagct tgcgaattcg agtctaactc cttaaccact
     5341 cggacatagt gacttatctg acactcattg taaattaata tatatatagg cattttgttt
     5401 agttaaaggt acttaagtaa ttagtataaa cgaaccaatt ttataatcag gaagttaagt
     5461 gaatggtagc acatgtcgta aaaattgtga atttttattg aataatattt taaatacaag
     5521 cctttctaga ctaggtatac tcataaacat atatgagcaa aaggatagag gagattacat
     5581 tgcatcttct acaaatttat ttattgccct ttactgaaaa attaaataat gagtactaaa
     5641 tgataaaaag cgctcagtac aagaaagatt gcaaaaatat tgcattcttc atgaattaaa
     5701 gttgcatata aggcatattg aaagtaatag tactaaaaca gcagttagcg aaaattaata
     5761 gaattatatt cgcaagacaa ttgtgacatt aaattaaaaa attgtagaat ttttactatc
     5821 ctctttaacg ccatgagcct ttataaaaag gttaaattag ttttaacatt ctttttttga
     5881 gtaagagttt acaatttatc aaaacctgtg ttattatatt cattagtttc aatttattag
     5941 catctagaga aaaatcaatt ggcagttaca ttgttggaat ttatgaagaa aaagaatcta
     6001 caacggagaa taagttgctg atcgctttcc ctaaaattgt atattttgct gagcttattt
     6061 tgacatttcg ttgaagtttt ctctaattcg cattcatttt aagtaaaaca atgagaaata
     6121 aaattacaaa aaatacaaat taaaatacaa tttttagcta taattataga cgatgccctt
     6181 gtatcccatt ctgtctcgct tgcccctact ttttatcttt tatataccat aatgaacgct
     6241 gccgctacta accatacccc gattttacat ttcggactcc caaggacgta caaaatagaa
     6301 aactatagaa aaaaataatc agaaaatagc atgtcatctc tttgtaaaac gcgtttgcaa
     6361 gaagaaagga aacaatggag aagagatcat ccatttgtat gtaaatttta gtaaacttga
     6421 agaaatcact aacaacttct cttacttagg gattctatgc aaaaccttgt aaatcatctg
     6481 atggaggact cgatttaatg aattggaagg ttgggcccgc ggccgcttag ctgaaacttc
     6541 caaccctcct gctcccgtca cattactcga caaagctaat gttttggcaa cttctcgtct
     6601 ttggcgcaag actgtagcta agatttttaa ggaggaatat cctcacctta ccttaaaaaa
     6661 ccagctcatt gactccgcag ccatgctttt ggtcaagagc cctcgtacac ttaacggtgt
     6721 tgttttgact gacaacttgt ttggtgacat tatttcagat gaggcttctg tcattcctgg
     6781 tagcttgggc cttttgcctt ctgcctccct ttccggtgtg gtaggaaaat cagaagaaaa
     6841 ggttcattgt ttggttgagc ccattcacgg tagcgctccc gagctcgaat tcactggccg
     6901 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6961 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7021 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     7081 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     7141 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     7201 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7261 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7321 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7381 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7441 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     7501 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7561 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7621 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7681 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7741 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7801 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7861 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7921 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7981 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8041 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8101 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8161 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8221 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8281 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8341 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8401 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8461 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8521 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8581 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8641 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8701 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8761 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8821 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8881 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9121 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9661 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9721 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9781 accgcctctc cccgcgcgtt ggccgattca ttaatgc
//
